Dr Anthony Ashton

Level C (Research Only) Academic
Obstetrics, Gynaecology and Neonatology, Northern Clinical School

Telephone +61 2 9926 8486
Fax +61 2 9926 8484

Map

Research interests

Major Research Interests

Angiogenesis Vascular injury and reactivity Cell migration and differentiation Role of small non-protein based mediators in the pathophysiology of disease Narrative Report of Research My principal area of interest in laboratory research lies in the investigation and application of angiogenic regulators in disease states, especially cancer and cardiac re-vascularization. In the past 11 years, I have studied the role of endothelial cell specific receptor isoforms on angiogenesis, examined the complicated relationships between inflammatory mediators on the outcome of angiogenesis and reperfusion injury and looked at the increasingly important role of cardiac specific modulators of apoptosis in the preservation of myocardium and mechanisms that might prevent myocyte apoptosis during reperfusion injury. As a part of these studies we have spanned the entire spectrum of techniques from recombinant protein production to cell culture models. More recently, we have used in vivo models to accurately model complex disease processes, such as angiogenesis, reperfusion injury, tumorigenesis and wound healing, and to take advantage of the power of mouse genetics in these investigations. Our work on endothelial specific regulators of angiogenesis has focused on novel roles for the eicosanoid thromboxane (TX) A2. We found that TXA2 receptor stimulation alone is sufficient to inhibit angiogenesis through antagonism of connexin signaling, important for coordinating endothelial cell migration (an essential component of angiogenesis). Moreover, TXA2 receptor activation directly antagonizes the signaling of the angiogenic growth factors VEGF and FGF-2, both of which are undergoing clinical trials for pro-angiogenic therapy. These results may be vital to the reformulation of this therapy since many diseases recommended for pro-angiogenic therapy, such as re-vascularization of infarcted myocardium, also have locally or systemically elevated TXA2 levels. Further, only the B-isoform of the TXA2 receptor has this anti-angiogenic activity. Humans are the only species in which this receptor has been found and its function has previously been undetermined. Our work on this receptor establishes a novel role for isoform-specific regulation of angiogenesis by TXA2 receptors, provides the first pathophysiological significance of the two isoforms in humans, and clarifies the mechanism by these receptors regulate angiogenesis. This work has substantially altered the current model for the role of thromboxane A2 in vascular biology and has significant clinical implications for an emerging therapeutic regimen. In ischemia-reperfusion injury, we recently described in detail a novel molecular interaction between the signaling pathways of the TXA2 and TNFa receptors that induces cardiac damage in part through limiting the angiogenic response from endothelial cells in the border area of an infarct. In an extension of these findings, we showed deletion of the TXA2 receptor decreases myocardial damage, compared to wild-type mice, in a murine model of cardiac infarction. This increased myocardial health directly correlated with lower rates of myocardial and endothelial cell apoptosis and increased survival. Thus,this phenomenon as one of potential clinical importance and may possibly explain the conflicting data on the role of TNFa in infarction. Finally, we have recently been involved with characterizing the behavior of a very important cardiac-enriched protein, ARC (Apoptosis Repressor with CARD (Caspase Recruitment Domain)), that appears to function as the first master repressor of apoptosis. We have shown that ARC binds to and inhibits several proteins that regulate both the death receptor (extrinsic) and mitochondrial (intrinsic) pathways of apoptosis, including Fas, FADD, and Bax, and endows protection against cell death from stimuli which activate either pathway. Further, the final common pathway of apoptosis, after activation of Caspase 3, is also regulated by ARC. Specifically, ARC inhibits the proteolytic processing and activation of several Caspase-3 substrates responsible for dismantling the nucleus during the final phase of apoptosis. These are some of our most exciting results to date as no other protein has been demonstrated to inhibit nuclear degradation during apoptosis once Caspase 3 is activated.

