Hybaid Gradient PCR Machine
Features:
- 96 well plate PCR machine
- Gradient block for optimisation of annealing temperature
- Heated lid for oil free thermal cycling
- Holds one 96 well plate OR 96 x 0.2ml tubes
- Advanced editing, eg adjustable temperature ramping, touch down etc.
Guidelines:
Use of the machine in the Molecular Biology Facility
- The PCR is in high demand therefore there is an online booking system (external website). Plan ahead, book time slots and stick to them
- Users are responsible for supplying all their own plastic ware and reagents
- There are five directories (A-E) for storage of cycling programs. Please keep a track of where you store your program and edit a file in preference to producing a new one
Tips and additional information
- Use this machine for reverse transcription (RT) reactions and for standard PCR (and optimisation)
- During programing - to move to a new stage, enter '0's in each field of a step (like wise for finishing a program).
- Set a hold temperature of 4˚C at the end of your run and leave the time as '0' for incubation for an indefinate time frame
- Try the gradient feature for optimising the annealing temperature of a set of primers - press 'continue' when setting the annealing step - you can have a gradient of up to 15˚C across the block
- The exact temperature of each column of wells can be determined by using the gradient calculator - Under 'Options' > 'Calc'. The results are displayed on two srceens
- Additional advanced editing can be achieved by pressing 'continue' and moving through the screen
- The control tube (vial with mineral oil and probe) should be in position A2 (top row, second from left)