Evolutionary relationships of aerobic and anaerobic metabolic reactions
Novel pigments and new direction in photosynthesis: past, now and future
This project includes two parts, biochemical analysis of enzyme activities under various oxygen concentrations and in silico analysis of relationships of key domains that catalyse similar reactions but have different oxygen level requirements. Understanding what metabolic changes occurred in response to the shift in environment (such as oxygen level requirement) will have evolutionary implication in different fields. Two stages will be included to complete this project: Stage 1, Identification, classification and evolutionary analyses of interesting genes using bioinformatics; Stage 2, Experimental tests using integrating methods of molecular biology and biochemistry.
A PhD candidate whose project is the investigation of intermediate products in the late stage of chlorophyll biosynthesis. His project involves: to isolation and identify hydrophilic intermediate chlorophyll-like pigments using HPLC, Mass Spectrometry and NMR structure analysis, to survey the novel pigments from unique environments (such as Great Barrier Reef, Australia) by using specific light emitting diodes (LED) with different light wavelength.
Is the opportunity also available for Honours students?
Yes, one-year potential projects are available for honours students. Details please contact Dr Min Chen (email@example.com)
Pigment and pigment-bound protein analyses are performed by using spectrophotometer, fluorescence photometer and other molecular spectral analyzing methods.
General protein isolation and characteristic methods, such as electrophoresis (SDS_PAGE, IEF, Native electrophoresis, 2-D gel, peptide mass finger printing and other proteomic analysis, protein structure (electromicroscope image analysis and single particle modeling).
Chromagraphic anaylssis such as HPLC (high-performance liquid chromagraphy), FPLC (Fast protein liquid chromagraphy), gel filtration and ion-exchanging columns for sample purification. Centrifugation and gradient centrifugation will use to obtain different size of protein complexes.
DNA, RNA isolation, PCR (DNA as templates) and RT-PCR (RNA as templates), Gene transformation and functional studies in vitro.
Current two ARC discovery projects will support the research in my laboratory to 2012.
General biochemical and molecular biological experiences are required for potential students who want to study in my laboratory. Hons A or similar experiences is required.
Want to find out more?
Photosynthesis, evolution of oxygenic photosynthesis, chlorophyll, light-harvesting complexes, phycobiliproteins, chlorophyll-binding protein complexes, proteomics of membrane-bound protein complexes, Protein structural models. Stress-response plant physiology (light, oxygen and nutrients), biosynthesis of chlorophyll and other photopigments. Acaryochloris, blue-green algae, cyanobacteria, evolution of cyanobacteria. Biogeographics, Bioinformatics and functional genomics
The opportunity ID for this research opportunity is: 776
Other opportunities with Associate Professor Min Chen