Professor Ian Alexander

Professor in Paediatrics & Molecular Medicine
Disciplines of Paediatrics and Child Health and Genetic Medicine, The Children's Hospital at Westmead

Telephone +61 2 9845 3071
Fax +61 2 9845 1317

Map

Research interests

Prof Alexander's specific expertise and interests include virus-mediated gene transfer with a focus on target organs including the liver and bone marrow, both of which have immense promise as target organs for the treatment of genetic disease in children. His team became the first in Australia to treat a genetic disease (SCID-X1) by gene therapy and are recognised leaders in the establishment of this exciting field in Australia.

Selected grants

2015

  • Functional restoration of OTC deficient primary human hepatocytes in a xenograft model using an AAV vector uniquely configured for impending clinical trial use.; Alexander I, Gissen P; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Treatment of genetic liver disease by homologous recombination in vivo, coupled with a pharmoco-genetic strategy for selective expansion of genetically repaired hepatocytes; Alexander I, Grompe M, Nagy A; National Health and Medical Research Council (NHMRC)/Project Grants.

2014

  • WAVE 20/50 Bioreactor and C5 Cell Disruptor; Chircop (nee Fabbro) M, Kizana E, Robinson P, Diefenbach R, Bryan T, Gottlieb D, Micklethwaite K, Alexander I; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Development of a gene therapy approach for X-linked agammaglobulinaemia, the most prevalent primary immune deficiency disorder in man.; Alexander I, Thrasher A; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Development of a novel hybrid rAAV/transposon gene delivery system for life-long correction of metabolic liver disease in infants and children; Alexander I, Nagy A, Carpenter K; National Health and Medical Research Council (NHMRC)/Project Grants.
  • NanoString nCounter Platform; deFazio A, Alexander I, Booth D, Byrne J, Christodoulou J, Clarke C, Cunningham A, George J, Graham J, Harman A, Liddle C, Mann G; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2013

  • Fluidigm BioMark HD - HX; Alexander I, Jamieson R, Chircop (nee Fabbro) M, Byrne J, Catchpoole D, Reddel R, Tam P, Robinson P, Bryan T, Diefenbach R; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2012

  • Analysis and manipulation of the genome-wide integration signatures of gamma-retroviral and lentiviral vectors in human haematopoietic stem cells; Alexander I, Ranganathan S, Cavazzana-Calvo M; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Cellular therapy for genetic liver disease exploiting induced pluripotent stem cells and liver progenitor cells; Alexander I, Tam P, Russell D, Yeoh G; National Health and Medical Research Council (NHMRC)/Project Grants.

2011

  • ION TORRENT PERSONAL GENOME MACHINE (PGM).; North K, Alexander I, Alexander S, Christodoulou J, Catchpoole D, Clarke N; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • In vivo gene transfer and phenotype correction of normal and urea-cycle deficient primary human hepatocytes in chimeric mouse-human livers: Towards gene therapy for metabolic liver disease; Alexander I, Braet F, Christodoulou J, Kuchel P, Carpenter K; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Novel therapeutic interventions for the orthopaedic complications of Neurofibromatosis type 1 (NF1); Little D, Alexander I, Schindeler A; National Health and Medical Research Council (NHMRC)/Project Grants.

2010

  • Gene therapy, stress haematopoiesis and the risk of malignancy; Alexander I, Kuchel P, Rasko J, Alexander S, McCormack M, Thrasher A, Ginn S; National Health and Medical Research Council (NHMRC)/Project Grants.

2008

  • Induction of antigen-specific humoral tolerance by rAAV-mediated delivery of CTLA4-Ig-antigen fusion; Alexander I, Bishop A; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Modelling the loss of NF1 heterozygosity in congenital pseudarthrosis of the tibia (CPT); Little D, Baldock P, Schindeler A, Alexander I; National Health and Medical Research Council (NHMRC)/Project Grants.

2007

  • Role of tryptophan metabolism in liver transplant tolerance and rejection; Bishop A, Hall B, McCaughan G, Sharland A, Alexander I, Earl J, Hall B; National Health and Medical Research Council (NHMRC)/Project Grants.

Selected publications

Download citations: PDF RTF Endnote

Book Chapters

  • De Greef, E., Christodoulou, J., Alexander, I., Shun, A., O'Loughlin, E., Thorburn, D., Jermyn, V., Stormon, M. (2012). Mitochondrial Respiratory Chain Hepatopathies: Role of Liver Transplantation. A Case Series of Five Patients. In Society for the Study of Inborn Errors of Metabolism (Eds.), JIMD Reports - Case and Research Reports, 2012/1, (pp. 5-12). London: Springer Science + Business Media.
  • Fleming, J., Ginn, S., Alexander, I. (2003). Dorsal root ganglia sensory neurons. In Federico (Eds.), Lentivirus Gene Engineering Protocols, (pp. 155-167). New Jersey: Humana Press.

Journals

  • Lisowski, L., Dane, A., Chu, K., Zhang, Y., Cunningham, S., Wilson, E., Nygaard, S., Grompe, M., Alexander, I., Kay, M. (2014). Selection and evaluation of clinically relevant AAV variants in a xenograft liver model. Nature, 506(7488), 382-386. [More Information]
  • Kojima, Y., Kaufman-Francis, K., Studdert, J., Steiner, K., Power, M., Loebel, D., Jones, V., Hor, A., de Alencastro, G., Logan, G., Alexander, I., Pickett, H., Tam, P., et al (2014). The Transcriptional and Functional Properties of Mouse Epiblast Stem Cells Resemble the Anterior Primitive Streak. Cell Stem Cell, 14(1), 107-120. [More Information]
  • Cunningham, S., Kok, C., Spinoulas, A., Carpenter, K., Alexander, I. (2013). AAV-encoded OTC activity persisting to adulthood following delivery to newborn spfash mice is insufficient to prevent shRNA-induced hyperammonaemia. Gene Therapy, 20(12), 1184-1187. [More Information]
  • Kok, C., Cunningham, S., Carpenter, K., Dane, A., Siew, S., Logan, G., Kuchel, P., Alexander, I. (2013). Adeno-associated Virus-mediated Rescue of Neonatal Lethality in Argininosuccinate Synthetase-deficient Mice. Molecular Therapy, 21(10), 1823-1831. [More Information]
  • Dane, A., Wowro, S., Cunningham, S., Alexander, I. (2013). Comparison of gene transfer to the murine liver following intraperitoneal and intraportal delivery of hepatotropic AAV pseudo-serotypes. Gene Therapy, 20(4), 460-464. [More Information]
  • Ginn, S., Alexander, I., Edelstein, M., Abedi, M., Wixon, J. (2013). Gene therapy clinical trials worldwide to 2012 - an update. Journal of Gene Medicine, 15(2), 65-77. [More Information]
  • Cunningham, E., Tay, S., Wang, C., Rtshiladze, M., Wang, Z., McGuffog, C., Cubitt, J., McCaughan, G., Alexander, I., Bertolino, P., Sharland, A., Bowen, D., Bishop, A. (2013). Gene therapy for tolerance: high-level expression of donor major histocompatibility complex in the liver overcomes naive and memory alloresponses to skin grafts. Transplantation, 95(1), 70-77. [More Information]
  • Deakin, C., Alexander, I., Hooker, C., Kerridge, I. (2013). Gene Therapy Researchers' Assessments Of Risks And Perceptions Of Risk Acceptability In Clinical Trials. Molecular Therapy, 21(4), 806-815. [More Information]
  • Gerace, D., Ren, B., Hawthorne, W., Byrne, M., Phillips, P., O'Brien, B., Nassif, N., Alexander, I., Simpson, A. (2013). Pancreatic Transdifferentiation in Porcine Liver Following Lentiviral Delivery of Human Furin–Cleavable Insulin. Transplantation Proceedings, 45(5), 1869-1874. [More Information]
  • El-Hoss, J., Sullivan, K., Cheng, T., Yu, N., Bobyn, J., Peacock, L., Mikulec, K., Baldock, P., Alexander, I., Schindeler, A., Little, D. (2012). A murine model of neurofibromatosis type 1 tibial pseudarthrosis featuring proliferative fibrous tissue and osteoclast-like cells. Journal of Bone and Mineral Research, 27(1), 68-78. [More Information]
  • Logan, G., Alexander, I. (2012). Adeno-associated virus vectors: immunobiology and potential use for immune modulation. Current Gene Therapy, 12(4), 333-343. [More Information]
  • Alexander, I., Kok, C., Dane, A., Cunningham, S. (2012). Gene therapy for metabolic disorders: an overview with a focus on urea cycle disorders. Journal of Inherited Metabolic Disease (JIMD), 35(4), 641-645. [More Information]
  • Ginn, S., Alexander, I. (2012). Gene therapy: Progress in childhood disease. Journal of Paediatrics and Child Health, 48(6), 466-471. [More Information]
  • Chung, H., Lin, R., Logan, G., Alexander, I., Sachdev, P., Sidhu, K. (2012). Human Induced Pluripotent Stem Cells Derived Under Feeder-Free Conditions Display Unique Cell Cycle and DNA Replication Gene Profiles. Stem Cells and Development, 21(2), 206-216. [More Information]
  • De Greef, E., Christodoulou, J., Alexander, I., Shun, A., O'Loughlin, E., Thorburn, D., Jermyn, V., Stormon, M. (2012). Mitochondrial Respiratory Chain Hepatopathies: Role of Liver Transplantation. A Case Series of Five Patients. JIMD Reports - Case and Research Reports, 4, 5-12.
  • Michot, C., Hubert, L., Romero, N., Gouda, A., Mamoune, A., Mathew, S., Kirk, E., Viollet, L., Rahman, S., Bekri, S., Alexander, I., et al (2012). Study of LPIN1, LPIN2 and LPIN3 in rhabdomyolysis and exercise-induced myalgia. Journal of Inherited Metabolic Disease (JIMD), 35(6), 1119-1128. [More Information]
  • Both, G., Alexander, I., Fletcher, S., Nicolson, T., Rasko, J., Wilton, S., Symonds, G. (2011). Gene therapy: therapeutic applications and relevance to pathology. Pathology, 43(6), 642-656. [More Information]
  • Cunningham, S., Kok, C., Dane, A., Carpenter, K., Kizana, E., Kuchel, P., Alexander, I. (2011). Induction and Prevention of Severe Hyperammonemia in the spf(ash) Mouse Model of Ornithine Transcarbamylase Deficiency Using shRNA and rAAV-mediated Gene Delivery. Molecular Therapy, 19(5), 854-859. [More Information]
  • Logan, G., Wang, L., Zheng, M., Coppel, R., Alexander, I. (2010). Antigen fusion with C3d(3) augments or inhibits humoral immunity to AAV genetic vaccines in a transgene-dependent manner. Immunology and Cell Biology, 88(2), 228-232. [More Information]
  • Sharland, A., Logan, G., Bishop, A., Alexander, I. (2010). Liver-directed gene expression using recombinant AAV 2/8 vectors -- a tolerogenic strategy for gene delivery? Discovery Medicine, 9(49), 519-527. [More Information]
  • Ginn, S., Liao, S., Dane, A., Hu, M., Hyman, J., Finnie, J., Zheng, M., Cavazzana-Calvo, M., Alexander, S., Thrasher, A., Alexander, I. (2010). Lymphomagenesis in SCID-X1 Mice Following Lentivirus-mediated Phenotype Correction Independent of Insertional Mutagenesis and gammac Overexpression. Molecular Therapy, 18(5), 965-976. [More Information]
  • Deakin, C., Alexander, I., Kerridge, I. (2010). The ethics of gene therapy: Balancing the risks. Current Opinion in Molecular Therapeutics, 12(5), 578-585. [More Information]
  • Cunningham, S., Spinoulas, A., Carpenter, K., Wilcken, B., Kuchel, P., Alexander, I. (2009). AAV2/8-mediated Correction of OTC Deficiency Is Robust in Adult but Not Neonatal Spf(ash) Mice. Molecular Therapy, 17(8), 1340-1346. [More Information]
  • Deakin, C., Alexander, I., Kerridge, I. (2009). Accepting risk in clinical research: is the gene therapy field becoming too risk-averse? Molecular Therapy, 17(11), 1842-1848. [More Information]
  • Logan, G., Wang, L., Zheng, M., Ginn, S., Coppel, R., Alexander, I. (2009). Antigen-specific humoral tolerance or immune augmentation induced by intramuscular delivery of adeno-associated viruses encoding CTLA4-Ig-antigen fusion molecules. Gene Therapy (Basingstoke), 16(2), 200-210. [More Information]
  • Laurence, J., McCaughan, G., Logan, G., Alexander, I., Bishop, A., Sharland, A. (2009). Gene therapy in transplantation. Transplantation Reviews, 23(3), 159-170. [More Information]
  • Cunningham, S., Zoccai, G., Spinoulas, A., Carpenter, K., Alexander, I. (2009). In vivo assessment of mutations in OTC for dominant-negative effects following rAAV2/8-mediated gene delivery to the mouse liver. Gene Therapy, 16(6), 820-823. [More Information]
  • Liu, R., Ginn, S., Lek, M., North, K., Alexander, I., Little, D., Schindeler, A. (2009). Myoblast sensitivity and fibroblast insensitivity to osteogenic conversion by BMP-2 correlates with the expression of Bmpr-1 a. BMC Musculoskeletal Disorders, 10, 51-1-51-12. [More Information]
  • Laurence, J., Wang, C., Zheng, M., Cunningham, S., Earl, J., Tay, S., Allen, R., McCaughan, G., Alexander, I., Bishop, A., Sharland, A. (2009). Overexpression of indoleamine dioxygenase in rat liver allografts using a high-efficiency adeno-associated virus vector does not prevent acute rejection. Liver Transplantation, 15(2), 233-241. [More Information]
  • Dane, A., Cunningham, S., Graf, N., Alexander, I. (2009). Sexually Dimorphic Patterns of Episomal rAAV Genome Persistence in the Adult Mouse Liver and Correlation With Hepatocellular Proliferation. Molecular Therapy, 17(9), 1548-1554. [More Information]
  • Curtin, J., Dane, A., Swanson, A., Alexander, I., Ginn, S. (2008). Bidirectional promoter interference between two widely used internal heterologous promoters in a late-generation lentiviral construct. Gene Therapy (Basingstoke), 15(5), 384-390. [More Information]
  • Baoutina, A., Alexander, I., Rasko, J., Emslie, K. (2008). Developing strategies for detection of gene doping. Journal of Gene Medicine, 10, 3-20. [More Information]
  • Cunningham, S., Dane, A., Spinoulas, A., Alexander, I. (2008). Gene delivery to the juvenile mouse liver using AAV2/8 vectors. Molecular Therapy, 16(6), 1081-1088. [More Information]
  • Alexander, I., Cunningham, S., Logan, G., Christodoulou, J. (2008). Potential of AAV vectors in the treatment of metabolic disease. Gene Therapy, 15(11), 831-839. [More Information]
  • Lee, V., Wang, Y., Wang, Y., Zheng, D., Polhill, T., Cao, Q., Wu, H., Alexander, I., Alexander, S., Harris, D. (2008). Regulatory immune cells in kidney disease. American Journal of Physiology: Renal Physiology (Print), 295(2), F335-F342. [More Information]
  • Cooper, S., Kizana, E., Yates, J., Lo, H., Yang, N., Wu, Z., Alexander, I., North, K. (2007). Dystrophinopathy carrier determination and detection of protein deficiencies in muscular dystrophy using lentiviral MyoD-forced myogenesis. Neuromuscular Disorders, 17(4), 276-284. [More Information]
  • Kizana, E., Chang, C., Cingolani, E., Sekar, R., Ramirez-Correa, G., Abraham, M., Ginn, S., Tung, L., Alexander, I., Marban, E. (2007). Gene transfer of connexin43 mutants attenuates coupling in cardiomyocytes: novel basis for modulation of cardiac conduction by gene therapy. Circulation Research. [More Information]
  • Yu, Z., McKay, K., Van Asperen, P., Zheng, M., Fleming, J., Ginn, S., Kizana, E., Latham, M., Feneley, M., Kirkland, P., Lumbers, E., Alexander, I., et al (2007). Lentivirus vector-mediated gene transfer to the developing bronchiolar airway epithelium in the fetal lamb. Journal of Gene Medicine, 9(6), 429-439. [More Information]
  • Smyth, C., Ginn, S., Deakin, C., Logan, G., Alexander, I. (2007). Limiting {gamma}c expression differentially affects signaling via the interleukin (IL)-7 and IL-15 receptors. Blood, 110(1), 91-98. [More Information]
  • Ganesan, P., Alexander, S., Watson, D., Logan, G., Zhang, G., Alexander, I. (2007). Robust anti-tumor immunity and memory in Rag-1-deficient mice following adoptive transfer of cytokine-primed splenocytes and tumor CD80 expression. Cancer Immunology, Immunotherapy: other biological response modifications, 56(12), 1955-1965. [More Information]
  • Deichmann, A., Hacein-Bey-Abina, S., Schmidt, M., Garrigue, A., Brugman, M., Hu, J., Glimm, H., Gyapay, G., Prum, B., et, A., Alexander, I. (2007). Vector integration is nonrandom and clustered and influences the fate of lymphopoiesis in SCID-X1 gene therapy. Journal of Clinical Investigation, 117(8), 2225-2232. [More Information]
