Dr Steven Fleming

BSc (Hons); MSc; PhD
Honorary Associate
Anatomy & Histology, School of Medical Sciences

Telephone +61 7 3394 4108
Fax +61 7 3394 4109

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Biographical details

Dr Steven D Fleming PhD MSc BSc (Hons) is a founding Director of Assisted Conception Australia at Greenslopes Private Hospital in Brisbane and an Honorary Associate at the University of Sydney. After completing his PhD in 1987, he undertook postdoctoral research in the Human Reproduction Unit of the Department of Obstetrics and Gynaecology at the Royal North Shore Hospital in Sydney. From 1993-1997 he was appointed Lecturer in Obstetrics and Gynaecology at the University of Nottingham in England, where he established the worlds first Masters degree in Assisted Reproduction Technology with Dr Simon Fishel as well as a Summer School with Prof Lars Hamberger at the University of Gothenburg in Sweden. From 1998-2008 he was the Scientific Director of Westmead Fertility Centre at Westmead Hospital in Sydney and was appointed Senior Lecturer in Obstetrics and Gynaecology at the University of Sydney. He is the recipient of research grants from the National Health and Medical Research Council, the Rebecca L Cooper Medical Research Foundation, the Fertility Society of Australia and the University of Sydney. He is an author and editor of several books, book chapters and numerous articles in peer-reviewed journals.

Research interests

Reproductive biology, including assisted reproduction technology, cryopreservation, endometrial physiology, endometriosis, implantation, micromanipulation and oocyte maturation.

Teaching and supervision

Previous teaching activities include Module Convenor & Member of the Teaching, Assessment & Curriculum Committee in Reproductive Health Sciences & Human Genetics, University of Sydney; Problem based learning Tutor in the Perinatal and Womens Health rotation, University of Sydney; Guest Lecturer in Embryology, University of New South Wales; Guest Senior Faculty Member for workshops in Assisted Reproduction Technology, Chiang Mai University; Guest Lecturer in Human Embryology, Bourn Hall Clinic, Cambridge; Guest Lecturer in Human Embryonic Development, University College London; Principal Lecturer & Senior Tutor in Assisted Reproduction Technology, University of Nottingham; Lecturer in Mammalian Reproduction & Development, University of Leicester; Lecturer in Veterinary Embryology & Reproductive Physiology, University of Bristol.

Selected grants

2001

  • The role of FSH and FF-MAS in the induction of meiotic resumption in the oocyte; Fleming S, Zhongxian L; National Health and Medical Research Council (NHMRC)/Project Grants.
  • The regulation and role of the ubiquitin-like protein: ubiquitin cross-reactive protein in the endometrial decidua of the human uterus during early pregnancy; Fleming S; DVC Research/Research and Development Scheme: Newly Appointed Staff (NAS).

Selected publications

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Books

  • Fleming, S., Cooke, S. (2013). Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia. Australia: VIVID Publishing.
  • Fleming, S., King, R. (2003). Micromanipulation in assisted conception: a user's manual and troubleshooting guide. United Kingdom: Cambridge University Press.

Edited Books

  • Fleming, S., Cooke, S. (2008). Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia. Australia: VIVID Publishing.

Book Chapters

  • Fleming, S. (2009). Cryopreservation of semen and spermatozoa. In Steven Fleming, Simon Cooke (Eds.), Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia, (pp. 229-238). Australia: VIVID Publishing.
  • Griffin, A., Fleming, S. (2008). Female gametogenesis. In Steven Fleming, Simon Cooke (Eds.), Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia. Australia: VIVID Publishing.
  • Fleming, S. (2003). In vitro maturation of oocytes. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 253-266). Chiang Mai, Thailand: Nopburee Press.
  • Fleming, S. (2003). Recent advances in A.R.T: laboratory perspectives. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 279-284). Chiang Mai, Thailand: Nopburee Press.
  • Fleming, S. (2003). Surgical sperm recovery. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 127-138). Chiang Mai, Thailand: Nopburee Press.
  • Fleming, S. (2003). Troubleshooting the IVF laboratory. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 85-88). Chiang Mai, Thailand: Nopburee Press.