International links

Ireland

(Department of Biochemistry, Conway Institute of Biomolecular & Biomedical Research, University College, Dublin) Structure-function relationships in prostaglandin receptors Dr. B. Therese Kinsella

Italy

(Lab. of Molecular Pharmacology Dept. Pharmacological Sciences University of Milan) Examining structural motifs in the regulation of thromboxane receptor signalling Dr.s Valerie Capra and G.Enrico Rovati

United States

(Albert Einstein College of Medicine) Consultant to Mechanisms of Disease Training Grant Dr.s Weiss and Tanowitz

United States

(Center for Arrhythmia Research University of Michigan Ann Arbour, MI) Role of connexin 43 in angiogenesis Dr. Mario Delmar

United States

(Department of Medicine; Division of Nephrology Duke University Medical Center Durham, NC) Role in TP in disease Dr. Thomas Coffman

United States

(Department of Molecular Physiology and Biological Physics University of Virginia School of Medicine) Study of the pathophysiological role of connexins in angiogenesis Dr. Brian Duling

United States

(Department of Pathology Montefiore Medical Center Albert Einstein College of Medicine) Dissecting the mechanisms of anti-phospholipid antibodies in coagulation Dr. Jacob Rand

United States

(Dept.s of Radiation Oncology & Pathology Wayne State University Detroit, MI) Study of the pathophysiological role of thromboxane receptors Dr. Kenneth V. Honn

United States

(Institute of Translational Medicine and Therapeutics University of Pennsylvania School of Medicine) Examining the regulation of angiogenesis by thromboxane receptors in vivo Dr.s Garret FitzGerald and Yan Cheng

United States

(University of Kentucky) Study of thromboxane receptor signalling Dr. Hsin-Hsiung Tai

United States

(University of Texas Health Centre) Study of the pathogenic role of TXA2 in cancer and parasitic disease Dr. Kenneth Wu

Selected grants

2014

  • The central role of Connective Tissue Growth Factor in remodelling of asthmatic airways; Burgess J, Ashton A; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Role for the human-specific isoform of the thromboxane A2 receptor (TPB) in the pathogenesis of pre-eclampsia and the cardiovascular complications of pregnancy; Ashton A, Morris J; Heart Research Australia/Research Support.
  • Gap junctions in angiogenesis; Ashton A; DVC Research/Bridging Support Grant.
  • Working towards a new therapy for IUGR; Morris J, McCracken S, Ashton A; National Health and Medical Research Council (NHMRC)/Project Grants.

2013

  • Bridging Support for Senior Scientists - Northern Clinical School; Ashton A; Northern Sydney and Central Coast Area Health Service/Research Support.

2012

  • Regulation of Tumour Development by the Thromboxane A2 Receptor Signalling Axis.; Ashton A; DVC Research/Bridging Support Fellowship.

2011

  • IncuCyte FLR Microscope System and Cell Incubator; Baxter R, Marsh D, Jackson C, O'Neill C, Ashton A, Howell V; Clive & Vera Ramaciotti Foundation/Awards for Biomedical Research: Major Equipment.

2010

  • Population Health at the Clinical Interface: Pregnancy and Childbirth; Haines M, Roberts C, Ford J, Todd A, Simpson J, Ashton A, Taylor L, Morris J, Ellwood D; National Health and Medical Research Council (NHMRC)/Centre of Clinical Research Excellence.
  • Role of the B-Isoform of the thromboxane A2 receptor in breast cancer; Ashton A, Marsh D; North Sydney Central Coast Area Health Services (NSCCAH)/Research Support.
  • In-Vivo Multispectral Imaging FX System: small animal imaging for multi-modal molecular signal localisation in live animals; Marsh D, Baxter R, Ashton A, Howell V, Soon P, Little D, Robinson B; Cancer Institute New South Wales/Equipment Grant.

2009

  • Connexin Isoform Switching Regulates Angiogenesis; Ashton A; National Health and Medical Research Council (NHMRC)/Project Grants.
  • The role of oxygen sensing in regulation of trophoblast invasion; Morris J, Gallery E, Ashton A; National Health and Medical Research Council (NHMRC)/Project Grants.

2008

  • Thromboxane receptor signalling in endothelial cells; Ashton A; National Health and Medical Research Council (NHMRC)/Project Grants.

2006

  • Regulation Of Angiogenesis By Thromboxane Receptor Activation; Ashton A; National Health and Medical Research Council (NHMRC)/Established Career Fellowships.

Selected publications

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Book Chapters

  • Mihailidou, A., Ritchie, R., Ashton, A. (2013). Sex Differences in Sudden Cardiac Death. In David Gaze (Eds.), Ischemic Heart Disease, (pp. 61-75). Croatia: InTech Publishers.
  • Machado, F., Mukherjee, S., Weiss, L., Tanowitz, H., Ashton, A. (2011). Bioactive Lipids in Trypanosoma cruzi Infection. In Louis M. Weiss & Herbert B. Tanowitz (Eds.), Advances in Parasitology (Volume 76): Chagas Disease, Part B, (pp. 1-31). London: Academic Press Elsevier.
  • Ashton, A., D'Alessandro, D., Michler, E. (2006). Use of skeletal myoblasts for the treatment of CHF. In Marc S Penn (Eds.), Stem Cells and Myocardial, (pp. 259-276). United States: Humana Press.
  • Ashton, A., Duffy, H., Iacobas, I., Spray, D. (2005). Using antibody arrays to detect protein-protein interactions in connexins. In Stefan Dhein, Friedrich Wilhelm Mohr & Mario Delmar (Eds.), Practical Methods in Cardiovascular Research, (pp. 916-935). United States of America: Springer.

