Dr Leszek Lisowski

Conjoint Senior Lecturer
Children's Medical Research Institute (CMRI)


Map

Selected grants

2018

  • Novel vector selection method for improved functionality; Lisowski L; DVC Research/Bridging Support Grant.
  • DGM70 Single-sided Individually Ventilated Cage (IVC) racks, associated cages, air handling unit and change station - equipment for a human xenograft mouse facility; Alexander I, Lisowski L, Reddel R; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Retinal gene therapy delivery: best route, right place; Lisowski L, Jamieson R, Grigg J, Grunert U, Madigan M; Ophthalmic Research Institute of Australia (ORIA)/Research Grant.

2017

  • Sydney Vision Initiative; Jamieson R, McCluskey P, Lisowski L, Alexander I, Grigg J, Tam P, Schofield D, Simunovic M; DVC Research/Sydney Research Excellence Initiative 2020 (SREI).

2016

  • Directed evolution of AAV capsid variants for enhanced targeted genome editing in the human liver; Lisowski L, Alexander I, Tse H; National Health and Medical Research Council (NHMRC)/Project Grants.

Selected publications

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Book Chapters

  • Diecke, S., Lisowski, L., Kooreman, N., Wu, J. (2014). Second Generation Codon Optimized Minicircle (CoMiC) for Nonviral Reprogramming of Human Adult Fibroblasts. In Milica Radisic and Lauren D. Black III (Eds.), Methods in Molecular Biology 1181; Cardiac Tissue Engineering Methods and Protocols, (pp. 1-13). New York Heidelberg Dordrecht London: Springer. [More Information]

Journals

  • Roediger, B., Lee, Q., Tikoo, S., Cobbin, J., Henderson, J., Jormakka, M., O'Rourke, M., Padula, M., Pinello, N., Henry, M., Wynne, M., Lisowski, L., Tay, S., Harris, D., Bertolino, P., Wong, J., Gorrell, M., Holmes, E., Jolly, C., Weninger, W., et al (2018). An Atypical Parvovirus Drives Chronic Tubulointerstitial Nephropathy and Kidney Fibrosis. Cell, In Press, 1-39. [More Information]
  • Paulk, N., Pekrun, K., Zhu, E., Nygaard, S., Li, B., Xu, J., Chu, K., Leborgne, C., Dane, A., Haft, A., Alexander, I., Lisowski, L. (2018). Bioengineered AAV Capsids with Combined High Human Liver Transduction In�Vivo and Unique Humoral Seroreactivity. Molecular Therapy, 26(1), 289-303. [More Information]
  • Paulk, N., Pekrun, K., Charville, G., Maguire-Nguyen, K., Wosczyna, M., Xu, J., Zhang, Y., Lisowski, L., Yoo, B., Vilches-Moure, J., et al (2018). Bioengineered Viral Platform for Intramuscular Passive Vaccine Delivery to Human Skeletal Muscle. Molecular Therapy - Methods & Clinical Development, 10, 144-155. [More Information]
  • Moreno, A., Fu, X., Zhu, J., Katrekar, D., Shih, Y., Marlett, J., Cabotaje, J., Tat, J., Naughton, J., Lisowski, L., et al (2018). In Situ Gene Therapy via AAV-CRISPR-Cas9-Mediated Targeted Gene Regulation. Molecular Therapy, 26(7), 1818-1827. [More Information]
  • Logan, G., Dane, A., Hallwirth, C., Smyth, C., Wilkie, E., Amaya, A., Zhu, E., Khandekar, N., Ginn, S., Liao, S., Cunningham, S., Sasaki, N., Cabanes-Creus, M., Tam, P., Lisowski, L., Alexander, I., et al (2017). Identification of liver-specific enhancer-promoter activity in the 3' untranslated region of the wild-type AAV2 genome. Nature Genetics, 49(8), 1267-1273. [More Information]
  • Winter, I., Chiou, S., Paulk, N., McFarland, C., Lalgudi, P., Ma, R., Lisowski, L., Connolly, A., Petrov, D., Kay, M., et al (2017). Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity. Nature Communications, 8(1), 1-16. [More Information]
  • Valdmanis, P., Gu, S., Chu, K., Jin, L., Zhang, F., Munding, E., Zhang, Y., Huang, Y., Kutay, H., Lisowski, L., et al (2016). RNA interference-induced hepatotoxicity results from loss of the first synthesized isoform of microRNA-122 in mice. Nature Medicine, 22(5), 557-562. [More Information]
  • Lisowski, L., Tay, S., Alexander, I. (2015). Adeno-associated virus serotypes for gene therapeutics. Current Opinion in Pharmacology, 24, 59-67. [More Information]
  • Wang, Y., Liang, P., Lan, F., Wu, H., Lisowski, L., Gu, M., Hu, S., Kay, M., Urnov, F., Shinnawi, R., et al (2014). Genome editing of isogenic human induced pluripotent stem cells recapitulates long QT phenotype for drug testing. Journal of the American College of Cardiology, 64(5), 451-459. [More Information]
  • Sebastiano, V., Zhen, H., Haddad, B., Bashkirova, E., Melo, S., Wang, P., Leung, T., Siprashvili, Z., Tichy, A., Li, J., Lisowski, L., et al (2014). Human COL7A1-corrected induced pluripotent stem cells for the treatment of recessive dystrophic epidermolysis bullosa. Science Translational Medicine, 6(264), 1-23. [More Information]
  • Lisowski, L., Dane, A., Chu, K., Zhang, Y., Cunningham, S., Wilson, E., Nygaard, S., Grompe, M., Alexander, I., Kay, M. (2014). Selection and evaluation of clinically relevant AAV variants in a xenograft liver model. Nature, 506(7488), 382-386. [More Information]
  • Melo, S., Lisowski, L., Bashkirova, E., Zhen, H., Chu, K., Keene, D., Marinkovich, M., Kay, M., Oro, A. (2014). Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Molecular Therapy, 22(4), 725-733. [More Information]
  • Wang, N., Rajasekaran, N., Hou, T., Lisowski, L., Mellins, E. (2013). Comparison of transduction efficiency among various lentiviruses containing GFP reporter in bone marrow hematopoietic stem cell transplantation. Experimental Hematology, 41(11), 934-943. [More Information]
  • Lisowski, L., Elazar, M., Chu, K., Glenn, J., Kay, M. (2013). The anti-genomic (negative) strand of Hepatitis C Virus is not targetable by shRNA. Nucleic Acids Research, 41(6), 3688-3698. [More Information]
  • Wang, Z., Lisowski, L., Finegold, M., Nakai, H., Kay, M., Grompe, M. (2012). AAV vectors containing rDNA homology display increased chromosomal integration and transgene persistence. Molecular Therapy, 20(10), 1902-1911. [More Information]
  • Wang, Y., Zhang, W., Hu, S., Lan, F., Lee, A., Huber, B., Lisowski, L., Liang, P., Huang, M. (2012). Genome editing of human embryonic stem cells and induced pluripotent stem cells with zinc finger nucleases for cellular imaging. Circulation Research, 111(12), 1494-1503. [More Information]
  • Valdmanis, P., Lisowski, L., Kay, M. (2012). rAAV-mediated tumorigenesis: still unresolved after an AAV assault. Molecular Therapy, 20(11), 2014-2017. [More Information]
  • Lisowski, L., Lau, A., Wang, Z., Zhang, Y., Zhang, F., Grompe, M., Kay, M. (2012). Ribosomal DNA integrating rAAV-rDNA vectors allow for stable transgene expression. Molecular Therapy, 20(10), 1912-1923. [More Information]