Journals

  • Ilad, R., Fleming, S., Murphy, C., Fazleabas, A. (2010). Immunohistochemical study of the ubiquitin-nuclear factor-kB pathway in the endometrium of the baboon (Papio anubis) with and without endometriosis. Reproduction, Fertility and Development, 22(7), 1118-1130. [More Information]
  • Thomson, L., Fleming, S., Barone, K., Zieschang, J., Clark, A. (2010). The effect of repeated freezing and thawing on human sperm DNA fragmentation. Fertility and Sterility, 93(4), 1147-1156. [More Information]
  • Thomson, L., Fleming, S., Aitken, R., De Iuliis, G., Zieschang, J., Clark, A. (2009). Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Human Reproduction, 24(9), 2061-2070. [More Information]
  • Yang, W., Hwu, Y., Lee, R., Li, S., Fleming, S. (2009). Early-cleavage is a reliable predictor for embryo implantation in the GnRH agonist protocols but not in the GnRH antagonist protocols. Reproductive Biology and Endocrinology, 7(20), 20-1-20-7. [More Information]
  • Thomson, L., Fleming, S., Schulke, L., Barone, K., Zieschang, J., Clark, A. (2009). The DNA integrity of cryopreserved spermatozoa separated for use in assisted reproductive technology is unaffected by the type of cryoprotectant used but is related to the DNA integrity of the fresh separated preparation. Fertility and Sterility, 92, 991-1001. [More Information]
  • Fleming, S., Fazleabas, A., Bell, S. (2008). Differential regulation of insulin-like growth factor binding protein-1 and -2 by insulin in the baboon (Papio anubis) endometrium. Reproductive Biology and Endocrinology, 6(Article 6), 6-1-6-11. [More Information]
  • Stoddart, N., Phan, L., Kristiansen, S., Fleming, S. (2008). Differential relationship between the total number of oocytes retrieved and the implantation rate in IVF and ICSI patients. Fertility and Sterility, 90(Supplement), S230.
  • Hua, V., Fleming, S., Illingworth, P. (2008). Effects of protein kinase A and C inhibitors on follicular inhibin and activin during ovulation. Reproductive BioMedicine Online, 17(5), 642-651. [More Information]
  • Fleming, S., Ilad, R., Griffin, A., Ong, K., Wu, Y., Smith, H., Aitken, R. (2008). Membrane-based electrophoresis: an equally efficient method of sperm preparation for IVF and ICSI. Reproductive BioMedicine Online, 16(Supplement 4).
  • Fleming, S., Ilad, R., Griffin, A., Wu, Y., Ong, K., Smith, H., Aitken, R. (2008). Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation. Human Reproduction, 23(12), 2646-2651. [More Information]
  • Yang, W., Hwu, Y., Lee, R., Li, S., Lin, S., Fleming, S. (2007). Early cleavage does not predict treatment outcome following the use of GnRH antagonists in women older than 35. Fertility and Sterility, 88(6), 1573-1578. [More Information]
  • Fleming, S., Ilad, R., Ong, K., Wu, Y., Smith, H., Aitken, J. (2007). First Prospective Controlled Trial of a Novel Membrane-Based Electrophoretic Method of Isolating Spermatozoa for IVF. Australian and New Zealand Journal of Obstetrics and Gynaecology, 47(Supp1), A10-A11.
  • Stoddart, N., Phan, L., Kristiansen, S., Fleming, S. (2007). Inverse correlation between female patient's age and embryonic cavitation rate of human embryos cultured in vitro. Fertility and Sterility, 88(Supp 1), S147-S148.
  • Hwu, Y., Chen, C., Li, S., Chen, P., Lee, R., Fleming, S. (2006). Expression of vascular endothelial growth factor messenger ribonucleic acid and protein in human preimplantation embryos. Fertility and Sterility, 85(6), 1830-1832. [More Information]
  • Griffin, A., Grondahl, C., Fleming, S. (2004). Action Of Hypoxanthine And Meiosis-Activating Sterol On Oocyte Maturation In The Mouse Is Strain Specific. Reproductive BioMedicine Online, 8(6), 673-681.
  • Coticchio, G., Rossi, G., Borini, A., Grondahl, C., Macchiarelli, G., Flamigni, C., Fleming, S., Cecconi, S. (2004). Mouse Oocyte Meiotic Resumption And Polar Body Extrusion In Vitro Are Differentially Influenced By Fsh, Epidermal Growth Factor And Meiosis-Activating Sterol. Human Reproduction, 19(12), 2913-2918.
  • Ilad, R., Fleming, S., Bebington, C., Murphy, C. (2004). Ubiquitin Is Associated With The Survival Of Ectopic Stromal Cells In Endometriosis. Reproductive Biology and Endocrinology, 2(September), 69-76.
  • Stoddart, N., Rho, H., Maisel, L., Zhao, T., Tarantino, S., Fleming, S. (2003). Influence of the position of the culture dish within the incubator chamber and temperature of culture media on clinical outcome following Assisted Reproductive Technology (ART) treatment. Fertility and Sterility, 80(Supp 3), 164-165.
  • Coticchio, G., Cecconi, S., Borini, A., Grondahl, C., Rossi, G., Macchiarelli, G., Griffin, A., Fleming, S. (2003). Meiotic maturation in vitro of mouse oocytes induced by follicle stimulating hormone (FSH), epidermal growth factor (EGF) and follicular fluid meiosis-activating sterol (FF-MAS). Reproduction. Abstract Series, 30, 37-38.
  • Stoddart, N., Rho, H., Hussain, A., Fleming, S., Bernhisel, M. (2002). A comparison of the effects of short-term oocyte culture in glucose/phosphate-free medium or in glucose/phosphate-supplemented medium prior to intracytoplasmic sperm injection (ICSI) on clinical outcome. Fertility and Sterility, 78(3 Supp 1), 80.
  • Roudebush, W., Purnell, E., Stoddart, N., Fleming, S. (2002). Embryonic Platelet-Activating Factor: Temporal Expression of the Ligand and Receptor. Journal Of Assisted Reproduction And Genetics, 19(2), 72-78.

