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Protein analysis

PAGE and Western Blotting Apparatus

ANDERSON STUART BUILDING - ROOM N232

  • Bio-Rad Mini-Protean 3 Cell
  • 0.75 mm & 1.5 mm spacer plates
  • 10 and 15-well combs
  • Bio-Rad Mini Trans-Blot
  • Bio-Rad High Current PowerPac
  • Magnetic stirrer
  • Orbital shaker
  • Rocker (at 4˚C)
  • Hypercassette

BLACKBURN BUILDING - ROOM 320

  • Bio-Rad Mini-Protean Tetra Cell System
  • 0.75 mm, 1.0mm & 1.5 mm spacer plates
  • 10 and 15-well combs
  • Bio-Rad Mini Trans-Blot Modules
  • Bio-Rad High Current PowerPac
  • Magnetic stirrer
  • Orbital shaker
  • Rocker

Use of the equipment in the Molecular Biology Facility

  • This equipment is in demand therefore there is an online booking system. Plan ahead, book time slots and stick to them.
  • There are now two of each of the PAGE and transfer cells. When booking use the colour system to select the apparatus you need. If someone is already running a gel or transfer on the HC power supply, you will have to pause the run, add yours and continue to run at the volatage already set. DO NOT change the setting without speaking to the person already using the power supply.
  • Users are responsible for supplying all their own reagents and consumables.

Tips and Additional Information

  • The Mini-PROTEAN 3 cell runs both hand cast gels and Ready Gel precast gels.

Trans-Blot Turbo Transfer System

Bio-Rad Trans-Blot Turbo blotting system is a rapid semi-dry blotting system. The system integrates a high amperage power supply that directs current between a built-in platinized titanium anode and stainless steel cathode. This allows highly efficient and rapid transfer in as little as 3 minutes.

The system is compatible with various gel types and percentages. It can transfer 1-4 mini or 1-2 midi gels in a single run. Either polyvinylidene fluoride (PVDF) or nitrocellulose blotting membranes can be used on the system. Up to 25 protocols can be customized and stored within the instrument.

Guidelines:
The Trans-Blot Turbo blotting system is located in room N232, Anderson Stuart building. Online booking system is available. You need to be trained on how to use the system by the Molecular Biology Officer before you can use it independently. Please clean the system properly after use. Thanks.

SNAP i.d. Protein Detection System

Millipore SNAP i.d. protein detection system is a vacuum-driven system for fast Western blotting. The amount of time required for all immunodetection steps after protein transfer to a membrane, i.e. blocking, washing, and primary and secondary antibody incubations, is about 30 minutes without any loss of signal intensity or reduction in blot quality.

The system is compatible with most standard blocking buffers and visualisation methodologies (e.g. chemiluminescence, fluorescence or colorimetric) and works with polyvinylidene fluoride (PVDF) and nitrocellulose blotting membranes.

Guidelines:
Two SNAP i.d. protein detection systems are located in room 320 (Blackburn) and room N232 (Anderson Stuart building), respectively. Online booking system is available. You need to be trained on how to use the system by the Molecular Biology Officer before you can use it independently. Please empty and clean the waste bottles connected to the SNAP id systems after use. Thanks.

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