Dr Mangala Nadkarni

C12 - United Dental Hospital
The University of Sydney

Telephone 61 2 9845-7826
Fax 61 2 9845 7599

Website Westmead Millennium Institute
University of Sydney
Google Scholar
Curriculum vitae Curriculum vitae

Biographical details

Dr Mangala Nadkarni obtained both BSc and MSc in Microbiology and PhD in Biochemistry from the University of Mumbai, India. Dr Nadkarni is a Senior Hospital Scientist with the Institute of Dental Research, Westmead Centre for Oral Health, Westmead Hospital and Senior Research Fellow with the Faculty of Dentistry, University of Sydney. She is a member of the Institutional Biosafety Committee, Western Sydney Local Health District and the American Society for Microbiology. Dr Nadkarni has held both international (NIH) and national (NHMRC) competitive project grants funding.

Research interests

Dr Nadkarni has research interests in molecular oral microbiology, in the area of dental caries and periodontal disease. Her research has defined the polymicrobial consortia associated with invasive infection of dentine and pulp using molecular techniques.

DENTAL CARIES AND INFECTION OF PULP

  • Quantification of total bacterial load using real time PCR

Determination of total bacterial load by real-time PCR using a universal probe & primers set, has remained amongst the 50 most-frequently read articles in Microbiology since 2003-2013 with 453 citations so far on ISI Web of Knowledge and is also classified amongst the 50 most-frequently read articles 2007-2013 in Microbiology highWire-hosted journals.

  • Phylogenetic analysis of molecular diversity occupying the niche of advanced caries using Sanger sequencing

Molecular cloning of 16S rRNA gene showed that the major bacterial consortia associated with advanced dentinal caries are from the families Lactobacillaceae, Streptococcaceae, Veillonellaceae (formerly Acidaminococcaceae), Eubacteriaceae, and Lachnospiraceae from the phylum Firmicutes; Coriobacteriaceae, Bifidobacteriaceae, and Propionibacteriaceae from the phylum Actinobacteria; and Prevotellaceae from the phylum Bacteroidetes, as well as Fusobacterium.

  • Spatial distribution of the microbiome responsible for progression of the infection from carious dentine into the pulp tissue using fluorescence in-situ hybridization (FISH)

Invasion of dental pulp is associated with a pronounced selection from the complex microbiota of the carious dentine. This work for the first time highlighted that lactobacilli are prominent in the initial stages of dental pulp infection.

· The new era of high-throughput next generation sequencing and oral infections

Completion of draft genomes of two tissue-invasive Lactobacillus rhamnosus strains implicated in the infection of dental pulpto better understand the genome composition and capabilities.

PERIODONTAL DISEASE

  • The virulence determinant, lysine gingipain (kgp) of Porphyromonas gingivalis

P. gingivalis could be categorized by differential PCR into four kgp biovars: HG66, 381, W83, and W83v, a domain variant of W83. Individual subjects show colonisation by a predominant biovar conserved in both subgingival and oral mucosal sites.

  • Pattern of distribution of Prevotella species/phylotypes and periodontal disease

Comprehensive analysis of Prevotella species/phylotypes associated with gingival sites, P. gingivalis load and its co-colonisation with other Prevotella species/phylotypes. Early colonization by some Prevotella species provide potential risk indicator for subsequent development of periodontal disease.

Current projects

  1. Characterisation of Lactobacillus rhamnosusstrains implicated in the infection of dental pulp.
  2. Age-dependent dysbiosis in human subgingival plaque microbiota and oral mucosal microbiota

Associations

Member of the American Society for Microbiology

Keywords

polymicrobial infection; dental caries; periodontal infection; FISH; Molecular biology

Selected grants

2009

  • Immune responses to biotype specific regions of the lysine gingipain (Kgp) of Porphyromonas gingivalis in patients with periodontitis; Nadkarni M, Hunter N; Australian Dental Research Foundation/Research Support.

2008

  • Microbial indicators for the conservative therapy of dentinal caries; Hunter N, Martin E, Nadkarni M, Swain M, Zoellner H, Jacques N; National Health and Medical Research Council (NHMRC)/Project Grants.

2006

  • Immunity to lys-gingipain domains in chronic periodontitis; Nadkarni M, Hunter N; Australian Dental Research Foundation/Project Support.

