Dr Thomas Roberts

BScAgr PhD Sydney
Senior Lecturer in Food Chemistry and Biotechnology
Dept of Plant and Food Science

C81 - ATP - The Biomedical Building
The University of Sydney

Telephone +61 2 8627 1042
Fax +61 2 8627 1099

Biographical details

Tom Roberts took up a Senior Lectureship at the University of Sydney in January 2012. He has a passion for understanding the properties and functions of plant proteins, as well as their functionality in foods. He heads a research group working on several projects on a range of species including Chlamydomonas, Arabidopsis, barley, sorghum and peanut. Roberts has 11 years’ experience teaching a broad range of undergraduate units within the molecular biosciences.

After studying at James Ruse Agricultural High School in Sydney, Roberts completed a BScAgr degree at the University of Sydney in 1988, majoring in Agricultural Chemistry. He then received an Alexander Hugh Thurburn PhD scholarship and graduated with a PhD thesis entitled “Glyoxylate aminotransferases and ureide catabolism in the developing fruits of legumes” in 1995.

Following his PhD research, Roberts worked as a Postdoc at Lund University, Sweden (1994-5) on the properties and functions of the multiple NAD(P)H dehydrogenases in plant mitochondria. Back in Sydney he developed novel methods for the purification of pharmaceutical-grade lactose from cheese whey at the University of Western Sydney/CSIRO (1995-6). He returned to Scandinavia and worked as a technical editor at Lund University and at the Royal Agricultural and Veterinary University in Denmark, as well as a Research Scientist at Novartis Seeds in Sweden. He then completed a 3-year Research Fellowship (1997-2000) at the Risø National Laboratory and the Technical University of Denmark on the properties and functions of serpins in barley and wheat.

From late 2000, Roberts worked as a Research Fellow in the Macquarie University Centre for Analytical Biotechnology and conducted research on the mechanism, structure and regulation of magnesium chelatase, a key enzyme in chlorophyll biosynthesis. In 2004 he accepted an ongoing lectureship at Macquarie University. Roberts taught a range of units there including 2nd-year Plant Structure and Function, 3rd-year Cell and Developmental Biology and 3rd-year Plants: Cells and Molecules. Roberts was a Key Researcher in the Grain Foods CRC (2004-9).

Research interests

Tom Roberts’ research concerns a variety of plant proteins, their properties, roles in the plant, and functionality in foods. His major interest for many years has been the structure, properties and functions of the serpin family of proteins in plants. He also has interests in peanut allergens, responses of plants to an energy crisis, the chemistry and biochemistry of sorghum grain, wheat rust, and plant gene silencing.

Most serpins are potent, irreversible inhibitors of specific serine or cysteine proteases. Serpins are fascinating proteins from many perspectives and have a remarkable variety of functions in animals. The structures and functions of plant and algal serpins remain largely unknown but some breakthroughs have been made in recent years - see Ahn et al. (2009) and Lampl et al. (2010) listed below, as well as Lampl et al. (2013; doi: 10.1111/tpj.12141). Roberts’ current work focuses on serpins in the unicellular green alga, Chlamydomonas, in Arabidopsis, in barley and wheat.

Another of Roberts’ research interests is the allergenicity of peanuts. Peanut allergy is a major problem in Australia and many other countries; indeed some people (including children) can suffer life-threatening anaphylactic shock upon ingestion of peanuts, even extremely small amounts. The allergens in peanut kernels are seed storage proteins but what determines their absolute and relative abundance in the mature peanut? See Roberts’ recent paper (Walczyk et al., 2013) for one answer to this question.

Roberts is also interested is the response of plants to an energy crisis. The model system for this work is the rice coleoptile, the sheath that protects the growing shoot from a germinated rice grain. Using oxygen deprivation - either hypoxia (low oxygen) or anoxia (no oxygen) - an energy crisis can be induced during growth of the coleoptile. Responses studied include energy allocation, transcription (microarrays) and translation (proteomics). See Roberts’ recent paper (Edwards et al., 2012) for an analysis of energy production and allocation in rice coleoptiles under oxygen deprivation.

One of Roberts’ more recent research interests is in the chemistry and biochemistry of sorghum grain, particularly concerning the availability of starch in animal feed and the effects of malting.

