Multiplex STI testing

The project commenced in March of 2006. The main aim of the project was to develop and validate a Multiplex Polymerase Chain Reaction - Reverse Line Blot (mPCR-RLB)assay, which allows the rapid detection of multiple organisms in very low concentrations.

We developed a method using this technology to detect the presence of the following 14 organisms in urine, endocervical and self-collected vaginal swabs.

  • Chlamydia trachomatis
  • Neisseria gonorrhoeae
  • Mycoplasma genitalium
  • Ureaplasma urealyticum
  • Ureaplasma parvum
  • Trichomonas vaginalis
  • Mycoplasma hominis
  • Gardnerella vaginalis
  • Herpes Simplex Virus type 1 and 2
  • adenovirus
  • Neisseria meningitidis
  • Haemophilus influenzae, and
  • Streptococcus pneumoniae.

Urine specimens from males with and without urethritis and urine, endocervial and self-collected swabs from females with and without vaginal symptoms attending sexual health clinics were collected and analysed using the mPCR-RLB method. The detection of the organisms was then linked to demographic, behavioural and clinical data.

Miss Michelle McKechnie

Michelle completed a Medical Science Degree at the University of Western Sydney. She then went on to complete her Honours degree in Forensic Science also at the University of Western Sydney. She has currently completed her PhD at the University of Sydney, based at STIRC.

Manuscript

For further information Michelle can be contacted at: