This course assumes no prior knowledge of immunohistochemistry and introduces the participants to concepts from the basics. It is suitable for beginners and those who would like to refresh their knowledge and practical skills in immunohistochemistry.
It is recommended that participants have some prior knowledge of histochemistry or have completed the Introductory Histotechniques course.
The course is completed over 2 days and consists of a 1-hour lecture in the mornings followed by hands-on practical sessions in the laboratory on both days.
Concepts covered during the lectures include:
- Fixation for immunohistochemistry
- Direct or indirect immunostaining
- Reporter molecules
- Endogenous enzyme activity
- Blocking techniques
- Double labelling
- Antigen retrieval
- Choice of controls
In the practical sessions participants will:
- Demonstrate smooth muscle actin using the ABC technique
- Restore cytokeratin immunoreactivity using heat induced antigen retrieval
- Immunofluorescently demonstrate smooth muscle actin using the indirect technique.
Because class size is limited to ten people, there is ample opportunity to tailor certain parts to individual needs. Participants are encouraged to bring along their own antibodies and tissue sections to add to their staining runs or to bring previously attempted slides to troubleshoot them.
- Members of the Central Clinical School: $150.00
- Members of the Bosch Institute: $180.00
- Other members of the University of Sydney and associated institutes: $450.00
- External participants: $1000.00
All prices are ex GST where applicable.
These costs cover all materials used, detailed course notes, and lunch or refreshments.