Cell Therapy in Transplantation

Lab head: Min Hu
Location: The Westmead Institute for Medical Research, Westmead Clinical School

The main research interest is the development of therapeutic strategies to improve the clinical outcomes of islet and kidney transplantation to achieve complete immunological tolerance to allografts. These include investigating the immune and genetics profile of islet and kidney transplant patients, generating allospecific Tregs, developing novel regimens for tolerance induction in humanised mouse models of islet transplantation, and developing clinical grade Tregs for clinical trials.

Lab members: Yi wen Qian
Funding: GL Class Code: 2115
Research approach equipment: 1) Flow cytometric analysis 2) Cell sorting 3) Real time RT-PCR 4)Immunohistochemistry (IHC) and immunofluorescence staining. 5) Mouse models of islet, kidney, skin and heart transplants.

1. Hu, M., B. Kramer, G. Y. Zhang, Y. M. Wang, D. Watson, B. Howden, G. McCowage, I. E. Alexander, P. Gunning, and S. I. Alexander. (2015). Methyl-Guanine-Methyl-Transferase Transgenic Bone Marrow Transplantation Allows N,N-bis(2-chloroethyl)-Nitrosourea Driven Donor Mixed-Chimerism Without Graft-Versus-Host Disease, and With Donor-Specific Allograft Tolerance. Transplantation. 99(12):2476-84.

2.Jin, X., Y. Wang, W. J. Hawthorne, M. Hu, S. Yi, and P. O'Connell.(2014). Enhanced suppression of the xenogeneic T-cell response in vitro by xenoantigen stimulated and expanded regulatory T cells. Transplantation97: 30-38.

3. HHu, M., C. Wang, G. Y. Zhang, M. Saito, Y. M. Wang, M. A. Fernandez, Y. Wang, H. Wu, W. J. Hawthorne, C. Jones, P. J. O'Connell, T. Sparwasser, G. A. Bishop, A. F. Sharland, and S. I. Alexander. (2013). Infiltrating Foxp3(+) regulatory T cells from spontaneously tolerant kidney allografts demonstrate donor-specific tolerance. Am J Transplant13: 2819-2830.u, M., J. Wu, G. Y. Zhang, Y. M. Wang, D. Watson, S. Yi, W. J. Hawthorne, P. J. O'Connell, and S. I. Alexander. (2013).

4. Selective depletion of alloreactive T cells leads to long-term islet allograft survival across a major histocompatibility complex mismatch in diabetic mice. Cell Transplant 22: 1929-1941.

5. Alexander, S. I., N. Smith, M. Hu, D. Verran, A. Shun, S. Dorney, A. Smith, B. Webster, P. J. Shaw, A. Lammi, and M. O. Stormon. (2008). Chimerism and tolerance in a recipient of a deceased-donor liver transplant.N Engl J Med 358: 369-374.

Induction Of Transplant Tolerance in Mouse Transplantation Models

Primary supervisor: Min Hu

IL-7 plays an important role on memory regulatory T cells (Tregs) for their maintenance in peripheral tissues. IL-7/anti-IL-7mAb complexes restore T cell development and induce homeostatic T cell expansion without lymphopenia in IL-7-deficient mice.  The study aims to investigate the role of IL-7 on supporting CD4+Foxp3+ Treg survival in the alloresponse and whether IL-7/anti-IL-7mAb complexes can induce Treg  expansion in a murine skin and islet transplant models.

Discipline: Applied Medical Sciences, Westmead
Co-supervisors: Stephen Alexander, Philip O'Connell, Wayne Hawthorne
Keywords: Translational research, Transplantation, Immune response