Dr Ben Crossett

Research Fellow
School of Molecular Bioscience

G08 - Biochemistry Building
The University of Sydney
NSW 2006 Australia

T: +61 2 9351 6010
F: +61 2 9351 5858
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W: Related website

Biographical details

Dr Crossett began his scientific career at the University of Manchester, gaining a BSc(Hons) in Biochemistry, followed by a PhD at the University of Cambridge under the mentorship of Dr F Stewart, in which he characterised a novel uterine protein in mares. He completed post-doctoral research at Imperial College, London investigating protein based tumour therapies and subsequently utilised a proteomics-based approach to discover novel anti-microbial and vaccine targets in Burkholderia pseudomallei. In 2004, he became the Research Fellow in Proteomics and Manager of the Sydney University Proteome Research Unit.

Research interests

Dr Ben Crossett is the manager of the Sydney University Proteome Research Unit (SUPRU). The facility specialises in the application of 2D Gel Electrophoresis/DIGE, shotgun proteomics techniques/iTRAQ and selective reaction monitoring to the study of biological systems from a wide range of species from alpacas to zoozanthella. SUPRU provides a range of contract research services and training for researchers wishing to access the state-of-the-art equipment.

As manager of the SUPRU he is involved in a wide range of projects, however his primary research focus is understanding of the molecular process driving the formation and repair of multiple sclerosis lesions and ultimately the failure of this repair mechanism in most patients. Through this work his research group have developed unparallel expertise in processing (particularly laser guided microdissection) of formalin fixed neurological tissue for proteomics applications.

Publications

2013 | 2012 | 2011 | 2010 | 2008

2013

   
  • Druart, X., Rickard, J., Mactier, S., Kohnke, P., Kershaw-Young, C., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Harichaux, G., Grupen, C., de Graaf, S. (2013), Proteomic characterization and cross species comparison of mammalian seminal plasma. Journal of Proteomics. 91, 13-22. [Abstract]
  • Wang, S., Hassani, M., Crossett, B., Copeland, L. (2013), Extraction and identification of internal granule proteins from waxy wheat starch. Starch. 65(1-2), 186-190.
  • Van Ly, D., Faiz, A., Jenkins, C., Crossett, B., Black, J., McParland, B., Burgess, J., Oliver, B. (2013), Characterising the mechanism of airway smooth muscle β2 adrenoceptor desensitization by rhinovirus infected bronchial epithelial cells. PloS One. 8(2), e56058. [Abstract]

2012

   
  • Kohnke, P., Mactier, S., Almazi, J., Crossett, B., Christopherson, R. (2012), Fludarabine and Cladribine Induce Changes in Surface Proteins on Human B-Lymphoid Cell Lines Involved with Apoptosis, Cell Survival, and Antitumor Immunity. Journal of Proteome Research. 11(9), 4436-4448. [Abstract]
  • Leong, S., Nunez, A., Lin, M., Crossett, B., Christopherson, R., Baxter, R. (2012), iTRAQ-Based Proteomic Profiling of Breast Cancer Cell Response to Doxorubicin and TRAIL. Journal of Proteome Research. 11(7), 3561-3572. [Abstract]
  • Almazi, J., Mactier, S., Best, O., Crossett, B., Mulligan, S., Christopherson, R. (2012), Fludarabine nucleoside induces accumulations of p53, p63 and p73 in the nuclei of human B-lymphoid cell lines, with cytosolic and mitochondrial increases in p53. Proteomics - Clinical Applications. 6(5-6), 279-290. [Abstract]
  • Arakawa, K., Koh, J., Crossett, B., Torres, A., Kuchel, P. (2012), Detection of platypus-type L/D-peptide isomerase activity in aqueous extracts of papaya fruit. Biotechnology Letters. 34(9), 1659-1665. [Abstract]
  • Deutscher, A., Tacchi, J., Minion, F., Padula, M., Crossett, B., Bogema, D., Jenkins, C., Kuit, T., Walker, M., Djordjevic, S. (2012), Mycoplasma hyopneumoniae Surface proteins Mhp385 and Mhp384 bind host cilia and glycosaminoglycans and are endoproteolytically processed by proteases that recognize different cleavage motifs. Journal of Proteome Research. 11(3), 1924-1936. [Abstract]
  • Hare, N., Solis, N., Harmer, C., Marzook, N., Rose, B., Harbour, C., Crossett, B., Manos, J., Cordwell, S. (2012), Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain. BMC Microbiology. 12(1), 16. [Abstract]

