Astell Swiftlock Autoclave

Features:

  • 200 Litre capacity
  • Swiftlock closure for safe locking of door during pressurisation - three position bolt

Guidelines:

Use of the machine in the Molecular Biology Facility

  • There is a booking sheet on the door of this autoclave. Please sign in when you put something in the autoclave and sign out when you remove it. This allows the owner of any waste or sterilised equipment and solutions to be idenitifed when it is left behind.
  • This autoclave will not work unless there is sufficient water in the bottom reservoir. Fill using RO water (there is a container on the top - fill this up if you empty it). The machine will tell you when the water level is 'OK'.
  • To start the machine, lock the door (handle down when locked) and press start (hold in for 1 second). Follow this with holding the F3 button for 1 second.
  • The autoclave must cool down before you can open the door, therefore each run will take about 2 hours.
  • To open the machine, hold in the 'door' button for about 1 second. There is a 30 second delay before you can open the door (this counts down), you then have 10 seconds to open the door before it will lock again.
  • If you are sterilising waste, please double bag and DO NOT seal the bags. If your waste makes a mess, you must clean it up - this usually involves draining the machine (yellow tap on back of autoclave), cleaning and refilling the reservoir with RO water (there is a long tube on the shelf above the sink for attaching to the RO tap).
  • PC2 waste should be transported into the facility in a sealed container. The temperature during the sterilisation of PC2 waste must be monitored during every cycle. Most users use a chemical indicator (this needs to be placed inside the waste bag). A biological indictor (e.g. spore test) must be run once a month to determine whether the autoclave is sterilising effectively - the result needs to be posted on the notice attached to the autoclave.

Tips and additional information

If you fill the machine with water to what you think is a reasonable level and it is still telling you that the water level is too low, try one of the following:

  • Put some 4M NaCl in the water (about 5 mls). The electrodes that read the water level rely on the water conducting (which RO water does not do very well).
  • Scrub the electrodes (at the back of the chamber) which detect the water level - they can build up with gunk and not detect. Make sure the high electrode (right) is sticking down into the water.