Dr Ingrid Gelissen

MAgSci PhD
Senior Lecturer, Pharmaceutics

A15 - Pharmacy And Bank Building
The University of Sydney

Telephone +61 2 86270357

Biographical details

Dr Gelissen initially completed a Masters in Nutrition at Wageningen University, the Netherlands. After working as a research associate for several years overseas and in Sydney, she completed her PhD degree at the Heart Research Institute, the University of Sydney, supported by an NH&MRC scholarship. Since graduating in 2000, she has completed post-doctoral positions at UT Southwestern in Dallas, Texas and the Centre for Vascular Research at the University of New South Wales in Sydney where she was awarded a prestigious Vice-Chancellors Post-doctoral Fellowship. She joined the Faculty of Pharmacy as a lecturer in 2010 and was promoted to senior lecturer in 2014. In 2017, she was awarded a University of Sydney Thompson Fellowship to foster her research into the role of ABC transporter proteins in the setting of Alzheimers disease.

Research interests

• The role of ABC-lipid transporters in health and disease, including cardiovascular disease and inflammation in the lung (in collaboration with Prof. Alaina Ammit, UTS and Woolcock Institute, Sydney).

ABC-transporters are membrane proteins involved in the translocation of substrates across cellular membranes. A number of lipid transporters, including ABCA1 and ABCG1, are involved in removing cholesterol and other lipids from cells, a process that is important in the maintenance of cellular lipid homeostasis. Disregulation of cholesterol homeostasis is of interest to a number of diseases including atherosclerosis, which causes cardiovascular disease and neurogenerative diseases. The basic mechanisms by which these transporters operate is poorly understood, as is their post-translational regulation. These aspects are under investigation in the Gelissen lab, including the role of E3-ubiquitin ligases in the modulation of transporter function.

• New insights into the contribution of ABC-transporters to the export of amyloid-beta peptides from cells in the brain (in collaboration with A/Prof. Richard Callaghan, ANU Canberra).

Alzheimer’s disease (AD) is the most common form of dementia and is a large growing burden on our health system. A critical factor is the accumulation of amyloid-beta (Ab) peptides in the brain, which are toxic to neurons and impair synaptic function. The mechanism of export of Ab peptides from neurons and removal via efflux across the blood brain barrier (BBB) endothelium has been poorly elucidated. Several members of the ABC transporter family have been implicated in this process, including ABCB1 (better known as P-glycoprotein) and lipid transporters such as ABCG1 and ABCG4. This project investigates common mechanisms by which these transporters are regulated and why this specific ABC-transporter mediated clearance pathway may be compromised in AD.

Teaching and supervision

Teaching:

  • Pharmacokinetics/pharmacogenomics
  • Cardiovascular medicines

Current PhD students:

1) Characterisation of the cell biology of the ATP-binding cassette transporter G4 (ABCG4) lipid transporter – Alryel Yang

2) Characterisation of interacting partners of ABC lipid transporters – Amjad Alrosan

Keywords

Alzheimer's disease; ABC transporters; Cardiovascular diseases; Cell biology; Lipid metabolism

Selected grants

2017

  • Ultra-high-performance microbalance, XPR2; Varamini P, Duong H, Grewal T, Kayser V, Gelissen I, Chrzanowski W, Boddy A; University of Sydney, Faculty of Pharmacy/Equipment Grant.
  • Interaction of ABC transporters and amyloid-beta peptides in brain cells; Gelissen I; DVC Research/Thompson Fellowships.

2011

  • The use of Statins in Children - an audit of prescribing data from Australian Children's Hospitals; Moles R, Gelissen I, Aslani P; Society of Hospital Pharmacists of Australia/Research and Development Grants Program.
  • Control of expression and activity of the ABCG1 cholesterol transporter in vascular cells; Gelissen I, Brown A, Brown A; National Health and Medical Research Council (NHMRC)/Project Grants.

