Haematology Research

Lab head: Freda Janet Passam
Location: Heart Reserach Institute Labs, Level 3 East, Charles Perkins Centre

Developing biochips for the evaluation of haemostasis and thrombosis

Primary supervisor: Freda Passam

Aim: To develop microfluidic devices which can detect the thrombotic or bleeding tendency in patients with clotting problems.

Background: Many patients with bleeding and clotting disorders go undetected by routine laboratory tests in part because the available assays do not reflect the conditions in the circulation. The Haematology Research Group uses biochips in a microfluidic system that allows blood to flow through passages under controlled conditions. The passages are designed to mimic blood vessels and include features e.g. stenosis, that simulate the circulation in stenosed vessels. The flow of blood through these biochips generates thrombi that can be visualized by real-time microscopy and quantified.

Project overview: This project will study blood cell adhesion and thrombus formation in the microfluidic devices to assess for persisting thrombotic tendency in patients with a history of venous clots, who have completed treatment. Samples from patients with bleeding disorders, on treatment, will be assessed for haemostatic potential. Blood cells and proteins which participate in clot formation, will be measured in the microfluidics system including platelets, fibrin, neutrophil extracellular traps and von Willebrand factor.

Techniques: This project involves the preparation of the microfluidics chips, perfusion assay and microscopy.


Relevant publications:

1. Herbig BA, Yu X, Diamond S. Using microfluidic devices to study thrombosis in pathological blood flows. Biomicrofluidics; 2018;12(4)

2. Lee KH, Cavanaugh L, Leung H, Yan F, Ahmadi Z, Chong BH, Passam F. Quantification of NETs-associated markers by flow cytometry and serum assays in patients with thrombosis and sepsis. Int J Lab Hematol; 2018;40(4):392-399.

3. Zhang C, Neelamegham S. Application of microfluidic devices in studies of thrombosis and hemostasis. Platelets. 2017;28(5):434-440.

Discipline: Pathology
Keywords: Thrombosis, Diagnostic tests, Coagulation and platelets