Dr James Melrose

Honorary Senior Research Fellow
Surgery, Northern Clinical School

Telephone +61 2 9926 4806
Fax +61 2 9926 5266

Map

International links

Australia

(School of Biomedical Engineering, University of New South Wales, Kensington, NSW 2052) Collaboration with Prof John Whitelock on the modular HS-proteoglycan, perlecan in health and disease in tensional and weight bearing connective tissues

Canada

(McGill University) Collaboration with Prof Peter Roughley, McGill University, Canada on various aspects of connective tissue proteoglycans particularly intervertebral disc in health and disease

United Kingdom

(BioImaging Unit, Cardiff School of Biosciences, University of Cardiff) Dr Anthony Hayes, Collaboration on the imaging of tensional and weight bearing connective tissues including, intervertebral disc, articular cartilage, meniscus, tendon and the role of CS sulphation motifs in early human foetal development

United Kingdom

(Cardiff School of Biosciences, University of Cardiff) Prof Bruce caterson, Dr Clare E Hughes. The role of CS sulphation motifs in the development of human foetal tensional and weight bearing connective tissues including, knee, hip, elbow, spine

United Kingdom

(Robert Jones & Agnes Hunt Orthopaedic Hospital, Oswestry, UK and The Institute of Science & Technology in Medicine, Keele University, UK.) Collaboration with Professor Sally Roberts, Director of Spinal Research, Robert Jones & Agnes Hunt Orthopaedic Hospital, Oswestry, UK on various aspects of intervertebral disc structure, function and physiology in health and disease.

Selected grants

2011

  • Mesenchymal Progenitor cells and intervertebral disc repair; Melrose J, Appleyard R, Little C; National Health and Medical Research Council (NHMRC)/Project Grants.

2009

  • Stem Cell treatment for tendon degeneration; Smith M, Little C, Smith M, Sonnabend D, Appleyard R, Melrose J, Hunyor S; National Health and Medical Research Council (NHMRC)/Project Grants.

2008

  • An in-vitro model of meniscal degeneration initiated by inflammatory cytokines; Melrose J, Little C; Arthritis Foundation of Australia/Research Support.
  • The role of perlacan in tensional connective tissues; Melrose J, Whitelock J, Hayes A, Whitelock J, Appleyard R, Little C; National Health and Medical Research Council (NHMRC)/Project Grants.

2006

  • Proteoglycan metabolism in tendon degeneration; Little C, Sonnabend D, Appleyard R, Melrose J, Fosang A; National Health and Medical Research Council (NHMRC)/Project Grants.

2005

  • Pathobiology of the small leucine rich repeat proteoglycans in cartilage, intervertebral disc and tendon degeneration; Melrose J, Little C, Sonnabend D; National Health and Medical Research Council (NHMRC)/Project Grants.

2004

  • The role of cathepsin K in musculoskeletal disorders: potential modulation by pentosan polysulphate.; Little C, Smith M, Melrose J; Sylvan Pharmaceuticals Pty Ltd/Research Grant.
  • Laboratory studies on articular cartilage and synovium to evaluate the effects of intra-articular treatments of hyaluronan (Hyalgan-E, Hyalgan-F and /or HYADD4) in an ovine model of osteoarthritis.; Little C, Smith M, Melrose J; FIDIA Pharmaceuticals/Research Grant.

2002

  • Investigations on the regulation of intervertebral disc cell matrix metalloproteinases; Melrose J, Ghosh P; National Health and Medical Research Council (NHMRC)/Project Grants.

2000

  • Mechanisms of disc degeneration in the lumbar spine; Ghosh P, Melrose J, Taylor T; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Proteoglycan catabolism in an ovine annular lesion model of disc degeneration; Ghosh P, Melrose J, Taylor T, Bellenger C; National Health and Medical Research Council (NHMRC)/Project Grants.

Selected publications

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Book Chapters

  • Whitelock, J., Melrose, J. (2006). Adhesion of Cells to Biomaterials. In Metin Akay (Eds.), Wiley Encyclopedia of Biomedical Engineering. United States: Wiley-Blackwell Publishing.
  • Melrose, J., Whitelock, J. (2006). Extracellular Matrix. In Metin Akay (Eds.), Wiley Encyclopedia of Biomedical Engineering. United States: Wiley-Blackwell Publishing.
  • Melrose, J. (2001). Cartilage and smooth muscle cell proteoglycans detected by affinity blotting using biotinylated hyaluronan. In Iozzo RV (Eds.), Proteoglycan Protocols, (pp. 53-66). United States: Humana Press.