Selected publications

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Book Chapters

  • Yang, A., Gelissen, I. (2017). ABC-transporter mediated sterol export from cells using radiolabeled sterols. In Ingrid C. Gelissen and Andrew J. Brown (Eds.), Cholesterol Homeostasis: Methods and Protocols, (pp. 275-285). New York: Springer. [More Information]
  • Gelissen, I., Brown, A. (2017). An overview of cholesterol homeostasis. In Ingrid C. Gelissen and Andrew J. Brown (Eds.), Cholesterol Homeostasis: Methods and Protocols, (pp. 1-6). New York: Springer. [More Information]
  • Gelissen, I., Brown, A., Luu, W. (2017). Manipulating cholesterol status within cells. In Thomas Lion (Eds.), Human Fungal Pathogen Identification : Methods and Protocols, (pp. 41-52). New York: Humana Press. [More Information]

Journals

  • Chai, A., Ammit, A., Gelissen, I. (2017). Examining the role of ABC lipid transporters in pulmonary lipid homeostasis and inflammation. Respiratory Research, 18(1), 1-9. [More Information]
  • Pandzic, E., Gelissen, I., Whan, R., Barter, P., Sviridov, D., Gaus, K., Rye, K., Cochran, B. (2017). The ATP binding cassette transporter, ABCG1, localizes to cortical actin filaments. Scientific Reports, 7, 1-10. [More Information]
  • Rumzhum, N., Patel, B., Prabhala, P., Gelissen, I., Oliver, B., Ammit, A. (2016). IL-17A increases TNF-(alpha)-induced COX-2 protein stability and augments PGE2 secretion from airway smooth muscle cells: impact on (beta)2 -adrenergic receptor desensitization. Allergy, 71(3), 387-396. [More Information]
  • Luu, W., Sharpe, L., Capell-Hattam, I., Gelissen, I., Brown, A. (2016). Oxysterols: Old Tale, New Twists. Annual Review of Pharmacology and Toxicology, 56, 447-467. [More Information]
  • Rahman, M., Prunte, L., Lebender, L., Patel, B., Gelissen, I., Hansbro, P., Morris, J., Clark, A., Verrills, N., Ammit, A. (2016). The phosphorylated form of FTY720 activates PP2A, represses inflammation and is devoid of S1P agonism in A549 lung epithelial cells. Scientific Reports, 6, 1-8. [More Information]
  • Sharpe, L., Rao, G., Jones, P., Glancey, E., Aleidi, S., George, A., Brown, A., Gelissen, I. (2015). Cholesterol sensing by the ABCG1 lipid transporter: Requirement of a CRAC motif in the final transmembrane domain. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 1851 (7), 956-964. [More Information]
  • Aleidi, S., Howe, V., Sharpe, L., Yang, A., Rao, G., Brown, A., Gelissen, I. (2015). The E3 Ubiquitin Ligases, HUWE1 and NEDD4-1, Are Involved in the Post-translational Regulation of the ABCG1 and ABCG4 Lipid Transporters. The Journal of Biological Chemistry, 290(40), 24604-24613. [More Information]
  • Olivier, M., Bott, R., Frisdal, E., Nowicki, M., Plengpanich, W., Desmarchelier, C., Roi, S., Quinn, C., Gelissen, I., Jessup, W., et al (2014). ABCG1 is involved in vitamin e efflux. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 1841 (12), 1741-1751. [More Information]
  • Hsieh, V., Kim, M., Gelissen, I., Brown, A., Sandoval, C., Hallab, J., Kockx, M., Traini, M., Jessup, W., Kritharides, L. (2014). Cellular Cholesterol Regulates Ubiquitination and Degradation of the Cholesterol Export Proteins ABCA1 and ABCG1. The Journal of Biological Chemistry, 289(11), 7524-7536. [More Information]
  • Gelissen, I., Nguyen, H., Tiao, D., Ayoub, R., Aslani, P., Moles, R. (2014). Statin Use in Australian Children: A Retrospective Audit of Four Pediatric Hospitals. Pediatric Drugs, 16(5), 417-423. [More Information]
