Advanced Microscopy Facility Micrograph of the Year Competition
One of the highlights of every year at the Advanced Microscopy Facility is the Micrograph of the Year Competition. This competition is open to all Bosch members and registered users of the Facility.
Micrographs of 2011
The Bosch Institute Advanced Microscopy Facility is now accepting entries for the 2011 Micrograph of the Year Competition.
(i) Choose your BEST micrograph(s) taken with a transmitted light, fluorescence, confocal or electron microscope. No more than THREE images can be submitted per person.
(ii) Print high resolution image(s) on good quality photo paper. Minimum image size: A4 or 210 x 297 mm.
(iii) Complete an official entry form.
(iv) Deliver entries to Dr Louise Cole, Rm S452, Anderson Stuart Building F13, The University of Sydney.
All entries must be received by 5.00 pm AEST on Thursday, November 24th 2011. No entries will be accepted after this time. Only University of Sydney, Bosch Institute and Bosch AMF registered members can apply.
Selected entries will be displayed in the Anderson Stuart Common from November 28th 2011 for one week only and the top winning entries will be announced at the end of the Bosch Young Investigators Symposium at the University of Sydney on Thursday December 1st.
The fabulous prizes include a Notebook PC, digital camera, binoculars, $200 iTunes gift voucher, $250 prize money, a lab lunch for 6 and a backpack gift pack! Prizes kindly donated by the competition sponsors: BioStrategy/Molecular Devices, Nikon/Coherent Scientific, Carl Zeiss, LasTek/LaVision BioTec, Bosch Institute, Perkin Elmer and Leica Microsystems respectively. We are extremely grateful to all of the Competition sponsors for their support for this competition and the Bosch AMF.
For further information relating to this competition and the Bosch AMF, please contact Dr Louse Cole by email: .
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Micrographs of 2010
It has been yet another great year for the Bosch Institute Advanced Microscopy Facility Micrograph Competition. In the end there were SEVENTY-TWO entries! So a huge thank-you to all who entered. Assoc. Prof Cedric Shorey (Honorary Associate in the Discipline of Anatomy and Histology) and Jenny Whiting (EMU, previously Wellcome Trust Foundation, UK) had the difficult task (once again) of judging the competition ie. selecting the Top 20 as well as the winners. This job gets tougher every year!
As usual, the TOP 20 micrographs were on display in the Anderson Stuart Common Room from Monday November 22nd to 4.00 pm Friday December 3rd inclusive.
Bosch Institute Advanced Microscopy Facility Top 20 Micrographs 2010
Images taken by Bosch members and AMF users:
Top row (left to right): Carsten Minten, Jarred Bambridge, Michelle Gerke, Yui Kaneko, Haydn Allbutt
Second row (left to right): Hyunchul Lee, Nathan Coorey, Yui Murata & Marco Morsch, Megan Steain, Steven Eamegdool
Third row (left to right): Tariq Chohan, Gagandeep Kaur, Sharissa Latham, Louise Baxter, Steven Eamegdool
Bottom row (left to right): Nick Cole, Hussein Mansour, Andrew Arthur, Michael Lovelace, Yan Li
This year there were seven winners (this includes the most-coveted Micrograph of the Year award). All winners were announced after the Bosch Young Investigators Symposium on Tuesday November 30th.
First prize: $250 sponsored by Bosch Institute
Yui Murata and Marco Morsch
Bill Phillips lab, Physiology, Bosch Institute
3D projected image of three neuromuscular junctions
Second prize: iPod Touch sponsored by LasTek
Michael Lovelace
Tailoi Chan-Ling lab, Discipline of Anatomy and Histology
“The dawn of neurodevelopment – the migratory journey of neural precursors”
Third prize: Compact binoculars sponsored by Carl Zeiss
Nathan Coorey
Gillies Research Group, Clinical Ophthalmology and Eye Health, Save Sight Institute
Organisation of Müller cells in the mouse retina.
Fourth prize: Compact digital camera sponsored by Olympus Australia
Andrew Arthur
Karen Cullen Laboratory, Discipline of Anatomy & Histology
Image showing clear co-localisation of Alzheimer plaques and microvessels in individuals with Alzheimer’s disease.
