Lab head: Professor Jillian Kril
Location: Blackburn Building D06

Our laboratory focuses upon understanding the effects of ageing and dementia.

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Lab members: C Harper (acad), R Pamphlett (acad), J Kril (head), M Buckland (acad), A Harding (acad), R Stankovic (hospstaff), G Sutherland (acad), J Stevens (trc), M Rodriguez (acad)

The effect of the agonal period on the human brain transcriptome

Primary supervisor: Greg Sutherland

The quality of data from global expression analyses (transcriptomics) largely depends on the quality of input RNA. The major determinant of RNA quality in human post-mortem brain tissue is the length and severity of the period just before death (agonal period). Severe agonal periods result in lower pH and RNA quality and many individuals with neurological disorders have prolonged agonal periods compared to individuals who have died in the absence of brain diseases. In gene expression studies that utilise human brain tissue there is a need to separate these agonal effects from the desired ‘disease transcriptome’. This project utilises microarray data derived from a comparison of brain tissue from chronic alcoholic and controls to tease out disease specific-effects using a combination of bioinformatics and quantitative PCR.

Discipline: Pathology