To pursue his interests, Roberts’ research involves a broad range of techniques in plant physiology, cell biology, molecular biology and protein biochemistry. Techniques employed recently include radiolabelling, confocal microscopy, microarray analysis, RT-PCR and 2D-DIGE. Bioinformatics analysis is involved in many aspects of Roberts’ projects.

Please contact Tom Roberts if you are interested in pursuing a PhD in one of the above topics.

Teaching and supervision

In 2014 Tom Roberts is joint coordinator (with Tina Bell) of AGEN2001 Plant Function, and coordinator of AGCH3025 Chemistry and Biochemistry of Foods. He is teaching one of four modules in AGCH3026 Food Biotechnology, as well as part of the Masters unit, AFNR5107 Principles of Biochemical Analysis. He is the Postgraduate Coordinator, Honours Coordinator and Seminar Coordinator for the Department of Plant and Food Sciences.

Roberts is Research Supervisor for Ali Khoddami (sorghum grain chemistry and biochemistry), Nicolas Daneri (programmed cell death in barley germination) and Rodrigo Sequeira Reis (DRB proteins in plant gene silencing). His 2013 BScAgr 4th-year research student, Shi-Hwee Chan (optimization of starch extraction from sorghum grain) was awarded the University Medal. Roberts’ most recent PhD completion, Nicole Walczyk (effects of plant environment on peanut allergenicity), is now a Marie Curie Research Fellow in Portugal. His most recent Honours student Julian May (serpins in rice coleoptile senescence) at Macquarie University (MQ), received 1st-class Honours and is now a PhD student at MQ. Roberts was Associate Supervisor for a PhD student at MQ, Joshua Edwards, who was recently awarded his PhD on the effects of oxygen deprivation on rice coleoptiles. Roberts is Auxiliary Supervisor for University of Sydney PhD student Najeeb Ullah (responses of cotton to flooding; supervised by Daniel Tan), Janet Basani (auxin response factors in Arabidopsis; supervised by Brian Jones) and Mary Otieno (desiccation tolerance in Rhizobia; supervised by Roz Deaker).

Current projects

GRANTS

Selle PH and Roberts TH. The factors influencing sorghum starch digestibility in broiler chickens. Rural Industries Research and Development Corporation (RIRDC). $130K. 30/07/2013 to 30/07/2015

Themes

Plant and crop physiology; Food Production, Quality and Safety

Selected grants

2010

  • Do plant growth conditions affect allergen concentrations in peanuts?; Roberts T; Macquarie University/Linkage Project Scheme Grant.

2009

  • Functional analysis of plant serpins; Roberts T; Macquarie University/Research Development Grant.

Selected publications

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Book Chapters

  • Roberts, T., Ahn, J., Lampl, N., Fluhr, R. (2011). Plants and the Study of Serpin Biology. In Whisstock JC and Bird PI (Eds.), Methods in Enzymology Volume 499, (pp. 347-366). San Diego: Academic Press.
  • Hejgaard, J., Roberts, T. (2007). Plant Serpins. In Silverman GA and Lomas DA (Eds.), Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, (pp. 279-300). New Jersey: World Scientific Publishing.

