Dr Jim Manos

Senior Lecturer in Infectious Diseases
Medicine (Immunology & Infectious Diseases), Central Clinical School

Telephone +61 2 9351 8942
Fax +61 2 9351 5319

Map

Biographical details

Dr Jim Manos is Head of the Bacterial Pathogens in Cystic Fibrosis research group in the Sydney Medical School at Sydney University. He is also a Senior Lecturer in Infectious Diseases in the Department of Infectious Diseases and Immunology. He has been studying Pseudomonas aeruginosa strains infecting the cystic fibrosis (CF) lung since 2004. CF-specific strains are a major cause of morbidity and mortality in CF patients. His current work involves elucidation of the pathogenic mechanisms utilised by CF-specific strains of P. aeruginosa, through investigations of changes in transcriptomic expression and genomic composition of acute and chronic infection strains of P. aeruginosa. To this end he has refined an artificial sputum medium (ASMDM) which mimics CF sputum and has enabled detection of novel differentially-expressed genes. Previously Jim worked on Helicobacter pylori and Proteus mirabilis, and has over 20 publications in high quality peer-reviewed journals.

Research interests

Pseudomonas aeruginosa (P.aeruginosa) lung infection is a major cause of morbidity and mortality in cystic fibrosis (CF)patients. P.aeruginosa usually spreads to patients from the environment, but epidemic clones spreading patient-to-patient are an emerging threat to CF patients internationally. Dr Manos is characterising the molecular basis for infectivity of the epidemic clones AES-1 and AES-2 that infect up to 45% of CF patients in eastern Australia, by identifying expression and presence virulence factors specific to AES-1. The findings will be used to develop novel therapeutics and infection control strategies. Data from Affymetrix PAO1 microarrays of AES-1, AES-2 and unique (non-epidemic) isolates grown both planktonically and as a biofilm have provided evidence that epidemic strains are ?primed? for biofilm development during planktonic growth. To further investigate CF-strain specific genes of P. aeruginosa, the CF research group at the University of Sydney, with Dr Manos as a chief investigator, sequenced the genome of AES-1 and in conjuction with Dr Cynthia Whitchurch (UTS - previously Monash) has developed a non-redundant array (PANarray) containing all genes from eight sequenced P. aeruginosa genomes. Dr Manos' lab is using this array, together with an artificial sputum medium (ASMDM) that mimics CF sputum, to investigate expression of CF-strain specific genes. His PhD student Chris Harmer has created mutants in six genes significantly diffrentially expressed in ASMDM, and is investigating changes in virulence, infectivity and invasiveness in the mutants in the A549 epithelial cell line and the C57Bl/6J mouse model of lung infection. Honours student Matthew Wyn is identifying the phenotypic changes that result in these mutants compared to wildtype.

Teaching and supervision

I teach the Infection and Immunity(INIM) postgraduate coursework unit of study "Advanced Medical Bacteriology" (INIM5011) and coordinate the Graduate studies in Infection and Immunity program. The emphasis of the program is to impart the latest information and "state of the art" technology used to understand the specific pathogen under investigation. This theme has been continued in the program of practical experiments that I developed to complement the lectures. I also teach in the BMedSci undergraduate course Infectious Diseases (INFD3012), the INIM unit of study "Global Control of Infectious Diseases" (INIM5022) and in the Graduate Medical Program (GMP).

Selected grants

2010

  • Identification of proteins specific to transmissible Pseudomonas aeruginosa in cystic fibrosis infection; Cordwell S, Manos J, Harbour C; National Health and Medical Research Council (NHMRC)/Project Grants.

2006

  • Sequencing of transmissible Pseudomonas aeruginosa from patients with cystic fibrosis; Harbour C, Rose B, Manos J, Arthur J, Bye P; Australian Cystic Fibrosis Research Trust/Research Support.