Journals

  • Hodges, P., James, G., Blomster, L., Hall, L., Schmid, A., Shu, C., Little, C., Melrose, J. (2014). Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion? Spine, 39(13), 1010-1017. [More Information]
  • Lord, M., Chuang, C., Melrose, J., Davies, M., Iozzo, R., Whitelock, J. (2014). The role of vascular-derived perlecan in modulating cell adhesion, proliferation and growth factor signaling. Matrix Biology, 35, 112-122. [More Information]
  • Melrose, J., Smith, M., Smith, S., Ravi, V., Young, A., Dart, A., Sonnabend, D., Little, C. (2013). Altered stress induced by partial transection of the infraspinatus tendon leads to perlecan (HSPG2) accumulation in an ovine model of tendinopathy. Tissue and Cell, 45(1), 77-82. [More Information]
  • Hayes, A., Smith, S., Melrose, J. (2013). Comparative immunolocalisation of fibrillin-1 and perlecan in the human foetal, and HS-deficient hspg2 exon 3 null mutant mouse intervertebral disc. Histochemistry and Cell Biology, 139(1), 1-11. [More Information]
  • Shu, C., Smith, S., Little, C., Melrose, J. (2013). Comparative immunolocalisation of perlecan, heparan sulphate, fibroblast growth factor-18, and fibroblast growth factor receptor-3 and their prospective roles in chondrogenic and osteogenic development of the human foetal spine. European Spine Journal, 22(8), 1774-1784. [More Information]
  • Shu, C., Hughes, C., Smith, S., Smith, M., Hayes, A., Caterson, B., Little, C., Melrose, J. (2013). The ovine newborn and human foetal intervertebral disc contain perlecan and aggrecan variably substituted with native 7D4 CS sulphation motif: spatiotemporal immunolocalisation and co-distribution with Notch-1 in the human foetal disc. Glycoconjugate Journal, 30(7), 717-725. [More Information]
  • Brown, S., Melrose, J., Caterson, B., Roughley, P., Eisenstein, S., Roberts, S. (2012). A comparative evaluation of the small leucine-rich proteoglycans of pathological human intervertebral discs. European Spine Journal, 21(Suppl 2), S154-S159. [More Information]
  • Melrose, J., Isaacs, M., Smith, S., Hughes, C., Little, C., Caterson, B., Hayes, A. (2012). Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development. Histochemistry and Cell Biology, 138(3), 461-475. [More Information]
  • Melrose, J., Shu, C., Young, C., Ho, R., Smith, M., Young, A., Smith, S., Gooden, B., Dart, A., Podadera, J., Appleyard, R., Little, C. (2012). Mechanical destabilization induced by controlled annular incision of the intervertebral disc dysregulates metalloproteinase expression and induces disc degeneration. Spine, 37(1), 18-25. [More Information]
  • Chuang, C., Shahin, K., Lord, M., Melrose, J., Doran, P., Whitelock, J. (2012). The cartilage matrix molecule components produced by human foetal cartilage rudiment cells within scaffolds and the role of exogenous growth factors. Biomaterials, 33(16), 4078-4088. [More Information]
  • Fuller, E., Smith, M., Little, C., Melrose, J. (2012). Zonal differences in meniscus matrix turnover and cytokine response. Osteoarthritis and Cartilage, 20(1), 49-59. [More Information]
  • Hayes, A., Lord, M., Smith, S., Smith, M., Whitelock, J., Weiss, A., Melrose, J. (2011). Colocalization in vivo and association in vitro of perlecan and elastin. Histochemistry and Cell Biology, 136, 437-454. [More Information]
  • Hayes, A., Smith, S., Gibson, M., Melrose, J. (2011). Comparative immunolocalisation of the elastin fibre associated proteins fibrillin-1, LTBP2 and MAGP-1 with components of the collagenous and proteoglycan matrix of the foetal human Intervertebral Disc. Spine, 36(21), E1365-1372. [More Information]
  • Whitelock, J., Melrose, J. (2011). Heparan sulfate proteoglycans in healthy and diseased systems. Wiley Interdisciplinary Reviews. Systems Biology and Medicine, 3(6), 739-751. [More Information]
  • Smith, S., Melrose, J. (2011). Podoplanin is expressed by a sub-population of human foetal rib and knee joint rudiment chondrocytes. Tissue and Cell, 43(1), 39-44. [More Information]
  • Stanton, H., Melrose, J., Little, C., Fosang, A. (2011). Proteoglycan degradation by the ADAMTS family of proteinases. Biochimica et Biophysica Acta-Molecular Basis of Disease, 1812 (12), 1616-1629. [More Information]
  • Schollum, M., Appleyard, R., Little, C., Melrose, J. (2010). A Detailed Microscopic Examination of Alterations in Normal Anular Structure Induced by Mechanical Destabilization in an Ovine Model of Disc Degeneration. Spine, 35(22), 1965-1973. [More Information]
  • Smith, S., Shu, C., Melrose, J. (2010). Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker. Histochemistry and Cell Biology, 134(3), 251-263. [More Information]
  • Chuang, C., Lord, M., Melrose, J., Rees, M., Knox, S., Freeman, C., Iozzo, R., Whitelock, J. (2010). Heparan Sulfate-Dependent Signaling of Fibroblast Growth Factor 18 by Chondrocyte-Derived Perlecan. Biochemistry, 49(26), 5524-5532. [More Information]
