Dr Mark Graham

Senior Lecturer
Medicine, Children's Medical Research Institute

Telephone +61 2 8865 2989
Fax +61 2 9687 2120

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Research interests

Dr Graham’s overall aim is to understand the molecular basis of neurological diseases. In particular, his group is focusing on proteins involved in neurotransmission, how they interact, mapping cellular signalling events triggered by neurotransmission and identifying regulatory mechanisms that could potentially be exploited by therapeutics for diseases of neurotransmission, e.g. epilepsy, autism, schizophrenia, bipolar disorder, etc. The biogenesis of synaptic vesicles via endocytosis is important for neurotransmission and is also a focus for Dr Graham. His group is determining the mechanisms of how vesicles are made by investigation of the protein complex that forms a clathrin coat over budding vesicles (a complex of clathrin, the adapter protein complex 2 and assembly protein AP180). Dr Graham uses approaches at the interface of chemistry and biology. A major tool is the use of both targeted and global proteomics to expand knowledge on protein interactions and signalling mechanisms.

Selected grants

2014

  • Aberrant mesenchymal-epithelial transition: a pathogenic mechanism in tissue maintenance and differentiation; Tam P, Loebel D, Graham M, Arthur J; National Health and Medical Research Council (NHMRC)/Project Grants.

2013

  • Phosphorylation of synaptic vesicle glycoprotein 2 (SV2) regulates endocytosis of synaptotagmin for synaptic transmission; Robinson P, Graham M; National Health and Medical Research Council (NHMRC)/Project Grants.

2012

  • Role of oxidative stress in the human genetic disorder ataxia telangiectasia; Graham M, Lavin M; National Health and Medical Research Council (NHMRC)/Project Grants.
  • A Pilot Study of Pre-synaptic Protein Kinases; Graham M, Robinson P; DVC Research/Bridging Support Grant.

2011

  • Identification of the phospho-regulated synaptic vesicle endocytosis machinery; Graham M, Robinson P; Rebecca L Cooper Medical Research Foundation/Equipment Grant.

2009

  • Inhibitory signaling switches define the machinery of synaptic vesicle endocytosis; Graham M; National Health and Medical Research Council (NHMRC)/Career Development Awards.
  • Mechanisms of synaptic vesicle endocytosis revealed by its regulatory phosphoproteome; Robinson P, Graham M; National Health and Medical Research Council (NHMRC)/Project Grants.
  • O-GlcNAc-phosphorylation: a novel post-translational modification regulating vesicle recycling.; Graham M, Robinson P; Australian Research Council (ARC)/Discovery Projects (DP).
  • Inhibitory signaling switches define the machinery of synaptic vesicle endocytosis; Graham M; DVC Research/Research Fellow Establishment Grants.