  • Logan, G., Wang, L., Zheng, M., Cunningham, S., Coppel, R., Alexander, I. (2006). AAV vectors encoding malarial antigens stimulate antigen-specific immunity but do not protect from parasite infection. Vaccine, 25(6), 1014-1022. [More Information]
  • Kramer, B., Lemckert, F., Alexander, I., Gunning, P., McCowage, G. (2006). Characterisation of a P140K mutant O(6)-methylguanine-DNA-methyltransferase (MGMT)-expressing transgenic mouse line with drug-selectable bone marrow. Journal of Gene Medicine, 8(9), 1071-1085. [More Information]
  • Kizana, E., Ginn, S., Smyth, C., Boyd, A., Thomas, S., Allen, D., Ross, D., Alexander, I. (2006). Fibroblasts modulate cardiomyocyte excitability: implications for cardiac gene therapy. Gene Therapy (Basingstoke), 13(22), 1611-1615. [More Information]
  • Wang, Y., Zhang, G., Wang, Y., Hu, M., Wu, H., Watson, D., Hori, S., Alexander, I., Harris, D., Alexander, S. (2006). Foxp3-transduced polyclonal regulatory T cells protect against chronic renal injury from adriamycin. Journal of the American Society of Nephrology, 17(3), 697-706. [More Information]
  • Trent, R., Alexander, I. (2006). Gene therapy in sport. British Journal of Sports Medicine, 40(1), 4-5. [More Information]
  • Hu, M., Kramer, B., Zhang, G., McCowage, G., Alexander, I., Gunning, P., Alexander, S. (2006). Long term skin allograft survival in mice with MGMT chimeric bone marrow engraftment. Immunology and Cell Biology, 84 (Suppl.)(3), A29-A29.
  • Alexander, I., Logan, G., Logan, G., Boadle, R., Rowe, P., Smythe, J. (2005). Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy. International Immunology, 17(2), 123-132. [More Information]
  • Smyth, C., Logan, G., Boadle, R., Rowe, P., Smythe, J., Alexander, I. (2005). Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy. International Immunology, 17(2), 123-132.
  • Kizana, E., Ginn, S., Allen, D., Ross, D., Alexander, I. (2005). Fibroblasts can be genetically modified to produce excitable cells capable of electrical coupling. Circulation, 111(4), 394-398. [More Information]
  • Kizana, E., Ginn, S., Smyth, C., Thomas, S., Allen, D., Ross, D., Alexander, I. (2005). Fibroblasts Modulate Cardiomyocyte Excitability: Insights from Fibroblasts with Genetically Altered Connexin43 Expression. Journal of Gene Medicine, 7(point P8 on page 1127), 1127-1127.
  • Fleming, J., Spinoulas, A., Zheng, M., Cunningham, S., Ginn, S., McQuilty, R., Rowe, P., Alexander, I. (2005). Partial correction of sensitivity to oxidant stress in Friedreich ataxia patient fibroblasts by frataxin-encoding adeno-associated virus and lentivirus vectors. Human Gene Therapy, 16(8), 947-956. [More Information]
  • Mitchell, J., Wilcken, B., Alexander, I., Ellaway, C., O'Grady, H., Wiley, V., Earl, J., Christodoulou, J. (2005). Tetrahydrobiopterin-responsive phenylketonuria: The New South Wales experience. Molecular Genetics and Metabolism, 86(Suppl 1), S81-S85. [More Information]
  • Ginn, S., Curtin, J., Kramer, B., Smyth, C., Wong, M., Kakakios, A., McCowage, G., Watson, D., Alexander, S., Latham, M., Rowe, P., Alexander, I., et al (2005). Treatment of an infant with X-linked severe combined immunodeficiency (SCID-X1) by gene therapy in Australia. Medical Journal of Australia, 182(9), 458-463. [More Information]
  • Ginn, S., Smyth, C., Wong, M., Bennetts, B., Rowe, P., Alexander, I. (2004). A Novel Splice-Site Mutation In The Common Gamma Chain (Gammac) Gene Il2Rg Results In X-Linked Severe Combined Immunodeficiency With An Atypical Nk+ Phenotype. Human Mutation, 23(5), 522-523.
  • Allen, D., Hardeman, E., North, K., Alexander, I., Cooper, S., Maxwell, A., Kizana, E., Ghoddusi, M. (2004). C2C12 Co-Culture On A Fibroblast Substratum Enables Sustained Survival Of Contractile, Highly Differentiated Myotubes With Peripheral Nuclei And Adult Fast Myosin Expression. Cell Motility and the Cytoskeleton, 58(3), 200-211. [More Information]
  • Logan, G., Spinoulas, A., Alexander, S., Smythe, J., Alexander, I. (2004). CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection. Immunology and Cell Biology, 82(2), 132-135.
  • Trent, R., Alexander, I. (2004). Gene Therapy: Applications And Progress Towards The Clinic. Internal Medicine Journal, 34(11), 621-625.
  • Alexander, I., Kizana, E. (2003). Cardiac gene therapy: therapeutic potential and current progress. Current Gene Therapy: the international journal for in-depth reviews on gene therapy, 3(5), 418-451.
  • Ginn, S., Fleming, J., Curtin, J., Alexander, I. (2003). Gene therapy: a roller-coaster ride. Today's Life Science (Australia), 15(3), 26-29.
  • Hacein-Bey-Abina, S., Von Kalle, C., Schmidt, M., McCormack, M., Wulffraat, N., Leboulch, P., Lim, A., Osborne, C., Pawliuk, R., Morillon, E., Alexander, I., et al (2003). LMO2-associated clonal T Cell proliferation in two patients after gene therapy for SCID-X1. Science, 302(5644), 415-419.
  • Ginn, S., Fleming, J., Rowe, P., Alexander, I. (2003). Promoter interference mediated by the U3 Region in early-generation HIV-1-Derived Lentivirus Vectors can influence detection of transgene expression in a cell-type and species-specific manner. Human Gene Therapy, 14(12), 1127-1137.
  • Alexander, I. (2002). Australasian Gene Therapy Society kicks off. Journal of Gene Medicine, , 4-4.
  • Logan, G., Smyth, C., Earl, J., Rowe, P., Zaikina, I., Smythe, J., Alexander, I. (2002). HeLa cells cocultured with periopheral blood lymphocytes acquire an immuno-inhibitory phenotype through up-regulation of indoleamine 2,3-dioxygenase activity. Immunology, 105(4), 478-487.

