Live Cell Imaging Facility

The facililty

The system comprises:

• Olympus IX70 inverted microscope
• Fluorescence System
• MetaFluor and MetaMorph software for fluorescence image acquisition.
• Hamamatsu multi-format cooled CCD camera
• TMC Isolation table

Olympus IX70 Inverted Light Microscope

Location

Room N135, Anderson Stuart Buidling
Olympus IX70 inverted light microscope

Configured for transmission light (bright field, DIC, simple polarized light) and reflected light observation.

Illumination sources

• 12V/ 100W Halogen lamp
• HBO 103W/2 Hg short arc lamp
• 175W Ozone-free Xenon lamp

Objectives

• UPlanApo 10x/ NA 0.4/ WD 3.1mm
• UApo 20x/ NA 0.75/ WD 0.55mm
• UApo 40x water corr/ NA 1.15/ WD 0.26mm/ coverglass 0.13-0.25
• UPlanApo 60x water corr/ NA 1.20/ WD 0.25mm/ coverglass 0.13-0.21

Filters

• Endow GFP Bandpass Emission filter (Chroma filter 41017: HQ470/40, Q495LP, HQ525/50)

Fluorescence system

The Sutter DG-4 Wavelength Switcher is a combined Xenon Arc lamp and high speed wavelength switcher, ideal for fast ion imaging where image ratios at different excitation wavelengths are required at high frame rates. It comprises:

• Built-in 175W ozone-free Xenon lamp
• Uses up to 4 standard (25mm diameter) interference filters
• Beam is switched between filters in less than 1.2 ms
• Allows video-rate ratio imaging (depending on camera hardware)
• Liquid light guide provides vibration isolation from microscope
• Integral shuttering and neutral density filtering

Filters
The system is currently configured for ratio imaging of:

• FURA-2 (Omega filter XF04: Exc1 340AF15, Exc2 380AF15, DM 415 DCLP, Em 510WB40).
• Di-8-ANEPPS (Omega filter XF17: Exc1 490DF20, Exc2 440AF21, DM 550DCLP, Em 660DF50)

Software

• Fluorescence image acquisition is controlled by MetaFluor (Universal Imaging)
• Image processing is achieved with MetaMorph (Universal Imaging)

File saving

• Floppy 3.5" 1.44MB (confocal data)
• DVD and CD burning (fluorescence data)