Rotor Gene 6000 Real-Time PCR Machine

Features:

  • Unique centrifugal rotary format
  • Uniformity: ± 0.01°C
  • Resolution: 0.02°C (smallest programmable and reported thermal increment)
  • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels
  • High resolution melt (HRM) application
  • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc
  • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC.
  • Download Rotor Gene 6000 application software here.

Rotor Gene 3000 Real-Time PCR Machine

Features:

  • Unique centrifugal rotary format
  • Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm
  • Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5)
  • Holds 36 x0.2ml tubes or 72 x 0.1ml strip tubes
  • Software: Build-in extensive analysis, graphing and statistical functions.
  • Download Rotor Gene 3000 application software here.

Use of the machines in the Molecular Biology Facility

  • The Rotor-Gene machines are in high demand therefore there is an online booking system (external website). Plan ahead, book time slots and stick to them.
  • Users are responsible for supplying all their own plastic ware and reagents. 0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene.
  • Save files in your own folder in the "Rotor Gene Run File" folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server.

Tips and addition information

  • The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl.
  • The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene.
  • Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method.
  • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year. Please contact Dr. Donna Lai for details.