2003

  • Molecular epidemiology of infection in aboriginal communities; Hunter N, Jacques N, Nadkarni M, Martin E, Chapple C, Willcox M; National Health and Medical Research Council (NHMRC)/Strategic Research Development Committee (SDRC): Oral Health Research Grant.

Selected publications

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Journals

  • Nadkarni, M., Chen, Z., Wilkins, M., Hunter, N. (2014). Comparative Genome Analysis of Lactobacillus rhamnosus Clinical Isolates from Initial Stages of Dental Pulp Infection: Identification of a New Exopolysaccharide Cluster. PLoS One, 9(3), e90643. [More Information]
  • Chen, Z., Wilkins, M., Hunter, N., Nadkarni, M. (2013). Draft genome sequences of two clinical isolates of Lactobacillus rhamnosus initial stages of dental pulp infection. Genome Announcements, 1(1), e00073-12. [More Information]
  • Nadkarni, M., Browne, G., Chhour, K., Byun, R., Nguyen, K., Chapple, C., Jacques, N., Hunter, N. (2012). Pattern of distribution of Prevotella species/phylotypes associated with healthy gingiva and periodontal disease. European Journal of Clinical Microbiology and Infectious Diseases, 31(11), 2989-2999. [More Information]
  • Nadkarni, M., Simonian, M., Harty, D., Zoellner, H., Jacques, N., Hunter, N. (2010). Lactobacilli Are Prominent in the Initial Stages of Polymicrobial Infection of Dental Pulp. Journal of Clinical Microbiology, 48(5), 1732-1740. [More Information]
  • Li, N., Yun, P., Nadkarni, M., Nazila, B., Nguyen, K., Lee, M., Hunter, N., Collyer, C. (2010). Structure determination and analysis of a haemolytic gingipain adhesin domain from Porphyromonas gingivalis. Molecular Microbiology, 76(4), 861-873. [More Information]
  • Ye, P., Nadkarni, M., Simonian, M., Hunter, N. (2009). CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biology, 10, 1-16. [More Information]
  • Nadkarni, M., Chhour, K., Browne, G., Jacques, N., Hunter, N. (2009). Lysine gingipain (kgp) biovars of Porphyromonas gingivalis exhibit differential distribution on oral mucosal sites. Journal of Clinical Microbiology, 47(10), 3350-3352. [More Information]
  • Nadkarni, M., Martin, E., Hunter, N., Jacques, N. (2009). Methods for optimizing DNA extraction before quantifying oral bacterial numbers by real-time PCR. FEMS Microbiology Letters, 296, 45-51. [More Information]
  • Smith-Vaughan, H., Byun, R., Halpin, S., Nadkarni, M., Jacques, N., Hunter, N., Morris, P., Leach, A. (2008). Interventions for prevention of otitis media may be most effective if implemented in the first weeks of life. International Journal of Pediatric Otorhinolaryngology, 72, 57-61. [More Information]
  • Smith-Vaughan, H., Byun, R., Nadkarni, M., Jacques, N., Hunter, N., Halpin, S., Morris, P., Leach, A. (2006). Measuring nasal bacterial load and its association with otitis media. B M C Ear Nose and Throat Disorders, 6(1), 10. [More Information]
  • Ye, P., Nadkarni, M., Hunter, N. (2006). Regulation of E-cadherin and TGF-β3 expression by CD24 in cultured oral epithelial cells. Biochemical and Biophysical Research Communications, 349(1), 229-235. [More Information]
  • Ye, P., Simonian, M., Nadkarni, M., DeCarlo, A., Chapple, C., Hunter, N. (2005). Identification of epithelial auto-antigens associated with periodontal disease. Clinical and Experimental Immunology, 139(2), 328-337. [More Information]
  • Chhour, K., Nadkarni, M., Byun, R., Martin, E., Jacques, N., Hunter, N. (2005). Molecular Analysis of Microbial Diversity in Advanced Caries. Journal of Clinical Microbiology, 43(2), 843-849. [More Information]
  • Nadkarni, M., Byun, R., Chhour, K. (2005). Molecular taxonomy of polymicrobial diseases -finding novel bacteria not previously considered to be associated with oral diseases. Microbiology Australia, 26(3), 117-119.
  • Nadkarni, M., Caldon, E., Chhour, K., Fisher, I., Martin, E., Jacques, N., Hunter, N. (2004). Carious Dentine Provides a Habitat for a Complex Array of Novel Prevotella-Like Organisms. Journal of Clinical Microbiology, 42(11), 5238-5244.
  • Nadkarni, M., Nguyen, K., Chapple, C., DeCarlo, A., Jacques, N., Hunter, N. (2004). Distribution Of Porphyromonas Gingivalis Biotypes Defined By Alleles Of The Kgp (Lys-Gingipain) Gene. Journal of Clinical Microbiology, 42(8), 3873-3876.
  • Byun, R., Nadkarni, M., Chhour, K., Martin, E., Jacques, N., Hunter, N. (2004). Quantitative Analysis Of Diverse Lactobacillus Species Present In Advanced Dental Caries. Journal of Clinical Microbiology, 42(7), 3128-3136.
  • DeCarlo, A., Nadkarni, M., Paramaesvaran, M., Yun, P., Collyer, C., Hunter, N. (2004). Serum Antibodies Against The Hemoglobin-Binding Domain (HA2) Of Porphyromonas Gingivalis. Journal of Periodontal Research, 39(4), 228-235.
  • Ye, P., Harty, D., Chapple, C., Nadkarni, M., DeCarlo, A., Hunter, N. (2003). Streptococci and Actinomyces induce antibodies which cross react with epithelial antigens in periodontitis. Clinical and Experimental Immunology, 131(3), 468-476.
  • Nadkarni, M., Martin, E., Jacques, N., Hunter, N. (2002). Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology, 148(1), 257-266.
  • Martin, E., Nadkarni, M., Jacques, N., Hunter, N. (2002). Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. Journal of Clinical Microbiology, 40(5), 1698-1704.