Selected publications

Download citations: PDF RTF Endnote

Journals

  • De Soyza, A., Hall, A., Mahenthiralingam, E., Drevinek, P., Kaca, W., Drulis-Kawa, Z., Stoitsova, S., Toth, V., Coenye, T., Zlosnik, J., Manos, J., et al (2013). Developing an international Pseudomonas aeruginosa reference panel. MicrobiologyOpen, 2(6), 1010-1023. [More Information]
  • Harmer, C., Alnassafi, K., Hu, H., Elkins, M., Bye, P., Rose, B., Cordwell, S., Triccas, J., Harbour, C., Manos, J. (2013). Modulation of gene expression by Pseudomonas aeruginosa during chronic infection in the adult cystic fibrosis lung. Microbiology, 159(11), 2354-2363. [More Information]
  • Scott, N., Hare, N., White, M., Manos, J., Cordwell, S. (2013). Secretome of Transmissible Pseudomonas aeruginosa AES-1R Grown in a Cystic Fibrosis Lung-Like Environment. Journal of Proteome Research, 12(12), 5357-5369. [More Information]
  • Hu, H., Harmer, C., Anuj, S., Wainwright, C., Manos, J., Cheney, J., Harbour, C., Zablotska, I., Turnbull, L., Whitchurch, C., Rose, B., et al (2013). Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening. Clinical Microbiology and Infection, 19(3), 266-272. [More Information]
  • Manos, J., Hu, H., Rose, B., Wainwright, C., Zablotska, I., Cheney, J., Turnbull, L., Whitchurch, C., Grimwood, K., Harmer, C., Harbour, C., et al (2013). Virulence factor expression patterns in Pseudomonas aeruginosa strains from infants with cystic fibrosis. European Journal of Clinical Microbiology and Infectious Diseases, 32(12), 1583-1592. [More Information]
  • Hare, N., Solis, N., Harmer, C., Marzook, N., Rose, B., Harbour, C., Crossett, B., Manos, J., Cordwell, S. (2012). Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain. BMC Microbiology, 12(January 2012), 1-14. [More Information]
  • Hare, N., Soe, C., Rose, B., Harbour, C., Codd, R., Manos, J., Cordwell, S. (2012). Proteomics of Pseudomonas aeruginosa Australian Epidemic Strain 1 (AES-1) Cultured under Conditions Mimicking the Cystic Fibrosis Lung Reveals Increased Iron Acquisition via the Siderophore Pyochelin. Journal of Proteome Research, 11(2), 776-795. [More Information]
  • Harmer, C., Triccas, J., Hu, H., Rose, B., Bye, P., Elkins, M., Manos, J. (2012). Pseudomonas aeruginosa strains from the chronically infected cystic fibrosis lung display increased invasiveness of A549 epithelial cells over time. Microbial Pathogenesis, 53(1), 37-43. [More Information]
  • Naughton, S., Parker, D., Seemann, T., Thomas, T., Turnbull, L., Rose, B., Bye, P., Cordwell, S., Whitchurch, C., Manos, J. (2011). Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung. PLoS One, 6(9), e24526-1-e24526-8. [More Information]
  • Tingpej, P., Elkins, M., Rose, B., Hu, H., Moriarty, C., Manos, J., Barras, B., Bye, P., Harbour, C. (2010). Clinical profile of adult cystic fibrosis patients with frequent epidemic clones of Pseudomonas aeruginosa. Respirology, 15(6), 923-929. [More Information]
  • Fung, C., Naughton, S., Turnbull, L., Tingpej, P., Rose, B., Arthur, J., Hu, H., Harmer, C., Harbour, C., Hassett, D., Manos, J., et al (2010). Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking CF lung sputum. Journal of Medical Microbiology, 59(Pt9), 1089-1100. [More Information]
  • Manos, J., Arthur, J., Rose, B., Bell, S., Tingpej, P., Hu, H., Webb, J., Kjelleberg, S., Gorrell, M., Bye, P., Harbour, C. (2009). Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth. FEMS Microbiology Letters, 292(1), 107-114. [More Information]
  • Manos, J., Arthur, J., Rose, B., Tingpej, P., Fung, C., Curtis, M., Webb, J., Hu, H., Kjelleberg, S., Gorrell, M., Bye, P., Harbour, C. (2008). Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung. Journal of Medical Microbiology, 57(12), 1454-1465. [More Information]
  • Tingpej, P., Smith, L., Rose, B., Zhu, H., Conibear, T., Al Nassafi, K., Manos, J., Elkins, M., Bye, P., Willcox, M., Harbour, C., et al (2007). Phenotypic characterization of clonal and nonclonal Pseudomonas aeruginosa strains isolated from lungs of adults with cystic fibrosis. Journal of Clinical Microbiology, 45(6), 1697-1704. [More Information]
  • Smith, L., Rose, B., Tingpej, P., Zhu, H., Conibear, T., Manos, J., Bye, P., Elkins, M., Willcox, M., Bell, S., Harbour, C., et al (2006). Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis. Journal of Medical Microbiology, 55(Pt 12), 1641-1644. [More Information]
  • Manos, J., Artimovich, E., Belas, R. (2004). Enhanced motility of a Proteus mirabilis strain expressing hybrid FlaAB flagella. Microbiology, 150(5), 1291-1299.
  • Belas, R., Manos, J., Suvanasuthi, R. (2004). Proteus mirabilis ZapA Metalloprotease Degrades a Broad Spectrum of Substrates, Including Antimicrobial Peptides. Infection and Immunity, 72(9), 5159-5167.
  • Manos, J., Belas, R. (2004). Transcription of Proteus mirabilis flaAB. Microbiology, 150(9), 2857-2863.
  • Zhang, D., Manos, J., Ma, X., Belas, R., Karaolis, D. (2004). Transcriptional analysis and operon structure of the tagA-orf2-orf3-mop-tagD region on the Vibrio pathogenicity island in epidemic V. cholerae. FEMS Microbiology Letters, 235(1), 199-207. [More Information]
  • Prouty, A., Brodsky, I., Manos, J., Belas, R., Falkow, S., Gunn, J. (2004). Transcriptional regulation of Salmonella enterica serovar Typhimurium genes by bile. FEMS Immunology and Medical Microbiology, 41(2), 177-185. [More Information]

