Associate Professor Guy Lyons

Clinical Associate Professor
Dermatology, Central Clinical School

Telephone +61 2 9036 6314
Fax 9036 5130

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Research interests

Dr Lyons' research aims to determine the cellular and molecular mechanisms by which oncogenes and tumour suppressor genes contribute to the formation of squamous cell carcinomas of the skin and of the head and neck region, two of the most common sites for cancer. We are interested in the mechanisms involved in the progression from benign tumours, which are non-invasive and usually easily treated, to malignant tumours, which spread invasively from the site of origin and are difficult to treat successfully.

Cancer is a disease of mutating genes, and evolutionary principles apply to it. We are investigating the roles of genes in the ecology of tumours: those that regulate the interactions of cancer cells with each other, with non-malignant stromal cells and with the extracellular matrix. We are particularly interested in the genetic basis of symbiotic relationships between tumour clones that drive invasion and metastasis. In collaboration with investigators at the NIH, University of Queensland and University of Sydney, we have developed novel in silico, in vitro and in vivo models for exploring the nature of clonal evolution during carcinoma development and the evolutionary consequences of expressing particular classes of cancer associated genes.

This research encompasses a broad range of molecular and cell biological methods and analyses, including cell culture, plasmid and virus vector construction, gene expression analysis, small animal handling, live-cell imaging and intravital microscopy.

International links

United States

(National Institutes of Health) Mechanisms of head and neck cancer progression

Selected grants

2014

  • La Vision BioTec Ultramicroscope; Murphy C, Braet F, Byrne M, Chan-Ling T, Fraser S, Goldsbury C, Halliday G, Hambly B, Johnstone D, Keay K, Lovelace M, Lyons G, Mason R, Overall R, Owens T, Richardson D, Stone J, Weiss A; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • CLARIOstar Multifunctional Microplate Reader for Shared Used at the Open Access, Multi-User Molecular Biology Core Facility; Huang M, Scolyer R, Fraser S, Sahni S, Speranza T, Owens T, Barrs V, Ju Y, Kovacevic Z, Lu Z, Camp A, Slobedman B, Morris B, Oliver B, Dos Remedios C, Kalinowski D, Fu D, Lai D, Lovicu F, Allbutt H, Triccas J, Burgess J, Kril J, Dixon K, Rendina L, Byrne M, King N, Groundwater P, Williamson P, Ho J, Dong Q, Bao B, Assinder S, McLennan S, Richardson D, Ammit A, Sharland A, Hardikar A, Hong A, Hambly B, Lee C, Murphy C, Goldsbury C, Johnstone D, Lane D, Hibbs D, Joshua D, Huq F, Halliday G, Sutherland G, Zreiqat H, Lok H, Lyons G, Jansson P, Black J, Zhu L, Sunde M, Day M, Naylor M, Buckland M, Murray M, Shafie N, Lay P, Poronnik P, Codd R, Mason R, Ryan R, Christopherson R, de Graaf S; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2013

  • Robotic High Throughput Western Analysis for the Open Access, Multi-User Sydney Cancer Research Core Facility; Richardson D, Scolyer R, Boyer M, Halliday G, Damian D, Christopherson R, Joshua D, Kench J, Hong A, Murray M, Lee C, Kalinowski D, Naylor M, Lay P, Lyons G, Kovacevic Z, Mason R, Dixon K, Chan-Ling T, Hawkins C, Sunde M, Lovejoy D, Owens T, Rendina L, Jansson P, Dos Remedios C, Charles (nee Slaviero) K, Lane D, Witting P, Dong Q, Ammit A, Groundwater P, Assinder S, Bao B, Byrne S, Zhou F, Buckland M, Grewal T, Huq F, Lai D, Codd R, Zhang D, Fu D, de Graaf S, Huang M, Payne R, Slobedman B, Barrs V, Ho J, Williamson P, Murphy C; DVC Research/Equipment Grant.
  • QX100 Droplet Digital PCR system for Shared Used at the Open Access, Multi-User Molecular Biology Core Facility; Richardson D, Kril J, Murphy C, Vandenberg R, Rendina L, Dos Remedios C, Ju Y, Kovacevic Z, Lyons G, Sutherland G, Lay P, Ammit A, Lai D, Morris B, Day M, Dong Q, Bao B, Huang M, Camp A, Boyer M, Whittington R, Byrne M, Hanrahan J, Fu D, Jansson P, Lane D, Bagley E, Hibbs D, Zhang D, Fraser S, Scolyer R, Black J, Halliday G, Christie M, Zreiqat H, Murray M, Slobedman B, Naylor M, Burgess J, Triccas J, Mason R, Chadban S, Combes V, Lovicu F, McLennan S, Kalinowski D, Sharland A, Johnstone D, Groundwater P, Dixon K, Zhou F, Lovejoy D, Lu Z, Zhu L, Goldsbury C, Witting P, Buckland M, Assinder S, Purdie (Williams) A, Codd R, Owens T, Hambly B; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Tracking epidermal clonal evolution during skin cancer induction and progression; Lyons G, Kiarash K; National Health and Medical Research Council (NHMRC)/Project Grants.