Selected publications

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Journals

  • Moshkanbaryans, L., Chan, L., Graham, M. (2014). The Biochemical Properties and Functions of CALM and AP180 in Clathrin Mediated Endocytosis. Membranes, 4(3), 388-413. [More Information]
  • Parker, B., Thaysen-Andersen, M., Solis, N., Scott, N., Larsen, M., Graham, M., Packer, N., Cordwell, S. (2013). Site-Specific Glycan-Peptide Analysis for Determination of N-Glycoproteome Heterogeneity. Journal of Proteome Research, 12(12), 5791-8000. [More Information]
  • Palmisano, G., Parker, B., Engholm-Keller, K., Lendal, S., Kulej, K., Schulz, M., Schwammle, V., Graham, M., Saxtorph, H., Cordwell, S., et al (2012). A novel method for the simultaneous enrichment, identification, and quantification of phosphopeptides and sialylated glycopeptides applied to a temporal profile of mouse brain development. Molecular and Cellular Proteomics, 11(11), 1191-1202. [More Information]
  • Bolderson, E., Savage, K., Mahen, R., Pisupati, V., Graham, M., Richard, D., Robinson, P., Venkitaraman, A., Khanna, K. (2012). Kruppel-associated Box (KRAB)-associated co-repressor (KAP-1) Ser-473 phosphorylation regulates heterochromatin protein 1B (HP1-B) mobilization and DNA repair in heterochromatin. Journal of Biological Chemistry, 287(33), 28122-28131. [More Information]
  • Quan, A., Xue, J., Wielens, J., Smillie, K., Anggono, V., Parker, M., Cousin, M., Graham, M., Robinson, P. (2012). Phosphorylation of syndapin I F-BAR domain at two helix-capping motifs regulates membrane tubulation. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 109(10), 3760-3765. [More Information]
  • Graham, M., Thaysen-Andersen, M., Bache, N., Craft, G., Larsen, M., Packer, N., Robinson, P. (2011). A Novel Post-translational Modification in Nerve Terminals: O-Linked N-Acetylglucosamine Phosphorylation. Journal of Proteome Research, 10(6), 2725-2733. [More Information]
  • Kozlov, S., Graham, M., Jakob, B., Tobias, F., Kijas, A., Tanuji, M., Chen, P., Robinson, P., Taucher-Scholz, G., Suzuki, K., et al (2011). Autophosphorylation and ATM activation: additional sites add to the complexity. Journal of Biological Chemistry, 286(11), 9107-9119. [More Information]
  • Xue, J., Graham, M., Novelle, A., Sue, N., Gray, N., McNiven, M., Smillie, K., Cousin, M., Robinson, P. (2011). Calcineurin Selectively Docks with the Dynamin Ixb Splice Variant to Regulate Activity-dependent Bulk Endocytosis. Journal of Biological Chemistry, 286(35), 30295-30303. [More Information]
  • Chircop (nee Fabbro), M., Sarcevic, B., Larsen, M., Malladi, C., Chau, N., Zavortink, M., Smith, C., Quan, A., Anggono, V., Hains, P., Graham, M., Robinson, P. (2011). Phosphorylation of dynamin II at serine-764 is associated with cytokinesis. BBA: Biochimica et Biophysica Acta - Molecular Cell Research, 1813 (10), 1689-1699. [More Information]
  • Chan, L., Hansra, G., Robinson, P., Graham, M. (2010). Differential Phosphorylation of Dynamin I Isoforms in Subcellular Compartments Demonstrates the Hidden Complexity of Phosphoproteomes. Journal of Proteome Research, 9(8), 4028-4037. [More Information]
  • Baker, M., Smith, N., Hetherington, L., Taubman, K., Graham, M., Robinson, P., Aitken, R. (2010). Label-Free Quantitation of Phosphopeptide Changes During Rat Sperm Capacitation. Journal of Proteome Research, 9(2), 718-729. [More Information]
  • Odell, L., Chau, N., Mariana, A., Graham, M., Robinson, P., McCluskey, A. (2009). Azido and Diazarinyl Analogues of Bis-Tyrphostin as Asymmetrical Inhibitors of Dynamin GTPase. ChemMedChem: chemistry enabling drug discovery, 4(7), 1182-1188.
