Cardiac Proteomics Laboratory

Lab head: Brett Hambly
Location: Biochemistry Building

Research interests include the pathophysiology of ischaemic myocardial injury and inherited cardiovascular disease, particularly diseases of the aorta and hypertrophic cardiomyopathy. Clinical research studies have quantified predictors of infarct size for individual patients and developed a model for early risk stratification of individual patients with acute infarcts. Research conducted in this laboratory has evolved from a physiological platform to a molecular approach through proteomics. Recent work has achieved international recognition through publications, presentations at American Heart Association and American College of Cardiology meetings and invitation of a PhD student for a post-doctoral position at Johns Hopkins Hospital.

Lab members: S Cordwell (head), R Jeremy (head), B Hambly (head)

The role of calcium overload in myocardial I/R injury

Primary supervisor: Stuart Cordwell

At the cardiomyocyte level, I/R injury is characterized by Ca2+ overload and the generation of ROS. Both are highly reactive molecules and are able to interact with almost any biological substrate. Lipids and proteins are possible targets, with potentially wide-reaching implications - lipid damage will affect the integrity and permeability of cell membranes, while protein damage may change the functionality of regulatory enzymes or contractile mediators. We have previously investigated the role of ROS on protein post-translational modification in I/R injury. In this project, proteomics will be used to investigate the effects of Ca2+ overload on proteins within myocardial tissue during I/R. The project will involve animal studies (Langendorf perfusion surgery), as well as biochemical assays including two-dimensional electrophoresis and mass spectrometry. These studies will identify proteins damaged during I/R and potentially lead to clinical therapies.

Discipline: Pathology
Co-supervisors: Brett Hambly