2012

  • Improving the outcomes of oropharyngeal cancer: specific human papillomavirus oncoproteins as modifying factors; Hong A, Lee C, Lyons G, Veillard A, Zhang X; Sydney Catalyst Translational Cancer Research/Pilot and Seed Funding.
  • Interactions between genes that cause cancer; Lyons G, Halliday G; National Health and Medical Research Council (NHMRC)/Project Grants.

2011

  • Susceptibility of the basal layer of human epidermis to UVA oxidative damage due to pheomelanin and suboptimal DNA repair; Halliday G, Lyons G; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Cell cooperation in cancer; Lyons G, Myerscough M; Human Frontier Science Program (France)/Research Support.
  • Clonal cooperation in cancer; Lyons G; Lifehouse at RPA/Research Support.
  • The small animal bioimager; Halliday G, Hambley T, Zreiqat H, Richardson D, Holst J, Rasko J, Byrne S, Dong Q, Hong A, Lyons G; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2010

  • Regulation of keratinocyte differentiation; Lyons G; DVC Research/Bridging Support Grant.
  • Brm and Brg-1 protect from ultraviolet radiation-induced skin and ocular damage; Halliday G, Lyons G, Di Girolamo N, Moloney F, Lee C; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Restoring epithelial differentiation to squamous cell carcinomas; Lyons G, Halliday G; Cancer Council New South Wales/Research Project Grants.
  • Next-generation high-speed flow cytometric cell sorter; Fazekas de St Groth B, King N, Simpson S, Britton W, Campbell I, Vadas M, Grau G, Weninger W, Christopherson R, Gamble J, Rasko J, McCaughan G, Xia P, Beale P, Holst J, Haass N, Combes V, Saunders B, Byrne S, West N, Gorrell M, Bertolino P, Bowen D, Warner F, Seth D, Grimshaw M, Jolly C, Martiniello-Wilks R, Mrass P, Lyons G, Sword G; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2009

  • Polyclonality in cancer; Lyons G, Halliday G, Weninger W; Cancer Institute New South Wales/Research Innovation Grants.

2007

  • Regulation of keratinocyte differentiation by snail; Lyons G; Cancer Council New South Wales/Research Project Grants.
  • Total internal reflectance fluorescence microscope (TIRFM); Soon L, Rendina L, Braet F, Weiss A, Grau G, Lyons G; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2006

  • Role of brm in skin tumour progression from benign to malignant; Halliday G, Lyons G; National Health and Medical Research Council (NHMRC)/Project Grants.

Selected publications

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Book Chapters

  • Chien, H., Fok, S., Raviraj, V., Lee, J., Cole, L., Lyons, G., Soon, L. (2012). Matrix Scaffolds in Cancer Cell Biology; Physiological Representation and Nanoscale Characterisation. In Raja Srirajaskanthan, Victor R. Preedy (Eds.), Nanomedicine and Cancer, (pp. 142-159). Enfield, New Hampshire: Science Publishers Inc.