  • Gelissen, I., McLachlan, A. (2014). The Pharmacogenomics of Statins. Pharmacological Research, 88, 99-106. [More Information]
  • Burns, V., Sharpe, L., Gelissen, I., Brown, A. (2013). Species variation in ABCG1 isoform expression: Implications for the use of animal models in elucidating ABCG1 function. Atherosclerosis, 226(2), 408-411. [More Information]
  • Luu, W., Sharpe, L., Gelissen, I., Brown, A. (2013). The Role of Signalling in Cellular Cholesterol Homeostasis. IUBMB Life, 65(8), 675-684. [More Information]
  • Gelissen, I., Brown, A. (2012). Predicting response to statins by pharmacogenetic testing. Pharmacogenomics, 13(11), 1223-1225. [More Information]
  • Gelissen, I., Sharpe, L., Sandoval, C., Rao, G., Kockx, M., Kritharides, L., Jessup, W., Brown, A. (2012). Protein kinase A modulates the activity of a major human isoform of ABCG1. Journal of Lipid Research, 53(10), 2133-2140. [More Information]
  • Gelissen, I., Brown, A. (2011). Drug targets beyond HMG-CoA reductase: Why venture beyond the statins? Frontiers in Biology, 6(3), 197-205. [More Information]
  • Kerr, I., Haider, A., Gelissen, I. (2011). The ABCG family of membrane-associated transporters: you don't have to be big to be mighty. British Journal of Pharmacology, 164(7), 1767-1779. [More Information]
  • Calabresi, L., Favari, E., Moleri, E., Adorni, M., Pedrelli, M., Costa, S., Jessup, W., Gelissen, I., Kovanen, P., Bernini, F., et al (2009). Functional LCAT is not required for macrophage cholesterol efflux to human serum. Atherosclerosis, 204, 141-146. [More Information]
  • Out, R., Jessup, W., Le Goff, W., Hoekstra, M., Gelissen, I., Zhao, Y., Kritharides, L., Chimini, G., Kuiper, J., Chapman, M., et al (2008). Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporter A1 and G1. Circulation Research, 102, 113-120. [More Information]
  • Wong, J., Quinn, C., Gelissen, I., Jessup, W., Brown, A. (2008). The effect of statins on ABCA1 and ABCG1 expression in human macrophages is influenced by cellular cholesterol levels and extent of differentiation. Atherosclerosis, 196(1), 180-189. [More Information]
  • Wong, J., Quinn, C., Gelissen, I., Brown, A. (2007). Endogenous 24(S),25-Epoxycholesterol Fine-tunes Acute Control of Cellular Cholesterol Homeostasis. Journal of Biological Chemistry, 283, 700-707.
  • Kim, W., Rahmanto, A., Kamili, A., Rye, K., Guillemin, G., Gelissen, I., Jessup, W., Hill, A., Garner, B. (2007). Role of ABCG1 and ABCA1 in Regulation of Neuronal Cholesterol Efflux to Apolipoprotein E Discs and Suppression of Amyloid- Peptide Generation. Journal of Biological Chemistry, 282, 2851-2861.
  • Glaros, E., Kim, W., Quinn, C., Wong, J., Gelissen, I., Jessup, W., Garner, B. (2005). Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-I pathway: 1-phenyl-2-decanoylamino-3-morpholino-1-propanol is a novel cholesterol efflux accelerator. Journal of Biological Chemistry, 280, 24515-24523.

Conferences

  • Aslani, P., Tiao, D., Gelissen, I., Moles, R. (2011). A retrospective study on statin use in children and adolescents. Australasian Pharmaceutical Science Association (2011), Unkown: University Of South Australia.
  • Tiao, D., Aslani, P., Gelissen, I., Moles, R. (2011). The use of statins in children with cholesterol disorders. Medicines Management 2011, the 37th Society of Hospital Pharmacists of Australia (SHPA) National Conference.

