Ultramicroscope

Image of Ultramicroscope II

An Australia-first! The La Vision BioTec Second Generation Ultramicroscope has arrived at the Bosch Institute Advanced Microscopy Facility.

This Ultramicroscope II is a bidirectional triple light sheet microscope. This latter technology generates 6 focused light sheets to excite samples from the side while the fluorescence light is detected by an sCMOS camera perpendicular to the illumination plane. Moving the sample through the light sheet generates a 3D image stack. Selective excitation of the focal plane reduces bleaching and phototoxicity. The open setup allows the analysis of cleared samples in any clearing solution or in vivo data acquisition in aqueous media. The dynamic horizontal light sheet focus guarantees excellent Z-resolution covering the entire field of view.

More details about this microscope can be provided on request.


Funds for this Ultramicroscope were provided by Clive & Vera Ramaciotti Equipment Grant 2013, Sydney NHMRC Equipment Grant 2013 and DVCR, Anatomy & Histology, Bosch Institute and School of Medical Sciences funding in 2013.

Ultramicroscope II Manual.

Data management information for Ultramicroscope users

One image stack acquired on the Ultramicroscope can contain thousands of slices in a file several gigabytes in size. We advise Ultramicroscope users to utilise digital storage space on the Sydney RDS for their files. Go to the Sydney Research Data Management Planning Tool to create your data management plan and request up to 2 TB digital storage space on the RDS.

Filters installed in the Ultramicroscope

FilterEx

FilterEm

Colour

405/40

460/50

Blue

430/24

480/40

Blue

480/40

525/50

Green

500/20

535/30

Green

545/30

590/50

Red

580/25

625/30

Red

640/30

690/50

Red

710/75

810/90

Red

Ultramicroscope II

Image by Kristie Smith, Brain and Mind Centre.

Neurones in the cerebral cortex of the mouse brain

The areas shown include neocortex (top left) and the hippocampal and dentate gyri (lower right). The area shown is from a 5mm thick slice of a mouse brain, which has been prepared (cleared) for optical sectioning. Image was captured with the Ultramicroscope II. A stack of images was collected and merged to produce this image.

Technical: The mouse is from a strain bred with a marker which makes nerve cells fluoresce brightly. Some of the extensions (dendrites) of the nerve cells are shown. The marker labels the neuron-specific molecule Thy-1.