Image of the Month 2014
Osteoclasts are cells that are involved in the degradation of the bone. They can be found residing in pits within the bone, known as the Howship's lacunae. This image, taken with a scanning electron microscope, shows an osteoclast (red) with fine needle-like protrusions contacting the floor of such a pit, on the surface of the bone.
Overlaid immunofluorescence + brightfield images of a day 15 embryoid body. Mouse ES cells were directed down the neurectoderm lineage using time-dependent additions of small molecules, including L-proline, which acts like a growth factor. The neural progenitor marker Nestin (red) shows staining in both the core and outer rind of the body, whilst Oct4 (green) is expressed in patches of primitive neural stem cells. The brightfield shows axonal bundles from mature neurons (top) and immature neural cells migrating away from the core of the body. Yellow staining indicates the colocalisation of Nestin and Oct4, and blue represents the DAPI-stained nuclei.
This image shows CD11b+ (red) inflammatory macrophages infiltrating into the brain in the vicinity of NS-1+ (green) West Nile virus (WNV) infected neurons. In this setting, the macrophages cause pathology and ultimately lead to death in infected animals.
Adipose derived stem cells proliferate over a substrate made from a ‘forest’ of vertically aligned carbon nanotubes.
This is a Crown-of-Thorn starfish bipinnaria larvae, which was stained with a Nile Red solutionto identify and visualise lipid bodies in the larval framework.
This transmission electron micrograph depicts a capillary containing an erythrocyce (E). A pericyte is seen surrounding the endothelial cells on the outside of the capillary (arrow). Collagen fibres of the endometrial stroma are visible in the upper portion of the image.
This image shows active synaptic stripping by microglia in adult human brain of a neurologically normal individual.
Presynaptic vesicles labeled with synaptophysin (red) are visible within the microglial cell labelled with iba1 (green). This suggests microglia monitor the state of synapses in the CNS and contribute to re-modeling and plasticity of neuronal circuits.
This 2-photon fluorescent image shows a Golgi stained prefrontal neuron. The 2-photon laser is powerful enough to excite the cell to fluoresce. This image depicts the neuronal soma in purple and its synaptic spines in orange.
The bone marrow is one of the main hematopoietic sites for blood cell development. However, there are still cell types within the bone marrow that are yet to be identified. This SEM image shows small cells (green) being surrounded by a large cell (blue) found within the murine bone marrow.
Human left ventricular tissue stained with an antibody against the C-terminal end of cardiac obscurin (green), and to connexin-43 (red). Nuclei were stained with DAPI (blue). In this image, obscurin and connexin-43 colocalise to the cardiac intercalated disk (yellow). Taken on a Zeiss 510 Meta Confocal microscope at 10x magnification.
Aged wildtype mouse retina stained with antibodies against Collagen IV (green), Glial Fibrillary Acidic Protein (red) and S100b (blue), showing the interplay between blood vessels, astrocytes and Müller glia respectively. Taken on a Zeiss Zeiss Axio Imager.M2 upright microscope at 20x magnification.
Neural stem cells stained for Nestin, an intermediate filament protein (red); Phalloidin conjugated with Alexa 594 for actin filaments (orange); Beta 3 tubulin, a microtubule expressed in immature neurons (green); and DAPI for nuclei localization (blue).
In this study, we assessed the effects of iron-oxide nanoparticles on cell morphology and cytoskeleton, prior to utilising these cells for in vitro and in vivo detection using Magnetic resonance imaging.