Image of the Month 2017

December - Floral Embryology of the Australian Tomato (Solanum centrale) at Post-fertilisation Stage

Image of the month

Image by Nabil Ahmad, Plant Breeding Institute

This image shows a longitudinal section of the floral constituents at a post-fertilisation stage. Details show a style and many ovules within the ovary. Some ovules show the first zygotic division and early stages of endosperm development.


November - Erythroblastic Islands

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Image by Jia Hao Yeo, Anatomy & Histology

Erythroblastic islands are multi-cellular clusters found within the bone marrow for red blood cell development. They consist of a central macrophage (yellow) surrounded by red blood cell progenitors. This image is a scanning electron micrograph of the erythroblastic island.


October - Human Trachea and the Respiratory Epithelium (H.E.S. Staining)

Image of the month

Image by Filip Braet, Anatomy & Histology

This type of epithelium, i.e. pseudo-stratified, gives the misleading appearance of having several cell layers. All cells are attached to the basement membrane even though not all of the cells reach the surface. The basal cells are replacement cells and lead to columnar epithelial cells. Goblet cells are mucous secreting epithelial cells providing a thin protective layer to the trachea. Note the highly vascularized connective tissue.


September - Quadruple Immunohistochemistry of Paraffin - Embedded Human Retina-choroid cross-sections

Image of the month

Image by Steven Eamegdool, Save Sight Institute

Tissue was stained for ganglion cells [Beta3-tubulin (red)], the inner nuclei cell layer [Tau (green)], photoreceptors [rhodopsin (orange)] and nuclei (blue). Distinct stratified localisation of different cell types can be clearly visualised using multi-marker immuno-staining, which provides us with a more holistic view of cellular changes within the retina. This can be useful for understanding the early events of many eye diseases such as age-related macular degeneration, and enable future development of therapeutic interventions.


August - Cerebral Amyloid Angiopathy in the temporal lobe of a Down Syndrome patient

Image of the month

Image by Monica Vogiatzis, Anatomy & Histology

A section of temporal lobe in a young trisomy-21 adult shows Cerebral Amyloid Angiopathy (CAA), which is a large deposition of amyloid inside blood vessels. This image shows red arteries surrounded and filled with blue amyloid deposition.


July - Divide and Conquer: Tripolar Metaphase in Aggressive Pancreatic Cancer

Image of the month

Image by Leyla Fouani, Pathology

Cancers feature an inordinate number of abnormalities compared to their normal cell counterparts. These rapidly dividing, highly heterogeneous cells are clever, and constantly evolving to grow, spread, and develop strategies to evade death and therapeutics. With cancers rapid division, what was once a highly conserved and carefully controlled process now proceeds largely unchecked, facilitating the deadliness of the disease. This confocal micrograph depicts one such abnormality, a tripolar metaphase event during mitosis. The tripolar spindles are seen segregating the stained chromosomes in cells derived from deadly pancreatic cancer that had metastasised to the liver.


June - Correlative SEM & AFM on Hepatic Endothelial Cells

Image of the month

Image by Filip Braet, Anatomy and Histology

Scanning electron and atomic force microscopy of the porous surface of hepatic endothelial cells. Each individual pore has an average diameter of about 200 nm. The grey image represents scanning electron microscopy (SEM) information while the gold colour image represents corresponding atomic force microscopy (AFM) information of a grouping of pores also known as sieve plates. The same area of interest in both microscopes was located by using scratches as relocation tool.


May - Mounting Tensions: Stress Fibres take Shape

Image of the month

Image by Daisy Shu, Lovicu Group, Anatomy and Histology

During epithelial-mesenchymal transition (EMT), epithelial cells lose their polarity and transdifferentiate into motile and contractile mesenchymal cells. This process involves a reorganisation of the actin cytoskeleton into dynamic stress fibres, enabling cells to transmit contractile forces. Here we see human lens epithelial cells that have undergone EMT following treatment with TGFβ. The cells exhibit strong immunoreactivity for the mesenchymal marker, alpha-smooth muscle actin (red), localising to stress fibre bundles, and a concomitant translocation of the epithelial marker, beta-catenin (green) from the membrane to the perinuclear region. The nucleus is stained with the Hoechst bisbenzimide stain (blue).

Taken on a Zeiss LSM-5Pa confocal microscope.


April - Heart of Darkness

Image of the month

Image by Bobby Boumelhelm, Fraser Group, Physiology

Pictured here is epicardial adipose (fat) tissue imaged under scanning electron microscopy. The pseudo-colouring showcases the adipocytes (olive green) within the extracellular matrix (red) of
epicardial tissue.

Taken with assistance from Jia Hao Yeo.


March - Insulin-producing Beta cells of the Pancreas

Image of the month

Image by Jason Tong, Thorn Group, Physiology

This image of the insulin-producing beta cells of the pancreas was taken using super-resolution STED microscopy. The insulin (labelled red) is found in vesicles in the cytoplasm of the cells.

Green: Arp3 (actin-related protein 3), labelled with, Alexa Fluor 488
Magenta: F-actin labelled with Alexa Fluor 594
Red: Insulin, labelled with Alexa Fluor 633


February - Microstructures between Developing Erythroid cells in the Erythroid Niches

Image of the month

Image by Jia Hao Yeo, Physiology

Erythroid niches are specialized bone marrow niches for erythroid cells. Despite their discovery in the 1950s, little is known about the morphology of these erythroblastic islands. This scanning electron micrograph reveals pilus like structures between the developing erythroid cells and other microstructures that have not previously been described.


January - Transverse Section of a Sperm Flagellum

Image of the month

Image by Sam Dowland, Anatomy and Histology

Transmission electron micrograph of a cross section of a sperm flagellum showing the characteristic “9 + 2” arrangement of microtubules in the axoneme. Nine fused microtubule doublets form a ring surrounding two single microtubules at the centre. Nine mitochondria can be seen to surround the periphery of the axoneme, one for each microtubule doublet.