2011

   
  • Henrich, S., Mactier, S., Best, G., Mulligan, S., Crossett, B., Christopherson, R. (2011), Fludarabine nucleoside modulates nuclear "survival and death" proteins in resistant chronic lymphocytic leukemia cells. Nucleosides, Nucleotides and Nucleic Acids. 30(12), 1181-1189. [Abstract]
  • Ly, L., Barnett, M., Zheng, Y., Gulati, T., Prineas, J., Crossett, B. (2011), Comprehensive Tissue Processing Strategy for Quantitative Proteomics of Formalin-fixed Multiple Sclerosis Lesions. Journal of proteome research. 10(10), 4855-4868. [Abstract]
  • Leahy, T., Marti, J., Crossett, B., Evan, G., Maxwell, W. (2011), Two-dimensional polyacrylamide gel electrophoresis of membrane proteins from flow cytometrically sorted ram sperm. Theriogenology. 75(5), 962-71. [Abstract]
  • Scott, N., Parker, B., Connolly, A., Paulech, J., Edwards, A., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S., Hojrup, P., Packer, N., Larsen, M., Cordwell, S. (2011), Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, HCD and ETD-MS applied to the N-linked glycoproteome of Campylobacter jejuni. Molecular & Cellular Proteomics. 10(2), M000031-MCP201. [Abstract]
  • Coleman, N., Yau, S., Wilson, N., Nolan, L., Migocki, M., Ly, M., Crossett, B., Holmes, A. (2011), Untangling the multiple monooxygenases of Mycobacterium chubuense strain NBB4, a versatile hydrocarbon degrader. Environmental Microbiology Reports. 3(3), 297-307.
  • Tokushige, N., Markham, R., Crossett, B., Ahn, S., Nelaturi, V., Khan, A., Fraser, I. (2011), Discovery of a novel biomarker in the urine in women with endometriosis. Fertility and Sterility. 95(1), 46-49. [Abstract]
  • Barker, E., Darby, A., Helps, C., Peters, I., Heesom, K., Arthur, C., Crossett, B., Hughes, M., Radford, A., Tasker, S. (2011), Molecular characterization of the uncultivatable hemotropic bacterium Mycoplasma haemofelis. Veterinary Research. 42(1), 83. [Abstract]

2010

   
  • Seymour, L., Deutscher, A., Jenkins, C., Kuit, T., Falconer, L., Minion, F., Crossett, B., Padula, M., Dixon, N., Djordjevic, S., Walker, M. (2010), A processed multidomain mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia. Journal of Biological Chemistry. 285(44), 33971-33978. [Abstract]
  • Schliep, M., Crossett, B., Willows, R., Chen, M. (2010), 18O Labeling of chlorophyll d in Acaryochloris marina reveal chlorophyll a and molecular oxygen are precursors. The Journal of biological chemistry. 285(37), 28450-6. [Abstract]
  • Koh, J., Chow, S., Crossett, B., Kuchel, P. (2010), Mammalian Peptide Isomerase: Platypus-Type Activity Is Present in Mouse Heart. Chemistry & biodiversity. 7(6), 1603-11. [Abstract]
  • Gandhi, K., McKay, F., Diefenbach, E., Crossett, B., Schibeci, S., Heard, R., Stewart, G., Booth, D., Arthur, J. (2010), Novel approaches to detect serum biomarkers for clinical response to interferon-beta treatment in multiple sclerosis. PLoS One. 5(5), e10484. [Abstract]
  • Zhou, L., Diefenbach, E., Crossett, B., Tran, S., Ng, T., Rizos, H., Rua, R., Wang, B., Kapur, A., Gandhi, K., Brew, B., Saksena, N. (2010), First evidence of overlaps between HIV-Associated Dementia (HAD) and non-viral neurodegenerative diseases: proteomic analysis of the frontal cortex from HIV+ patients with and without dementia. Molecular Neurodegeneration. 5, 27. [Abstract]
  • Scott, N., Marzook, N., Deutscher, A., Falconer, L., Crossett, B., Djordjevic, S., Cordwell, S. (2010), Mass spectrometric characterization of the Campylobacter jejuni adherence factor CadF reveals post-translational processing that removes immunogenicity while retaining fibronectin binding. Proteomics. 10(2), 277-288. [Abstract]

2008

   
  • Henrich, S., Gez, S., Crossett, B., Mulligan, S., Christopherson, R. (2008), Fludarabine Induces Differential Expression of Proteins in Human Leukemia and Lymphoma Cells. Nucleosides, Nucleotides & Nucleic Acids. 27(6), 634-640. [Abstract]
  • Bansal, P., Torres, A., Crossett, B., Wong, K., Koh, J., Geraghty, D., Vandenberg, J., Kuchel, P. (2008), Substrate specificity of platypus venom L-to-D-peptide isomerase. The Journal of biological chemistry. 283(0), 8969-75. [Abstract]
  • Cordwell, S., Len, A., Touma, R., Scott, N., Falconer, L., Jones, D., Connolly, A., Crossett, B., Djordjevic, S. (2008), Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies. Proteomics. 8(1), 122-139. [Abstract]
  • Cordwell, S., Crossett, B., White, M. (2008), Gel-Based Approaches. In: Proteomics. (pp.33-57).Germany: Wiley - VCH Verlag GmbH & Co. KGaA.
  • Crossett, B., Edwards, A., White, M., Cordwell, S. (2008), Statistical Analysis of Image Data Provided by Two-Dimensional Gel Electrophoresis for Discovery Proteomics. In: Methods in Molecular Medicine: Clinical Bioinformatics. (pp.271-285).New Jersey, USA: Humana Press.