Journals

  • Hassan, N., Painter, N., Howlett, C., Farrell, A., Di Girolamo, N., Lyons, G., Halliday, G. (2014). Brm Inhibits the Proliferative Response of Keratinocytes and Corneal Epithelial Cells to Ultraviolet Radiation-Induced Damage. PLoS One, 9(9), e107931. [More Information]
  • Tian, X., Zhang, J., Tan, T., Lyons, G., Zhao, H., Niu, B., Lee, S., Tsatralis, T., Zhao, Y., Wang, Y., Cao, Q., Wang, C., Wang, Y., Lee, V., Zheng, G., Harris, D., et al (2013). Association of beta catenin with P-Smad3 but not LEF-1 dissociates in vitro profibrotic from anti-inflammatory effects of TGF- beta1. Journal of Cell Science, 126(1), 67-76. [More Information]
  • Jung, M., Lord, M., Cheng, B., Lyons, G., Alkhouri, H., Hughes, M., McCarthy, S., Iozzo, R., Whitelock, J. (2013). Mast Cells Produce Novel Shorter Forms of Perlecan That Contain Functional Endorepellin: A Role in Angiogenesis and Wound Healing. Journal of Biological Chemistry, 288(5), 3289-3304. [More Information]
  • Raviraj, V., Fok, S., Zhao, J., Chien, H., Lyons, G., Thompson, E., Soon, L. (2012). Regulation of ROCK1 via Notch1 during breast cancer cell migration into dense matrices. BMC Cell Biology, 13(5), 1-17. [More Information]
  • Halliday, G., Zhou, Y., Sou, P., Huang, X., Rana, S., Bugeja, M., Painter, N., Scolyer, R., Muchardt, C., Di Girolamo, N., Lyons, G. (2012). The absence of Brm exacerbates photocarcinogenesis. Experimental Dermatology, 21(8), 599-604. [More Information]
  • Bock, V., Lyons, G., Huang, X., Jones, A., McDonald, L., Scolyer, R., Moloney, F., Barnetson, R., Halliday, G. (2011). BRM and BRG1 subunits of the SWI�?�SNF chromatin remodelling complex are downregulated upon progression of benign skin lesions into invasive tumours. British Journal of Dermatology, 164(6), 1221-1227. [More Information]
  • Farrell, A., Halliday, G., Lyons, G. (2011). Chromatin Structure Following UV-Induced DNA Damage-Repair or Death? International Journal of Molecular Sciences, 12(11), 8063-8085. [More Information]
  • Javeri, A., Lyons, G., Huang, X., Halliday, G. (2011). Downregulation of cockayne syndrome B protein reduces human 8-oxoguanine DNA glycostlase-1 expression and repair of ultraviolet radiation-induced 8-oxo-7,8-dihydro-2'-deoxyguanine. Cancer science, 102(9), 1651-1658. [More Information]
  • Pang, E., Delic (nee Roue), N., Hong, A., Zhang, M., Rose, B., Lyons, G. (2011). Radiosensitization of Oropharyngeal Squamous Cell Carcinoma Cells By Human Papillomavirus 16 Oncoprotein E6*I. International Journal of Radiation: Oncology - Biology - Physics, 79(3), 860-865. [More Information]
  • Sou, P., Delic (nee Roue), N., Halliday, G., Lyons, G. (2010). Snail transcription factors in keratinocytes: enough to make your skin crawl. The International Journal of Biochemistry and Cell Biology, 42(12), 1940-1944. [More Information]
  • Zheng, G., Lyons, J., Tan, T., Wang, Y., Hsu, T., Min, D., Succar, L., Rangan, G., Hu, M., Henderson, B., Alexander, S., Harris, D. (2009). Disruption of E-Cadherin by Matrix Metalloproteinase Directly Mediates Epithelial-Mesenchymal Transition Downstream of Transforming Growth Factor-{beta}1 in Renal Tubular Epithelial Cells. The American Journal of Pathology: cellular and molecular biology of disease, 175(2), 580-591. [More Information]