Other

  • Manos, J., Arthur, J., Rose, B., Harbour, C. (2006), Assessment of infectious bacteria.

2013

  • De Soyza, A., Hall, A., Mahenthiralingam, E., Drevinek, P., Kaca, W., Drulis-Kawa, Z., Stoitsova, S., Toth, V., Coenye, T., Zlosnik, J., Manos, J., et al (2013). Developing an international Pseudomonas aeruginosa reference panel. MicrobiologyOpen, 2(6), 1010-1023. [More Information]
  • Harmer, C., Alnassafi, K., Hu, H., Elkins, M., Bye, P., Rose, B., Cordwell, S., Triccas, J., Harbour, C., Manos, J. (2013). Modulation of gene expression by Pseudomonas aeruginosa during chronic infection in the adult cystic fibrosis lung. Microbiology, 159(11), 2354-2363. [More Information]
  • Scott, N., Hare, N., White, M., Manos, J., Cordwell, S. (2013). Secretome of Transmissible Pseudomonas aeruginosa AES-1R Grown in a Cystic Fibrosis Lung-Like Environment. Journal of Proteome Research, 12(12), 5357-5369. [More Information]
  • Hu, H., Harmer, C., Anuj, S., Wainwright, C., Manos, J., Cheney, J., Harbour, C., Zablotska, I., Turnbull, L., Whitchurch, C., Rose, B., et al (2013). Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening. Clinical Microbiology and Infection, 19(3), 266-272. [More Information]
  • Manos, J., Hu, H., Rose, B., Wainwright, C., Zablotska, I., Cheney, J., Turnbull, L., Whitchurch, C., Grimwood, K., Harmer, C., Harbour, C., et al (2013). Virulence factor expression patterns in Pseudomonas aeruginosa strains from infants with cystic fibrosis. European Journal of Clinical Microbiology and Infectious Diseases, 32(12), 1583-1592. [More Information]

2012

  • Hare, N., Solis, N., Harmer, C., Marzook, N., Rose, B., Harbour, C., Crossett, B., Manos, J., Cordwell, S. (2012). Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain. BMC Microbiology, 12(January 2012), 1-14. [More Information]
  • Hare, N., Soe, C., Rose, B., Harbour, C., Codd, R., Manos, J., Cordwell, S. (2012). Proteomics of Pseudomonas aeruginosa Australian Epidemic Strain 1 (AES-1) Cultured under Conditions Mimicking the Cystic Fibrosis Lung Reveals Increased Iron Acquisition via the Siderophore Pyochelin. Journal of Proteome Research, 11(2), 776-795. [More Information]
  • Harmer, C., Triccas, J., Hu, H., Rose, B., Bye, P., Elkins, M., Manos, J. (2012). Pseudomonas aeruginosa strains from the chronically infected cystic fibrosis lung display increased invasiveness of A549 epithelial cells over time. Microbial Pathogenesis, 53(1), 37-43. [More Information]

