Assays / Applications

The BMSF caters for a range of analytical equipment including HPLC, UPLC coupled with various detectors such as Diode Array Detection, Florescence and Electrochemical detectors, Single quadruple and Triple quadruple mass spectrometers (each coupled to UV-Vis detectors). Both UV peak based and mass based semi-prep fraction collections are also available.

Assays are currently available at BMSF are as follows (not limited to the list below):

o Cl-Tyrosine analysis in cells and tissues (LC-MS/MS);

o Apolipoproteins and Serum amyloid A (SAA) analysis (LC-UV);

o Adenosine analysis in cells (LC-UV; LC-MS/MS);

o Synthesized antifibrotic drugs Px25 and Px64 in plasma (LC-MS/MS);

o MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and its metabolite MPP+ (LC-MS/MS);

o Arachidonic acid analysis in cells (LC-MS/MS);

o Risperidone analysis in human tissues (LC-MS/MS);

o Platelet-activating factor (PAF) analysis in cells (LC-MS/MS);

o Glutamin analysis in cells (LC-UV);

o Redox-active compounds analysis in Geothrix fermentans (HPLC-EC);

o F2-sioprostane -a wide-used lipid marker in biological system using HPLC-QQQ-MS;

o Cocktails of kinetic study of 11 drugs (CYP450 and their metabolites) in human plasma using HPLC-MS/MS;

o Quantitative analysis for an anti-cancer drug actinomycin D (LC-MS/MS);

o Phosphatidylcholine, lysophosphatidylcholine and palmitic acid and related metabolites (LC-MS/MS);

o Fatty acids including phytoene, phytofluene, neurosporene, lycopene, gamma-carotene, beta-carotene (HPLC-UV, HPLC-SQ-MS and HPLC-QQQ-MS);

o Drugs with potential inhibition ability to efflux pumps of mycobacterium tuberculosis including Rifapentine, 25-desacetyl rifampicin, Thioridazine hydrochloride, Verapamil hydrochloride, Norverapamil hydrochloride, Rifampicin (LC-MS/MS);

o Capsaicin analysis (LC-MS/MS);

o Analysis of prednisolone -a corticosteroid drug using HPLC-MS/MS;

o A LC-MS/MS method to quantify phosphatidylcholine, lysophosphatidylcholine, oleic acid and related metabolites;

o LC-MS/MS analysis for perindopril and its metabolite perindoprilat in human plasma;

o Detection of labetalol– a drug treating high blood pressure(LC-MS/MS);

o A cocktail pharmacokinetic study for 10 drugs and their respective 10 metabolites to investigate geriatric outcomes (LC-MS/MS);

o Detection of dehydrocorticosterone -a corticosteroid drug using HPLC-QQQ-MS;

o Analysis of sulforaphane and phenacetin in human plasma (LC-MS/MS);

o A complete LC-M/MS method for analysis of Vitamin D2 and D3 and their respective metabolites;

o A very sensitive LC-MS/MS method to detect proline using deuterated proline as internal std in embryo and plasma samples (School of Physiology);

o A sensitive LC-MS/MS method to quantify levels of Phosphatidylethanol and 1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphoethanol (PLPEth) in human brain tissue post mortem;

o A sensitive LC-MS/MS method to analyse levels of salicylic acid in human and mice plasma to investigate kinetics of aspirins in human and mice serum;

o A LC-MS/MS method developed to measure 3-MCPD, a carcinogenic compound and its four metabolites in variety of food;

o A LC-MS/MS method to measure levels of Strearoyl CoA and Oleoyl CoA in various kinds of cell lines;

o A LC-MS/MS method to detect levels of thyroid hormones including T2, T3 and T4 in fish tissues;

o A cocktails assay to monitor 9 compounds and their metabolites in human plasma from subjects with Osteoarthritis for a pharmacokinetics study (Royal North Shore Hospital);

o LC-MS/MS quantification of Testosterone and Dihyrotestosterone in mouse plasma;

o Serine and Glycine and the 4 carbon labelled amino acids in human retina;

o Quantifying Cortisol and cortisone in mouse plasma using LC-MS/MS;

o Quantifying Lipoxin A4 and its epimer aspirin-triggered 15-epi-lipoxin A4 in human plasma;

o LC-MS/MS analysis for Resveratrol, Rapamycin and Metformin and their respective metabolites;

o Oxytocin and its 3 metabolites in tissue samples.

If you would like to set up new assays / applications at BMSF, please contact BMSF Officer. Phone: 02 9036 3460 / M: 0423 335 188; Email: