Research Facilities in the Faculty of Pharmacy

Our research programs are supported by a range of cutting edge facilities and technologies, driving investigations into many key areas of Pharmaceutical Sciences and Pharmacy Practice.

Our facilities include:

Molecular Biology

Tissue Culture Facility

  • Mammalian cell culture, room N257b (PC1), N423 (PC2) and S208 (PC2)
  • Animal procedures, room S226
  • Bacteria and yeast culture, room N267, N412 and S238a

Electrophysiology (QC1 facility)

Oocyte Culture, room N261 (QC1) and S201 (QC1)

  • Olympus inverted microscope
  • Olympus polarising microscope (Sz-61 and fibre optic light)
  • Single channel oocyte recording system
  • Multi-channel roboocyte system
  • Gene pulsar excel electroporation system

Nucleic Acid and Protein Analysis

RNA, DNA and Protein Quantification

  • Bioanalyser Agilent 2100, room S222
  • NanoDrop ND-1000 Spectrophotometer, room N261 (QC1)

PCR and Real Time PCR

  • Rotor-Gene Q 2plex system real time PCR, room S222
  • Bio-Rad Sequence detection system 7500, room S222
  • Applied Biosystems 2720 Themal Cycler, room N266

Gel Electrophoresis

  • Protein Gel and XCell Surelock mini-cell electrophoresis system, room N267, S238a and N412
  • DNA agarose electrophoresis, room N266 and N412

Gel Imaging

  • Gel Doc EZ Image, room S222
  • ImageQuant (TM) LAS 500, room N266
  • Konica SRX101 Medical Film Processor, room S237

Microplate Reader

  • Bio-Rad 680 microplate reader, room S222
  • POLARstar Optima/FLUORstar, room S222
  • Tecan GENios F129004 microplate reader and plate washer, room N431

Flowcytometer

  • BD FACSCalibu (TM), room N412

Centrifuge

  • Thermo Scientific Sorvall RC6 Plus Superspeed Centrifuge, room N257E
  • MiVac Quattro super vacuum, room N257E
  • Hettich Mickro 220R refrigerated centrifuge, room N257E

Protein Purification and Interaction

Protein Purification

  • GE Healthcare ÄKTApurifie (TM) UPC10 Core system, room S238a
  • GE Healthcare ÄKTAFPLC, room S206

Scintillation Analyser

  • Hidex 300 SL Liquid Scintillation Counter, room N257D

Nano Biocharacterisation

Nano-IR

The nanoIR is a level-based measurement tool that reveals the chemical composition of samples at the nanoscale. This system combines key elements of both nanoscale IR spectroscopy and atomic force microscopy (AFM) to enable the acquisition of infrared spectra at spatial resolutions well beyond the optical diffraction limit.


In addition to revealing chemical composition, the nanoIR system provides high-resolution characterization of local topographic, mechanical, and thermal properties. Potential application areas span the realms of polymer science, materials science, and life science, including detailed studies of structure-property correlations. Room N236.


Microscopy

Inverted Microscope

  • With fluorescence DMI400B, room S238b
  • Ti-U, room S200
  • Ti-U MESS3220, room S238b

Atomic Force Microscope

  • With molecular force probe, MFP-3D-Bio, room S238b
  • Veeco, multimode, room S232
  • Park XE70 with SThM-scanning thermo XE-70, room S232

Scanning Ion Occlusion Microscope

  • Ionscope, room S200
  • With sensing system qNano, IZON, room S237a

Other microscopes

  • P-97 micropipette puller, room S210
    Halcyonics active vibration isolation, room S200

Pharmaceutical and Medicinal Chemistry

Chromatography

High Performance Liquid Chromatography-HPLC

  • Nexera UHPLC (Photodiode array detector), room N355
  • HPLC Prominence (Photodiode array detector and fraction collector), room N355
  • HPLC Preparative (UV and refractive index detectors), room N214
  • HPLC Prominence (UV and refractive index detectors), room S211
  • HPLC Agilent 1200 (UV detector), room S211

Gas Chromatography-GC

  • GC-2010AF Plus with SPL/FID, room N355

Flash Chromatography

  • Reveleris┬« X2 with ELSD detector, room N214

Thin Layer Chromatography-TLC

  • HPTLC, room N412
  • UV Viewer, room N355

Solid and Liquid Phase Extraction

  • SPE-ASPEC-XL4, room S245

Chemical and Peptide Synthesis

Microwave Reactor

  • CEM Discover S-class, room S236

Peptide Synthesis

  • Automated peptide synthesizer, room N431

Evaporators/dryers

  • Rotary-evaporators, rooms S236, N214 and N431
  • Gas-drying unit, room N214
  • Freeze dryer, room N257E
  • Centrifugal Evaporator, room N257E

