Flow cytometry laboratory
This facility has been developed recently as a result of some major grant success by Professor Nicholas King, supported by other Bosch Institute members, and researchers from elsewhere in Sydney. The facility currently occupies temporary accommodation but it is planned to establish a central core facility, perhaps one that is shared with another major research institute within the Central Research Hub based around the University of Sydney and Royal Prince Alfred Hospital.
The facility contains the following cutting edge equipment for flow cytometry:
Please contact the Flow Cytometry Officer if you wish to be trained in the use of a piece of equipment and to become a registered user.
Amnis ImageStream 100
To our knowledge, this is the only such piece of equipment within Australia. It combines the best attributes of a conventional flow cytometer with those of a fluorescence microscope. It has the abilities of a conventional analytical flow cytometer but can also capture and process 6 different high resolution digital images of each cell. This allows Bosch Institute researchers to study events going on inside the cell in a dynamic and detailed way. This is an enormously powerful and flexible technology that can be applied to studies right across the range of research programs going on in the Bosch Institute.
Becton Dickinson FACSAria
This "high end" flow cytometer was acquired in 2005. There are only a handful of its type in Australia. It has excellent analytical and cell sorting capabilities.
FACScalibur Sort
This is a standard flow cytometer with analytical and sorting capabilities. It is used for routine applications, freeing the more advanced ImageStream 100 and FACS Aria for applications that require more sophisticated technology.
Sir Zelman Cowen Flow Cytometry Officer
Position vacant at present - a new officer will be in post in the new year.