Journals

  • Liu, S., Selle, P., Khoddami, A., Roberts, T., Cowieson, A. (2014). Graded inclusions of sodium metabisulphite insorghum-based diets: II. Modification of starch pastingproperties in vitro and beneficial impacts on starch digestiondynamics in broiler chickens. Animal Feed Science and Technology, 190, 68-78. [More Information]
  • Naz, R., Bano, A., Wilson, N., Guest, D., Roberts, T. (2014). Pathogenesis-Related Protein Expression in the Apoplast of Wheat Leaves Protected Against Leaf Rust Following Application of Plant Extracts. Phytopathology, 104(9), 933-944.
  • Khoddami, A., Che Man, Y., Roberts, T. (2014). Physico�?chemical properties and fatty acid profile of seed oils from pomegranate (Punica granatum L.) extracted by cold pressing. European Journal of Lipid Science and Technology, 116(5), 553-562. [More Information]
  • Walczyk, N., Smith, P., Tovey, E., Wright, G., Fleischfresser, D., Roberts, T. (2013). Analysis of crude protein and allergen abundance in peanuts (arachis hypogaea cv. walter) from three growing regions in Australia. Journal of Agricultural and Food Chemistry, 61(15), 3714-3725. [More Information]
  • Khoddami, A., Wilkes, M., Roberts, T. (2013). Techniques for Analysis of Plant Phenolic Compounds. Molecules, 18(2), 2328-2375. [More Information]
  • Vella, N., Joss, T., Roberts, T. (2012). Chilling-induced ultrastructural changes to mesophyll cells of Arabidopsis grown under short days are almost completely reversible by plant re-warming. Protoplasma, 249, 1137-1149. [More Information]
  • Edwards, J., Roberts, T., Atwell, B. (2012). Quantifying ATP turnover in anoxic coleoptiles of rice (Oryza sativa) demonstrates preferential allocation of energy to protein synthesis. The Journal of Experimental Biology, 63(12), 4389-4402. [More Information]
  • Fluhr, R., Lampl, N., Roberts, T. (2012). Serpin protease inhibitors in plant biology. Physiologia Plantarum, 145(1), 95-102. [More Information]
  • Francis, S., Ersoy, R., Ahn, J., Atwell, B., Roberts, T. (2012). Serpins in rice: protein sequence analysis, phylogeny and gene expression during development. BMC Genomics, 13(1), 1-17. [More Information]
  • Lampl, N., Budai-Hadrian, O., Davydov, O., Joss, T., Harrop, S., Curmi, P., Roberts, T., Fluhr, R. (2010). Arabidopsis AtSerpin1: crystal structure and in vivo interaction with its target protease RESPONSIVE TO DESICCATION-21 (RD21). Journal of Biological Chemistry, 285(18), 13550-13560. [More Information]
  • Jerkovic, A., Kriegel, A., Bradner, J., Atwell, B., Roberts, T., Willows, R. (2010). Strategic Distribution of Protective Proteins within Bran Layers of Wheat Protects the Nutrient-Rich Endosperm. Plant Physiology, 152(3), 1459-1470.
  • Peterson, R., Bradner, J., Roberts, T., Nevalainen, H. (2009). Fungi from koala (Phascolarctos cinereus) faeces exhibit a broad range of enzyme activities against recalcitrant substrates. Letters in Applied Microbiology, 48(2), 218-225. [More Information]
  • Mak, Y., Willows, R., Roberts, T., Wrigley, C., Sharp, P., Copeland, L. (2009). Germination of Wheat: A Functional Proteomics Analysis of the Embryo. Cereal Chemistry, 86(3), 281-289. [More Information]
  • Ahn, J., Atwell, B., Roberts, T. (2009). Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA alkylating agent in Arabidopsis. BMC Plant Biology, 9, 1-11. [More Information]
  • Roberts, T., Hejgaard, J. (2008). Serpins in plants and green algae. Functional and Integrative Genomics, 8(1), 169-27. [More Information]
  • Haynes, P., Roberts, T. (2007). Subcellular shotgun proteomics in plants: looking beyond the usual suspects. Proteomics. Proteomics, 7(16), 2963-2975. [More Information]
  • Mak, Y., Willows, R., Roberts, T., Wrigley, C., Sharp, P., Copeland, L. (2006). Black Point is associated with reduced levels of stress, disease- and defence-related proteins in wheat grain. Molecular Plant Pathology, 7(3), 177-189.
  • Hejgaard, J., Laing, W., Marttila, S., Gleave, A., Roberts, T. (2005). Serpins in fruit and vegetative tissues of apple (Malus domestica): expression of four serpins with distinct reactive centres and characterization of a major inhibitory seed form, MdZ1b. Functional Plant Biology: an international journal of plant function, 32(6), 517-527. [More Information]
  • Austin, C., Mizdrak, J., Matin, A., Sirijovski, N., Kosim-Satyaputra, P., Willows, R., Roberts, T., Truscott, R., Polekhina, G., Parker, M., et al (2004). Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase. Protein Expression and Purification, 37, 392-398.
  • Roberts, T., Hejgaard, J., Saunders, N., Cavicchioli, R., Curmi, P. (2004). Serpins in Unicellular Eukarya, Archaea, and Bacteria: Sequence Analysis and Evolution. Journal of Molecular Evolution, 59(4), 437-447. [More Information]
  • Roberts, T., Marttila, S., Rasmussen, S., Hejgaard, J. (2003). Differential gene expression for suicide-substrate serine proteinase inhibitors (serpins) in vegetative and grain tissues of barley. Journal of Experimental Botany, 54(391), 2251-2263. [More Information]
  • Willows, R., Lake, V., Roberts, T., Beale, S. (2003). Inactivation of Mg Chelatase during Transition from Anaerobic to Aerobic Growth in Rhodobacter capsulatus. Journal of Bacteriology, 185(11), 3249-3258. [More Information]
  • Hansson, A., Willows, R., Roberts, T., Hansson, M. (2002). Three semidominant barley mutants with single amino acid substitutions in the smallest magnesium chelatase subunit form defective AAA+ hexamers. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 99(21), 13944-13949. [More Information]
  • Ostergaard, H., Rasmussen, S., Roberts, T., Hejgaard, J. (2000). Inhibitory Serpins from Wheat Grain with Reactive Centers Resembling Glutamine-rich Repeats of Prolamin Storage Proteins: Cloning and Characterization of Five Major Molecular Forms. Journal of Biological Chemistry, 275(43), 33272-33279. [More Information]
  • Roberts, T., Lees, E. (1997). Glyoxylate aminotransferase activities in developing fruits of legumes. Journal of Biochemistry, Molecular Biology and Biophysics, 1, 63-71.
  • Roberts, T., Fredlund, K., Moller, I. (1996). Direct evidence for the presence of two external NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria. FEBS Letters, 373, 307-309.
  • Melo, A., Roberts, T., Moller, I. (1996). Evidence for the presence of two rotenone-insensitive internal NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria. Biochimica et Biophysica Acta-Bioenergetics, 1276, 133-139.
  • Roberts, T., Rasmusson, A., Moller, I. (1996). Platanetin and 7-iodo-acridone-4-carboxylic acid are not specific inhibitors of respiratory NAD(P)H dehydrogenases in potato tuber mitochondria. Physiologia Plantarum, 96, 263-267.
  • Moller, I., Roberts, T., Rasmusson, A. (1996). Ubiquinone-1 lnduces Externa1 Deamino-NADH Oxidation in Potato Tuber Mitochondria. Plant Physiology, 112(1), 75-78.