  • Melrose, J., Little, C. (2010). Immunolocalization of lymphatic vessels in human fetal knee joint tissues. Connective Tissue Research, 51(4), 289-305. [More Information]
  • Melrose, J., Burkhardt, D., Taylor, T., Dillon, C., Read, R., Cake, M., Little, C. (2009). Calcification in the ovine intervertebral disc: a model of hydroxyapatite deposition disease. European Spine Journal, 18(4), 479-489. [More Information]
  • Smith, S., Whitelock, J., Iozzo, R., Little, C., Melrose, J. (2009). Topographical variation in the distributions of versican, aggrecan and perlecan in the foetal human spine reflects their diverse functional roles in spinal development. Histochemistry and Cell Biology, 132(5), 491-503. [More Information]
  • Whitelock, J., Melrose, J., Iozzo, R. (2008). Diverse Cell Signaling Events Modulated by Perlecan. Biochemistry, 47(43), 11174-11183. [More Information]
  • Melrose, J., Fuller, E., Roughley, P., Smith, M., Kerr, B., Hughes, C., Caterson, B., Little, C. (2008). Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degenerate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Arthritis Research and Therapy, 10(doi:10.1186/ar2453), 1-10. [More Information]
  • Cake, M., Melrose, J. (2008). Intervertebral disc is an alternate tissue source of circulating C-telopeptide of type II collagen after menopause or ovariectomy: comment on the article by Sondergaard et al. Arthritis and Rheumatism, 58(5), 1560. [More Information]
  • Smith, M., Sakurai, G., Smith, S., Young, A., Melrose, J., Stewart, C., Appleyard, R., Peterson, J., Gilles, R., Dart, A., Sonnabend, D., Little, C. (2008). Modulation of Aggrecan and ADAMTS Expression in Ovine Tendinopathy Induced by Altered Strain. Seminars in Arthritis and Rheumatism, 58(4), 1055-1066. [More Information]
  • Melrose, J., Hayes, A., Whitelock, J., Little, C. (2008). Perlecan, the jack of all trades" proteoglycan of cartilaginous weight-bearing connective tissues.". BioEssays : news and reviews in molecular cellular and developmental biology, 30(5), 457-469. [More Information]
  • Melrose, J., Smith, S., Little, C., Moore, R., Vernon-Roberts, B., Fraser, R. (2008). Recent advances in annular pathobiology provide insights into rim-lesion mediated intervertebral disc degeneration and potential new approaches to annular repair strategies. European Spine Journal, 17(9), 1131-1148. [More Information]
  • Melrose, J., Smith, S., Smith, M., Little, C. (2008). The use of Histochoice (tmR) for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus. Biotechnic and Histochemistry, 83(1), 47-53. [More Information]
  • Melrose, J., Chuang, C., Whitelock, J. (2008). Tissue engineering of cartilages using biomatrices. Journal of Chemical Technology and Biotechnology, 83, 444-463. [More Information]
  • Melrose, J., Smith, S., Appleyard, R., Little, C. (2007). Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc. European Spine Journal, Published online: 31 October 2007, 1-11. [More Information]
  • Alini, M., Eisenstein, S., Ito, K., Little, C., Kettler, A., Masuda, K., Melrose, J., Ralphs, J., Stokes, I., Wilke, H. (2007). Are animal models useful for studying human disc disorders/degeneration? European Spine Journal, Published online: 14 July 2007, 1-18. [More Information]
  • Melrose, J., Smith, S., Fuller, E., Young, A., Roughley, P., Dart, A., Little, C. (2007). Biglycan and fibromodulin fragmentation correlates with temporal and spatial annular remodelling in experimentally injured ovine intervertebral discs. European Spine Journal, 16(12), 2193-2205. [More Information]
  • Young, A., Appleyard, R., Smith, M., Melrose, J., Little, C. (2007). Dynamic Biomechanics Correlate With Histopathology in Human Tibial Cartilage: A Preliminary Study. Clinical Orthopaedics and Related Research, 462, 212-220. [More Information]
  • Young, A., McLennan, S., Smith, M., Smith, S., Cake, M., Read, R., Melrose, J., Sonnabend, D., Flannery, C., Little, C. (2006). Proteoglycan 4 downregulation in a sheep meniscectomy model of early osteoarthritis. Arthritis Research and Therapy, 8(2), 1-6. [More Information]
  • Melrose, J., Roughley, P., Knox, S., Smith, S., Lord, M., Whitelock, J. (2006). The structure, location and function of perlecan, a prominent pericellular proteoglycan of foetal, postnatal and mature hyaline cartilages. Journal of Biological Chemistry, 281(48), 36905-36914. [More Information]
  • Melrose, J., Smith, S., Cake, M., Read, R., Whitelock, J. (2005). Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study. Histochemistry and Cell Biology, 124(3-4), 225-235. [More Information]
  • Young, A., Smith, M., Smith, S., Burkhardt, D., Cake, M., Melrose, J., Read, R., Ghosh, P., Sonnabend, D., Little, C. (2005). Metabolism of Small Leucine-Rich Proteoglycans in an Ovine Model of Osteoarthritis. 51st Annual Meeting of the Orthopaedic Research Society, 30, 1057-1057.
  • Melrose, J., Smith, S., Cake, M., Read, R., Whitelock, J. (2005). Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint. Histochemistry and Cell Biology, 123(6), 561-571. [More Information]
  • Knox, S., Fosang, A., Last, K., Melrose, J., Whitelock, J. (2005). Perlecan from human epithelial cells is a hybrid heparan/chondroitin/keratan sulfate proteoglycan. FEBS Letters, 579(22), 5019-5023. [More Information]
  • Young, A., Smith, M., Smith, S., Cake, M., Ghosh, P., Read, R., Melrose, J., Sonnabend, D., Roughley, P., Little, C. (2005). Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis. Arthritis Research and Therapy, 7(4), R852-61. [More Information]
  • Melrose, J., Smith, S. (2004). Histochemical Visualization Of The Cartilage Hyaladherins Using A Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe. Methods in molecular medicine, 101(1), 65-78.
  • Melrose, J., Smith, S., Ghosh, P. (2004). Histological And Immunohistological Studies On Cartilage. Methods in molecular medicine, 101(1), 39-63.
  • Melrose, J., Smith, S., Whitelock, J. (2004). Perlecan Immunolocalizes To Perichondrial Vessels And Canals In Human Fetal Cartilaginous Primordia In Early Vascular And Matrix Remodeling Events Associated With Diarthrodial Joint Development. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 52(11), 1405-1413.
  • Melrose, J., Smith, S., Ghosh, P. (2003). Assessment of the cellular heterogeneity of the ovine intervertebral disc: comparison with synovial fibroblasts and articular chondrocytes. European Spine Journal, 12(1), 57-65.
  • Shen, B., Melrose, J., Ghosh, P., Taylor, T. (2003). Induction of matrix metalloproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in three-dimensional agarose gel culture by interleukin-1beta: a potential pathway of disc degeneration. European Spine Journal, 12(1), 66-75.
  • Melrose, J., Smith, S., Ghosh, P., Whitelock, J. (2003). Perlecan, the multidomain heparan sulfate proteoglycan of basement membranes, is also a prominent component of the cartilaginous primordia in the developing human fetal spine. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 51(10), 1331-1341.
  • Melrose, J., Roberts, S., Smith, S., Menage, J., Ghosh, P. (2002). Increased Nerve and Blood Vessel Ingrowth Associated With Proteoglycan Depletion in an Ovine Anular Lesion Model of Experimental Disc Degeneration. Spine, 27(12), 1278-1285.
  • Knox, S., Merry, C., Stringer, S., Melrose, J., Whitelock, J. (2002). Not All Perlecans Are Created Equal. Journal of Biological Chemistry, 277(17), 14657-14665.
  • Melrose, J., Smith, S., Knox, S., Whitelock, J. (2002). Perlecan, the multidomain HS-proteoglycan of basement membranes, is a prominent pericellular component of ovine hypertrophic vertebral growth plate and cartilaginous endplate chondrocytes. Histochemistry and Cell Biology, 118(4), 269-280.
  • Melrose, J., Smith, S., Little, C., Kitson, J., Hwa, S., Ghosh, P. (2002). Spatial and temporal localization of transforming growth factor-b, fibroblast growth factor-2, and osteonectin, and identification of cells expressing a-smooth muscle actin in the injured annulus fibrosus. Implications for extracellular matrix repair. Spine, 27(16), 1756-1764.
  • Melrose, J., Smith, S., Ghosh, P. (2002). Synthesis of a Kunitz-Type Serine Proteinase Inhibitory Protein That Shares Homology with Bovine Pancreatic Trypsin Inhibitor by Ovine Intervertebral Disc Cells in Serum-Free alginate Bead Culture. Journal of Spinal Disorders and Techniques, 15(2), 164-171.
  • Melrose, J., Tammi, M., Smith, S. (2002). Visualisation of hyaluronan and hyaluronan-binding proteins within ovine vertebral cartilages using biotinylated aggrecan G1-link complex and biotinylated hyaluronan oligosaccharides. Histochemistry and Cell Biology, 117(4), 327-333. [More Information]
  • Melrose, J., Ghosh, P., Taylor, T. (2001). A comparative analysis of the differential spatial and temporal distributions of the large (aggreca. Journal of Anatomy, 198, 3-15.
  • Melrose, J., Ghosh, P., Taylor, T. (2001). A comparative analysis of the differential spatial and temporal distributions of the large (aggrecan, versican) and small (decorin, biglycan, fibromodulin) proteoglycans of the intervertebral disc. Journal of Anatomy, 198(1), 3-15.
  • Melrose, J., Ghosh, P., Shen, B. (2001). Affinity and western blotting reveal homologies between ovine intervertebral disc serine proteinase. Proteomics, 1, 1529-1533.
  • Melrose, J., Ghosh, P., Taylor, T., Smith, S. (2001). Differential expression of proteoglycan epitopes and growth characteristics of intervertebral disc. Cells Tissues Organs, 168(3), 137-146.
  • Melrose, J., Knox,, S., Whitelock, J. (2001). Electrophoretic, biosensor, and bioactivity analyses of perlecans of different molecular size. Proteomics, 1, 1534-1541.
  • Taylor, T., Melrose, J., Burkhardt, D., Ghosh, P., Kettler, A., Wilke, H., Claes, L. (2000). Spinal biomechanics and aging are major determinants of the proteoglycan metabolism of intervertebral disc cells. Spine, 25(23), 3014-3020.