Journals

  • Schneuer, F., Roberts, C., Ashton, A., Guilbert, C., Tasevski, V., Morris, J., Nassar, N. (2014). Angiopoietin 1 and 2 serum concentrations in first trimester of pregnancy as biomarkers of adverse pregnancy outcomes. American Journal of Obstetrics and Gynecology, 210(4), e1-e9. [More Information]
  • Schneuer, F., Roberts, C., Guilbert, C., Simpson, J., Algert, C., Khambalia, A., Tasevski, V., Ashton, A., Morris, J., Nassar, N. (2014). Effects of maternal serum 25-hydroxyvitamin D concentrations in the first trimester on subsequent pregnancy outcomes in an Australian population1-3. American Journal of Clinical Nutrition, 99(2), 287-295. [More Information]
  • Schneuer, F., Roberts, C., Guilbert, C., Simpson, J., Algert, C., Khambalia, A., Tasevski, V., Ashton, A., Morris, J., Nassar, N. (2014). Maternal serum 25(OH)D concentrations in the first trimester on subsequent pregnancy outcomes in an Australian population. The American Journal of Clinical Nutrition, 99(2), 287-295. [More Information]
  • Le, T., Ashton, A., Mardini, M., Stanton, P., Funder, J., Handelsman, D., Mihailidou, A. (2014). Role of Androgens in Sex Differences in Cardiac Damage During Myocardial Infarction. Endocrinology, 155(2), 568-575. [More Information]
  • Schneuer, F., Nassar, N., Guilbert, C., Tasevski, V., Ashton, A., Morris, J., Roberts, C. (2013). First trimester screening of serum soluble fms-like tyrosine kinase-1 and placental growth factor predicting hypertensive disorders of pregnancy. Pregnancy Hypertension: an international journal of women's cardiovascular health, 3(4), 215-221. [More Information]
  • Mukherjee, S., Sadekar, N., Ashton, A., Huang, H., Spray, D., Lisanti, M., Machado, F., Weiss, L., Tanowitz, H. (2013). Identification of a functional prostanoid-like receptor in the protozoan parasite, Trypanosoma cruzi. Parasitology Research, 112(4), 1417-1425. [More Information]
  • Chaston, D., Baillie, B., Grayson, T., Courjaret, R., Heisler, J., Lau, K., Machaca, K., Nicholson, B., Ashton, A., Matthaei, K., et al (2013). Polymorphism in endothelial connexin40 enhances sensitivity to intraluminal pressure and increases arterial stiffness. Arteriosclerosis, Thrombosis, and Vascular Biology, 33(5), 962-970. [More Information]
  • Schneuer, F., Nassar, N., Khambalia, A., Tasevski, V., Ashton, A., Morris, J., Roberts, C. (2012). First trimester screening of maternal placental protein 13 for predicting preeclampsia and small for gestational age: In-house study and systematic review. Placenta, 33(9), 735-740. [More Information]
  • Le, T., Mardini, M., Howell, V., Funder, J., Ashton, A., Mihailidou, A. (2012). Low-dose spironolactone prevents apoptosis repressor with caspase recruitment domain degradation during myocardial infarction. Hypertension, 59(6), 1164-1169. [More Information]
  • Woolnough, C., Wang, Y., Kan, C., Morris, J., Tasevski, V., Ashton, A. (2012). Source of angiopoietin-2 in the sera of women during pregnancy. Microvascular research, 84(3), 367-374. [More Information]
  • Gavrilov, S., Nuhrenberg, T., Ashton, A., Peng, C., Moore, J., Konstantinidis, K., Mummery, C., Kitsis, R. (2012). Tbx6 is a determinant of cardiac and neural cell fate decisions in multipotent P19CL6 cells. Differentiation, 84(2), 176-184. [More Information]
  • Mukherjee, S., Machado, F., Huang, H., Oz, H., Jelicks, L., Prado, C., Koba, W., Fine, E., Zhao, D., Factor, S., Ashton, A., et al (2011). Aspirin treatment of mice infected with Trypanosoma cruzi and implications for the pathogenesis of Chagas disease. PLoS One, 6(2), e16959-1-e16959-10. [More Information]