Conferences

  • Hu, M., Kramer, B., Zhang, G., McCowage, G., Alexander, I., Gunning, P., Alexander, S. (2006). Long term skin allograft survival in mice with MGMT chimeric bone marrow engraftment. 24th Annual Scientific Meeting of the Transplantation Society of Australia and New Zealand (2006), Australia: Nature Publishing Group.

2014

  • Lisowski, L., Dane, A., Chu, K., Zhang, Y., Cunningham, S., Wilson, E., Nygaard, S., Grompe, M., Alexander, I., Kay, M. (2014). Selection and evaluation of clinically relevant AAV variants in a xenograft liver model. Nature, 506(7488), 382-386. [More Information]
  • Kojima, Y., Kaufman-Francis, K., Studdert, J., Steiner, K., Power, M., Loebel, D., Jones, V., Hor, A., de Alencastro, G., Logan, G., Alexander, I., Pickett, H., Tam, P., et al (2014). The Transcriptional and Functional Properties of Mouse Epiblast Stem Cells Resemble the Anterior Primitive Streak. Cell Stem Cell, 14(1), 107-120. [More Information]

2013

  • Cunningham, S., Kok, C., Spinoulas, A., Carpenter, K., Alexander, I. (2013). AAV-encoded OTC activity persisting to adulthood following delivery to newborn spfash mice is insufficient to prevent shRNA-induced hyperammonaemia. Gene Therapy, 20(12), 1184-1187. [More Information]
  • Kok, C., Cunningham, S., Carpenter, K., Dane, A., Siew, S., Logan, G., Kuchel, P., Alexander, I. (2013). Adeno-associated Virus-mediated Rescue of Neonatal Lethality in Argininosuccinate Synthetase-deficient Mice. Molecular Therapy, 21(10), 1823-1831. [More Information]
  • Dane, A., Wowro, S., Cunningham, S., Alexander, I. (2013). Comparison of gene transfer to the murine liver following intraperitoneal and intraportal delivery of hepatotropic AAV pseudo-serotypes. Gene Therapy, 20(4), 460-464. [More Information]
  • Ginn, S., Alexander, I., Edelstein, M., Abedi, M., Wixon, J. (2013). Gene therapy clinical trials worldwide to 2012 - an update. Journal of Gene Medicine, 15(2), 65-77. [More Information]
  • Cunningham, E., Tay, S., Wang, C., Rtshiladze, M., Wang, Z., McGuffog, C., Cubitt, J., McCaughan, G., Alexander, I., Bertolino, P., Sharland, A., Bowen, D., Bishop, A. (2013). Gene therapy for tolerance: high-level expression of donor major histocompatibility complex in the liver overcomes naive and memory alloresponses to skin grafts. Transplantation, 95(1), 70-77. [More Information]
  • Deakin, C., Alexander, I., Hooker, C., Kerridge, I. (2013). Gene Therapy Researchers' Assessments Of Risks And Perceptions Of Risk Acceptability In Clinical Trials. Molecular Therapy, 21(4), 806-815. [More Information]
  • Gerace, D., Ren, B., Hawthorne, W., Byrne, M., Phillips, P., O'Brien, B., Nassif, N., Alexander, I., Simpson, A. (2013). Pancreatic Transdifferentiation in Porcine Liver Following Lentiviral Delivery of Human Furin–Cleavable Insulin. Transplantation Proceedings, 45(5), 1869-1874. [More Information]

