Cyanobaterial photoregulatory mechanisms, pigmentation varieties and their evolutionary significance
The red-shifted chlorophylls contain an extra formyl group compared to chlorophyll a in cyanobacteria. The oxygen atom in the formyl group requires oxygen molecules as one important reactant. Understanding the light regulatory mechanism for biosynthesis of red-shifted chlorophylls will enhance our knowledge of oxygenic photosynthesis driven by red-shifted chlorophylls and will extend the potential application of red-shifted chlorophylls.
Photosynthesis is the most important reaction on earth. Oxygenic photosynthesis evolved ~ 3 billion years ago, and is responsible for increasing/maintaining the free oxygen level in atmosphere/biosphere. Oxygen is the key element for evolving the advanced life forms as what we have today. Cyanobacteria are the only bacteria that can use light energy for splitting water and evolving oxygen. They have great capability to adapt to different environments through changing the composition of pigmentation and metabolic pathways. This project will focus on understanding the photoregulatory mechanism in cyanobacteria. Two main elements: Light and oxygen levels, play important roles for photoregulatory strategies. Characterisation of light-sensory processes in cynaobacteria in response to different oxygen levels will provide us the knowledge basis for identifying the photoregulatory mechanism of pigmentation and photopigment biosynthesis.
The approaches for this project include gene modification technology, transcriptional regulation and the biochemical/biophysical characterisation, liquid chromatographic and spectral analysis.
• Current PhD/Hons topics being undertaken at the location or with the supervisors
Three PhD Projects are being undertaken in A/Prof Chen’s laboratory.
1. Light-harvesting systems in Chromera velia
2. Function of antenna systems in a newly isolated cyanobacterium containing chlorophyll f
3. Global protein analysis of cyanobacterium Acaryochloris marina under various oxygen-stressed conditions.
• Is the opportunity also available for Honours students?
Yes, one-year potential projects are available for honours students. Details please contact A/Prof Min Chen (firstname.lastname@example.org)
• Techniques, methodologies, research approaches, technologies, etc., employed by the project - e.g., electron microscopy, textual analysis, etc.
Pigment and pigment-bound protein analyses are performed by using a UV/Vis spectrophotometer, fluorescence spectrophotometer and other molecular spectral analysis methods.
General protein isolation and characteristic methods, such as electrophoresis (SDS-PAGE, IEF, Western Blotting, Native electrophoresis, 2-D gel, peptide mass fingerprinting and other proteomic analysis.
Chromatographic analysis such as HPLC (high-performance liquid chromatography), FPLC (Fast protein liquid chromatography), gel filtration and ion-exchanging columns for proteins and protein-complexes purification.
DNA, RNA isolation, PCR (DNA as templates) and RT-PCR (RNA as templates), Gene transformation and functional studies in vitro.
General biochemical and molecular biological experiences are required for potential students who want to study inthe laboratory. Hons A or similar experiences is required.
• Scholarships/funding available
ARC Centre of Excellent for Translational Photosynthesis (2014-2020)
Biosynthesis of chlorophylls (ARC Future Fellow, 2013-2016)
ARC Discovery Project (2012-2014)
Want to find out more?
Photosynthesis, evolution of oxygenic photosynthesis, chlorophyll, light-harvesting complexes, phycobiliproteins, chlorophyll-binding protein complexes, proteomics of membrane-bound protein complexes, Protein structural models. Stress-response plant physiology (light, oxygen and nutrients), biosynthesis of chlorophyll and other photopigments. Acaryochloris, blue-green algae, cyanobacteria, Bioinformatics and functional genomics, hongdechloris
The opportunity ID for this research opportunity is: 1865
Other opportunities with Professor Min Chen
- Evolutionary relationships of aerobic and anaerobic metabolic reactions
- The substitution and formation of red-shifted chlorophylls
- Spectral extension in photosynthesis: molecular mechanism of photosynthesis driven by red-shifted chlorophylls
- Molecular mechanism of photo-regulation in cyanobacteria
- Developing a pathway to incorporate red-shifted chlorophylls into light-harvesting complexes to extend the solar spectrum in photosynthesis
- Light-harvesting complexes: adaptation and efficiency