Fifth Prize: Compact digital camera sponsored by Nikon/Coherent Scientific
Gagandeep Kaur
Michelle Gerke lab, Discipline of Anatomy & Histology
The sick and the dead....image of primary sensory neurons
Sixth prize: iPod shuffle sponsored by Perkin Elmer
Yui Kaneko
Chris Murphy Lab, Anatomy & Histology, Bosch Institute
“Landing Blastocyst: The moment of implantation”
Seventh prize: iPod nano sponsored by Leica Microsystems Australia
Haydn Allbutt
Physiology, School of Medical Sciences,
A histochemically-stained coronal section through the thalamus of a Sprague dawley rat
This competition was proudly sponsored by Carl Zeiss, Olympus, Leica Microsystems, Nikon Australia, Perkin Elmer, LasTek and the Bosch Institute.
Micrographs of 2009
Thanks to all that entered the Bosch Institute AMF Micrograph of the Year Competition in 2009 (and to those supervisors who encouraged their students!). It was another great year. The Top 20 images were on display in the Anderson Stuart Common Room and the Top 5 winners were announced at the end of the Bosch Young Investigators Symposium 2009. Thanks again to the two judges, Assoc Prof. Cedric Shorey (Anatomy & Histology) and Jenny Whiting (EMU), for doing such a fine but difficult job in selecting the winners. I would like to say a special thanks to all the sponsors, since this Competition would have not been possible without their very kind donation of the prizes! Special thanks to Carl Zeiss, Olympus Australia, Nikon/Coherent Scientific, Leica Microsystems and the Bosch Institute.
Bosch Institute Advanced Microscopy Facility Top 20 Micrographs 2009
Images taken by Bosch members and AMF users (left to right):
Top row: Jin Huang, Michael Lovelace, Lisa Nivison-Smith, Jenny Brockhausen, Andrew Affleck
Second row: Luis Munoz-Erazo, Komal Prabhu, Emily Don, Connie Poon, Francine Marquez
Third row: Hussein Mansour, Lisa Nivison-Smith, Hussein Mansour, Sivaraman Purushothuman, De Lian Goh
Bottom row: Ineka Whiteman, Sivaraman Purushothuman, Emily Don, Jenny Brockhausen, Nazanin Ghazanfari
Congratulations to the Top 5 2009 winners shown here from left to right:
First prize: Connie Poon (Anatomy & Histology, Bosch Institute)
3D projected image of a rat blastocyst in the process of hatching from its external zona pellucida (patchy outer coat surrounding the cellular mass of the blastocyst). Image taken using Zeiss LSM 510 Meta confocal microscope. Blue staining shows cell nuclei of the trophoblast cells comprising the blastocyst and green staining shows the localisation of Mucin 15 protein. This hatching process is necessary to allow the blastocyst to adhere and interact with the epithelial cells of the uterus to allow for implantation and subsequent pregnancy to occur.
Second prize: Hussein Mansour (Anatomy & Histology, Bosch Institute)
Immunohistochemistry of retinal whole mounts shows α-smooth muscle actin (SMA) labelling of inter-laminar fibrous layer (red), NG2-labelling of pericyte cells (blue) and nestin-labelled intermediate filamentous protein at leading edge and branch points (green). This image demonstrates the multi-cytoarchitectural composition of small capillaries that ensures vessel growth and stability of newly formed and aging blood vessels.
Third prize: Emily Don (Anatomy & Histology, Bosch Institute)
Confocal image showing the ventral view of a juvenile Zebrafish (Danio rerio). Anterior is to the top of the image. The newly formed pelvic fins can be seen just anterior to the cloaca, either side of the retreating larval fin fold.
Fourth prize: Andrew Affleck (Physiology, Bosch Institute)
Maximum intensity projection of a 3D orthogonal image of chicken basilar papilla hair cells stained with anti-CtBP2 (C-terminal binding protein) (green) for labeling of ribbon synapse and anti-GluR 2/3 (Glutamate receptor) (red) for labeling glutamate receptor types 2 and 3 inherent in the afferent nerve terminals on the post-synaptic surface. The latter receptor unspecifically aggregated to supporting structures most notably the hair bundle on the apical border to the hair cell. Image taken on Zeiss LSM 510 Meta confocal microscope.