Conferences

  • Liu, S., Khoddami, A., Roberts, T., Selle, P. (2014). Phenolic Compounds and Phytate Influence Starch and Protein Digestion Rates in Sorghum-based Broiler Diets. 25th Annual Australian Poultry Science Symposium, Sydney: University of Sydney.
  • Roberts, T., Lampl, N., Curmi, P., Fluhr, R. (2013). AtSerpin1, an inhibitor in vivo of the papain-like cysteine protease RD21. ICAR2013: 24th International Conference on Arabidopsis Research, Sydney, Australia.
  • Khoddami, A., Wilkes, M., Roberts, T. (2013). Effect of malting on phenolic compounds and antioxidant activity in four Australian sorghums. ICC Conference 2013 in Association with the 63rd Australian Cereal Chemistry Conference, Australia: International Association for Cereal Science and Technology.
  • Edwards, J., Roberts, T., Atwell, B. (2012). Preferential allocation of energy to protein synthesis in rice coleoptiles under oxygen deprivation. ComBio2012, Adelaide, SA.
  • Roberts, T., Lampl, N., Davydov, O., Joss, T., Harrop, S., Curmi, P., Fluhr, R. (2011). AtSerpin1 from Arabidopsis: X-ray crystal structure and identification of RD21, a papain-like cysteine protease, as the major target. 6th International Symposium on the Chemistry and Biology of Serpins, Chapel Hill, North Carolina, USA.
  • Roberts, T., Joss, T., Lampl, N., Fluhr, R., Harrop, S., Curmi, P. (2011). Structure, mechanism and inhibitory specificity of the serpin family of protease inhibitors in plants. 1st International Conference on Plant Proteases, Hemavan, Sweden.
  • Edwards, J., Roberts, T., Atwell, B. (2010). Surviving an energy crisis: ATP usage in anoxic rice coleoptiles is dominated by protein synthesis. ComBio2010, Melbourne, Australia.
  • Walczyk, N., Smith, P., Tovey, E., Beggs, P., Roberts, T. (2010). The impact of climate change on the allergenicity of peanuts. 4th Annual Meeting of EuroBAT, Munich, Germany.
  • Walczyk, N., Smith, P., Tovey, E., Beggs, P., Roberts, T. (2010). The impact of climate change on the allergenicity of peanuts. 4th International Symposium of Molecular Allergology (ISMA), Munich, Germany.
  • Walczyk, N., Tovey, E., Smith, P., Beggs, P., Roberts, T. (2010). The impact of climate change on the allergenicity of peanuts. 4th International Symposium of the 'Sonderforschungsbereich 429', Potsdam, Germany.
  • Budai-Hadrian, O., Lampl, N., Davydov, O., Miersch, O., Vasternack, C., Joss, T., Harrop, S., Curmi, P., Roberts, T., Fluhr, R. (2009). Regulatory role in defence and crystal structure of the Arabidopsis protease inhibitor AtSerpin1. ComBio2009, Christchurch, NZ.
  • Roberts, T., Marttila, S., Rasmussen, S., Hejgaard, J. (2000). Serpins in vegetative and seed tissues of barley. ComBio2000, Wellington, New Zealand.
  • Roberts, T., Lees, E. (1992). Glyoxylate aminotransferases in developing fruits of french bean (Phaseolus vulgaris). 32nd Annual General Meeting of the Australian Society of Plant Physiologists, Melbourne.

