LIVE CELL ANALYSIS
Live Cell Imaging
- IncuCyte ZOOM live cell imaging systems (Room N136 and Room N232, Anderson Stuart Building, Room 616, Molecular Bioscience Building G08) (funded by Sydney Cancer Research Fund, Cancer Institute of NSW and USYD Collaborative Research Equipment grant) (User Manual) (IncuCyte ZOOM software version 2016A download) - online booking
IncuCyte ZOOM
- Nanolive 3D Cell Explorer with IBIDI Gas Incubation system for CO2 and O2 and Nanolive-ibidi Heating System (Room N232, Anderson Stuart Building) (Nanolive User Manual) (Ibidi Gas Incubation System Instruction Manual) (Nanolive-ibidi Heating System Instruction Manual) (IncuControl Software Instruction Manual) (Sample Preparation Protocols) (Rules of Thumb) (Case Studies) - Online Booking
Nanolive 3D explorer and Ibidi Heating and Gas incubation Systems
- HoloMonitor M4 system (funded by NHMRC) (Room 616, Molecular Bioscience Building G08) - Online Booking
HoloMonitor M4
- Cytation 3 Cell Imaging Multi-mode Reader (funded by Cancer Institute of NSW) (Room 616, Molecular Bioscience Building G08) (Cytation 3 Operator Manual) (Automated Digital Microscopy Application Guide) - online booking
Cytation 3
- Olympus CKX31/CKX41 Phase Contrast and Fluorescence Inverted Microscope with ProgRes C5 Cooled Colour CCD Camera (N135, Anderson Stuart PC2 Facility) (Olympus CKX31/CKX41 Instructions) (Reflected Fluorescence System Instructions) (ProRes MAC CapturePro 2.7 Manual) - online booking
xCelligence Real Time Cell Analysis Systems
xCelligence Real Time Cell Analysis (RTCA) systems employ noninvasive impedance microelectrodes in automated high-throughput microplate formats for label-free, real-time monitoring of cell proliferation, cell size/morphology, cell-substrate attachment quality, and cell invasion/migration. Our facility has a xCelligence RTCA SP (single 96-well plate) system and a xCelligence RTCA DP (dual purpose) system.
- xCelligence RTCA SP system (Room N137, Anderson Stuart PC2 facility) (funded by the Clive & Vera Ramaciotti Foundations and Prostate Cancer Foundation of Australia) (RTCA 1.2 Software Manual) (SP system Operator Manual) - online booking
- xCelligence RTCA DP system (Room N137, Anderson Stuart PC2) (funded by the Clive & Vera Ramaciotti Foundations and Prostate Cancer Foundation of Australia) (RTCA 1.2 Software Manual) (DP system Operator Manual) - online booking
xCelligence RTCA SP
xCelligence RTCA DP
Cell Energy Metabolic Analysis
Seahorse XF Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate to interrogate key cellular functions such as mitochondrial respiration and glycolysis. The instruments perform compound addition and mixing, label-free detection, and automatic calculation of OCR and ECAR in real time. Click here to see the publications using the Seahorse XF Analyzers. You can search the list of Seahorse XF publications by research area, cell type, cell line, XF assay or author via the link.
- Seahorse XF24-3 Analyzer (Room N136, Anderson Stuart PC2 facility) (funded by Cancer Institute of NSW) (XF24 manual) (XF24 software v1.8.1 download) - online booking
- Seahorse XF96 Analyzer (Room N136, Anderson Stuart PC2 facility) (funded by Cancer Institute of NSW) (XF96 manual) (XF96 software v1.4.2 download) - online booking
- Seahorse Prep Station (Room N136, Anderson Stuart PC2 facility) (funded by Cancer Institute of NSW) - online booking
Seahorse XF24-3 Analyzer
Seahorse XF96 Analyzer
Seahorse Prep Station
Flow Cytometers
- Muse Cell Analyzer (Room N138, Anderson Stuart PC2 Facility) (Muse User Guide) (Muse System Quick Start Cards) (Muse Cell Analyzer software Download) - online booking
- Guava easyCyte 6-2L Benchtop Flow Cytometer (Room N138, Anderson Stuart PC2 Facility) (Guava easyCyte System User Guide) (GuavaSoft Modules User Guide) - online booking
Muse Cell Analyzer
Guava easyCyte 6-2L
Hypoxic Chambers
- Don Whitley H135 Hypoxic Workstation (funded by NHMRC Equipment Grant) (Room N232, Anderson Stuart) (H135 User Manual) - online booking
- Don Whitley i2 Hypoxic Workstation (funded by NHMRC Equipment Grant) (Room N232, Anderson Stuart) (i2 User Manual) - online booking
Don Whitley H135
Don Whitley i2
LumiCycler
LumiCycle performs high-throughput luminometry on self-luminous tissues, such as those from transgenic animals containing the luciferase gene. The system is equipped with 4 photon-counting photomultiplier tubes, each selected for low dark counts and high sensitivity in the green portion of the spectrum at which luciferase emits light. 32 tissue samples, each in a 35 mm Petri dish, can be counted at one time. 8 dishes share each photodetector using a turntable device that alternately brings each dish under the detector, one at a time.
- Lumicycle 32 system (Room 613, Molecular Bioscience Building G08) Online booking
Lumicycle 32
Mechanical Cell Strain Instrument
- STREX Mechanical Cell Strain Instrument ST-140-04 (unidirectional stretch, multiple chambers) (Room 616, Molecular Bioscience Building G08) (User Manual) online booking
STREX Mechanical Cell Strain Instrument (unidirectional stretch, multiple chambers) is an automated instrument designed to hold up to 6 strain chambers with precise uniaxial mechanical strain. The robust and easy to use design ensures that cells grow over long periods of time with regular cyclic stretching or compression. This unit allows you to control the frequency and degree of the strain to produce 64 unique strain settings. The instrument fits in a standard cell culture incubator.
STREX Mechanical Cell Strain Instrument ST-140-04