  • Jacobs, M., Nassar, N., Roberts, C., Hadfield, R., Morris, J., Ashton, A. (2011). Levels of soluble fms-like tyrosine kinase one in first trimester and outcomes of pregnancy: a systematic review. Reproductive Biology and Endocrinology, 9(June), 77-1-77-8. [More Information]
  • Tanowitz, H., Mukhopadhyay, A., Ashton, A., Lisanti, M., Machado, F., Weiss, L., Mukherjee, S. (2011). Microarray analysis of the mammalian thromboxane receptor-Trypanosoma cruzi interaction. Cell Cycle, 10(7), 1132-1143. [More Information]
  • Hadfield, K., McCracken, S., Ashton, A., Nguyen, T., Morris, J. (2011). Regulated suppression of NF-kB throughout pregnancy maintains a favourable cytokine environment necessary for pregnancy success. Journal of Reproductive Immunology, 89(1), 1-9. [More Information]
  • Nassar, N., Halligan, G., Roberts, C., Morris, J., Ashton, A. (2011). Systematic review of first-trimester vitamin D normative levels and outcomes of pregnancy. American Journal of Obstetrics and Gynecology, 205(3), 208.e1-208.e7. [More Information]
  • Ekambaram, P., Lambiv, W., Cazzolli, R., Ashton, A., Honn, K. (2011). The thromboxane synthase and receptor signaling pathway in cancer: an emerging paradigm in cancer progression and metastasis. Cancer and Metastasis Reviews, 30(3-4), 397-408. [More Information]
  • Rand, J., Wu, X., Quinn, A., Ashton, A., Chen, P., Hathcock, J., Andree, H., Taatjes, D. (2009). Hydroxychloroquine protects the annexin A5 anticoagulant shield from disruption by antiphospholipid antibodies: evidence for a novel effect for an old antimalarial drug. Blood, 115(11), 2292-2299. [More Information]
  • Villalta, F., Scharfstein, J., Ashton, A., Tyler, K., Guan, F., Mukherjee, S., Lima, M., Alvarez, S., Weiss, L., Huang, H., et al (2009). Perspectives on the Trypanosoma cruzi-host cell receptor interactions. Parasitology Research, 104(6), 1251-1260. [More Information]
  • Moussa, O., Ashton, A., Fraig, M., Garrett-Mayer, E., Ghoneim, M., Halushka, P., Watson, D. (2008). Novel Role of Thromboxane Receptors β Isoform in Bladder Cancer Pathogenesis. Cancer Research, 68(11), 4097-4104. [More Information]
  • Muehlbauer, S., Evering, T., Bonuccelli, G., Squires, R., Ashton, A., Porcelli, S., Lisanti, M., Brojatsch, J., Brojatsch, J. (2007). Anthrax lethal toxin kills macrophages in a strain-specific manner by apoptosis or caspase-1-mediated necrosis. Cell Cycle, 6(6), 758-766. [More Information]
  • Zembala, M., Wilczek, P., Kwiatkowski, P., Ashton, A. (2007). Myocardial regeneration – the revolution continues. Kardiochirurgia i Torakochirurgia Polska, 4(3), 239-245.
  • Foo, R., Nam, Y., Ostreicher, M., Metzl, M., Whelan, R., Peng, C., Ashton, A., Fu, W., Mani, K., Chin, S., et al (2007). Regulation of p53 tetramerization and nuclear export by ARC. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 104(52), 20826-20831. [More Information]
  • Nam, Y., Mani, K., Wu, L., Peng, C., Calvert, J., Foo, R., Krishnamurthy, B., Miao, W., Ashton, A., Lefer, D., et al (2007). The apoptosis inhibitor ARC undergoes ubiquitin-proteasomal-mediated degradation in response to death stimuli: identification of a degradation-resistant mutant. Journal of Biological Chemistry, 282(8), 5522-5528. [More Information]
  • Ashton, A., Mukherjee, S., Nagajyothi, F., Huang, H., Braunstein, V., Desruisseaux, M., Factor, S., Lopez, L., Berman, J., Wittner, M., et al (2007). Thromboxane A2 is a key regulator of pathogenesis during Trypanosoma cruzi infection. The Journal of Experimental Medicine, 204(4), 929-940. [More Information]