2012

  • El-Hoss, J., Sullivan, K., Cheng, T., Yu, N., Bobyn, J., Peacock, L., Mikulec, K., Baldock, P., Alexander, I., Schindeler, A., Little, D. (2012). A murine model of neurofibromatosis type 1 tibial pseudarthrosis featuring proliferative fibrous tissue and osteoclast-like cells. Journal of Bone and Mineral Research, 27(1), 68-78. [More Information]
  • Logan, G., Alexander, I. (2012). Adeno-associated virus vectors: immunobiology and potential use for immune modulation. Current Gene Therapy, 12(4), 333-343. [More Information]
  • Alexander, I., Kok, C., Dane, A., Cunningham, S. (2012). Gene therapy for metabolic disorders: an overview with a focus on urea cycle disorders. Journal of Inherited Metabolic Disease (JIMD), 35(4), 641-645. [More Information]
  • Ginn, S., Alexander, I. (2012). Gene therapy: Progress in childhood disease. Journal of Paediatrics and Child Health, 48(6), 466-471. [More Information]
  • Chung, H., Lin, R., Logan, G., Alexander, I., Sachdev, P., Sidhu, K. (2012). Human Induced Pluripotent Stem Cells Derived Under Feeder-Free Conditions Display Unique Cell Cycle and DNA Replication Gene Profiles. Stem Cells and Development, 21(2), 206-216. [More Information]
  • De Greef, E., Christodoulou, J., Alexander, I., Shun, A., O'Loughlin, E., Thorburn, D., Jermyn, V., Stormon, M. (2012). Mitochondrial Respiratory Chain Hepatopathies: Role of Liver Transplantation. A Case Series of Five Patients. JIMD Reports - Case and Research Reports, 4, 5-12.
  • De Greef, E., Christodoulou, J., Alexander, I., Shun, A., O'Loughlin, E., Thorburn, D., Jermyn, V., Stormon, M. (2012). Mitochondrial Respiratory Chain Hepatopathies: Role of Liver Transplantation. A Case Series of Five Patients. In Society for the Study of Inborn Errors of Metabolism (Eds.), JIMD Reports - Case and Research Reports, 2012/1, (pp. 5-12). London: Springer Science + Business Media.
  • Michot, C., Hubert, L., Romero, N., Gouda, A., Mamoune, A., Mathew, S., Kirk, E., Viollet, L., Rahman, S., Bekri, S., Alexander, I., et al (2012). Study of LPIN1, LPIN2 and LPIN3 in rhabdomyolysis and exercise-induced myalgia. Journal of Inherited Metabolic Disease (JIMD), 35(6), 1119-1128. [More Information]

2011

  • Both, G., Alexander, I., Fletcher, S., Nicolson, T., Rasko, J., Wilton, S., Symonds, G. (2011). Gene therapy: therapeutic applications and relevance to pathology. Pathology, 43(6), 642-656. [More Information]
  • Cunningham, S., Kok, C., Dane, A., Carpenter, K., Kizana, E., Kuchel, P., Alexander, I. (2011). Induction and Prevention of Severe Hyperammonemia in the spf(ash) Mouse Model of Ornithine Transcarbamylase Deficiency Using shRNA and rAAV-mediated Gene Delivery. Molecular Therapy, 19(5), 854-859. [More Information]

2010

  • Logan, G., Wang, L., Zheng, M., Coppel, R., Alexander, I. (2010). Antigen fusion with C3d(3) augments or inhibits humoral immunity to AAV genetic vaccines in a transgene-dependent manner. Immunology and Cell Biology, 88(2), 228-232. [More Information]
  • Sharland, A., Logan, G., Bishop, A., Alexander, I. (2010). Liver-directed gene expression using recombinant AAV 2/8 vectors -- a tolerogenic strategy for gene delivery? Discovery Medicine, 9(49), 519-527. [More Information]
  • Ginn, S., Liao, S., Dane, A., Hu, M., Hyman, J., Finnie, J., Zheng, M., Cavazzana-Calvo, M., Alexander, S., Thrasher, A., Alexander, I. (2010). Lymphomagenesis in SCID-X1 Mice Following Lentivirus-mediated Phenotype Correction Independent of Insertional Mutagenesis and gammac Overexpression. Molecular Therapy, 18(5), 965-976. [More Information]
  • Deakin, C., Alexander, I., Kerridge, I. (2010). The ethics of gene therapy: Balancing the risks. Current Opinion in Molecular Therapeutics, 12(5), 578-585. [More Information]

2009

  • Cunningham, S., Spinoulas, A., Carpenter, K., Wilcken, B., Kuchel, P., Alexander, I. (2009). AAV2/8-mediated Correction of OTC Deficiency Is Robust in Adult but Not Neonatal Spf(ash) Mice. Molecular Therapy, 17(8), 1340-1346. [More Information]
  • Deakin, C., Alexander, I., Kerridge, I. (2009). Accepting risk in clinical research: is the gene therapy field becoming too risk-averse? Molecular Therapy, 17(11), 1842-1848. [More Information]
  • Logan, G., Wang, L., Zheng, M., Ginn, S., Coppel, R., Alexander, I. (2009). Antigen-specific humoral tolerance or immune augmentation induced by intramuscular delivery of adeno-associated viruses encoding CTLA4-Ig-antigen fusion molecules. Gene Therapy (Basingstoke), 16(2), 200-210. [More Information]
  • Laurence, J., McCaughan, G., Logan, G., Alexander, I., Bishop, A., Sharland, A. (2009). Gene therapy in transplantation. Transplantation Reviews, 23(3), 159-170. [More Information]
  • Cunningham, S., Zoccai, G., Spinoulas, A., Carpenter, K., Alexander, I. (2009). In vivo assessment of mutations in OTC for dominant-negative effects following rAAV2/8-mediated gene delivery to the mouse liver. Gene Therapy, 16(6), 820-823. [More Information]
  • Liu, R., Ginn, S., Lek, M., North, K., Alexander, I., Little, D., Schindeler, A. (2009). Myoblast sensitivity and fibroblast insensitivity to osteogenic conversion by BMP-2 correlates with the expression of Bmpr-1 a. BMC Musculoskeletal Disorders, 10, 51-1-51-12. [More Information]
  • Laurence, J., Wang, C., Zheng, M., Cunningham, S., Earl, J., Tay, S., Allen, R., McCaughan, G., Alexander, I., Bishop, A., Sharland, A. (2009). Overexpression of indoleamine dioxygenase in rat liver allografts using a high-efficiency adeno-associated virus vector does not prevent acute rejection. Liver Transplantation, 15(2), 233-241. [More Information]
  • Dane, A., Cunningham, S., Graf, N., Alexander, I. (2009). Sexually Dimorphic Patterns of Episomal rAAV Genome Persistence in the Adult Mouse Liver and Correlation With Hepatocellular Proliferation. Molecular Therapy, 17(9), 1548-1554. [More Information]

