The influence of genetic variations of human organic anion transporting polypeptides in drug disposition

Summary

The research in our group is aimed to understand the influence of natural variations of human transporter genes on drug performance, which will largely impact on the therapeutic outcome of the disease treatment.  

Supervisor(s)

Dr Fanfan Zhou, Professor Michael Murray

Research Location

Pharmacy Research Theme - Cancer

Program Type

Masters/PHD

Synopsis

Human organic anion transporting polypeptides (OATPs) are important transporters that modulate the cellular influx of endogenous substances and many clinically important drugs. OATP dysfunction may have serious consequences for human health by altering homeostasis and impairing drug efficacy and disposition. It has been proved that OATPs can interact with many anti-cancer agents. We recently identified several novel variants of a particular OATP protein in cancer patients who received an anti-cancer agent. It is also know that the OATP protein that we are interested with is responsible for the cellular uptake of this agent. The present project will evaluate the underlying molecular mechanisms whereby these genetic variations impact on transporter function. This study will build on our previous findings to define the mechanisms that human genetic variations influence transporter function, therefore, impact on the outcome of drug therapy. This pharmacogenomic information could then be utilised to tailor the selection of drug and its dosage to the individual in the future.

Additional Information

The techniques involved in this project include:
PCR
Genotyping
Western blotting
Gene Cloning and site-directed mutagenesis
Plasmid/genomic DNA preparation
Cell culturing techniques including gene transfection
Immunohistochemistry/immunostaining
Confocol microscopy

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Keywords

solute carrier transporters, drug disposition, transporter pharmacogenomics, drug transporters, transporters

Opportunity ID

The opportunity ID for this research opportunity is: 1574

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