  • Mercier, I., Vuolo, M., Madan, R., Xue, X., Levalley, A., Ashton, A., Jasmin, J., Czaja, M., Lin, E., Armstrong, R., et al (2005). ARC, an apoptosis suppressor limited to terminally differentiated cells, is induced in human breast cancer and confers chemo- and radiation-resistance. Cell Death and Differentiation, 12(6), 682-686. [More Information]
  • Colombo, P., Banchs, J., Celaj, S., Talreja, A., Lachmann, J., Malla, S., DuBois, N., Ashton, A., Latif, F., Jorde, U., et al (2005). Endothelial cell activation in patients with decompensated heart failure. Circulation, 111(1), 58-62. [More Information]
  • Nam, Y., Mani, K., Ashton, A., Peng, C., Krishnamurthy, B., Hayakawa, Y., Lee, P., Korsmeyer, S., Kitsis, R. (2004). Inhibition of Both the Extrinsic and Intrinsic Death Pathways through Nonhomotypic Death-Fold Interactions. Molecular Cell, 15(6), 901-912. [More Information]
  • Duffy, H., Ashton, A., O'Donnell, P., Coombs, W., Taffet, S., Delmar, M., Spray, D. (2004). Regulation of connexin43 protein complexes by intracellular acidification. Circulation Research, 94(2), 215-222. [More Information]
  • Ashton, A., Cheng, Y., Helisch, A., Ware, J. (2004). Thromboxane A2 Receptor Agonists Antagonize the Proangiogenic Effects of Fibroblast Growth Factor-2:Role of Receptor Internalization, Thrombospondin-1, and αvβ3. Circulation Research, 94(6), 735-742. [More Information]
  • Ashton, A., Ware, J. (2004). Thromboxane A2 receptor signaling inhibits vascular endothelial growth factor-induced endothelial cell differentiation and migration. Circulation Research, 95(4), 372-379. [More Information]
  • Woodman, S., Ashton, A., Schubert, W., Lee, H., Williams, T., Medina, F., Wyckoff, J., Combs, T., Lisanti, M. (2003). Caveolin-1 knockout mice show an impaired angiogenic response to exogenous stimuli. The American Journal of Pathology: cellular and molecular biology of disease, 162(6), 2059-2068. [More Information]
  • Neumeister, P., Pixley, F., Xiong, Y., Xie, H., Wu, K., Ashton, A., Cammer, M., Chan, A., Symons, M., Stanley, E., et al (2003). Cyclin D1 governs adhesion and motility of macrophages. Molecular Biology of the Cell, 14(5), 2005-2015. [More Information]
  • Ashton, A., Ware, G., Kaul, D., Ware, J. (2003). Inhibition of tumor necrosis factor alpha-mediated NFkappaB activation and leukocyte adhesion, with enhanced endothelial apoptosis, by G protein-linked receptor (TP) ligands. Journal of Biological Chemistry, 278(14), 11858-11866. [More Information]
  • Hotchkiss, K., Ashton, A., Klein, R., Lenzi, M., Zhu, G., Schwartz, E. (2003). Mechanisms by Which Tumor Cells and Monocytes Expressing the Angiogenic Factor Thymidine Phosphorylase Mediate Human Endothelial Cell Migration. Cancer Research, 63(2), 527-533. [More Information]
  • Ma, H., Calderon, T., Kessel, T., Ashton, A., Berman, J. (2003). Mechanisms of hepatocyte growth factor-mediated vascular smooth muscle cell migration. Circulation Research, 93(11), 1066-1073. [More Information]
  • Hotchkiss, K., Ashton, A., Schwartz, E. (2003). Thymidine phosphorylase and 2-deoxyribose stimulate human endothelial cell migration by specific activation of the integrins alpha 5 beta 1 and alpha V beta 3. Journal of Biological Chemistry, 278(21), 19272-19279. [More Information]
  • Mani, K., Ashton, A., Kitsis, R. (2002). Taking the BAD out of Adrenergic Stimulation. Journal of Molecular and Cellular Cardiology, 34, 709-712.