Conferences

  • Thomson, L., Fleming, S., Schulke, L., Barone, K., Zieschang, J., Clark, A. (2008). Cryoprotectants and sperm DNA fragmentation. FSA Annual Meeting.
  • Ilad, R., Fleming, S., Murphy, C., Cooper, M., Fazleabas, A. (2008). IKK-a, a likely kinase for NFkB mediated ectopic endometrial cell survival in the baboon (Papio anubis). 10th World Congress on Endometriosis.
  • Wu, Y., Chapman, S., Harris, J., Martin, S., Ramnac, A., Hua, K., Rodriguez, E., Griffin, A., Baseotto, S., Fleming, S. (2008). The differential effects of Sage and Cook culture media on early embryonic development. FSA Annual Meeting.
  • Deshpande, A., Cohen, R., Tsang, I., Ambler, G., Fleming, S. (2007). Does increase in testicular size after varicocele correction indicate normalisation of testicular function? II World Congress of the World Federation of Associations of Pediatric Surgeons.
  • Fleming, S., Ilad, R., Ong, K., Wu, Y., Smith, H., Aitken, J. (2007). First recorded pregnancy after IVF using electrophoretically isolated spermatozoa. 2nd IVI International Congress.
  • Deshpande, A., Cohen, R., Tsang, I., Ambler, G., Fleming, S. (2007). Is serum FSH a sensitive indicator of predicting long-term outcomes of varicocele correction in adolescence? II World Congress of the World Federation of Associations of Pediatric Surgeons.
  • Ilad, R., Fleming, S., Bebington, C., Murphy, C. (2005). Ubiquitin is associated with the survival of ectopic stromal cells in endometriosis. 9th World Congress on Endometriosis: the patient as a partner.
  • Griffin, A., Fleming, S. (2004). FF-MAS modulates F-actin and connexin 43 localisation during oocyte maturation in the mouse. FSA Annual Meeting.
  • Min, S., Stepanov, D., Fleming, S., Won, Y. (2003). High power, stable figure-eight fiber laser with specially designed FBG both for repetition rate control and for bandwidth enlargement. Conference on Lasers & Electro-Optics International Quantum Electronics Conference (CLEO/IQEC 2004), CD-ROM: Conference on Lasers and Electro-Optics (CLEO).

2013

  • Fleming, S., Cooke, S. (2013). Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia. Australia: VIVID Publishing.

2010

  • Ilad, R., Fleming, S., Murphy, C., Fazleabas, A. (2010). Immunohistochemical study of the ubiquitin-nuclear factor-kB pathway in the endometrium of the baboon (Papio anubis) with and without endometriosis. Reproduction, Fertility and Development, 22(7), 1118-1130. [More Information]
  • Thomson, L., Fleming, S., Barone, K., Zieschang, J., Clark, A. (2010). The effect of repeated freezing and thawing on human sperm DNA fragmentation. Fertility and Sterility, 93(4), 1147-1156. [More Information]

