You are here: Bosch / Facilities / Molecular Biology / Nucleic Acid Analysis

Rotor Gene 6000 Real-Time PCR Machine

Features:

  • Unique centrifugal rotary format
  • Uniformity: ± 0.01°C
  • Resolution: 0.02°C (smallest programmable and reported thermal increment)
  • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels
  • High resolution melt (HRM) application
  • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc
  • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC, and free software upgrades from Qiagen Instrument Support (external website). Select Corbett Rotor-Gene 6000 and then download the application software.

Rotor Gene 3000 Real-Time PCR Machine

Features:

  • Unique centrifugal rotary format
  • Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm
  • Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5)
  • Holds 36 x0.2ml tubes or 72 x 0.1ml strip tubes
  • Software: Build-in extensive analysis, graphing and statistical functions. Free software upgrades via the Qiagen Instrument Support (external website). Select Corbett Rotor-Gene 3000 and then download the application software.

Guidelines:

Back to top

Use of the machines in the Molecular Biology Facility

  • The Rotor-Gene machines are in high demand therefore there is an online booking system (external website). Plan ahead, book time slots and stick to them.
  • Users are responsible for supplying all their own plastic ware and reagents. 0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene.
  • Save files in your own folder in the "Rotor Gene Run File" folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server.
  • You can download a copy of the Rotor-Gene software for free from the Qiagen Instrument Support (external website). However, you will have to register with Qiagen before you can download the software.
Back to top

Tips and addition information

  • You can use the Hybaid PCR machine for your reverse transcription reactions.
  • The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl.
  • The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene.
  • Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method.
  • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year. Please contact Dr. Donna Lai for details.
Back to top