Academic Staff - Dr Bruce Lyon

Bruce Lyon Photo
Position: Senior Lecturer  
Phone: +61 2 9351 4240
Fax: +61 2 9351 4771
Mobile Phone: N/A
Email: brucel@bio.usyd.edu.au
Location: Room 239a
Address: A12 - Macleay, The University of Sydney, NSW 2006 Australia 
Links: N/A


Areas of Interest

My research interests encompass the molecular genetics of microbial pathogenesis in animals and plants.

We aim to isolate and characterise genes from bacterial and fungal pathogens that are implicated in the infectious process. This involves pathogenicity assays, genetic fingerprinting, PCR amplification of selected genes, DNA sequencing, bioinformatic and genomic analysis and gene expression studies. In part, these results are used to promote new identification aids and to provide insights into epidemiology and taxonomy. We also employ molecular tools to explore the evolution of the gene transfer mechanisms that have equipped pathogens to overcome host defence responses and to defeat antimicrobial therapies.


Research Output

  • Lyon BR, JW May, JH Marshall, and RA Skurray (1982). Plasmid-mediated antibiotic resistance in methicillin-resistant Staphylococcus aureus. Med. J. Aust. 1: 468-469
  • Lyon BR, JW May, and RA Skurray (1983). Analysis of plasmids in nosocomial strains of multiple-antibiotic resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 23: 817-826.
  • Lyon BR, JL Iuorio, JW May, and RA Skurray (1984). Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. J. Med. Microbiol. 17: 79-89.
  • Lyon BR, JW May, and RA Skurray (1984). Tn4001 : a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Mol. Gen. Genet. 193: 554-556.
  • Tennent JM, BR Lyon, MT Gillespie, JW May, and RA Skurray (1985). Cloning and expression of Staphylococcus aureus plasmid-mediated quarternary ammonium resistance in Escherichia coli. Antimicrob. Agents Chemother. 27: 79-83.
  • Lyon BR, JM Tennent, JW May, and RA Skurray (1986). Trimethoprim resistance encoded on a S. aureus gentamicin resistance plasmid: cloning and transposon mutagenesis. FEMS. Microbiol. Lett. 33: 189-192.
  • Lyon BR, MT Gillespie, ME Byrne, JW May, and RA Skurray (1987). Plasmid-mediated resistance to gentamicin in Staphylococcus aureus : the involvement of a transposon. J. Med. Microbiol. 23: 101-110.
  • Lyon BR, and R. Skurray (1987). Antimicrobial resistance of Staphylococcus aureus : genetic basis. Microbiol. Rev. 51: 88-134.
  • Gillespie MT, BR Lyon, LSL Loo, PR Matthews, PR Stewart, and RA Skurray (1987). Homologous direct repeat sequences associated with mercury, methicillin, tetracycline and trimethoprim resistance determinants in Staphylococcus aureus. FEMS Microbiol. Lett. 43: 165-171.
  • Gillespie MT, BR Lyon, LJ Messerotti, and RA Skurray (1987). Chromosome- and plasmid-mediated gentamicin resistance in Staphylococcus aureus encoded by Tn4001. J. Med. Microbiol. 24: 139-144.
  • Lyon BR, MT Gillespie, and RA Skurray (1987). Detection and characterization of IS256, an insertion sequence in Staphylococcus aureus. J. Gen. Microbiol. 133: 3031-3038.
  • Tennent JT, H-K Young, BR Lyon, SGB Amyes, and RA Skurray (1988). Trimethoprim resistance determinants encoding a dihydrofolate reductase in clinical isolates of Staphylococcus aureus and coagulase negative staphylococci. J. Med. Microbiol. 26: 67-73.
  • Sinclair MI, PC Maxwell, BR Lyon, and BW Holloway (1986). Chromosomal location of TOL plasmid DNA in Pseudomonas putida. J. Bacteriol. 168: 1302-1308.
  • Holloway BW, PP Kearney, and BR Lyon (1987). The molecular genetics of C1 utilizing microorganisms - an overview. Antonie van Leeuwenhoek 53: 47-53.
  • Lyon BR, PP Kearney, MI Sinclair, and BW Holloway (1988). Comparative complementation mapping of Methylophilus spp. using cosmid gene libraries and prime plasmids. J. Gen. Microbiol. 134: 123-132.
  • Skurray RA, DA Rouch, BR Lyon, MT Gillespie, JM Tennent, ME Byrne, LJ Messerotti, and JW May (1988). Multiresistant Staphylococcus aureus : genetics and evolution of epidemic Australian strains. J. Antimicrob. Chemother. 21(Suppl.C, 19-38).
  • Gillespie MT, BR Lyon, and RA Skurray (1988). Structural and evolutionary relationships of ß-lactamase transposons from Staphylococcus aureus. J. Gen. Microbiol. 134: 2857-2866.
  • Tennent JM, BR Lyon, M Midgley, IG Jones, AS Purewal, and RA Skurray (1989). Physical and biochemical characterization of the qacA gene encoding antiseptic and disinfectant resistance in Staphylococcus aureus. J. Gen. Microbiol. 135: 1-10.
  • Mahairas GG, BR Lyon, RA Skurray, and PA Pattee (1989). Genetic analysis of Staphylococcus aureus with Tn4001. J. Bacteriol. 171: 3968-3972.