Conferences

  • Melrose, J., Gooden, B., Dart, A., Appleyard, R., Little, C. (2009). Dysregulation of metalloproteinase expression in a mechanical destabilization model of intervertebral disc degeneration in sheep. ACSR Spinal Research Symposium VII.
  • Melrose, J., Smith, S., Fuller, E., Young, A., Roughley, P., Dart, A., Little, C. (2008). Spatial and temporal catabolism of the small leucine repeat proteoglycans (SLRPs) in an ovine annular lesion model of experimental intervertebral disc degeneration has structure-function implications. World Forum for Spine Research 2008, Switzerland: AO Foundation.
  • Smith, M., Sakurai, A., Smith, S., Young, A., Melrose, J., Dart, A., Sonnabend, D., Little, C. (2006). Altered aggrecan and ADAMTS expression in overload-induced tendon pathology. 49th Annual Meeting of the Orthopaedic Research Society, United states: Orthopaedic Research Society.
  • Young, A., McLennan, S., Smith, S., Smith, M., Melrose, J., Cake, M., Flannery, C., Sonnabend, D., Little, C. (2006). Changes in expression and immunolcalization of proteoglycan 4 / lubricin in cartilage in an ovine model of early osteoarthritis. 49th Annual Meeting of the Orthopaedic Research Society, United states: Orthopaedic Research Society.
  • Melrose, J., Smith, S., Little, C., Kitson,, J., Hwa, S., Ghosh, P. (2001). Repair of annular disc lesions: Spatial and temporal localisation of TGF-beta, SPARC (Osteonectin, BM-40) and FGF-2 are early responses in experimentally injured ovine discs. 47th Annual Meeting of the Orthopaedic Research Society, USA: Orthopaedic Research Society.

2014

  • Hodges, P., James, G., Blomster, L., Hall, L., Schmid, A., Shu, C., Little, C., Melrose, J. (2014). Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion? Spine, 39(13), 1010-1017. [More Information]
  • Lord, M., Chuang, C., Melrose, J., Davies, M., Iozzo, R., Whitelock, J. (2014). The role of vascular-derived perlecan in modulating cell adhesion, proliferation and growth factor signaling. Matrix Biology, 35, 112-122. [More Information]

2013

  • Melrose, J., Smith, M., Smith, S., Ravi, V., Young, A., Dart, A., Sonnabend, D., Little, C. (2013). Altered stress induced by partial transection of the infraspinatus tendon leads to perlecan (HSPG2) accumulation in an ovine model of tendinopathy. Tissue and Cell, 45(1), 77-82. [More Information]
  • Hayes, A., Smith, S., Melrose, J. (2013). Comparative immunolocalisation of fibrillin-1 and perlecan in the human foetal, and HS-deficient hspg2 exon 3 null mutant mouse intervertebral disc. Histochemistry and Cell Biology, 139(1), 1-11. [More Information]
  • Shu, C., Smith, S., Little, C., Melrose, J. (2013). Comparative immunolocalisation of perlecan, heparan sulphate, fibroblast growth factor-18, and fibroblast growth factor receptor-3 and their prospective roles in chondrogenic and osteogenic development of the human foetal spine. European Spine Journal, 22(8), 1774-1784. [More Information]
  • Shu, C., Hughes, C., Smith, S., Smith, M., Hayes, A., Caterson, B., Little, C., Melrose, J. (2013). The ovine newborn and human foetal intervertebral disc contain perlecan and aggrecan variably substituted with native 7D4 CS sulphation motif: spatiotemporal immunolocalisation and co-distribution with Notch-1 in the human foetal disc. Glycoconjugate Journal, 30(7), 717-725. [More Information]

2012

  • Brown, S., Melrose, J., Caterson, B., Roughley, P., Eisenstein, S., Roberts, S. (2012). A comparative evaluation of the small leucine-rich proteoglycans of pathological human intervertebral discs. European Spine Journal, 21(Suppl 2), S154-S159. [More Information]
  • Melrose, J., Isaacs, M., Smith, S., Hughes, C., Little, C., Caterson, B., Hayes, A. (2012). Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development. Histochemistry and Cell Biology, 138(3), 461-475. [More Information]
  • Melrose, J., Shu, C., Young, C., Ho, R., Smith, M., Young, A., Smith, S., Gooden, B., Dart, A., Podadera, J., Appleyard, R., Little, C. (2012). Mechanical destabilization induced by controlled annular incision of the intervertebral disc dysregulates metalloproteinase expression and induces disc degeneration. Spine, 37(1), 18-25. [More Information]
  • Chuang, C., Shahin, K., Lord, M., Melrose, J., Doran, P., Whitelock, J. (2012). The cartilage matrix molecule components produced by human foetal cartilage rudiment cells within scaffolds and the role of exogenous growth factors. Biomaterials, 33(16), 4078-4088. [More Information]
  • Fuller, E., Smith, M., Little, C., Melrose, J. (2012). Zonal differences in meniscus matrix turnover and cytokine response. Osteoarthritis and Cartilage, 20(1), 49-59. [More Information]

2011

  • Hayes, A., Lord, M., Smith, S., Smith, M., Whitelock, J., Weiss, A., Melrose, J. (2011). Colocalization in vivo and association in vitro of perlecan and elastin. Histochemistry and Cell Biology, 136, 437-454. [More Information]
  • Hayes, A., Smith, S., Gibson, M., Melrose, J. (2011). Comparative immunolocalisation of the elastin fibre associated proteins fibrillin-1, LTBP2 and MAGP-1 with components of the collagenous and proteoglycan matrix of the foetal human Intervertebral Disc. Spine, 36(21), E1365-1372. [More Information]
  • Whitelock, J., Melrose, J. (2011). Heparan sulfate proteoglycans in healthy and diseased systems. Wiley Interdisciplinary Reviews. Systems Biology and Medicine, 3(6), 739-751. [More Information]
  • Smith, S., Melrose, J. (2011). Podoplanin is expressed by a sub-population of human foetal rib and knee joint rudiment chondrocytes. Tissue and Cell, 43(1), 39-44. [More Information]
  • Stanton, H., Melrose, J., Little, C., Fosang, A. (2011). Proteoglycan degradation by the ADAMTS family of proteinases. Biochimica et Biophysica Acta-Molecular Basis of Disease, 1812 (12), 1616-1629. [More Information]

2010

  • Schollum, M., Appleyard, R., Little, C., Melrose, J. (2010). A Detailed Microscopic Examination of Alterations in Normal Anular Structure Induced by Mechanical Destabilization in an Ovine Model of Disc Degeneration. Spine, 35(22), 1965-1973. [More Information]
  • Smith, S., Shu, C., Melrose, J. (2010). Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker. Histochemistry and Cell Biology, 134(3), 251-263. [More Information]
  • Chuang, C., Lord, M., Melrose, J., Rees, M., Knox, S., Freeman, C., Iozzo, R., Whitelock, J. (2010). Heparan Sulfate-Dependent Signaling of Fibroblast Growth Factor 18 by Chondrocyte-Derived Perlecan. Biochemistry, 49(26), 5524-5532. [More Information]
  • Melrose, J., Little, C. (2010). Immunolocalization of lymphatic vessels in human fetal knee joint tissues. Connective Tissue Research, 51(4), 289-305. [More Information]

2009

  • Melrose, J., Burkhardt, D., Taylor, T., Dillon, C., Read, R., Cake, M., Little, C. (2009). Calcification in the ovine intervertebral disc: a model of hydroxyapatite deposition disease. European Spine Journal, 18(4), 479-489. [More Information]
  • Melrose, J., Gooden, B., Dart, A., Appleyard, R., Little, C. (2009). Dysregulation of metalloproteinase expression in a mechanical destabilization model of intervertebral disc degeneration in sheep. ACSR Spinal Research Symposium VII.
  • Smith, S., Whitelock, J., Iozzo, R., Little, C., Melrose, J. (2009). Topographical variation in the distributions of versican, aggrecan and perlecan in the foetal human spine reflects their diverse functional roles in spinal development. Histochemistry and Cell Biology, 132(5), 491-503. [More Information]

2008

  • Whitelock, J., Melrose, J., Iozzo, R. (2008). Diverse Cell Signaling Events Modulated by Perlecan. Biochemistry, 47(43), 11174-11183. [More Information]
  • Melrose, J., Fuller, E., Roughley, P., Smith, M., Kerr, B., Hughes, C., Caterson, B., Little, C. (2008). Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degenerate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Arthritis Research and Therapy, 10(doi:10.1186/ar2453), 1-10. [More Information]
  • Cake, M., Melrose, J. (2008). Intervertebral disc is an alternate tissue source of circulating C-telopeptide of type II collagen after menopause or ovariectomy: comment on the article by Sondergaard et al. Arthritis and Rheumatism, 58(5), 1560. [More Information]
  • Smith, M., Sakurai, G., Smith, S., Young, A., Melrose, J., Stewart, C., Appleyard, R., Peterson, J., Gilles, R., Dart, A., Sonnabend, D., Little, C. (2008). Modulation of Aggrecan and ADAMTS Expression in Ovine Tendinopathy Induced by Altered Strain. Seminars in Arthritis and Rheumatism, 58(4), 1055-1066. [More Information]
  • Melrose, J., Hayes, A., Whitelock, J., Little, C. (2008). Perlecan, the jack of all trades" proteoglycan of cartilaginous weight-bearing connective tissues.". BioEssays : news and reviews in molecular cellular and developmental biology, 30(5), 457-469. [More Information]
  • Melrose, J., Smith, S., Little, C., Moore, R., Vernon-Roberts, B., Fraser, R. (2008). Recent advances in annular pathobiology provide insights into rim-lesion mediated intervertebral disc degeneration and potential new approaches to annular repair strategies. European Spine Journal, 17(9), 1131-1148. [More Information]
  • Melrose, J., Smith, S., Fuller, E., Young, A., Roughley, P., Dart, A., Little, C. (2008). Spatial and temporal catabolism of the small leucine repeat proteoglycans (SLRPs) in an ovine annular lesion model of experimental intervertebral disc degeneration has structure-function implications. World Forum for Spine Research 2008, Switzerland: AO Foundation.
  • Melrose, J., Smith, S., Smith, M., Little, C. (2008). The use of Histochoice (tmR) for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus. Biotechnic and Histochemistry, 83(1), 47-53. [More Information]
  • Melrose, J., Chuang, C., Whitelock, J. (2008). Tissue engineering of cartilages using biomatrices. Journal of Chemical Technology and Biotechnology, 83, 444-463. [More Information]

