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Roche LightCycler 480 Real-Time PCR Machine

Features:

  • A fully integrated multiwell-plate based real-time PCR machine
  • Flexibly switch between 96- or 384-well plate formats. Recalibration is not required after block exchange.
  • Excitation filters: 440nm, 465nm, 498nm, 533nm and 618nm. Emission filters: 488nm, 510nm, 580nm, 610nm, 640nm and 660nm. You may visit Roche website (external website) for more details.
  • Support all current Real-Time PCR formats and applications (gene expression, SNP analysis, haplotyping, mutation scanning)
  • LightCycler 480 Software (Version 1.5) enables different analysis capabilities for product characterisation (SYBR Green I assays, Tm calling), gene quantification (absolute and relative), and genotyping (end-point or melting curve-based methods)
  • The LightCycler® 480 Multiple Plate Analysis Software allows you to perform large-scale analyses by easily comparing and visualizing PCR data from multiple instrument runs.
  • High Resolution Melting Analysis for screening genetic variations (SNPs, mutations, methylations) in PCR amplicons.
  • Reaction volumes: 10-100ul for 96-well format and 5-20ul for 384-well format
  • Multiwell plates with cut-away corner for error-free positioning and applied bar code for easy assay and sample tracking

Guidelines:

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Use of the machines in the Molecular Biology Facility

  • Please book the machine before use via online booking system (external website).
  • Users are responsible for supplying all their own plastic ware and reagents.
  • Save files in your own folder in the "LightCycer480 Run File" folder in the local disk (C:). No internet access is available in this computer.

Tips and addition information

  • Use hot-start qPCR master mixes to improve the specificity, sensitivity, and yield of qPCR.
  • To achieve optimal assay performance, only use LightCycler 480 Multiwell Plates (white or clear 96-well or 384-well formats are available). You may order it at a discount price through the Bosch Molecular Biology Facility.
  • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year. Please contact Dr. Donna Lai for details.
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