2014

  • Schneuer, F., Roberts, C., Ashton, A., Guilbert, C., Tasevski, V., Morris, J., Nassar, N. (2014). Angiopoietin 1 and 2 serum concentrations in first trimester of pregnancy as biomarkers of adverse pregnancy outcomes. American Journal of Obstetrics and Gynecology, 210(4), e1-e9. [More Information]
  • Schneuer, F., Roberts, C., Guilbert, C., Simpson, J., Algert, C., Khambalia, A., Tasevski, V., Ashton, A., Morris, J., Nassar, N. (2014). Effects of maternal serum 25-hydroxyvitamin D concentrations in the first trimester on subsequent pregnancy outcomes in an Australian population1-3. American Journal of Clinical Nutrition, 99(2), 287-295. [More Information]
  • Schneuer, F., Roberts, C., Guilbert, C., Simpson, J., Algert, C., Khambalia, A., Tasevski, V., Ashton, A., Morris, J., Nassar, N. (2014). Maternal serum 25(OH)D concentrations in the first trimester on subsequent pregnancy outcomes in an Australian population. The American Journal of Clinical Nutrition, 99(2), 287-295. [More Information]
  • Le, T., Ashton, A., Mardini, M., Stanton, P., Funder, J., Handelsman, D., Mihailidou, A. (2014). Role of Androgens in Sex Differences in Cardiac Damage During Myocardial Infarction. Endocrinology, 155(2), 568-575. [More Information]

2013

  • Schneuer, F., Nassar, N., Guilbert, C., Tasevski, V., Ashton, A., Morris, J., Roberts, C. (2013). First trimester screening of serum soluble fms-like tyrosine kinase-1 and placental growth factor predicting hypertensive disorders of pregnancy. Pregnancy Hypertension: an international journal of women's cardiovascular health, 3(4), 215-221. [More Information]
  • Mukherjee, S., Sadekar, N., Ashton, A., Huang, H., Spray, D., Lisanti, M., Machado, F., Weiss, L., Tanowitz, H. (2013). Identification of a functional prostanoid-like receptor in the protozoan parasite, Trypanosoma cruzi. Parasitology Research, 112(4), 1417-1425. [More Information]
  • Chaston, D., Baillie, B., Grayson, T., Courjaret, R., Heisler, J., Lau, K., Machaca, K., Nicholson, B., Ashton, A., Matthaei, K., et al (2013). Polymorphism in endothelial connexin40 enhances sensitivity to intraluminal pressure and increases arterial stiffness. Arteriosclerosis, Thrombosis, and Vascular Biology, 33(5), 962-970. [More Information]
  • Mihailidou, A., Ritchie, R., Ashton, A. (2013). Sex Differences in Sudden Cardiac Death. In David Gaze (Eds.), Ischemic Heart Disease, (pp. 61-75). Croatia: InTech Publishers.

2012

  • Schneuer, F., Nassar, N., Khambalia, A., Tasevski, V., Ashton, A., Morris, J., Roberts, C. (2012). First trimester screening of maternal placental protein 13 for predicting preeclampsia and small for gestational age: In-house study and systematic review. Placenta, 33(9), 735-740. [More Information]
  • Le, T., Mardini, M., Howell, V., Funder, J., Ashton, A., Mihailidou, A. (2012). Low-dose spironolactone prevents apoptosis repressor with caspase recruitment domain degradation during myocardial infarction. Hypertension, 59(6), 1164-1169. [More Information]
  • Woolnough, C., Wang, Y., Kan, C., Morris, J., Tasevski, V., Ashton, A. (2012). Source of angiopoietin-2 in the sera of women during pregnancy. Microvascular research, 84(3), 367-374. [More Information]
  • Gavrilov, S., Nuhrenberg, T., Ashton, A., Peng, C., Moore, J., Konstantinidis, K., Mummery, C., Kitsis, R. (2012). Tbx6 is a determinant of cardiac and neural cell fate decisions in multipotent P19CL6 cells. Differentiation, 84(2), 176-184. [More Information]