  • Min, D., Lyons, G., Bonner, J., Twigg, S., Yue, D., McLennan, S. (2009). Mesangial cell-derived factors alter monocyte activation and function through inflammatory pathways: possible pathogenic role in diabetic nephropathy. American Journal of Physiology: Renal Physiology (Print), 297(5), F1229-F1237. [More Information]
  • Halliday, G., Bock, V., Moloney, F., Lyons, G. (2009). SWI/SNF: A chromatin-remodelling complex with a role in carcinogenesis. The International Journal of Biochemistry and Cell Biology, 41(4), 725-728. [More Information]
  • Moloney, F., Lyons, G., Bock, V., Huang, X., Bugeja, M., Halliday, G. (2008). Hotspot Mutation of Brahma in Non-Melanoma Skin Cancer. Journal of Investigative Dermatology, 129(4), 1012-1015. [More Information]
  • Halliday, G., Lyons, G. (2008). Inflammatory Doses of UV May Not Be Necessary for Skin Carcinogenesis. Photochemistry and Photobiology, 84(2), 272-283. [More Information]
  • Lyons, G., Patel, V., Delic (nee Roue), N., Fok, S., Soon, L., Halliday, G., Gutkind, J. (2008). Snail up-regulates proinflammatory mediators and inhibits differentiation in oral keratinocytes. Cancer Research, 68(12), 4525-4530. [More Information]
  • Lyons, G., Lobo, E., Martorana, A., Myerscough, M. (2007). Clonal diversity in carcinomas: its implications for tumour progression and the contribution made to it by epithelial-mesenchymal transitions. Clinical and Experimental Metastasis, online DOI 10.1007/s10585-007-9134-2((printed version 2008 25 (6) 665-677)), 1-13. [More Information]
  • Min, D., Lyons, G., Jia, J., Lo, L., McLennan, S. (2006). 2-Methoxy-2,4-diphenyl-3(2H)-furanone-labeled gelatin zymography and reverse zymography: a rapid real-time method for quantification of matrix metalloproteinases-2 and -9 and tissue inhibitors of metalloproteinases. Electrophoresis, 27(2), 357-364. [More Information]
  • Lyons, G., Failes, T., Cullinane, C., Diakos, C., Yamamoto, N., Hambley, T. (2006). Studies of a Cobalt(III) Complex of the MMP Inhibitor Marimastat: A Potential Hypoxia-Activated Prodrug. Chemistry: A European Journal, DOI: 10.1002/chem.200601137. [More Information]
  • Failes, T., Diakos, C., Underwood, C., Hambley, T., Cullinane, C., Lyons, G. (2003). Can metal complexes serve as hypoxia activated prodrugs? Investigations of a Co(III) complex of the MMP inhibitor marimastat. Journal of Inorganic Biochemistry, 96(1), 128.
  • Underwood, C., Min, D., Lyons, G., Hambley, T. (2003). The interaction of metal ions and Marimastat with matrix metalloproteinase 9. Journal of Inorganic Biochemistry, 95(2-3), 165-170.
  • Min, D., Moore, A., Bain, M., Breit, S., Lyons, G. (2002). Activation of macrophage promatrix metalloproteinase-9 by lipopolysaccharide-associated proteinases. Journal of Immunology, 168(5), 2449-2455.
  • Zheng, G., Lyons, G. (2002). Cyclosporin A improves the selection of cells transfected with the puromycin acetyltransferase gene. BioTechniques: the journal of laboratory technology for bioresearch, 33(1), 32-36.
  • McLennan, S., Kelly, D., Cox, A., Cao, Z., Lyons, G., Yue, D., Gilbert, R. (2002). Decreased matrix degradation in diabetic nephropathy: effects of ACE inhibition on the expression and activities of matrix metalloproteinases. Diabetologia: clinical and experimental diabetes and metabolism, 45, 268-275. [More Information]