2017

  • Yang, A., Gelissen, I. (2017). ABC-transporter mediated sterol export from cells using radiolabeled sterols. In Ingrid C. Gelissen and Andrew J. Brown (Eds.), Cholesterol Homeostasis: Methods and Protocols, (pp. 275-285). New York: Springer. [More Information]
  • Gelissen, I., Brown, A. (2017). An overview of cholesterol homeostasis. In Ingrid C. Gelissen and Andrew J. Brown (Eds.), Cholesterol Homeostasis: Methods and Protocols, (pp. 1-6). New York: Springer. [More Information]
  • Chai, A., Ammit, A., Gelissen, I. (2017). Examining the role of ABC lipid transporters in pulmonary lipid homeostasis and inflammation. Respiratory Research, 18(1), 1-9. [More Information]
  • Gelissen, I., Brown, A., Luu, W. (2017). Manipulating cholesterol status within cells. In Thomas Lion (Eds.), Human Fungal Pathogen Identification : Methods and Protocols, (pp. 41-52). New York: Humana Press. [More Information]
  • Pandzic, E., Gelissen, I., Whan, R., Barter, P., Sviridov, D., Gaus, K., Rye, K., Cochran, B. (2017). The ATP binding cassette transporter, ABCG1, localizes to cortical actin filaments. Scientific Reports, 7, 1-10. [More Information]

2016

  • Rumzhum, N., Patel, B., Prabhala, P., Gelissen, I., Oliver, B., Ammit, A. (2016). IL-17A increases TNF-(alpha)-induced COX-2 protein stability and augments PGE2 secretion from airway smooth muscle cells: impact on (beta)2 -adrenergic receptor desensitization. Allergy, 71(3), 387-396. [More Information]
  • Luu, W., Sharpe, L., Capell-Hattam, I., Gelissen, I., Brown, A. (2016). Oxysterols: Old Tale, New Twists. Annual Review of Pharmacology and Toxicology, 56, 447-467. [More Information]
  • Rahman, M., Prunte, L., Lebender, L., Patel, B., Gelissen, I., Hansbro, P., Morris, J., Clark, A., Verrills, N., Ammit, A. (2016). The phosphorylated form of FTY720 activates PP2A, represses inflammation and is devoid of S1P agonism in A549 lung epithelial cells. Scientific Reports, 6, 1-8. [More Information]

2015

  • Sharpe, L., Rao, G., Jones, P., Glancey, E., Aleidi, S., George, A., Brown, A., Gelissen, I. (2015). Cholesterol sensing by the ABCG1 lipid transporter: Requirement of a CRAC motif in the final transmembrane domain. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 1851 (7), 956-964. [More Information]
  • Aleidi, S., Howe, V., Sharpe, L., Yang, A., Rao, G., Brown, A., Gelissen, I. (2015). The E3 Ubiquitin Ligases, HUWE1 and NEDD4-1, Are Involved in the Post-translational Regulation of the ABCG1 and ABCG4 Lipid Transporters. The Journal of Biological Chemistry, 290(40), 24604-24613. [More Information]

2014

  • Olivier, M., Bott, R., Frisdal, E., Nowicki, M., Plengpanich, W., Desmarchelier, C., Roi, S., Quinn, C., Gelissen, I., Jessup, W., et al (2014). ABCG1 is involved in vitamin e efflux. Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids, 1841 (12), 1741-1751. [More Information]
  • Hsieh, V., Kim, M., Gelissen, I., Brown, A., Sandoval, C., Hallab, J., Kockx, M., Traini, M., Jessup, W., Kritharides, L. (2014). Cellular Cholesterol Regulates Ubiquitination and Degradation of the Cholesterol Export Proteins ABCA1 and ABCG1. The Journal of Biological Chemistry, 289(11), 7524-7536. [More Information]
  • Gelissen, I., Nguyen, H., Tiao, D., Ayoub, R., Aslani, P., Moles, R. (2014). Statin Use in Australian Children: A Retrospective Audit of Four Pediatric Hospitals. Pediatric Drugs, 16(5), 417-423. [More Information]
  • Gelissen, I., McLachlan, A. (2014). The Pharmacogenomics of Statins. Pharmacological Research, 88, 99-106. [More Information]

