Cardiovascular & Hormonal Research Laboratory
Lab head: Dr Anastasia Susie Mihailidou
Location: Lvl 13, Kolling Building, Royal North Shore Hospital
Lab members: Ms Loan Le Dr Mahidu Mardini Dr Vincent Wong Mr Harris Mihailidis
Funding: National Heart Foundation & North Shore Heart Research Foundation
Research approach equipment: We will use established techniques in our laboratories recently relocated to the Kolling Building. These include Langendorff apparatus for the ischaemia-reperfusion studies, electrophysiology workstations for patch-clamping heart cells and access to equipment to measure molecular changes.
Buckley T, Mihailidou AS, Bartrop R, McKinley S, Ward C, Morel-Kopp M-C, Spinaze M, Hocking B, Tofler G. Increased blood pressure and heart rate during early bereavement: Potential mechanism of increased cardiovascular risk (submitted Heart Lung & Circulation 2010)
Buckley T, Morel-Kopp M-C, Ward C, Bartrop R, McKinley S, Mihailidou AS, Spinaze M, Chen W, Tofler G. Inflammatory and Thrombotic Changes in Early Bereavement: a Prospective Evaluation (submitted European Heart Journal 2010)
Wong V, Mardini M, Cheung W, Mihailidou AS. High dose insulin in experimental myocardial infarction in rabbits: protection against effects of hyperglycaemia.(In Press, Journal of Diabetes and Its Complications, 4 March2010)
Head G, MihailidouAS, Duggan K, Beilin LJ, BerryN, Brown MA, Bune A, Cowley D, Chalmers JP, Howe PRC, Hodgson J, Ludbrook J, Mangoni AA, McGrath BP, Morley CM, Nelson MR, Sharman JE, Stowasser M. Relationship between ambulatory and Clinic Blood Pressure: Defining diagnostic and treatment targets. (British Medical Journal, Online April 2010)
Mihailidou AS, Le TYL, Mardini M, Funder JW (2009). Glucocorticoids activate cardiac mineralocorticoid receptors during experimental myocardial infarction. Hypertension 54:1306-1312.
Funder JW, Mihailidou AS (2009). Aldosterone and mineralocorticoid receptors: Clinical studies and basic biology. Mol Cell Endocrinol 301: 2-6.
Buckley T, Bartrop R, McKinley S, Ward C, Bramwell M, Roche D, Mihailidou AS, Morel-Kopp M-C, Spinaze M, Hocking B, Goldston K, Tennant C, Tofler G. (2009). A Prospective study of early bereavement on psychological and behavioural cardiac risk factors. Internal Med J 39: 370-378.
Mihailidou AS (2006). Nongenomic actions of aldosterone: Physiological or pathophysiological role? Steroids 71: 277-280.
Mihailidou AS, Funder JW (2005). Nongenomic effects of mineralocorticoid receptor activation in the cardiovascular system. Steroids 70: 347-351.
Mihailidou AS (2005). Review: Nongenomic cardiovascular actions of aldosterone: a receptor for all seasons? Endocrinology 146: 971-972.
Mihailidou AS, Mardini M and Funder JW (2004). Rapid, non-genomic effects of aldosterone in the heart medicated by ePKC. Endocrinology 145: 773-780.
Glucagon-Like Peptide-1 agonists, reperfusion injury and type 2 diabetes
Primary supervisor: Susie Mihailidou
Diabetes is the fastest growing disease worldwide and in the past two decades the prevalence has doubled in Australia, contributing to significant disability, with increased mortality and morbidity from acute myocardial infarction (AMI). Patients with diabetes have a two-fold increase in the risk of short- and long-term mortality after AMI. Current anti-hyperglycemic medications reduce morbidity and mortality in patients with diabetes, however diabetic patients continue to have 2- to 4-fold greater risk of myocardial infarction and heart failure. Additional treatment strategies are needed that prevent these cardiovascular complications in diabetes. The glucagon-like peptide 1 (GLP-1) agonists are an exciting new class of anti-hyperglycaemic medication that not only lower glucose levels, but also reduce weight and have protective effects on pancreatic islets. This project will evaluate the cardiac effects of Liraglutide, a GLP-1 agonist, to protect against AMI using Zucker fatty rats at 12 weeks as a model of type 2 diabetes. Our recent studies show that during myocardial ischemia-reperfusion, cardiac damage is aggravated in Zucker male rats at 12 weeks of age, an established animal model of type 2 diabetes. The studies will involve both functional studies and molecular techniques (immunohistochemistry, western blot analysis).
Co-supervisors: Anthony Ashton
Keywords: Diabetes, Cardiovascular diseases, Reperfusion injury
Contact: Email Susie Mihailidou