Fifth prize: Sivaraman Purushothuman (Physiology, Bosch Institute)
Structure of cell death: In the brain of a transgenic mouse, haemorrhage has caused cell death and cellular debris is compacted by activated macrophages. The compacting is non-random; an autofluorescent core of yellow-green material is surrounded by a halo of fragmented nuclear DNA (blue), with an outer halo of cytoskeletal fragments of dead astrocytes (red). Image taken with deconvolution microscope.
Micrographs of 2008
In 2008, the competition was judged by Assoc. Prof. Cedric Shorey. Assoc. Prof. Shorey was astounded by the high standard of images submitted for the competition. Almost 60 images were received illustrating a wide variety of biological samples, cell and tissue preparation methods and both light and laser microscope techniques.
The Top 20 micrographs were mounted and displayed in the Anderson Stuart Common Room during the week of the Bosch Young Investigators (BYI) Symposium in December 2008.
Bosch Institute Advanced Microscopy Facility Top 20 Micrographs 2008
Images taken by Bosch members and AMF users (left to right):
Top row: Richard Sarafian, Richard Sarafian, Caroline Reddel, Daniel Bax, Minh Huynh
Second row: Komal Prabhu, Andrea Markus, Liz Sloan, Komal Prabhu, Megan Steain
Third row: Michael Lovelace, Yan Li, Hyunchul Lee, Rachel Terry, Yui Kaneko
Bottom row: Evan McFarland, Nick Whitehead, Boating Zhang, Erica Jeong, Margot Day
At the conclusion of the BYI Symposium, the Top 5 winners (images shown below) were presented with fabulous prizes that were generously donated by the Bosch Institute, Carl Zeiss, Nikon and Coherent Scientific, Olympus Australia and Perkin Elmer. Well done to all who entered the Competition!
Top 5 Micrographs 2008
From left to right:
First prize: Yan Li (Human Reproduction Unit, Royal North Shore Hospital)
Fertilization Point: Mouse 1-cell embryo double-stained for nuclear DNA (Propidium Iodide, red) and methylated 5-cytidine in DNA (FITC. green). It shows the time of fertilization when a sperm first attaches to the surface of the oocyte. This triggers the second meiotic division. It also shows differential DNA methylation in each set of chromosomes.
Second prizes:
- Nick Whitehead (Physiology, Bosch Institute)
Image shows one of the NADPH oxidase proteins, p22phox (green), which co-localises with Caveolin-3 (red) along the muscle fibre surface membrane of an mdx mouse. Nuclei are identified by DAPI staining (blue). - Andrea Markus (Basic & Clinical Genomics Lab, Physiology, Bosch Institute) Image shows co-localization of the RNA binding protein RBM4 (red) with gamma-tubulin (green) in the centrosomes of HEK293 cells during mitosis. Cell nuclei are stained with DAPI (blue).
- Erica Jeong (Anatomy, Bosch Institute) Image shows a 4-day old in vitro cultured cell from tectal tissues of 7-day old chick embryos. Cells were stained with total Tau antibody (red), phalloidin (green) and DAPI (blue).
Third prize: Hyunchul Lee (Physiology)
A medium-spiny projection neuron in the mouse caudate-putamen was filled with neurobiotin using a patch-pipette and visualized using cobalt chloride and diaminobenzidine. These cells reach out with their multiple spiny arbors to gather valuable information arriving from the cortex, sending their own message via long axons to the globus pallidus (pale globe) and the dopamine-secreting subtantia nigra (black substance).
Bosch AMF Micrograph Competition's Top 20 Images
From left to right: Andrea Markus checking out the top 20 images on display!, 2008 winner Nick Whitehead (middle) receives his prize from Assoc. Prof Cedric Shorey and Sue Hart (Carl Zeiss), 2008 winner Erica Jeong (middle) receives her prize from Assoc. Prof. Cedric Shorey and Sophie Tran (Nikon/Coherent Scientific).