2014

  • Hassan, N., Painter, N., Howlett, C., Farrell, A., Di Girolamo, N., Lyons, G., Halliday, G. (2014). Brm Inhibits the Proliferative Response of Keratinocytes and Corneal Epithelial Cells to Ultraviolet Radiation-Induced Damage. PLoS One, 9(9), e107931. [More Information]

2013

  • Tian, X., Zhang, J., Tan, T., Lyons, G., Zhao, H., Niu, B., Lee, S., Tsatralis, T., Zhao, Y., Wang, Y., Cao, Q., Wang, C., Wang, Y., Lee, V., Zheng, G., Harris, D., et al (2013). Association of beta catenin with P-Smad3 but not LEF-1 dissociates in vitro profibrotic from anti-inflammatory effects of TGF- beta1. Journal of Cell Science, 126(1), 67-76. [More Information]
  • Jung, M., Lord, M., Cheng, B., Lyons, G., Alkhouri, H., Hughes, M., McCarthy, S., Iozzo, R., Whitelock, J. (2013). Mast Cells Produce Novel Shorter Forms of Perlecan That Contain Functional Endorepellin: A Role in Angiogenesis and Wound Healing. Journal of Biological Chemistry, 288(5), 3289-3304. [More Information]

2012

  • Chien, H., Fok, S., Raviraj, V., Lee, J., Cole, L., Lyons, G., Soon, L. (2012). Matrix Scaffolds in Cancer Cell Biology; Physiological Representation and Nanoscale Characterisation. In Raja Srirajaskanthan, Victor R. Preedy (Eds.), Nanomedicine and Cancer, (pp. 142-159). Enfield, New Hampshire: Science Publishers Inc.
  • Raviraj, V., Fok, S., Zhao, J., Chien, H., Lyons, G., Thompson, E., Soon, L. (2012). Regulation of ROCK1 via Notch1 during breast cancer cell migration into dense matrices. BMC Cell Biology, 13(5), 1-17. [More Information]
  • Halliday, G., Zhou, Y., Sou, P., Huang, X., Rana, S., Bugeja, M., Painter, N., Scolyer, R., Muchardt, C., Di Girolamo, N., Lyons, G. (2012). The absence of Brm exacerbates photocarcinogenesis. Experimental Dermatology, 21(8), 599-604. [More Information]

2011

  • Bock, V., Lyons, G., Huang, X., Jones, A., McDonald, L., Scolyer, R., Moloney, F., Barnetson, R., Halliday, G. (2011). BRM and BRG1 subunits of the SWI�?�SNF chromatin remodelling complex are downregulated upon progression of benign skin lesions into invasive tumours. British Journal of Dermatology, 164(6), 1221-1227. [More Information]
  • Farrell, A., Halliday, G., Lyons, G. (2011). Chromatin Structure Following UV-Induced DNA Damage-Repair or Death? International Journal of Molecular Sciences, 12(11), 8063-8085. [More Information]
  • Javeri, A., Lyons, G., Huang, X., Halliday, G. (2011). Downregulation of cockayne syndrome B protein reduces human 8-oxoguanine DNA glycostlase-1 expression and repair of ultraviolet radiation-induced 8-oxo-7,8-dihydro-2'-deoxyguanine. Cancer science, 102(9), 1651-1658. [More Information]
  • Pang, E., Delic (nee Roue), N., Hong, A., Zhang, M., Rose, B., Lyons, G. (2011). Radiosensitization of Oropharyngeal Squamous Cell Carcinoma Cells By Human Papillomavirus 16 Oncoprotein E6*I. International Journal of Radiation: Oncology - Biology - Physics, 79(3), 860-865. [More Information]

2010

  • Sou, P., Delic (nee Roue), N., Halliday, G., Lyons, G. (2010). Snail transcription factors in keratinocytes: enough to make your skin crawl. The International Journal of Biochemistry and Cell Biology, 42(12), 1940-1944. [More Information]