2011

  • Mukherjee, S., Machado, F., Huang, H., Oz, H., Jelicks, L., Prado, C., Koba, W., Fine, E., Zhao, D., Factor, S., Ashton, A., et al (2011). Aspirin treatment of mice infected with Trypanosoma cruzi and implications for the pathogenesis of Chagas disease. PLoS One, 6(2), e16959-1-e16959-10. [More Information]
  • Machado, F., Mukherjee, S., Weiss, L., Tanowitz, H., Ashton, A. (2011). Bioactive Lipids in Trypanosoma cruzi Infection. In Louis M. Weiss & Herbert B. Tanowitz (Eds.), Advances in Parasitology (Volume 76): Chagas Disease, Part B, (pp. 1-31). London: Academic Press Elsevier.
  • Jacobs, M., Nassar, N., Roberts, C., Hadfield, R., Morris, J., Ashton, A. (2011). Levels of soluble fms-like tyrosine kinase one in first trimester and outcomes of pregnancy: a systematic review. Reproductive Biology and Endocrinology, 9(June), 77-1-77-8. [More Information]
  • Tanowitz, H., Mukhopadhyay, A., Ashton, A., Lisanti, M., Machado, F., Weiss, L., Mukherjee, S. (2011). Microarray analysis of the mammalian thromboxane receptor-Trypanosoma cruzi interaction. Cell Cycle, 10(7), 1132-1143. [More Information]
  • Hadfield, K., McCracken, S., Ashton, A., Nguyen, T., Morris, J. (2011). Regulated suppression of NF-kB throughout pregnancy maintains a favourable cytokine environment necessary for pregnancy success. Journal of Reproductive Immunology, 89(1), 1-9. [More Information]
  • Nassar, N., Halligan, G., Roberts, C., Morris, J., Ashton, A. (2011). Systematic review of first-trimester vitamin D normative levels and outcomes of pregnancy. American Journal of Obstetrics and Gynecology, 205(3), 208.e1-208.e7. [More Information]
  • Ekambaram, P., Lambiv, W., Cazzolli, R., Ashton, A., Honn, K. (2011). The thromboxane synthase and receptor signaling pathway in cancer: an emerging paradigm in cancer progression and metastasis. Cancer and Metastasis Reviews, 30(3-4), 397-408. [More Information]

2009

  • Rand, J., Wu, X., Quinn, A., Ashton, A., Chen, P., Hathcock, J., Andree, H., Taatjes, D. (2009). Hydroxychloroquine protects the annexin A5 anticoagulant shield from disruption by antiphospholipid antibodies: evidence for a novel effect for an old antimalarial drug. Blood, 115(11), 2292-2299. [More Information]
  • Villalta, F., Scharfstein, J., Ashton, A., Tyler, K., Guan, F., Mukherjee, S., Lima, M., Alvarez, S., Weiss, L., Huang, H., et al (2009). Perspectives on the Trypanosoma cruzi-host cell receptor interactions. Parasitology Research, 104(6), 1251-1260. [More Information]

2008

  • Moussa, O., Ashton, A., Fraig, M., Garrett-Mayer, E., Ghoneim, M., Halushka, P., Watson, D. (2008). Novel Role of Thromboxane Receptors β Isoform in Bladder Cancer Pathogenesis. Cancer Research, 68(11), 4097-4104. [More Information]

2007

  • Muehlbauer, S., Evering, T., Bonuccelli, G., Squires, R., Ashton, A., Porcelli, S., Lisanti, M., Brojatsch, J., Brojatsch, J. (2007). Anthrax lethal toxin kills macrophages in a strain-specific manner by apoptosis or caspase-1-mediated necrosis. Cell Cycle, 6(6), 758-766. [More Information]
  • Zembala, M., Wilczek, P., Kwiatkowski, P., Ashton, A. (2007). Myocardial regeneration – the revolution continues. Kardiochirurgia i Torakochirurgia Polska, 4(3), 239-245.
  • Foo, R., Nam, Y., Ostreicher, M., Metzl, M., Whelan, R., Peng, C., Ashton, A., Fu, W., Mani, K., Chin, S., et al (2007). Regulation of p53 tetramerization and nuclear export by ARC. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 104(52), 20826-20831. [More Information]
  • Nam, Y., Mani, K., Wu, L., Peng, C., Calvert, J., Foo, R., Krishnamurthy, B., Miao, W., Ashton, A., Lefer, D., et al (2007). The apoptosis inhibitor ARC undergoes ubiquitin-proteasomal-mediated degradation in response to death stimuli: identification of a degradation-resistant mutant. Journal of Biological Chemistry, 282(8), 5522-5528. [More Information]
  • Ashton, A., Mukherjee, S., Nagajyothi, F., Huang, H., Braunstein, V., Desruisseaux, M., Factor, S., Lopez, L., Berman, J., Wittner, M., et al (2007). Thromboxane A2 is a key regulator of pathogenesis during Trypanosoma cruzi infection. The Journal of Experimental Medicine, 204(4), 929-940. [More Information]

2006

  • Ashton, A., D'Alessandro, D., Michler, E. (2006). Use of skeletal myoblasts for the treatment of CHF. In Marc S Penn (Eds.), Stem Cells and Myocardial, (pp. 259-276). United States: Humana Press.