2009

  • Fleming, S. (2009). Cryopreservation of semen and spermatozoa. In Steven Fleming, Simon Cooke (Eds.), Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia, (pp. 229-238). Australia: VIVID Publishing.
  • Thomson, L., Fleming, S., Aitken, R., De Iuliis, G., Zieschang, J., Clark, A. (2009). Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Human Reproduction, 24(9), 2061-2070. [More Information]
  • Yang, W., Hwu, Y., Lee, R., Li, S., Fleming, S. (2009). Early-cleavage is a reliable predictor for embryo implantation in the GnRH agonist protocols but not in the GnRH antagonist protocols. Reproductive Biology and Endocrinology, 7(20), 20-1-20-7. [More Information]
  • Thomson, L., Fleming, S., Schulke, L., Barone, K., Zieschang, J., Clark, A. (2009). The DNA integrity of cryopreserved spermatozoa separated for use in assisted reproductive technology is unaffected by the type of cryoprotectant used but is related to the DNA integrity of the fresh separated preparation. Fertility and Sterility, 92, 991-1001. [More Information]

2008

  • Thomson, L., Fleming, S., Schulke, L., Barone, K., Zieschang, J., Clark, A. (2008). Cryoprotectants and sperm DNA fragmentation. FSA Annual Meeting.
  • Fleming, S., Fazleabas, A., Bell, S. (2008). Differential regulation of insulin-like growth factor binding protein-1 and -2 by insulin in the baboon (Papio anubis) endometrium. Reproductive Biology and Endocrinology, 6(Article 6), 6-1-6-11. [More Information]
  • Stoddart, N., Phan, L., Kristiansen, S., Fleming, S. (2008). Differential relationship between the total number of oocytes retrieved and the implantation rate in IVF and ICSI patients. Fertility and Sterility, 90(Supplement), S230.
  • Hua, V., Fleming, S., Illingworth, P. (2008). Effects of protein kinase A and C inhibitors on follicular inhibin and activin during ovulation. Reproductive BioMedicine Online, 17(5), 642-651. [More Information]
  • Griffin, A., Fleming, S. (2008). Female gametogenesis. In Steven Fleming, Simon Cooke (Eds.), Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia. Australia: VIVID Publishing.
  • Ilad, R., Fleming, S., Murphy, C., Cooper, M., Fazleabas, A. (2008). IKK-a, a likely kinase for NFkB mediated ectopic endometrial cell survival in the baboon (Papio anubis). 10th World Congress on Endometriosis.
  • Fleming, S., Ilad, R., Griffin, A., Ong, K., Wu, Y., Smith, H., Aitken, R. (2008). Membrane-based electrophoresis: an equally efficient method of sperm preparation for IVF and ICSI. Reproductive BioMedicine Online, 16(Supplement 4).
  • Fleming, S., Ilad, R., Griffin, A., Wu, Y., Ong, K., Smith, H., Aitken, R. (2008). Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation. Human Reproduction, 23(12), 2646-2651. [More Information]
  • Fleming, S., Cooke, S. (2008). Textbook of Assisted Reproduction for Scientists in Reproductive Technology: Scientists in Reproductive Technology (SIRT); Fertility Society of Australia. Australia: VIVID Publishing.
  • Wu, Y., Chapman, S., Harris, J., Martin, S., Ramnac, A., Hua, K., Rodriguez, E., Griffin, A., Baseotto, S., Fleming, S. (2008). The differential effects of Sage and Cook culture media on early embryonic development. FSA Annual Meeting.

2007

  • Deshpande, A., Cohen, R., Tsang, I., Ambler, G., Fleming, S. (2007). Does increase in testicular size after varicocele correction indicate normalisation of testicular function? II World Congress of the World Federation of Associations of Pediatric Surgeons.
  • Yang, W., Hwu, Y., Lee, R., Li, S., Lin, S., Fleming, S. (2007). Early cleavage does not predict treatment outcome following the use of GnRH antagonists in women older than 35. Fertility and Sterility, 88(6), 1573-1578. [More Information]
  • Fleming, S., Ilad, R., Ong, K., Wu, Y., Smith, H., Aitken, J. (2007). First Prospective Controlled Trial of a Novel Membrane-Based Electrophoretic Method of Isolating Spermatozoa for IVF. Australian and New Zealand Journal of Obstetrics and Gynaecology, 47(Supp1), A10-A11.
  • Fleming, S., Ilad, R., Ong, K., Wu, Y., Smith, H., Aitken, J. (2007). First recorded pregnancy after IVF using electrophoretically isolated spermatozoa. 2nd IVI International Congress.
  • Stoddart, N., Phan, L., Kristiansen, S., Fleming, S. (2007). Inverse correlation between female patient's age and embryonic cavitation rate of human embryos cultured in vitro. Fertility and Sterility, 88(Supp 1), S147-S148.
  • Deshpande, A., Cohen, R., Tsang, I., Ambler, G., Fleming, S. (2007). Is serum FSH a sensitive indicator of predicting long-term outcomes of varicocele correction in adolescence? II World Congress of the World Federation of Associations of Pediatric Surgeons.