2009

  • Zheng, G., Lyons, J., Tan, T., Wang, Y., Hsu, T., Min, D., Succar, L., Rangan, G., Hu, M., Henderson, B., Alexander, S., Harris, D. (2009). Disruption of E-Cadherin by Matrix Metalloproteinase Directly Mediates Epithelial-Mesenchymal Transition Downstream of Transforming Growth Factor-{beta}1 in Renal Tubular Epithelial Cells. The American Journal of Pathology: cellular and molecular biology of disease, 175(2), 580-591. [More Information]
  • Min, D., Lyons, G., Bonner, J., Twigg, S., Yue, D., McLennan, S. (2009). Mesangial cell-derived factors alter monocyte activation and function through inflammatory pathways: possible pathogenic role in diabetic nephropathy. American Journal of Physiology: Renal Physiology (Print), 297(5), F1229-F1237. [More Information]
  • Halliday, G., Bock, V., Moloney, F., Lyons, G. (2009). SWI/SNF: A chromatin-remodelling complex with a role in carcinogenesis. The International Journal of Biochemistry and Cell Biology, 41(4), 725-728. [More Information]

2008

  • Moloney, F., Lyons, G., Bock, V., Huang, X., Bugeja, M., Halliday, G. (2008). Hotspot Mutation of Brahma in Non-Melanoma Skin Cancer. Journal of Investigative Dermatology, 129(4), 1012-1015. [More Information]
  • Halliday, G., Lyons, G. (2008). Inflammatory Doses of UV May Not Be Necessary for Skin Carcinogenesis. Photochemistry and Photobiology, 84(2), 272-283. [More Information]
  • Lyons, G., Patel, V., Delic (nee Roue), N., Fok, S., Soon, L., Halliday, G., Gutkind, J. (2008). Snail up-regulates proinflammatory mediators and inhibits differentiation in oral keratinocytes. Cancer Research, 68(12), 4525-4530. [More Information]

2007

  • Lyons, G., Lobo, E., Martorana, A., Myerscough, M. (2007). Clonal diversity in carcinomas: its implications for tumour progression and the contribution made to it by epithelial-mesenchymal transitions. Clinical and Experimental Metastasis, online DOI 10.1007/s10585-007-9134-2((printed version 2008 25 (6) 665-677)), 1-13. [More Information]

2006

  • Min, D., Lyons, G., Jia, J., Lo, L., McLennan, S. (2006). 2-Methoxy-2,4-diphenyl-3(2H)-furanone-labeled gelatin zymography and reverse zymography: a rapid real-time method for quantification of matrix metalloproteinases-2 and -9 and tissue inhibitors of metalloproteinases. Electrophoresis, 27(2), 357-364. [More Information]
  • Lyons, G., Failes, T., Cullinane, C., Diakos, C., Yamamoto, N., Hambley, T. (2006). Studies of a Cobalt(III) Complex of the MMP Inhibitor Marimastat: A Potential Hypoxia-Activated Prodrug. Chemistry: A European Journal, DOI: 10.1002/chem.200601137. [More Information]

2003

  • Failes, T., Diakos, C., Underwood, C., Hambley, T., Cullinane, C., Lyons, G. (2003). Can metal complexes serve as hypoxia activated prodrugs? Investigations of a Co(III) complex of the MMP inhibitor marimastat. Journal of Inorganic Biochemistry, 96(1), 128.
  • Underwood, C., Min, D., Lyons, G., Hambley, T. (2003). The interaction of metal ions and Marimastat with matrix metalloproteinase 9. Journal of Inorganic Biochemistry, 95(2-3), 165-170.

2002

  • Min, D., Moore, A., Bain, M., Breit, S., Lyons, G. (2002). Activation of macrophage promatrix metalloproteinase-9 by lipopolysaccharide-associated proteinases. Journal of Immunology, 168(5), 2449-2455.
  • Zheng, G., Lyons, G. (2002). Cyclosporin A improves the selection of cells transfected with the puromycin acetyltransferase gene. BioTechniques: the journal of laboratory technology for bioresearch, 33(1), 32-36.
  • McLennan, S., Kelly, D., Cox, A., Cao, Z., Lyons, G., Yue, D., Gilbert, R. (2002). Decreased matrix degradation in diabetic nephropathy: effects of ACE inhibition on the expression and activities of matrix metalloproteinases. Diabetologia: clinical and experimental diabetes and metabolism, 45, 268-275. [More Information]

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