2011

  • Naughton, S., Parker, D., Seemann, T., Thomas, T., Turnbull, L., Rose, B., Bye, P., Cordwell, S., Whitchurch, C., Manos, J. (2011). Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung. PLoS One, 6(9), e24526-1-e24526-8. [More Information]

2010

  • Tingpej, P., Elkins, M., Rose, B., Hu, H., Moriarty, C., Manos, J., Barras, B., Bye, P., Harbour, C. (2010). Clinical profile of adult cystic fibrosis patients with frequent epidemic clones of Pseudomonas aeruginosa. Respirology, 15(6), 923-929. [More Information]
  • Fung, C., Naughton, S., Turnbull, L., Tingpej, P., Rose, B., Arthur, J., Hu, H., Harmer, C., Harbour, C., Hassett, D., Manos, J., et al (2010). Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking CF lung sputum. Journal of Medical Microbiology, 59(Pt9), 1089-1100. [More Information]

2009

  • Manos, J., Arthur, J., Rose, B., Bell, S., Tingpej, P., Hu, H., Webb, J., Kjelleberg, S., Gorrell, M., Bye, P., Harbour, C. (2009). Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth. FEMS Microbiology Letters, 292(1), 107-114. [More Information]

2008

  • Manos, J., Arthur, J., Rose, B., Tingpej, P., Fung, C., Curtis, M., Webb, J., Hu, H., Kjelleberg, S., Gorrell, M., Bye, P., Harbour, C. (2008). Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung. Journal of Medical Microbiology, 57(12), 1454-1465. [More Information]

2007

  • Tingpej, P., Smith, L., Rose, B., Zhu, H., Conibear, T., Al Nassafi, K., Manos, J., Elkins, M., Bye, P., Willcox, M., Harbour, C., et al (2007). Phenotypic characterization of clonal and nonclonal Pseudomonas aeruginosa strains isolated from lungs of adults with cystic fibrosis. Journal of Clinical Microbiology, 45(6), 1697-1704. [More Information]

2006

  • Manos, J., Arthur, J., Rose, B., Harbour, C. (2006), Assessment of infectious bacteria.
  • Smith, L., Rose, B., Tingpej, P., Zhu, H., Conibear, T., Manos, J., Bye, P., Elkins, M., Willcox, M., Bell, S., Harbour, C., et al (2006). Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis. Journal of Medical Microbiology, 55(Pt 12), 1641-1644. [More Information]

2004

  • Manos, J., Artimovich, E., Belas, R. (2004). Enhanced motility of a Proteus mirabilis strain expressing hybrid FlaAB flagella. Microbiology, 150(5), 1291-1299.
  • Belas, R., Manos, J., Suvanasuthi, R. (2004). Proteus mirabilis ZapA Metalloprotease Degrades a Broad Spectrum of Substrates, Including Antimicrobial Peptides. Infection and Immunity, 72(9), 5159-5167.
  • Manos, J., Belas, R. (2004). Transcription of Proteus mirabilis flaAB. Microbiology, 150(9), 2857-2863.
  • Zhang, D., Manos, J., Ma, X., Belas, R., Karaolis, D. (2004). Transcriptional analysis and operon structure of the tagA-orf2-orf3-mop-tagD region on the Vibrio pathogenicity island in epidemic V. cholerae. FEMS Microbiology Letters, 235(1), 199-207. [More Information]
  • Prouty, A., Brodsky, I., Manos, J., Belas, R., Falkow, S., Gunn, J. (2004). Transcriptional regulation of Salmonella enterica serovar Typhimurium genes by bile. FEMS Immunology and Medical Microbiology, 41(2), 177-185. [More Information]

To update your profile click here. For support on your academic profile contact .