2008

  • Curtin, J., Dane, A., Swanson, A., Alexander, I., Ginn, S. (2008). Bidirectional promoter interference between two widely used internal heterologous promoters in a late-generation lentiviral construct. Gene Therapy (Basingstoke), 15(5), 384-390. [More Information]
  • Baoutina, A., Alexander, I., Rasko, J., Emslie, K. (2008). Developing strategies for detection of gene doping. Journal of Gene Medicine, 10, 3-20. [More Information]
  • Cunningham, S., Dane, A., Spinoulas, A., Alexander, I. (2008). Gene delivery to the juvenile mouse liver using AAV2/8 vectors. Molecular Therapy, 16(6), 1081-1088. [More Information]
  • Alexander, I., Cunningham, S., Logan, G., Christodoulou, J. (2008). Potential of AAV vectors in the treatment of metabolic disease. Gene Therapy, 15(11), 831-839. [More Information]
  • Lee, V., Wang, Y., Wang, Y., Zheng, D., Polhill, T., Cao, Q., Wu, H., Alexander, I., Alexander, S., Harris, D. (2008). Regulatory immune cells in kidney disease. American Journal of Physiology: Renal Physiology (Print), 295(2), F335-F342. [More Information]

2007

  • Cooper, S., Kizana, E., Yates, J., Lo, H., Yang, N., Wu, Z., Alexander, I., North, K. (2007). Dystrophinopathy carrier determination and detection of protein deficiencies in muscular dystrophy using lentiviral MyoD-forced myogenesis. Neuromuscular Disorders, 17(4), 276-284. [More Information]
  • Kizana, E., Chang, C., Cingolani, E., Sekar, R., Ramirez-Correa, G., Abraham, M., Ginn, S., Tung, L., Alexander, I., Marban, E. (2007). Gene transfer of connexin43 mutants attenuates coupling in cardiomyocytes: novel basis for modulation of cardiac conduction by gene therapy. Circulation Research. [More Information]
  • Yu, Z., McKay, K., Van Asperen, P., Zheng, M., Fleming, J., Ginn, S., Kizana, E., Latham, M., Feneley, M., Kirkland, P., Lumbers, E., Alexander, I., et al (2007). Lentivirus vector-mediated gene transfer to the developing bronchiolar airway epithelium in the fetal lamb. Journal of Gene Medicine, 9(6), 429-439. [More Information]
  • Smyth, C., Ginn, S., Deakin, C., Logan, G., Alexander, I. (2007). Limiting {gamma}c expression differentially affects signaling via the interleukin (IL)-7 and IL-15 receptors. Blood, 110(1), 91-98. [More Information]
  • Ganesan, P., Alexander, S., Watson, D., Logan, G., Zhang, G., Alexander, I. (2007). Robust anti-tumor immunity and memory in Rag-1-deficient mice following adoptive transfer of cytokine-primed splenocytes and tumor CD80 expression. Cancer Immunology, Immunotherapy: other biological response modifications, 56(12), 1955-1965. [More Information]
  • Deichmann, A., Hacein-Bey-Abina, S., Schmidt, M., Garrigue, A., Brugman, M., Hu, J., Glimm, H., Gyapay, G., Prum, B., et, A., Alexander, I. (2007). Vector integration is nonrandom and clustered and influences the fate of lymphopoiesis in SCID-X1 gene therapy. Journal of Clinical Investigation, 117(8), 2225-2232. [More Information]

2006

  • Logan, G., Wang, L., Zheng, M., Cunningham, S., Coppel, R., Alexander, I. (2006). AAV vectors encoding malarial antigens stimulate antigen-specific immunity but do not protect from parasite infection. Vaccine, 25(6), 1014-1022. [More Information]
  • Kramer, B., Lemckert, F., Alexander, I., Gunning, P., McCowage, G. (2006). Characterisation of a P140K mutant O(6)-methylguanine-DNA-methyltransferase (MGMT)-expressing transgenic mouse line with drug-selectable bone marrow. Journal of Gene Medicine, 8(9), 1071-1085. [More Information]
  • Kizana, E., Ginn, S., Smyth, C., Boyd, A., Thomas, S., Allen, D., Ross, D., Alexander, I. (2006). Fibroblasts modulate cardiomyocyte excitability: implications for cardiac gene therapy. Gene Therapy (Basingstoke), 13(22), 1611-1615. [More Information]
  • Wang, Y., Zhang, G., Wang, Y., Hu, M., Wu, H., Watson, D., Hori, S., Alexander, I., Harris, D., Alexander, S. (2006). Foxp3-transduced polyclonal regulatory T cells protect against chronic renal injury from adriamycin. Journal of the American Society of Nephrology, 17(3), 697-706. [More Information]
  • Trent, R., Alexander, I. (2006). Gene therapy in sport. British Journal of Sports Medicine, 40(1), 4-5. [More Information]
  • Hu, M., Kramer, B., Zhang, G., McCowage, G., Alexander, I., Gunning, P., Alexander, S. (2006). Long term skin allograft survival in mice with MGMT chimeric bone marrow engraftment. Immunology and Cell Biology, 84 (Suppl.)(3), A29-A29.
  • Hu, M., Kramer, B., Zhang, G., McCowage, G., Alexander, I., Gunning, P., Alexander, S. (2006). Long term skin allograft survival in mice with MGMT chimeric bone marrow engraftment. 24th Annual Scientific Meeting of the Transplantation Society of Australia and New Zealand (2006), Australia: Nature Publishing Group.