2007

  • Melrose, J., Smith, S., Appleyard, R., Little, C. (2007). Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc. European Spine Journal, Published online: 31 October 2007, 1-11. [More Information]
  • Alini, M., Eisenstein, S., Ito, K., Little, C., Kettler, A., Masuda, K., Melrose, J., Ralphs, J., Stokes, I., Wilke, H. (2007). Are animal models useful for studying human disc disorders/degeneration? European Spine Journal, Published online: 14 July 2007, 1-18. [More Information]
  • Melrose, J., Smith, S., Fuller, E., Young, A., Roughley, P., Dart, A., Little, C. (2007). Biglycan and fibromodulin fragmentation correlates with temporal and spatial annular remodelling in experimentally injured ovine intervertebral discs. European Spine Journal, 16(12), 2193-2205. [More Information]
  • Young, A., Appleyard, R., Smith, M., Melrose, J., Little, C. (2007). Dynamic Biomechanics Correlate With Histopathology in Human Tibial Cartilage: A Preliminary Study. Clinical Orthopaedics and Related Research, 462, 212-220. [More Information]

2006

  • Whitelock, J., Melrose, J. (2006). Adhesion of Cells to Biomaterials. In Metin Akay (Eds.), Wiley Encyclopedia of Biomedical Engineering. United States: Wiley-Blackwell Publishing.
  • Smith, M., Sakurai, A., Smith, S., Young, A., Melrose, J., Dart, A., Sonnabend, D., Little, C. (2006). Altered aggrecan and ADAMTS expression in overload-induced tendon pathology. 49th Annual Meeting of the Orthopaedic Research Society, United states: Orthopaedic Research Society.
  • Young, A., McLennan, S., Smith, S., Smith, M., Melrose, J., Cake, M., Flannery, C., Sonnabend, D., Little, C. (2006). Changes in expression and immunolcalization of proteoglycan 4 / lubricin in cartilage in an ovine model of early osteoarthritis. 49th Annual Meeting of the Orthopaedic Research Society, United states: Orthopaedic Research Society.
  • Melrose, J., Whitelock, J. (2006). Extracellular Matrix. In Metin Akay (Eds.), Wiley Encyclopedia of Biomedical Engineering. United States: Wiley-Blackwell Publishing.
  • Young, A., McLennan, S., Smith, M., Smith, S., Cake, M., Read, R., Melrose, J., Sonnabend, D., Flannery, C., Little, C. (2006). Proteoglycan 4 downregulation in a sheep meniscectomy model of early osteoarthritis. Arthritis Research and Therapy, 8(2), 1-6. [More Information]
  • Melrose, J., Roughley, P., Knox, S., Smith, S., Lord, M., Whitelock, J. (2006). The structure, location and function of perlecan, a prominent pericellular proteoglycan of foetal, postnatal and mature hyaline cartilages. Journal of Biological Chemistry, 281(48), 36905-36914. [More Information]

2005

  • Melrose, J., Smith, S., Cake, M., Read, R., Whitelock, J. (2005). Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study. Histochemistry and Cell Biology, 124(3-4), 225-235. [More Information]
  • Young, A., Smith, M., Smith, S., Burkhardt, D., Cake, M., Melrose, J., Read, R., Ghosh, P., Sonnabend, D., Little, C. (2005). Metabolism of Small Leucine-Rich Proteoglycans in an Ovine Model of Osteoarthritis. 51st Annual Meeting of the Orthopaedic Research Society, 30, 1057-1057.
  • Melrose, J., Smith, S., Cake, M., Read, R., Whitelock, J. (2005). Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint. Histochemistry and Cell Biology, 123(6), 561-571. [More Information]
  • Knox, S., Fosang, A., Last, K., Melrose, J., Whitelock, J. (2005). Perlecan from human epithelial cells is a hybrid heparan/chondroitin/keratan sulfate proteoglycan. FEBS Letters, 579(22), 5019-5023. [More Information]
  • Young, A., Smith, M., Smith, S., Cake, M., Ghosh, P., Read, R., Melrose, J., Sonnabend, D., Roughley, P., Little, C. (2005). Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis. Arthritis Research and Therapy, 7(4), R852-61. [More Information]

2004

  • Melrose, J., Smith, S. (2004). Histochemical Visualization Of The Cartilage Hyaladherins Using A Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe. Methods in molecular medicine, 101(1), 65-78.
  • Melrose, J., Smith, S., Ghosh, P. (2004). Histological And Immunohistological Studies On Cartilage. Methods in molecular medicine, 101(1), 39-63.
  • Melrose, J., Smith, S., Whitelock, J. (2004). Perlecan Immunolocalizes To Perichondrial Vessels And Canals In Human Fetal Cartilaginous Primordia In Early Vascular And Matrix Remodeling Events Associated With Diarthrodial Joint Development. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 52(11), 1405-1413.