2006

  • Hwu, Y., Chen, C., Li, S., Chen, P., Lee, R., Fleming, S. (2006). Expression of vascular endothelial growth factor messenger ribonucleic acid and protein in human preimplantation embryos. Fertility and Sterility, 85(6), 1830-1832. [More Information]

2005

  • Ilad, R., Fleming, S., Bebington, C., Murphy, C. (2005). Ubiquitin is associated with the survival of ectopic stromal cells in endometriosis. 9th World Congress on Endometriosis: the patient as a partner.

2004

  • Griffin, A., Grondahl, C., Fleming, S. (2004). Action Of Hypoxanthine And Meiosis-Activating Sterol On Oocyte Maturation In The Mouse Is Strain Specific. Reproductive BioMedicine Online, 8(6), 673-681.
  • Griffin, A., Fleming, S. (2004). FF-MAS modulates F-actin and connexin 43 localisation during oocyte maturation in the mouse. FSA Annual Meeting.
  • Coticchio, G., Rossi, G., Borini, A., Grondahl, C., Macchiarelli, G., Flamigni, C., Fleming, S., Cecconi, S. (2004). Mouse Oocyte Meiotic Resumption And Polar Body Extrusion In Vitro Are Differentially Influenced By Fsh, Epidermal Growth Factor And Meiosis-Activating Sterol. Human Reproduction, 19(12), 2913-2918.
  • Ilad, R., Fleming, S., Bebington, C., Murphy, C. (2004). Ubiquitin Is Associated With The Survival Of Ectopic Stromal Cells In Endometriosis. Reproductive Biology and Endocrinology, 2(September), 69-76.

2003

  • Min, S., Stepanov, D., Fleming, S., Won, Y. (2003). High power, stable figure-eight fiber laser with specially designed FBG both for repetition rate control and for bandwidth enlargement. Conference on Lasers & Electro-Optics International Quantum Electronics Conference (CLEO/IQEC 2004), CD-ROM: Conference on Lasers and Electro-Optics (CLEO).
  • Fleming, S. (2003). In vitro maturation of oocytes. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 253-266). Chiang Mai, Thailand: Nopburee Press.
  • Stoddart, N., Rho, H., Maisel, L., Zhao, T., Tarantino, S., Fleming, S. (2003). Influence of the position of the culture dish within the incubator chamber and temperature of culture media on clinical outcome following Assisted Reproductive Technology (ART) treatment. Fertility and Sterility, 80(Supp 3), 164-165.
  • Coticchio, G., Cecconi, S., Borini, A., Grondahl, C., Rossi, G., Macchiarelli, G., Griffin, A., Fleming, S. (2003). Meiotic maturation in vitro of mouse oocytes induced by follicle stimulating hormone (FSH), epidermal growth factor (EGF) and follicular fluid meiosis-activating sterol (FF-MAS). Reproduction. Abstract Series, 30, 37-38.
  • Fleming, S., King, R. (2003). Micromanipulation in assisted conception: a user's manual and troubleshooting guide. United Kingdom: Cambridge University Press.
  • Fleming, S. (2003). Recent advances in A.R.T: laboratory perspectives. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 279-284). Chiang Mai, Thailand: Nopburee Press.
  • Fleming, S. (2003). Surgical sperm recovery. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 127-138). Chiang Mai, Thailand: Nopburee Press.
  • Fleming, S. (2003). Troubleshooting the IVF laboratory. In T Vutyavanich (Eds.), Assisted Reproductive Technology, (pp. 85-88). Chiang Mai, Thailand: Nopburee Press.

2002

  • Stoddart, N., Rho, H., Hussain, A., Fleming, S., Bernhisel, M. (2002). A comparison of the effects of short-term oocyte culture in glucose/phosphate-free medium or in glucose/phosphate-supplemented medium prior to intracytoplasmic sperm injection (ICSI) on clinical outcome. Fertility and Sterility, 78(3 Supp 1), 80.
  • Roudebush, W., Purnell, E., Stoddart, N., Fleming, S. (2002). Embryonic Platelet-Activating Factor: Temporal Expression of the Ligand and Receptor. Journal Of Assisted Reproduction And Genetics, 19(2), 72-78.

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