2018

  • Roediger, B., Lee, Q., Tikoo, S., Cobbin, J., Henderson, J., Jormakka, M., O'Rourke, M., Padula, M., Pinello, N., Henry, M., Wynne, M., Lisowski, L., Tay, S., Harris, D., Bertolino, P., Wong, J., Gorrell, M., Holmes, E., Jolly, C., Weninger, W., et al (2018). An Atypical Parvovirus Drives Chronic Tubulointerstitial Nephropathy and Kidney Fibrosis. Cell, In Press, 1-39. [More Information]
  • Paulk, N., Pekrun, K., Zhu, E., Nygaard, S., Li, B., Xu, J., Chu, K., Leborgne, C., Dane, A., Haft, A., Alexander, I., Lisowski, L. (2018). Bioengineered AAV Capsids with Combined High Human Liver Transduction In�Vivo and Unique Humoral Seroreactivity. Molecular Therapy, 26(1), 289-303. [More Information]
  • Paulk, N., Pekrun, K., Charville, G., Maguire-Nguyen, K., Wosczyna, M., Xu, J., Zhang, Y., Lisowski, L., Yoo, B., Vilches-Moure, J., et al (2018). Bioengineered Viral Platform for Intramuscular Passive Vaccine Delivery to Human Skeletal Muscle. Molecular Therapy - Methods & Clinical Development, 10, 144-155. [More Information]
  • Moreno, A., Fu, X., Zhu, J., Katrekar, D., Shih, Y., Marlett, J., Cabotaje, J., Tat, J., Naughton, J., Lisowski, L., et al (2018). In Situ Gene Therapy via AAV-CRISPR-Cas9-Mediated Targeted Gene Regulation. Molecular Therapy, 26(7), 1818-1827. [More Information]