Other

  • Roberts, T. (1995), Glyoxylate aminotransferases and ureide catabolism in the developing fruits of legumes (PhD thesis).
  • Roberts, T. (1989), Biochemical response of cabbage seedlings to high levels of copper (BScAgr (Hons) thesis).

2014

  • Liu, S., Selle, P., Khoddami, A., Roberts, T., Cowieson, A. (2014). Graded inclusions of sodium metabisulphite insorghum-based diets: II. Modification of starch pastingproperties in vitro and beneficial impacts on starch digestiondynamics in broiler chickens. Animal Feed Science and Technology, 190, 68-78. [More Information]
  • Naz, R., Bano, A., Wilson, N., Guest, D., Roberts, T. (2014). Pathogenesis-Related Protein Expression in the Apoplast of Wheat Leaves Protected Against Leaf Rust Following Application of Plant Extracts. Phytopathology, 104(9), 933-944.
  • Liu, S., Khoddami, A., Roberts, T., Selle, P. (2014). Phenolic Compounds and Phytate Influence Starch and Protein Digestion Rates in Sorghum-based Broiler Diets. 25th Annual Australian Poultry Science Symposium, Sydney: University of Sydney.
  • Khoddami, A., Che Man, Y., Roberts, T. (2014). Physico�?chemical properties and fatty acid profile of seed oils from pomegranate (Punica granatum L.) extracted by cold pressing. European Journal of Lipid Science and Technology, 116(5), 553-562. [More Information]

2013

  • Walczyk, N., Smith, P., Tovey, E., Wright, G., Fleischfresser, D., Roberts, T. (2013). Analysis of crude protein and allergen abundance in peanuts (arachis hypogaea cv. walter) from three growing regions in Australia. Journal of Agricultural and Food Chemistry, 61(15), 3714-3725. [More Information]
  • Roberts, T., Lampl, N., Curmi, P., Fluhr, R. (2013). AtSerpin1, an inhibitor in vivo of the papain-like cysteine protease RD21. ICAR2013: 24th International Conference on Arabidopsis Research, Sydney, Australia.
  • Khoddami, A., Wilkes, M., Roberts, T. (2013). Effect of malting on phenolic compounds and antioxidant activity in four Australian sorghums. ICC Conference 2013 in Association with the 63rd Australian Cereal Chemistry Conference, Australia: International Association for Cereal Science and Technology.
  • Khoddami, A., Wilkes, M., Roberts, T. (2013). Techniques for Analysis of Plant Phenolic Compounds. Molecules, 18(2), 2328-2375. [More Information]

2012

  • Vella, N., Joss, T., Roberts, T. (2012). Chilling-induced ultrastructural changes to mesophyll cells of Arabidopsis grown under short days are almost completely reversible by plant re-warming. Protoplasma, 249, 1137-1149. [More Information]
  • Edwards, J., Roberts, T., Atwell, B. (2012). Preferential allocation of energy to protein synthesis in rice coleoptiles under oxygen deprivation. ComBio2012, Adelaide, SA.
  • Edwards, J., Roberts, T., Atwell, B. (2012). Quantifying ATP turnover in anoxic coleoptiles of rice (Oryza sativa) demonstrates preferential allocation of energy to protein synthesis. The Journal of Experimental Biology, 63(12), 4389-4402. [More Information]
  • Fluhr, R., Lampl, N., Roberts, T. (2012). Serpin protease inhibitors in plant biology. Physiologia Plantarum, 145(1), 95-102. [More Information]
  • Francis, S., Ersoy, R., Ahn, J., Atwell, B., Roberts, T. (2012). Serpins in rice: protein sequence analysis, phylogeny and gene expression during development. BMC Genomics, 13(1), 1-17. [More Information]