2005

  • Mercier, I., Vuolo, M., Madan, R., Xue, X., Levalley, A., Ashton, A., Jasmin, J., Czaja, M., Lin, E., Armstrong, R., et al (2005). ARC, an apoptosis suppressor limited to terminally differentiated cells, is induced in human breast cancer and confers chemo- and radiation-resistance. Cell Death and Differentiation, 12(6), 682-686. [More Information]
  • Colombo, P., Banchs, J., Celaj, S., Talreja, A., Lachmann, J., Malla, S., DuBois, N., Ashton, A., Latif, F., Jorde, U., et al (2005). Endothelial cell activation in patients with decompensated heart failure. Circulation, 111(1), 58-62. [More Information]
  • Ashton, A., Duffy, H., Iacobas, I., Spray, D. (2005). Using antibody arrays to detect protein-protein interactions in connexins. In Stefan Dhein, Friedrich Wilhelm Mohr & Mario Delmar (Eds.), Practical Methods in Cardiovascular Research, (pp. 916-935). United States of America: Springer.

2004

  • Nam, Y., Mani, K., Ashton, A., Peng, C., Krishnamurthy, B., Hayakawa, Y., Lee, P., Korsmeyer, S., Kitsis, R. (2004). Inhibition of Both the Extrinsic and Intrinsic Death Pathways through Nonhomotypic Death-Fold Interactions. Molecular Cell, 15(6), 901-912. [More Information]
  • Duffy, H., Ashton, A., O'Donnell, P., Coombs, W., Taffet, S., Delmar, M., Spray, D. (2004). Regulation of connexin43 protein complexes by intracellular acidification. Circulation Research, 94(2), 215-222. [More Information]
  • Ashton, A., Cheng, Y., Helisch, A., Ware, J. (2004). Thromboxane A2 Receptor Agonists Antagonize the Proangiogenic Effects of Fibroblast Growth Factor-2:Role of Receptor Internalization, Thrombospondin-1, and αvβ3. Circulation Research, 94(6), 735-742. [More Information]
  • Ashton, A., Ware, J. (2004). Thromboxane A2 receptor signaling inhibits vascular endothelial growth factor-induced endothelial cell differentiation and migration. Circulation Research, 95(4), 372-379. [More Information]

2003

  • Woodman, S., Ashton, A., Schubert, W., Lee, H., Williams, T., Medina, F., Wyckoff, J., Combs, T., Lisanti, M. (2003). Caveolin-1 knockout mice show an impaired angiogenic response to exogenous stimuli. The American Journal of Pathology: cellular and molecular biology of disease, 162(6), 2059-2068. [More Information]
  • Neumeister, P., Pixley, F., Xiong, Y., Xie, H., Wu, K., Ashton, A., Cammer, M., Chan, A., Symons, M., Stanley, E., et al (2003). Cyclin D1 governs adhesion and motility of macrophages. Molecular Biology of the Cell, 14(5), 2005-2015. [More Information]
  • Ashton, A., Ware, G., Kaul, D., Ware, J. (2003). Inhibition of tumor necrosis factor alpha-mediated NFkappaB activation and leukocyte adhesion, with enhanced endothelial apoptosis, by G protein-linked receptor (TP) ligands. Journal of Biological Chemistry, 278(14), 11858-11866. [More Information]
  • Hotchkiss, K., Ashton, A., Klein, R., Lenzi, M., Zhu, G., Schwartz, E. (2003). Mechanisms by Which Tumor Cells and Monocytes Expressing the Angiogenic Factor Thymidine Phosphorylase Mediate Human Endothelial Cell Migration. Cancer Research, 63(2), 527-533. [More Information]
  • Ma, H., Calderon, T., Kessel, T., Ashton, A., Berman, J. (2003). Mechanisms of hepatocyte growth factor-mediated vascular smooth muscle cell migration. Circulation Research, 93(11), 1066-1073. [More Information]
  • Hotchkiss, K., Ashton, A., Schwartz, E. (2003). Thymidine phosphorylase and 2-deoxyribose stimulate human endothelial cell migration by specific activation of the integrins alpha 5 beta 1 and alpha V beta 3. Journal of Biological Chemistry, 278(21), 19272-19279. [More Information]

2002

  • Mani, K., Ashton, A., Kitsis, R. (2002). Taking the BAD out of Adrenergic Stimulation. Journal of Molecular and Cellular Cardiology, 34, 709-712.

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