Development of novel methods to characterize and quantify oxdiative stress in cells
Summary
This project will develop novel and advanced liquid chromatography-mass spectrometry (LC-MS) based assays to chemically define and quantify oxidative stress in diseases such as cardiovascular disease and cancer, using model cultured cells.
Supervisor(s)
Research Location
Bosch Institute (Vascular Immunology Unit)
Program Type
Masters/PHD
Synopsis
It is now well recognized that many diseases are associated with increased oxidative stress, and that different reactive oxygen species can have physiological and pathological roles, depending on the circumstances involved. For example, the interplay between activation of receptor tyrosine kinases and inhibition of protein tyrosine phosphatases is regulated tightly by redox-dependent posttranslational protein modifications. However, for many signaling systems, the molecular mechanism underlying such regulation remains elusive.
The aim of this project is to establish LC-MS based methods employing a range of novel probes that are specific for superoxide anion radical, hydrogen peroxide, peroxynitrite and hypochorous acid, and that can be targeted to specific cellular compartments, including mitochondria, the outer and inner plasma membrane, endoplasmic reticulum and nucleus. The methods to be developed will allow quantification of both parent and reacted probes. This includes the chemical characterization of the different products formed to facilitate the establishment of footprints for the different oxidants/probes involved. This project will provide the basis for a better understanding of redox-modulated pathways in health and disease.
A prerequisite for the successful undertaking of this project is a sound knowledge in chemistry, and experience in analytical chemistry, particularly LC and/or MS is advantageous.
Additional Information
- The opportunity is available primarily for prospective PhD students Honours degree is required. Applicant is expected to apply for PhD scholarship.
- Other PhD and Honours Projects are available, or can be negotiated.
- The project may be adjusted to an Honours Project.
For additional information on the Stocker Laboratory and its current members, including students, see: http://www.bosch.org.au/research/Cardiovascular/VascularResearch/index.php
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Keywords
oxidative stress, cardiovascular disease, Cancer
Opportunity ID
The opportunity ID for this research opportunity is: 1024
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