2017

  • Logan, G., Dane, A., Hallwirth, C., Smyth, C., Wilkie, E., Amaya, A., Zhu, E., Khandekar, N., Ginn, S., Liao, S., Cunningham, S., Sasaki, N., Cabanes-Creus, M., Tam, P., Lisowski, L., Alexander, I., et al (2017). Identification of liver-specific enhancer-promoter activity in the 3' untranslated region of the wild-type AAV2 genome. Nature Genetics, 49(8), 1267-1273. [More Information]
  • Winter, I., Chiou, S., Paulk, N., McFarland, C., Lalgudi, P., Ma, R., Lisowski, L., Connolly, A., Petrov, D., Kay, M., et al (2017). Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity. Nature Communications, 8(1), 1-16. [More Information]

2016

  • Valdmanis, P., Gu, S., Chu, K., Jin, L., Zhang, F., Munding, E., Zhang, Y., Huang, Y., Kutay, H., Lisowski, L., et al (2016). RNA interference-induced hepatotoxicity results from loss of the first synthesized isoform of microRNA-122 in mice. Nature Medicine, 22(5), 557-562. [More Information]

2015

  • Lisowski, L., Tay, S., Alexander, I. (2015). Adeno-associated virus serotypes for gene therapeutics. Current Opinion in Pharmacology, 24, 59-67. [More Information]

2014

  • Wang, Y., Liang, P., Lan, F., Wu, H., Lisowski, L., Gu, M., Hu, S., Kay, M., Urnov, F., Shinnawi, R., et al (2014). Genome editing of isogenic human induced pluripotent stem cells recapitulates long QT phenotype for drug testing. Journal of the American College of Cardiology, 64(5), 451-459. [More Information]
  • Sebastiano, V., Zhen, H., Haddad, B., Bashkirova, E., Melo, S., Wang, P., Leung, T., Siprashvili, Z., Tichy, A., Li, J., Lisowski, L., et al (2014). Human COL7A1-corrected induced pluripotent stem cells for the treatment of recessive dystrophic epidermolysis bullosa. Science Translational Medicine, 6(264), 1-23. [More Information]
  • Diecke, S., Lisowski, L., Kooreman, N., Wu, J. (2014). Second Generation Codon Optimized Minicircle (CoMiC) for Nonviral Reprogramming of Human Adult Fibroblasts. In Milica Radisic and Lauren D. Black III (Eds.), Methods in Molecular Biology 1181; Cardiac Tissue Engineering Methods and Protocols, (pp. 1-13). New York Heidelberg Dordrecht London: Springer. [More Information]
  • Lisowski, L., Dane, A., Chu, K., Zhang, Y., Cunningham, S., Wilson, E., Nygaard, S., Grompe, M., Alexander, I., Kay, M. (2014). Selection and evaluation of clinically relevant AAV variants in a xenograft liver model. Nature, 506(7488), 382-386. [More Information]
  • Melo, S., Lisowski, L., Bashkirova, E., Zhen, H., Chu, K., Keene, D., Marinkovich, M., Kay, M., Oro, A. (2014). Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Molecular Therapy, 22(4), 725-733. [More Information]

2013

  • Wang, N., Rajasekaran, N., Hou, T., Lisowski, L., Mellins, E. (2013). Comparison of transduction efficiency among various lentiviruses containing GFP reporter in bone marrow hematopoietic stem cell transplantation. Experimental Hematology, 41(11), 934-943. [More Information]
  • Lisowski, L., Elazar, M., Chu, K., Glenn, J., Kay, M. (2013). The anti-genomic (negative) strand of Hepatitis C Virus is not targetable by shRNA. Nucleic Acids Research, 41(6), 3688-3698. [More Information]

2012

  • Wang, Z., Lisowski, L., Finegold, M., Nakai, H., Kay, M., Grompe, M. (2012). AAV vectors containing rDNA homology display increased chromosomal integration and transgene persistence. Molecular Therapy, 20(10), 1902-1911. [More Information]
  • Wang, Y., Zhang, W., Hu, S., Lan, F., Lee, A., Huber, B., Lisowski, L., Liang, P., Huang, M. (2012). Genome editing of human embryonic stem cells and induced pluripotent stem cells with zinc finger nucleases for cellular imaging. Circulation Research, 111(12), 1494-1503. [More Information]
  • Valdmanis, P., Lisowski, L., Kay, M. (2012). rAAV-mediated tumorigenesis: still unresolved after an AAV assault. Molecular Therapy, 20(11), 2014-2017. [More Information]
  • Lisowski, L., Lau, A., Wang, Z., Zhang, Y., Zhang, F., Grompe, M., Kay, M. (2012). Ribosomal DNA integrating rAAV-rDNA vectors allow for stable transgene expression. Molecular Therapy, 20(10), 1912-1923. [More Information]

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