The molecular basis of persistence in clinical P. aeruginosa strains from cystic fibrosis patients

Summary

Pseudomonas aeruginosa, the leading cause of morbidity and mortality in people with cystic fibrosis (CF), adapts to survive in the CF lung through both mutation and gene expression changes

Supervisor(s)

Dr Jim Manos, Dr Jamie Triccas

Research Location

Camperdown - Central Clinical School

Program Type

PHD

Synopsis

Frequent clonal strains found in multiple patients, such as the Australian Epidemic Strain-1 (AES-1), have increased ability to establish infection in the CF lung and to superimpose and replace infrequent clonal strains. Meanwhile some frequent and infrequent clonal strains persist to chronic infection while others do not
Little is known about the factors underpinning these properties. We have used a non-redundant array of eight P. aeruginosa genomes and a media mimicking CF sputum to establish the conserved cohort of differentially expressed genes in persistent clinical isolates. Preliminary deletion mutants of genes have been made and the effects are being tested in a mouse model of lung infection. The aim here is to mutate large numbers of genes simultaneously and particularly in the clinical isolates rather than laboratory strains and establish which genes play a role in persistence

Additional Information

The successful PhD candidate should have good experience in cloning and mutation of P. aeruginosa genes and also in the making of transposon libraries

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Keywords

Cystic fibrosis, Pseudomonas aeruginosa, lung infection, Infectivity, virulence genes, genomic variability, attenuated strain, Cardiovascular & respiratory diseases, Chronic diseases & ageing, Infectious diseases, Infection & immunity, Respiration

Opportunity ID

The opportunity ID for this research opportunity is: 1314

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