2011

  • Roberts, T., Lampl, N., Davydov, O., Joss, T., Harrop, S., Curmi, P., Fluhr, R. (2011). AtSerpin1 from Arabidopsis: X-ray crystal structure and identification of RD21, a papain-like cysteine protease, as the major target. 6th International Symposium on the Chemistry and Biology of Serpins, Chapel Hill, North Carolina, USA.
  • Roberts, T., Ahn, J., Lampl, N., Fluhr, R. (2011). Plants and the Study of Serpin Biology. In Whisstock JC and Bird PI (Eds.), Methods in Enzymology Volume 499, (pp. 347-366). San Diego: Academic Press.
  • Roberts, T., Joss, T., Lampl, N., Fluhr, R., Harrop, S., Curmi, P. (2011). Structure, mechanism and inhibitory specificity of the serpin family of protease inhibitors in plants. 1st International Conference on Plant Proteases, Hemavan, Sweden.

2010

  • Lampl, N., Budai-Hadrian, O., Davydov, O., Joss, T., Harrop, S., Curmi, P., Roberts, T., Fluhr, R. (2010). Arabidopsis AtSerpin1: crystal structure and in vivo interaction with its target protease RESPONSIVE TO DESICCATION-21 (RD21). Journal of Biological Chemistry, 285(18), 13550-13560. [More Information]
  • Jerkovic, A., Kriegel, A., Bradner, J., Atwell, B., Roberts, T., Willows, R. (2010). Strategic Distribution of Protective Proteins within Bran Layers of Wheat Protects the Nutrient-Rich Endosperm. Plant Physiology, 152(3), 1459-1470.
  • Edwards, J., Roberts, T., Atwell, B. (2010). Surviving an energy crisis: ATP usage in anoxic rice coleoptiles is dominated by protein synthesis. ComBio2010, Melbourne, Australia.
  • Walczyk, N., Smith, P., Tovey, E., Beggs, P., Roberts, T. (2010). The impact of climate change on the allergenicity of peanuts. 4th Annual Meeting of EuroBAT, Munich, Germany.
  • Walczyk, N., Smith, P., Tovey, E., Beggs, P., Roberts, T. (2010). The impact of climate change on the allergenicity of peanuts. 4th International Symposium of Molecular Allergology (ISMA), Munich, Germany.
  • Walczyk, N., Tovey, E., Smith, P., Beggs, P., Roberts, T. (2010). The impact of climate change on the allergenicity of peanuts. 4th International Symposium of the 'Sonderforschungsbereich 429', Potsdam, Germany.

2009

  • Peterson, R., Bradner, J., Roberts, T., Nevalainen, H. (2009). Fungi from koala (Phascolarctos cinereus) faeces exhibit a broad range of enzyme activities against recalcitrant substrates. Letters in Applied Microbiology, 48(2), 218-225. [More Information]
  • Mak, Y., Willows, R., Roberts, T., Wrigley, C., Sharp, P., Copeland, L. (2009). Germination of Wheat: A Functional Proteomics Analysis of the Embryo. Cereal Chemistry, 86(3), 281-289. [More Information]
  • Budai-Hadrian, O., Lampl, N., Davydov, O., Miersch, O., Vasternack, C., Joss, T., Harrop, S., Curmi, P., Roberts, T., Fluhr, R. (2009). Regulatory role in defence and crystal structure of the Arabidopsis protease inhibitor AtSerpin1. ComBio2009, Christchurch, NZ.
  • Ahn, J., Atwell, B., Roberts, T. (2009). Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA alkylating agent in Arabidopsis. BMC Plant Biology, 9, 1-11. [More Information]

2008

  • Roberts, T., Hejgaard, J. (2008). Serpins in plants and green algae. Functional and Integrative Genomics, 8(1), 169-27. [More Information]

2007

  • Hejgaard, J., Roberts, T. (2007). Plant Serpins. In Silverman GA and Lomas DA (Eds.), Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, (pp. 279-300). New Jersey: World Scientific Publishing.
  • Haynes, P., Roberts, T. (2007). Subcellular shotgun proteomics in plants: looking beyond the usual suspects. Proteomics. Proteomics, 7(16), 2963-2975. [More Information]

2006

  • Mak, Y., Willows, R., Roberts, T., Wrigley, C., Sharp, P., Copeland, L. (2006). Black Point is associated with reduced levels of stress, disease- and defence-related proteins in wheat grain. Molecular Plant Pathology, 7(3), 177-189.