2013

  • Burns, V., Sharpe, L., Gelissen, I., Brown, A. (2013). Species variation in ABCG1 isoform expression: Implications for the use of animal models in elucidating ABCG1 function. Atherosclerosis, 226(2), 408-411. [More Information]
  • Luu, W., Sharpe, L., Gelissen, I., Brown, A. (2013). The Role of Signalling in Cellular Cholesterol Homeostasis. IUBMB Life, 65(8), 675-684. [More Information]

2012

  • Gelissen, I., Brown, A. (2012). Predicting response to statins by pharmacogenetic testing. Pharmacogenomics, 13(11), 1223-1225. [More Information]
  • Gelissen, I., Sharpe, L., Sandoval, C., Rao, G., Kockx, M., Kritharides, L., Jessup, W., Brown, A. (2012). Protein kinase A modulates the activity of a major human isoform of ABCG1. Journal of Lipid Research, 53(10), 2133-2140. [More Information]

2011

  • Aslani, P., Tiao, D., Gelissen, I., Moles, R. (2011). A retrospective study on statin use in children and adolescents. Australasian Pharmaceutical Science Association (2011), Unkown: University Of South Australia.
  • Gelissen, I., Brown, A. (2011). Drug targets beyond HMG-CoA reductase: Why venture beyond the statins? Frontiers in Biology, 6(3), 197-205. [More Information]
  • Kerr, I., Haider, A., Gelissen, I. (2011). The ABCG family of membrane-associated transporters: you don't have to be big to be mighty. British Journal of Pharmacology, 164(7), 1767-1779. [More Information]
  • Tiao, D., Aslani, P., Gelissen, I., Moles, R. (2011). The use of statins in children with cholesterol disorders. Medicines Management 2011, the 37th Society of Hospital Pharmacists of Australia (SHPA) National Conference.

2009

  • Calabresi, L., Favari, E., Moleri, E., Adorni, M., Pedrelli, M., Costa, S., Jessup, W., Gelissen, I., Kovanen, P., Bernini, F., et al (2009). Functional LCAT is not required for macrophage cholesterol efflux to human serum. Atherosclerosis, 204, 141-146. [More Information]

2008

  • Out, R., Jessup, W., Le Goff, W., Hoekstra, M., Gelissen, I., Zhao, Y., Kritharides, L., Chimini, G., Kuiper, J., Chapman, M., et al (2008). Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporter A1 and G1. Circulation Research, 102, 113-120. [More Information]
  • Wong, J., Quinn, C., Gelissen, I., Jessup, W., Brown, A. (2008). The effect of statins on ABCA1 and ABCG1 expression in human macrophages is influenced by cellular cholesterol levels and extent of differentiation. Atherosclerosis, 196(1), 180-189. [More Information]

2007

  • Wong, J., Quinn, C., Gelissen, I., Brown, A. (2007). Endogenous 24(S),25-Epoxycholesterol Fine-tunes Acute Control of Cellular Cholesterol Homeostasis. Journal of Biological Chemistry, 283, 700-707.
  • Kim, W., Rahmanto, A., Kamili, A., Rye, K., Guillemin, G., Gelissen, I., Jessup, W., Hill, A., Garner, B. (2007). Role of ABCG1 and ABCA1 in Regulation of Neuronal Cholesterol Efflux to Apolipoprotein E Discs and Suppression of Amyloid- Peptide Generation. Journal of Biological Chemistry, 282, 2851-2861.

2005

  • Glaros, E., Kim, W., Quinn, C., Wong, J., Gelissen, I., Jessup, W., Garner, B. (2005). Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-I pathway: 1-phenyl-2-decanoylamino-3-morpholino-1-propanol is a novel cholesterol efflux accelerator. Journal of Biological Chemistry, 280, 24515-24523.

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