Image of the Month 2013
This is a transmitted light micrograph of a recently metamorphosed juvenile sea urchin showing the complicated calcareous structures that support the arms and body.
Mammary epithelial cells cultured in 3D matrix to form ductal structures. The cells remodel the matrix surrounding them as the structures form. Cells were stably expressing GFP (green), immunostained E-Cadherin (Red) and co-stained with Hoechst 33342 (Blue).
Image captured on Ultraview spinning-disc confocal microscope (Perkin Elmer).
A section of human airway from a patient without respiratory disease. Second order harmonics were used to visualize fibrillar collagen.
Organised (green) and disorganized (red) collagen and in blue is the autofluorescence/multi-photon excitation fluorescence showing basic tissue structure.
Collagen is present in the extracellular matrix (ECM) throughout the tissue acting as structural support and it has been acknowledged that the ECM is also a bioactive component that can contribute to disease pathology. Image was taken with the Leica Multi-Photon SP5 microscope at 63x magnification.
Final image is stitched from 3 x 3 tiled images.
Mouse Tibialis anterior hindlimb muscle, sectioned through the midbelly region. All muscle fibres within the muscle are delineated by dystrophin stain (green outline). Different types of fibre within the muscle are visualised by fill colour - 2B fibres (red), 2A fibres (green), 2X fibres (no colour).
Muscles are composed of a heterogenous mix of fibre types which are 'fast-twitch' or 'slow-twitch', optimally arranged for speed or endurance, respectively.
Image acquired with a Zeiss Axiovert 200M widefield fluorescence microscope.
The insidious dementias of ageing (Alzheimer’s disease) are believed to be caused by a breakdown of the capillaries of the brain. This image shows the accumulation of the peptide Aβ in the walls of a small-vessel aneurysm, in the neocortex of an aged Alzheimer sufferer. The red label shows surrounding astrocytes, with some abnormal formations. Nuclei are labelled blue.
Image taken at 63x with the Axioplan2 Deconvolution Microscope.