2003

  • Melrose, J., Smith, S., Ghosh, P. (2003). Assessment of the cellular heterogeneity of the ovine intervertebral disc: comparison with synovial fibroblasts and articular chondrocytes. European Spine Journal, 12(1), 57-65.
  • Shen, B., Melrose, J., Ghosh, P., Taylor, T. (2003). Induction of matrix metalloproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in three-dimensional agarose gel culture by interleukin-1beta: a potential pathway of disc degeneration. European Spine Journal, 12(1), 66-75.
  • Melrose, J., Smith, S., Ghosh, P., Whitelock, J. (2003). Perlecan, the multidomain heparan sulfate proteoglycan of basement membranes, is also a prominent component of the cartilaginous primordia in the developing human fetal spine. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 51(10), 1331-1341.

2002

  • Melrose, J., Roberts, S., Smith, S., Menage, J., Ghosh, P. (2002). Increased Nerve and Blood Vessel Ingrowth Associated With Proteoglycan Depletion in an Ovine Anular Lesion Model of Experimental Disc Degeneration. Spine, 27(12), 1278-1285.
  • Knox, S., Merry, C., Stringer, S., Melrose, J., Whitelock, J. (2002). Not All Perlecans Are Created Equal. Journal of Biological Chemistry, 277(17), 14657-14665.
  • Melrose, J., Smith, S., Knox, S., Whitelock, J. (2002). Perlecan, the multidomain HS-proteoglycan of basement membranes, is a prominent pericellular component of ovine hypertrophic vertebral growth plate and cartilaginous endplate chondrocytes. Histochemistry and Cell Biology, 118(4), 269-280.
  • Melrose, J., Smith, S., Little, C., Kitson, J., Hwa, S., Ghosh, P. (2002). Spatial and temporal localization of transforming growth factor-b, fibroblast growth factor-2, and osteonectin, and identification of cells expressing a-smooth muscle actin in the injured annulus fibrosus. Implications for extracellular matrix repair. Spine, 27(16), 1756-1764.
  • Melrose, J., Smith, S., Ghosh, P. (2002). Synthesis of a Kunitz-Type Serine Proteinase Inhibitory Protein That Shares Homology with Bovine Pancreatic Trypsin Inhibitor by Ovine Intervertebral Disc Cells in Serum-Free alginate Bead Culture. Journal of Spinal Disorders and Techniques, 15(2), 164-171.
  • Melrose, J., Tammi, M., Smith, S. (2002). Visualisation of hyaluronan and hyaluronan-binding proteins within ovine vertebral cartilages using biotinylated aggrecan G1-link complex and biotinylated hyaluronan oligosaccharides. Histochemistry and Cell Biology, 117(4), 327-333. [More Information]

2001

  • Melrose, J., Ghosh, P., Taylor, T. (2001). A comparative analysis of the differential spatial and temporal distributions of the large (aggreca. Journal of Anatomy, 198, 3-15.
  • Melrose, J., Ghosh, P., Taylor, T. (2001). A comparative analysis of the differential spatial and temporal distributions of the large (aggrecan, versican) and small (decorin, biglycan, fibromodulin) proteoglycans of the intervertebral disc. Journal of Anatomy, 198(1), 3-15.
  • Melrose, J., Ghosh, P., Shen, B. (2001). Affinity and western blotting reveal homologies between ovine intervertebral disc serine proteinase. Proteomics, 1, 1529-1533.
  • Melrose, J. (2001). Cartilage and smooth muscle cell proteoglycans detected by affinity blotting using biotinylated hyaluronan. In Iozzo RV (Eds.), Proteoglycan Protocols, (pp. 53-66). United States: Humana Press.
  • Melrose, J., Ghosh, P., Taylor, T., Smith, S. (2001). Differential expression of proteoglycan epitopes and growth characteristics of intervertebral disc. Cells Tissues Organs, 168(3), 137-146.
  • Melrose, J., Knox,, S., Whitelock, J. (2001). Electrophoretic, biosensor, and bioactivity analyses of perlecans of different molecular size. Proteomics, 1, 1534-1541.
  • Melrose, J., Smith, S., Little, C., Kitson,, J., Hwa, S., Ghosh, P. (2001). Repair of annular disc lesions: Spatial and temporal localisation of TGF-beta, SPARC (Osteonectin, BM-40) and FGF-2 are early responses in experimentally injured ovine discs. 47th Annual Meeting of the Orthopaedic Research Society, USA: Orthopaedic Research Society.

2000

  • Taylor, T., Melrose, J., Burkhardt, D., Ghosh, P., Kettler, A., Wilke, H., Claes, L. (2000). Spinal biomechanics and aging are major determinants of the proteoglycan metabolism of intervertebral disc cells. Spine, 25(23), 3014-3020.

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