Andrology Laboratory

Lab head: Prof David J Handelsman
Location: ANZAC Research Institute (Concord Hospital)


Website: http://www.anzac.edu.au/
Publications:

The role of androgens in cutaneous wound healing

Primary supervisor: Yiwei Wang

Background: Wound healing is a complicated process which involved three district, overlapping stages: inflammation, new tissue formation and remodeling. Clinically, delayed wound healing is common, particularly in elderly males, suggesting that androgens may play an important role in the healing process.

Using the androgen insensitive, androgen receptor (AR) knockout mouse model (generated using Cre/LoxP system) we have generated preliminary data, suggesting that the AR inactivation accelerates wound healing. The aim of this project is to investigate whether the differences in wound healing could be explained by the AR-mediated, transcriptional regulation of inflammatory factors during the wound healing process.

Objective: To determine the role of androgen-mediated regulation of inflammatory factors in wound healing. The project involves targeted laboratory studies to achieve the objective:
  1. Establishment of skin cells/organ wound healing model in vitro to assess the role of androgens in healing progress.
  2. Examination of wound healing rate and skin regeneration in both AR knockout mice and wide type mice.
  3. Determination of AR-mediated, transcriptional regulation of inflammatory factors during the wound healing process.

Methodology:
  • Skin cells isolation and harvesting from animals and in vitro organ culture
  • Introduction to small animal handling and tissue collection (mouse anatomy, tissue processing).
  • Basic histological techniques: Cutting and handling paraffin sections (microtome use) basic histological staining methods.
  • Immunohistochemistry for detection of proliferative cells within tissue.
  • Molecular biology methods: mRNA extraction, cDNA conversion to generate templates for analysis of mRNA expression levels by real-time or RT-PCR based methods. Western blot or ELISA to determine the inflammatory mediator level in the wound tissue.


Discipline: Pathology
Co-supervisors: Ulla Simanainen
Keywords: Andrology, wound healing
Contact: Email Yiwei Wang