Suitable for both beginners and those who would like to refresh their knowledge and practical skills in immunohistochemistry
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This course assumes no prior knowledge of immunohistochemistry and introduces the participants to concepts from the basics.
It is recommended that participants have some prior knowledge of histochemistry or have completed the Introductory Histotechniques course
Concepts covered during the lectures include:
- Fixation for immunohistochemistry
- Direct and indirect immunostaining
- Reporter molecules
- Endogenous enzyme activity
- Blocking techniques
- Double labelling
- Antigen retrieval
- Choice of controls
- Trouble shooting
In the practical sessions participants will:
- Demonstrate smooth muscle actin using the ABC technique
- Restore cytokeratin immunoreactivity using heat induced antigen retrieval
- Immunofluorescently demonstrate smooth muscle actin using the indirect technique
The course is completed over two days and consists of a 1 hour lecture each morning followed by hands on practical sessions in the laboratory for the remainder of both days
Members of the Central Clinical School - $150.00
Members of the Bosch Institute - $180.00
Other members of the University of Sydney and associated institutes - $250.00
External participants - $700.00
All prices are Ex GST where applicable.
Dates to be confirmed
Ms Sanaz Maleki
Histopathology Laboratory Manager
Discipline of Pathology, Sydney Medical School
Rm 2401, level 2, Charles Perkins Centre
University of Sydney NSW 2006
T: 02 9351 6152