Introductory Immunohistochemistry

Who is this course for?

Suitable for both beginners and those who would like to refresh their knowledge and practical skills in immunohistochemistry

Who offers the course?

Discipline of Pathology

Level of assumed/required knowledge

This course assumes no prior knowledge of immunohistochemistry and introduces the participants to concepts from the basics.

It is recommended that participants have some prior knowledge of histochemistry or have completed the Introductory Histotechniques course

Course overview

Concepts covered during the lectures include:

  • Fixation for immunohistochemistry
  • Direct and indirect immunostaining
  • Reporter molecules
  • Endogenous enzyme activity
  • Blocking techniques
  • Double labelling
  • Antigen retrieval
  • Choice of controls
  • Trouble shooting

In the practical sessions participants will:

  • Demonstrate smooth muscle actin using the ABC technique
  • Restore cytokeratin immunoreactivity using heat induced antigen retrieval
  • Immunofluorescently demonstrate smooth muscle actin using the indirect technique

Course duration

2 days

How is the course assessed?


Can this course contribute to an academic degree?


Course delivery

The course is completed over two days and consists of a 1 hour lecture each morning followed by hands on practical sessions in the laboratory for the remainder of both days

Course cost

Members of the Central Clinical School - $150.00
Members of the Bosch Institute - $180.00
Other members of the University of Sydney and associated institutes - $250.00
External participants - $700.00
All prices are Ex GST where applicable.

Course dates

2015 dates
Dates to be confirmed

Is this course accredited for CME points?


Further information and registration

For further information visit the pathology website

To register please complete the course registration form and email it to the course co-ordinator.

Contact person/Course coordinator

Ms Sanaz Maleki
Histopathology Laboratory Manager
Discipline of Pathology, Sydney Medical School
Rm 2401, level 2, Charles Perkins Centre
University of Sydney NSW 2006

T: 02 9351 6152

Last updated

January 2015