  • Chircop (nee Fabbro), M., Oakes, V., Graham, M., Ma, M., Smith, C., Robinson, P., Khanna, K. (2009). The actin-binding and bundling protein, EPLIN, is required for cytokinesis. Cell Cycle, 8(5), 757-764. [More Information]
  • Craft, G., Graham, M., Bache, N., Larsen, M., Robinson, P. (2008). The in vivo phosphorylation sites in multiple isoforms of amphiphysin I from rat brain nerve terminals. Molecular and Cellular Proteomics, 7(6), 1146-1161. [More Information]
  • Cohen, S., Graham, M., Lovrecz, G., Bache, N., Robinson, P., Reddel, R. (2007). Protein composition of catalytically active human telomerase from immortal cells. Science, 315(5820), 1850-1853. [More Information]
  • Oganesian, L., Graham, M., Robinson, P., Bryan, T. (2007). Telomerase Recognizes G-Quadruplex and Linear DNA as Distinct Substrates. Biochemistry, 46(40), 11279-11290. [More Information]
  • Graham, M., Kilby, D., Firth, S., Robinson, P., Baxter, R. (2007). The in vivo phosphorylation and glycosylation of human insulin-like growth factor binding protein-5. Molecular and Cellular Proteomics, 6(8), 1392-1405. [More Information]
  • Graham, M., Anggono, V., Bache, N., Larsen, M., Craft, G., Robinson, P. (2007). The in vivo phosphorylation sites of rat brain dynamin I. Journal of Biological Chemistry, 282(20), 14695-14707. [More Information]
  • Acevedo, K., Li, R., Soo, P., Suryadinata, R., Sarcevic, B., Valova, V., Graham, M., Robinson, P., Bernard, O. (2007). The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules. Experimental Cell Research: emphasizing molecular approaches to cell biology, 313(20), 4091-4106. [More Information]
  • Kozlov, S., Graham, M., Peng, C., Chen, P., Robinson, P., Lavin, M. (2006). Involvement of novel autophosphorylation sites in ATM activation. The EMBO Journal, 25(15), 3504-3514.
  • Anggono, V., Smillie, K., Graham, M., Valova, V., Cousin, M., Robinson, P. (2006). Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. Nature Neuroscience, 9(6), 752-760. [More Information]
  • Chircop (nee Fabbro), M., Zhou, B., Takahashi, M., Sarcevic, B., Lal, P., Graham, M., Gabrielli, B., Robinson, P., Nigg, E., Ono, Y., et al (2005). Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Developmental Cell, 9(4), 477-488. [More Information]
  • Hill, T., Odell, L., Edwards, J., Graham, M., McGeachie, A., Rusak, J., Quan, A., Abagyan, R., Scott, J., Robinson, P., et al (2005). Small molecule inhibitors of dynamin I GTPase activity: Development of dimeric tyrphostins. Journal of Medicinal Chemistry, 48(24), 7781-7788.
  • Larsen, M., Graham, M., Robinson, P., Roepstorff, P. (2004). Improved Detection Of Hydrophilic Phosphopeptides Using Graphite Powder Microcolumns And Mass Spectrometry: Evidence For In Vivo Doubly Phosphorylated Dynamin I And Dynamin Iii. Molecular and Cellular Proteomics, 3(5), 456-465.
  • Graham, M., Ruma-Haynes, P., Capes-Davis, A., Dunn, J., Tan, T., Valova, V., Robinson, P., Jeffrey, P. (2004). Multisite Phosphorylation Of Doublecortin By Cyclin-Dependent Kinase 5. Biochemical Journal, 381(2), 471-481.
  • Xue, J., Milburn, P., Hanna, B., Graham, M., Rostas, J., Robinson, P. (2004). Phosphorylation Of Septin 3 On Ser-91 By Cgmp-Dependent Protein Kinase-I In Nerve Terminals. Biochemical Journal, 381(3), 753-760.
  • Tan, T., Valova, V., Malladi, C., Graham, M., Berven, L., Jupp, O., Hansra, G., McClure, S., Sarcevic, B., Boadle, R., Robinson, P., et al (2003). Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), 701-710.