  • Gillespie MT, BR Lyon, and RA Skurray (1989). Gentamicin and antiseptic resistance in epidemic methicillin-resistant Staphylococcus aureus. Lancet i: 503.
  • Lyon BR, DJ Llewellyn, JL Huppatz, ES Dennis, and WJ Peacock (1989). Expression of a bacterial 2,4-D herbicide degradation gene in transgenic tobacco plants confers resistance to 2,4-D. Plant Mol. Biol. 13: 533-540.
  • Gillespie MT, BR Lyon, and RA Skurray (1990). Typing of methicillin-resistant Staphylococcus aureus by antibiotic resistance phenotypes. J. Med. Microbiol. 31: 57-64.
  • Llewellyn D, BR Lyon, Y Cousins, J Huppatz, ES Dennis, and WJ Peacock (1990). Genetic engineering of plants for resistance to the herbicide 2,4-D. In "Genetic Engineering of Crop Plants". (GW Lycett and D Grierson, eds.) Butterworths, London.
  • Lyon, BR (1991). Engineering microbial herbicide detoxification genes in higher plants. In "Advances in Plant Gene Research Vol.7 - Molecular Approaches to Crop Improvement". pp. 79-108 (ES Dennis, DJ Llewellyn eds.) Springer-Verlag, Vienna.
  • Cousins YL, BR Lyon, and DJ Llewellyn (1991). Transformation of an Australian cotton cultivar: prospects for cotton improvement through genetic engineering. Aust. J. Plant Physiol. 18: 481-494.
  • Lyon BR, YL Cousins, DJ Llewellyn, and ES Dennis (1993). Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid. Transgenic Research 2: 162-169.
  • Llewellyn D, Y Cousins, A Mathews, L Hartweck, and B Lyon (1994). Expression of Bacillus thuringiensis insecticidal protein genes in transgenic crop plants. Agriculture, Ecosystems and Environment 49: 85-93.
  • Multani DS, and BR Lyon (1995). Genetic fingerprinting of Australian cotton cultivars with RAPD markers. Genome 38: 1005-1008.
  • Silva WPK, DS Multani, BJ Deverall, and BR Lyon (1995). RFLP and RAPD analyses in the identification and differentiation of isolates of the leaf spot fungus Corynespora cassiicola. Aust. J. Botany 43: 609-618.
  • Ridgway KP, MK Hill, JL Morrow, PA McGee and BR Lyon (1996). Identification of symbiosis-related genes in the cotton - arbuscular mycorrhiza association. In “Mycorrhizas in Integrated Systems from Genes to Plant Development. Proceedings of the Fourth European Symposium on Mycorrhizas.” pp. 199-202 (C. Azcon-Aguilar, JM Barea eds.).
  • Ramsay JR, DS Multani, and BR Lyon (1996). RAPD-PCR identification of Verticillium dahliae isolates with differential pathogenicity on cotton. Aust. J. Agric. Res. 47: 681-93.
  • McMaugh SJ, and BR Lyon (1997). Molecular genetic analysis of the defensive responses of Cynodon dactylon to the fungal pathogen Leptosphaeria narmari. International Turfgrass Soc. Res. J. 8: 1166-1175.
  • Silva WPK, BJ Deverall, and BR Lyon (1998). Molecular, physiological and pathological characterisation of Corynespora leaf spot fungi from rubber plantations in Sri Lanka. Plant Pathol. 47: 267-277.
  • Hill MK, KJ Lyon, and BR Lyon (1999). Identification of disease response genes expressed in Gossypium hirsutum upon infection with the wilt pathogen Verticillium dahliae. Plant Mol. Biol. 40: 289-296.
  • Zhu YY, YY Wang, DS Multani, and BR Lyon (1999). The relationships between DNA polymorphism and geographical origin of Australian isolates of Verticillium dahliae from cotton plants. Mycosystema 18: 366-373.
  • Zhu YY, YY Wang, and BR Lyon (1999). PCR detection of Verticillium dahliae from diseased plants. Acta Phytopathologica Sinica 29: 250-255.
  • Rungis D, D Llewellyn, ES Dennis, and BR Lyon (2002). Investigation of the chromosomal location of the bacterial blight resistance gene present in an Australian cotton (Gossypium hirsutum L.) cultivar. Aust. J. Agric. Res. 53: 551-560.
  • McMaugh SJ, and BR Lyon (2003). Real-time quantitative RT-PCR assay of gene expression in plant roots during fungal pathogenesis. BioTechniques 34: 982-986.
  • Rungis D, D Llewellyn, ES Dennis, and BR Lyon (2005). Simple sequence repeat (SSR) markers reveal low levels of polymorphism between cotton (Gossypium hirsutum L.) cultivars. Aust. J. Agric. Res. 56: 301-307.
  • Delannoy E, BR Lyon, P Marmey, A Jalloul, JF Daniel, JL Montillet, M. Essenberg, and M Nicole (2005). Resistance of cotton towards Xanthomonas campestris pv. malvacearum. Annu. Rev. Phytopathol. 43: 63-82.
  • Becerra-LopezLavalle LA, JA Saleeba, and BR Lyon (2005). Molecular identification of fungi isolated from stem tissue of Upland cotton (Gossypium hirsutum L.). Aust. J. Botany 53: 571-578.
  • Palmer CS, JA Saleeba and BR Lyon (2006). Phytotoxicity on cotton ex-plants of an 18.5 kDa protein from culture filtrates of Verticillium dahliae. Physiol. Mol. Plant Pathol. 67: 308-318.