2005

  • Hejgaard, J., Laing, W., Marttila, S., Gleave, A., Roberts, T. (2005). Serpins in fruit and vegetative tissues of apple (Malus domestica): expression of four serpins with distinct reactive centres and characterization of a major inhibitory seed form, MdZ1b. Functional Plant Biology: an international journal of plant function, 32(6), 517-527. [More Information]

2004

  • Austin, C., Mizdrak, J., Matin, A., Sirijovski, N., Kosim-Satyaputra, P., Willows, R., Roberts, T., Truscott, R., Polekhina, G., Parker, M., et al (2004). Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase. Protein Expression and Purification, 37, 392-398.
  • Roberts, T., Hejgaard, J., Saunders, N., Cavicchioli, R., Curmi, P. (2004). Serpins in Unicellular Eukarya, Archaea, and Bacteria: Sequence Analysis and Evolution. Journal of Molecular Evolution, 59(4), 437-447. [More Information]

2003

  • Roberts, T., Marttila, S., Rasmussen, S., Hejgaard, J. (2003). Differential gene expression for suicide-substrate serine proteinase inhibitors (serpins) in vegetative and grain tissues of barley. Journal of Experimental Botany, 54(391), 2251-2263. [More Information]
  • Willows, R., Lake, V., Roberts, T., Beale, S. (2003). Inactivation of Mg Chelatase during Transition from Anaerobic to Aerobic Growth in Rhodobacter capsulatus. Journal of Bacteriology, 185(11), 3249-3258. [More Information]

2002

  • Hansson, A., Willows, R., Roberts, T., Hansson, M. (2002). Three semidominant barley mutants with single amino acid substitutions in the smallest magnesium chelatase subunit form defective AAA+ hexamers. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 99(21), 13944-13949. [More Information]

2000

  • Ostergaard, H., Rasmussen, S., Roberts, T., Hejgaard, J. (2000). Inhibitory Serpins from Wheat Grain with Reactive Centers Resembling Glutamine-rich Repeats of Prolamin Storage Proteins: Cloning and Characterization of Five Major Molecular Forms. Journal of Biological Chemistry, 275(43), 33272-33279. [More Information]
  • Roberts, T., Marttila, S., Rasmussen, S., Hejgaard, J. (2000). Serpins in vegetative and seed tissues of barley. ComBio2000, Wellington, New Zealand.

1997

  • Roberts, T., Lees, E. (1997). Glyoxylate aminotransferase activities in developing fruits of legumes. Journal of Biochemistry, Molecular Biology and Biophysics, 1, 63-71.

1996

  • Roberts, T., Fredlund, K., Moller, I. (1996). Direct evidence for the presence of two external NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria. FEBS Letters, 373, 307-309.
  • Melo, A., Roberts, T., Moller, I. (1996). Evidence for the presence of two rotenone-insensitive internal NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria. Biochimica et Biophysica Acta-Bioenergetics, 1276, 133-139.
  • Roberts, T., Rasmusson, A., Moller, I. (1996). Platanetin and 7-iodo-acridone-4-carboxylic acid are not specific inhibitors of respiratory NAD(P)H dehydrogenases in potato tuber mitochondria. Physiologia Plantarum, 96, 263-267.
  • Moller, I., Roberts, T., Rasmusson, A. (1996). Ubiquinone-1 lnduces Externa1 Deamino-NADH Oxidation in Potato Tuber Mitochondria. Plant Physiology, 112(1), 75-78.

1995

  • Roberts, T. (1995), Glyoxylate aminotransferases and ureide catabolism in the developing fruits of legumes (PhD thesis).

1992

  • Roberts, T., Lees, E. (1992). Glyoxylate aminotransferases in developing fruits of french bean (Phaseolus vulgaris). 32nd Annual General Meeting of the Australian Society of Plant Physiologists, Melbourne.

1989

  • Roberts, T. (1989), Biochemical response of cabbage seedlings to high levels of copper (BScAgr (Hons) thesis).

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