Patents

  • Martin, E., Hunter, N., Jacques, N., Nadkarni, M. (2007). A Method of Detecting Microorganisms. Patent No. 1311706, 2001276180, 7244480. Austria, Belgium, Cyprus, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Monaco, Netherlands, Portugal, Spain, Sweden, Switzerland, Turkey, United Kingdom.

Reference Works

  • Nadkarni, M., Martin, E., Jacques, N., Hunter, N. (2009). Caries. Encyclopedia of Molecular Mechanisms of Disease. (Vol. 1, pp. 279-280). Berlin: Springer.

2014

  • Nadkarni, M., Chen, Z., Wilkins, M., Hunter, N. (2014). Comparative Genome Analysis of Lactobacillus rhamnosus Clinical Isolates from Initial Stages of Dental Pulp Infection: Identification of a New Exopolysaccharide Cluster. PLoS One, 9(3), e90643. [More Information]

2013

  • Chen, Z., Wilkins, M., Hunter, N., Nadkarni, M. (2013). Draft genome sequences of two clinical isolates of Lactobacillus rhamnosus initial stages of dental pulp infection. Genome Announcements, 1(1), e00073-12. [More Information]

2012

  • Nadkarni, M., Browne, G., Chhour, K., Byun, R., Nguyen, K., Chapple, C., Jacques, N., Hunter, N. (2012). Pattern of distribution of Prevotella species/phylotypes associated with healthy gingiva and periodontal disease. European Journal of Clinical Microbiology and Infectious Diseases, 31(11), 2989-2999. [More Information]

2010

  • Nadkarni, M., Simonian, M., Harty, D., Zoellner, H., Jacques, N., Hunter, N. (2010). Lactobacilli Are Prominent in the Initial Stages of Polymicrobial Infection of Dental Pulp. Journal of Clinical Microbiology, 48(5), 1732-1740. [More Information]
  • Li, N., Yun, P., Nadkarni, M., Nazila, B., Nguyen, K., Lee, M., Hunter, N., Collyer, C. (2010). Structure determination and analysis of a haemolytic gingipain adhesin domain from Porphyromonas gingivalis. Molecular Microbiology, 76(4), 861-873. [More Information]