2014

  • Moshkanbaryans, L., Chan, L., Graham, M. (2014). The Biochemical Properties and Functions of CALM and AP180 in Clathrin Mediated Endocytosis. Membranes, 4(3), 388-413. [More Information]

2013

  • Parker, B., Thaysen-Andersen, M., Solis, N., Scott, N., Larsen, M., Graham, M., Packer, N., Cordwell, S. (2013). Site-Specific Glycan-Peptide Analysis for Determination of N-Glycoproteome Heterogeneity. Journal of Proteome Research, 12(12), 5791-8000. [More Information]

2012

  • Palmisano, G., Parker, B., Engholm-Keller, K., Lendal, S., Kulej, K., Schulz, M., Schwammle, V., Graham, M., Saxtorph, H., Cordwell, S., et al (2012). A novel method for the simultaneous enrichment, identification, and quantification of phosphopeptides and sialylated glycopeptides applied to a temporal profile of mouse brain development. Molecular and Cellular Proteomics, 11(11), 1191-1202. [More Information]
  • Bolderson, E., Savage, K., Mahen, R., Pisupati, V., Graham, M., Richard, D., Robinson, P., Venkitaraman, A., Khanna, K. (2012). Kruppel-associated Box (KRAB)-associated co-repressor (KAP-1) Ser-473 phosphorylation regulates heterochromatin protein 1B (HP1-B) mobilization and DNA repair in heterochromatin. Journal of Biological Chemistry, 287(33), 28122-28131. [More Information]
  • Quan, A., Xue, J., Wielens, J., Smillie, K., Anggono, V., Parker, M., Cousin, M., Graham, M., Robinson, P. (2012). Phosphorylation of syndapin I F-BAR domain at two helix-capping motifs regulates membrane tubulation. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 109(10), 3760-3765. [More Information]

2011

  • Graham, M., Thaysen-Andersen, M., Bache, N., Craft, G., Larsen, M., Packer, N., Robinson, P. (2011). A Novel Post-translational Modification in Nerve Terminals: O-Linked N-Acetylglucosamine Phosphorylation. Journal of Proteome Research, 10(6), 2725-2733. [More Information]
  • Kozlov, S., Graham, M., Jakob, B., Tobias, F., Kijas, A., Tanuji, M., Chen, P., Robinson, P., Taucher-Scholz, G., Suzuki, K., et al (2011). Autophosphorylation and ATM activation: additional sites add to the complexity. Journal of Biological Chemistry, 286(11), 9107-9119. [More Information]
  • Xue, J., Graham, M., Novelle, A., Sue, N., Gray, N., McNiven, M., Smillie, K., Cousin, M., Robinson, P. (2011). Calcineurin Selectively Docks with the Dynamin Ixb Splice Variant to Regulate Activity-dependent Bulk Endocytosis. Journal of Biological Chemistry, 286(35), 30295-30303. [More Information]
  • Chircop (nee Fabbro), M., Sarcevic, B., Larsen, M., Malladi, C., Chau, N., Zavortink, M., Smith, C., Quan, A., Anggono, V., Hains, P., Graham, M., Robinson, P. (2011). Phosphorylation of dynamin II at serine-764 is associated with cytokinesis. BBA: Biochimica et Biophysica Acta - Molecular Cell Research, 1813 (10), 1689-1699. [More Information]

2010

  • Chan, L., Hansra, G., Robinson, P., Graham, M. (2010). Differential Phosphorylation of Dynamin I Isoforms in Subcellular Compartments Demonstrates the Hidden Complexity of Phosphoproteomes. Journal of Proteome Research, 9(8), 4028-4037. [More Information]
  • Baker, M., Smith, N., Hetherington, L., Taubman, K., Graham, M., Robinson, P., Aitken, R. (2010). Label-Free Quantitation of Phosphopeptide Changes During Rat Sperm Capacitation. Journal of Proteome Research, 9(2), 718-729. [More Information]

2009

  • Odell, L., Chau, N., Mariana, A., Graham, M., Robinson, P., McCluskey, A. (2009). Azido and Diazarinyl Analogues of Bis-Tyrphostin as Asymmetrical Inhibitors of Dynamin GTPase. ChemMedChem: chemistry enabling drug discovery, 4(7), 1182-1188.
  • Chircop (nee Fabbro), M., Oakes, V., Graham, M., Ma, M., Smith, C., Robinson, P., Khanna, K. (2009). The actin-binding and bundling protein, EPLIN, is required for cytokinesis. Cell Cycle, 8(5), 757-764. [More Information]