Pharmaceutical Formulation

Physical/Chemical Characterisation

Particle sizing, room 211

  • Nanosizer: capable of measuring Brownian motion and Zeta potential for practical size between 0.1 nm-1000 nm
  • Mastersizer 2000: capable of measuring wet and dry sample by laser diffraction
  • Spraytec: capable of measuring size of aerosol by laser diffraction
  • Impactors to characterise aerodynamic size of aerosols: Anderson sampler, Multistage liquid Impinger, Marple Millers Cascade Impactors, and Next Generation Impactors

Viscosity measurement, room 211

  • DVIII Rheometer: to measure viscosity of liquids

Thermal Analysis, room N265

  • Thermal Gravimetric Analysis (TGA)
  • Differential Scanning Calorimetry (DSC): Stare System
  • Thermal analyser microcalorimetry: TAMIII
  • Optical hot stage microscopy with DSC82X system

Surface Measurement (Dynamic Vapour Sorption)

  • Dynamic Vapour Sorption: DVS Intrinsic, room S237a
  • Surface Energy Analyser: IGC SEA, room N265
  • Surface Area and Porosity: Tristar II, room S237a

Electronic Static Charge Analysis (Aerosel Characterization)

  • Electrical Low Pressure Impactor (ELPI): to measure the net electronic static charge of aerosols, room S211
  • BOLAR: to measure the individual electronic static charge of aerosols, room S206

Strength Characterization, room S211

  • Instron 3366: to quantify hardness of material by pressure
  • Indentor: to quantify hardness of material by pressure

Refractive Index Analysis, room S211

  • Thermo spectronic: to measure refractive index of solids and liquids

Dryers, Mixers and Accessories

  • Spray Buchi Mini spray dryer B290 with inert loop B-295, room S211
  • Christ alpha 1-4 Freeze drier, room S211
  • Spray freeze drying, room S211
  • multi-inlet vortex micro-mixer, room S211
  • Ultrasonic nozzle, room S211
  • Ultrasonic probe, room S211
  • Homogenizer, room S211
  • 3D printer, room S237a
  • PHD 2000 syringe pump, room S211

Structure Determination

Mass spectrometer

Thomas R Watson Mass Spectrometry Laboratory
TSQ Quantum Access Max (Triple Quadrupole) LCMS/MS & TLX1 Turboflow Chromatography System. Room S245.


NMR spectrometer

The facility has a new fully automated State of Art Spectrometer Varian Gemini 2000, a 400MR System with SMS Auto sampler. The System has Pulsed Field Gradients & PFG 5mm ATB Broadband Probe 1H/19F/X, the Magnet is a Premium Shielded, long hold magnet with up to 270 days Liquid Helium hold time.

This acquisition will enable researchers to carry out the latest experiments with minimal effort and with excellent sensitivity. Room S248.


X-Ray chararacterisation

The SuperNova (Dual) Diffractometer System combines the hi-flux micro-focus sources with one of the last, self-optimizing S2 CCD detectors. This combination of high intensity and fast, large active area CCD enables rapid data collection, with easy software-switching between molybdenum and copper wavelengths, for small molecule and protein crystallography. Room S237b.


Atomic Absorption Spectrometer

The AA-7000F Atomic Absorption Spectrophotometer features high-sensitivity analysis, flexible system configuration, and a compact footprint for user-friendly operation. Room S222.


Computing Biology

The Faculty supports the latest in software for computational drug design. The state-of-the-art Schrödinger suite covers small molecule drug discovery, the ability to design biologics, materials design and discovery informatics.

For high-end quantum chemical calculations the Faculty has Gaussian09, GAMESS and Jaguar packages.


Other Spectroscopies

Spectrofluorometer

  • RF-5310 PC, room N355
  • Fluorolog-3, room S206
  • time-resolved fluorescence, room S206

UV/Vis spectrophotometer

  • UV-2600 UV-Vis, rooms S206 and S211
  • UV-1800 UV-Vis, room N423
  • UVmini-1240 UV-Vis, rooms N355 and N431

Fourier transform infrared spectrometry

  • FTIR-8400s, room N355