2009

  • Nadkarni, M., Martin, E., Jacques, N., Hunter, N. (2009). Caries. Encyclopedia of Molecular Mechanisms of Disease. (Vol. 1, pp. 279-280). Berlin: Springer.
  • Ye, P., Nadkarni, M., Simonian, M., Hunter, N. (2009). CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biology, 10, 1-16. [More Information]
  • Nadkarni, M., Chhour, K., Browne, G., Jacques, N., Hunter, N. (2009). Lysine gingipain (kgp) biovars of Porphyromonas gingivalis exhibit differential distribution on oral mucosal sites. Journal of Clinical Microbiology, 47(10), 3350-3352. [More Information]
  • Nadkarni, M., Martin, E., Hunter, N., Jacques, N. (2009). Methods for optimizing DNA extraction before quantifying oral bacterial numbers by real-time PCR. FEMS Microbiology Letters, 296, 45-51. [More Information]

2008

  • Smith-Vaughan, H., Byun, R., Halpin, S., Nadkarni, M., Jacques, N., Hunter, N., Morris, P., Leach, A. (2008). Interventions for prevention of otitis media may be most effective if implemented in the first weeks of life. International Journal of Pediatric Otorhinolaryngology, 72, 57-61. [More Information]

2007

  • Martin, E., Hunter, N., Jacques, N., Nadkarni, M. (2007). A Method of Detecting Microorganisms. Patent No. 1311706, 2001276180, 7244480. Austria, Belgium, Cyprus, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Monaco, Netherlands, Portugal, Spain, Sweden, Switzerland, Turkey, United Kingdom.

2006

  • Smith-Vaughan, H., Byun, R., Nadkarni, M., Jacques, N., Hunter, N., Halpin, S., Morris, P., Leach, A. (2006). Measuring nasal bacterial load and its association with otitis media. B M C Ear Nose and Throat Disorders, 6(1), 10. [More Information]
  • Ye, P., Nadkarni, M., Hunter, N. (2006). Regulation of E-cadherin and TGF-β3 expression by CD24 in cultured oral epithelial cells. Biochemical and Biophysical Research Communications, 349(1), 229-235. [More Information]

2005

  • Ye, P., Simonian, M., Nadkarni, M., DeCarlo, A., Chapple, C., Hunter, N. (2005). Identification of epithelial auto-antigens associated with periodontal disease. Clinical and Experimental Immunology, 139(2), 328-337. [More Information]
  • Chhour, K., Nadkarni, M., Byun, R., Martin, E., Jacques, N., Hunter, N. (2005). Molecular Analysis of Microbial Diversity in Advanced Caries. Journal of Clinical Microbiology, 43(2), 843-849. [More Information]
  • Nadkarni, M., Byun, R., Chhour, K. (2005). Molecular taxonomy of polymicrobial diseases -finding novel bacteria not previously considered to be associated with oral diseases. Microbiology Australia, 26(3), 117-119.

2004

  • Nadkarni, M., Caldon, E., Chhour, K., Fisher, I., Martin, E., Jacques, N., Hunter, N. (2004). Carious Dentine Provides a Habitat for a Complex Array of Novel Prevotella-Like Organisms. Journal of Clinical Microbiology, 42(11), 5238-5244.
  • Nadkarni, M., Nguyen, K., Chapple, C., DeCarlo, A., Jacques, N., Hunter, N. (2004). Distribution Of Porphyromonas Gingivalis Biotypes Defined By Alleles Of The Kgp (Lys-Gingipain) Gene. Journal of Clinical Microbiology, 42(8), 3873-3876.
  • Byun, R., Nadkarni, M., Chhour, K., Martin, E., Jacques, N., Hunter, N. (2004). Quantitative Analysis Of Diverse Lactobacillus Species Present In Advanced Dental Caries. Journal of Clinical Microbiology, 42(7), 3128-3136.
  • DeCarlo, A., Nadkarni, M., Paramaesvaran, M., Yun, P., Collyer, C., Hunter, N. (2004). Serum Antibodies Against The Hemoglobin-Binding Domain (HA2) Of Porphyromonas Gingivalis. Journal of Periodontal Research, 39(4), 228-235.

2003

  • Ye, P., Harty, D., Chapple, C., Nadkarni, M., DeCarlo, A., Hunter, N. (2003). Streptococci and Actinomyces induce antibodies which cross react with epithelial antigens in periodontitis. Clinical and Experimental Immunology, 131(3), 468-476.

2002

  • Nadkarni, M., Martin, E., Jacques, N., Hunter, N. (2002). Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology, 148(1), 257-266.
  • Martin, E., Nadkarni, M., Jacques, N., Hunter, N. (2002). Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. Journal of Clinical Microbiology, 40(5), 1698-1704.

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