2008

  • Craft, G., Graham, M., Bache, N., Larsen, M., Robinson, P. (2008). The in vivo phosphorylation sites in multiple isoforms of amphiphysin I from rat brain nerve terminals. Molecular and Cellular Proteomics, 7(6), 1146-1161. [More Information]

2007

  • Cohen, S., Graham, M., Lovrecz, G., Bache, N., Robinson, P., Reddel, R. (2007). Protein composition of catalytically active human telomerase from immortal cells. Science, 315(5820), 1850-1853. [More Information]
  • Oganesian, L., Graham, M., Robinson, P., Bryan, T. (2007). Telomerase Recognizes G-Quadruplex and Linear DNA as Distinct Substrates. Biochemistry, 46(40), 11279-11290. [More Information]
  • Graham, M., Kilby, D., Firth, S., Robinson, P., Baxter, R. (2007). The in vivo phosphorylation and glycosylation of human insulin-like growth factor binding protein-5. Molecular and Cellular Proteomics, 6(8), 1392-1405. [More Information]
  • Graham, M., Anggono, V., Bache, N., Larsen, M., Craft, G., Robinson, P. (2007). The in vivo phosphorylation sites of rat brain dynamin I. Journal of Biological Chemistry, 282(20), 14695-14707. [More Information]
  • Acevedo, K., Li, R., Soo, P., Suryadinata, R., Sarcevic, B., Valova, V., Graham, M., Robinson, P., Bernard, O. (2007). The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules. Experimental Cell Research: emphasizing molecular approaches to cell biology, 313(20), 4091-4106. [More Information]

2006

  • Kozlov, S., Graham, M., Peng, C., Chen, P., Robinson, P., Lavin, M. (2006). Involvement of novel autophosphorylation sites in ATM activation. The EMBO Journal, 25(15), 3504-3514.
  • Anggono, V., Smillie, K., Graham, M., Valova, V., Cousin, M., Robinson, P. (2006). Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. Nature Neuroscience, 9(6), 752-760. [More Information]

2005

  • Chircop (nee Fabbro), M., Zhou, B., Takahashi, M., Sarcevic, B., Lal, P., Graham, M., Gabrielli, B., Robinson, P., Nigg, E., Ono, Y., et al (2005). Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Developmental Cell, 9(4), 477-488. [More Information]
  • Hill, T., Odell, L., Edwards, J., Graham, M., McGeachie, A., Rusak, J., Quan, A., Abagyan, R., Scott, J., Robinson, P., et al (2005). Small molecule inhibitors of dynamin I GTPase activity: Development of dimeric tyrphostins. Journal of Medicinal Chemistry, 48(24), 7781-7788.

2004

  • Larsen, M., Graham, M., Robinson, P., Roepstorff, P. (2004). Improved Detection Of Hydrophilic Phosphopeptides Using Graphite Powder Microcolumns And Mass Spectrometry: Evidence For In Vivo Doubly Phosphorylated Dynamin I And Dynamin Iii. Molecular and Cellular Proteomics, 3(5), 456-465.
  • Graham, M., Ruma-Haynes, P., Capes-Davis, A., Dunn, J., Tan, T., Valova, V., Robinson, P., Jeffrey, P. (2004). Multisite Phosphorylation Of Doublecortin By Cyclin-Dependent Kinase 5. Biochemical Journal, 381(2), 471-481.
  • Xue, J., Milburn, P., Hanna, B., Graham, M., Rostas, J., Robinson, P. (2004). Phosphorylation Of Septin 3 On Ser-91 By Cgmp-Dependent Protein Kinase-I In Nerve Terminals. Biochemical Journal, 381(3), 753-760.

2003

  • Tan, T., Valova, V., Malladi, C., Graham, M., Berven, L., Jupp, O., Hansra, G., McClure, S., Sarcevic, B., Boadle, R., Robinson, P., et al (2003). Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), 701-710.

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