2005

  • Alexander, I., Logan, G., Logan, G., Boadle, R., Rowe, P., Smythe, J. (2005). Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy. International Immunology, 17(2), 123-132. [More Information]
  • Smyth, C., Logan, G., Boadle, R., Rowe, P., Smythe, J., Alexander, I. (2005). Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy. International Immunology, 17(2), 123-132.
  • Kizana, E., Ginn, S., Allen, D., Ross, D., Alexander, I. (2005). Fibroblasts can be genetically modified to produce excitable cells capable of electrical coupling. Circulation, 111(4), 394-398. [More Information]
  • Kizana, E., Ginn, S., Smyth, C., Thomas, S., Allen, D., Ross, D., Alexander, I. (2005). Fibroblasts Modulate Cardiomyocyte Excitability: Insights from Fibroblasts with Genetically Altered Connexin43 Expression. Journal of Gene Medicine, 7(point P8 on page 1127), 1127-1127.
  • Fleming, J., Spinoulas, A., Zheng, M., Cunningham, S., Ginn, S., McQuilty, R., Rowe, P., Alexander, I. (2005). Partial correction of sensitivity to oxidant stress in Friedreich ataxia patient fibroblasts by frataxin-encoding adeno-associated virus and lentivirus vectors. Human Gene Therapy, 16(8), 947-956. [More Information]
  • Mitchell, J., Wilcken, B., Alexander, I., Ellaway, C., O'Grady, H., Wiley, V., Earl, J., Christodoulou, J. (2005). Tetrahydrobiopterin-responsive phenylketonuria: The New South Wales experience. Molecular Genetics and Metabolism, 86(Suppl 1), S81-S85. [More Information]
  • Ginn, S., Curtin, J., Kramer, B., Smyth, C., Wong, M., Kakakios, A., McCowage, G., Watson, D., Alexander, S., Latham, M., Rowe, P., Alexander, I., et al (2005). Treatment of an infant with X-linked severe combined immunodeficiency (SCID-X1) by gene therapy in Australia. Medical Journal of Australia, 182(9), 458-463. [More Information]

2004

  • Ginn, S., Smyth, C., Wong, M., Bennetts, B., Rowe, P., Alexander, I. (2004). A Novel Splice-Site Mutation In The Common Gamma Chain (Gammac) Gene Il2Rg Results In X-Linked Severe Combined Immunodeficiency With An Atypical Nk+ Phenotype. Human Mutation, 23(5), 522-523.
  • Allen, D., Hardeman, E., North, K., Alexander, I., Cooper, S., Maxwell, A., Kizana, E., Ghoddusi, M. (2004). C2C12 Co-Culture On A Fibroblast Substratum Enables Sustained Survival Of Contractile, Highly Differentiated Myotubes With Peripheral Nuclei And Adult Fast Myosin Expression. Cell Motility and the Cytoskeleton, 58(3), 200-211. [More Information]
  • Logan, G., Spinoulas, A., Alexander, S., Smythe, J., Alexander, I. (2004). CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection. Immunology and Cell Biology, 82(2), 132-135.
  • Trent, R., Alexander, I. (2004). Gene Therapy: Applications And Progress Towards The Clinic. Internal Medicine Journal, 34(11), 621-625.

2003

  • Alexander, I., Kizana, E. (2003). Cardiac gene therapy: therapeutic potential and current progress. Current Gene Therapy: the international journal for in-depth reviews on gene therapy, 3(5), 418-451.
  • Fleming, J., Ginn, S., Alexander, I. (2003). Dorsal root ganglia sensory neurons. In Federico (Eds.), Lentivirus Gene Engineering Protocols, (pp. 155-167). New Jersey: Humana Press.
  • Ginn, S., Fleming, J., Curtin, J., Alexander, I. (2003). Gene therapy: a roller-coaster ride. Today's Life Science (Australia), 15(3), 26-29.
  • Hacein-Bey-Abina, S., Von Kalle, C., Schmidt, M., McCormack, M., Wulffraat, N., Leboulch, P., Lim, A., Osborne, C., Pawliuk, R., Morillon, E., Alexander, I., et al (2003). LMO2-associated clonal T Cell proliferation in two patients after gene therapy for SCID-X1. Science, 302(5644), 415-419.
  • Ginn, S., Fleming, J., Rowe, P., Alexander, I. (2003). Promoter interference mediated by the U3 Region in early-generation HIV-1-Derived Lentivirus Vectors can influence detection of transgene expression in a cell-type and species-specific manner. Human Gene Therapy, 14(12), 1127-1137.

2002

  • Alexander, I. (2002). Australasian Gene Therapy Society kicks off. Journal of Gene Medicine, , 4-4.
  • Logan, G., Smyth, C., Earl, J., Rowe, P., Zaikina, I., Smythe, J., Alexander, I. (2002). HeLa cells cocultured with periopheral blood lymphocytes acquire an immuno-inhibitory phenotype through up-regulation of indoleamine 2,3-dioxygenase activity. Immunology, 105(4), 478-487.

To update your profile click here. For support on your academic profile contact .