Dr Holly Bolton

CINSW Early Career Development Fellow
School of Medical Sciences, Pathology

Member of the Charles Perkins Centre

Telephone 02 8627 6542

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Biographical details

Dr Holly Bolton is a research fellow at the Charles Perkins Centre. Dr Bolton completed her PhD at the Centenary Institute, Sydney in 2013 for her work examining the role of regulatory T cells in the regulation of dendritic cell co-stimulatory molecules. Her current research focuses on harnessing the protective effect of regulatory T cells to protect against graft-versus-host disease in a preclinical model of allogeneic stem cell transplantation.

Research interests

  • Harnessing regulatory T cells to improve T cell reconstitution following allogeneic stem cell transplantation. Allogeneic haematopoetic stem cell transplants are an effective treatment for leukemia due to the ability of donor-derived T cells to mediate a graft-versus-leukemia response. However, these same donor T cells may also attack host tissue, causing graft-versus-host disease (GVHD). Dr Bolton’s research focuses on how a subpopulation of T cells, regulatory T cells (Tregs) can be used to promote optimal T cell reconstitution after stem cell transplantation. Using a preclinical mouse model, we have shown that Treg infusion prior to transfer of allogeneic T cells can completely prevent development of GVHD, while promoting expansion of naïve T cells.
  • The role of CTLA-4 in Treg-mediated suppression. Previously we have shown that our Treg-infusion protocol is critically dependent upon CTLA-4-mediated down-regulation of CD80/CD86 co-stimulation on dendritic cells (DCs). We are currently investigating how CTLA-4 expressed by Treg cells mediates this effect, and if targeting CTLA-4-CD80/CD86 interactions can recapitulate the effect of Treg infusion in our animal models of disease.

Awards and honours

2017-2020: Cancer Institute NSW Early Career Researcher Fellowship

2016: Sydney Research Hub Research Excellence Award

2016: Centenary Institute Axel Ullrich Award for highest impact factor paper

2015: Finalist ASI Young Investigator Prize

2015: Bank of Queensland Women in Science Award

2011: Advanced Cytometry Facility NSW Flow Cytometry Prize

Selected grants

2018

  • Regulatory T cell therapy for prevention of graft versus host disease; Fazekas de St Groth B, Hart D, Bolton H, McGuire H; National Health and Medical Research Council (NHMRC)/Project Grants.

2017

  • Improving leukaemia therapy through prevention of graft-versus-host disease; Bolton H; Cancer Institute New South Wales/Early Career Fellowship.
  • Targeting dendritic cell costimulation to prevent graft-versus-host disease; Bolton H; Sydney Medical School/Kickstart Grant.

Selected publications

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Journals

  • Guy, T., Terry, A., Bolton, H., Hancock, D., Zhu, E., Brink, R., McGuire, H., Shklovskaya, E., Fazekas de St Groth, B. (2016). Collaboration between tumor-specific CD4+ T cells and B cells in anti-cancer immunity. Oncotarget, 7(21), 30211-30229. [More Information]
  • Guy, T., Terry, A., Bolton, H., Hancock, D., Shklovskaya, E., Fazekas de St Groth, B. (2016). Pro- and anti-tumour effects of B cells and antibodies in cancer: a comparison of clinical studies and preclinical models. Cancer Immunology, Immunotherapy, 65(8), 885-896. [More Information]
  • Bolton, H., Fazekas de St Groth, B. (2016). Reconstitution of Lymphopaenic Mice with Regulatory and Conventional T Cell Subsets. Bio-protocol, 6(10), 1-9.
  • Shklovskaya, E., Terry, A., Guy, T., Buckley, A., Bolton, H., Zhu, E., Holst, J., Fazekas de St Groth, B. (2016). Tumour-specific CD4 T cells eradicate melanoma via indirect recognition of tumour-derived antigen. Immunology and Cell Biology, 94(6), 593-603. [More Information]
  • Roediger, B., Kyle, R., Tay, S., Mitchell, A., Bolton, H., Guy, T., Tan, S., Forbes-Blom, E., Tong, P., Koller, Y., Shklovskaya, E., Fazekas de St Groth, B., Weninger, W., et al (2015). IL-2 is a critical regulator of group 2 innate lymphoid cell function during pulmonary inflammation. Journal of Allergy and Clinical Immunology, 136(6), 1653-1663e7. [More Information]
  • Bolton, H., Zhu, E., Terry, A., Guy, T., Koh, W., Tan, S., Power, C., Bertolino, P., Lahl, K., Sparwasser, T., Shklovskaya, E., Fazekas de St Groth, B. (2015). Selective Treg reconstitution during lymphopenia normalizes DC costimulation and prevents graft-versus-host disease. Journal of Clinical Investigation, 125(9), 3627-3641. [More Information]
  • Bolton, H., Roediger, B., Fazekas de St Groth, B. (2015). The effects of IL-2 and Treg cells on dendritic cell homeostasis are mediated indirectly via activation of conventional T cells. European Journal of Immunology, 45(4), 1141-1147. [More Information]

2016

  • Guy, T., Terry, A., Bolton, H., Hancock, D., Zhu, E., Brink, R., McGuire, H., Shklovskaya, E., Fazekas de St Groth, B. (2016). Collaboration between tumor-specific CD4+ T cells and B cells in anti-cancer immunity. Oncotarget, 7(21), 30211-30229. [More Information]
  • Guy, T., Terry, A., Bolton, H., Hancock, D., Shklovskaya, E., Fazekas de St Groth, B. (2016). Pro- and anti-tumour effects of B cells and antibodies in cancer: a comparison of clinical studies and preclinical models. Cancer Immunology, Immunotherapy, 65(8), 885-896. [More Information]
  • Bolton, H., Fazekas de St Groth, B. (2016). Reconstitution of Lymphopaenic Mice with Regulatory and Conventional T Cell Subsets. Bio-protocol, 6(10), 1-9.
  • Shklovskaya, E., Terry, A., Guy, T., Buckley, A., Bolton, H., Zhu, E., Holst, J., Fazekas de St Groth, B. (2016). Tumour-specific CD4 T cells eradicate melanoma via indirect recognition of tumour-derived antigen. Immunology and Cell Biology, 94(6), 593-603. [More Information]

2015

  • Roediger, B., Kyle, R., Tay, S., Mitchell, A., Bolton, H., Guy, T., Tan, S., Forbes-Blom, E., Tong, P., Koller, Y., Shklovskaya, E., Fazekas de St Groth, B., Weninger, W., et al (2015). IL-2 is a critical regulator of group 2 innate lymphoid cell function during pulmonary inflammation. Journal of Allergy and Clinical Immunology, 136(6), 1653-1663e7. [More Information]
  • Bolton, H., Zhu, E., Terry, A., Guy, T., Koh, W., Tan, S., Power, C., Bertolino, P., Lahl, K., Sparwasser, T., Shklovskaya, E., Fazekas de St Groth, B. (2015). Selective Treg reconstitution during lymphopenia normalizes DC costimulation and prevents graft-versus-host disease. Journal of Clinical Investigation, 125(9), 3627-3641. [More Information]
  • Bolton, H., Roediger, B., Fazekas de St Groth, B. (2015). The effects of IL-2 and Treg cells on dendritic cell homeostasis are mediated indirectly via activation of conventional T cells. European Journal of Immunology, 45(4), 1141-1147. [More Information]

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