The Molecular Genetics Lab - Facilities

Main Lab & Equipment Rooms

The Molecular Genetics Laboratory is extremely well equipped for contemporary molecular biology, genetics, cellular, and bioinformatics research, and this is supplemented by conveniently located shared instrument facilities containing major equipment items. Molecular Genetics Lab staff and students also have access to an office area with networked computers and wireless internet, as well as state-of -the-art kettle, toaster, fridge, microwave and sink!

The Molecular Genetics Laboratory is a purpose-built Physical Containment Level 2 research facility with integrated equipment rooms. Major items of equipment housed in the lab include:

  • Bio-Rad Gene Pulsor electroporation unit with Pulse Controller
  • Pharmacia FPLC system
  • Beckman Oligo 1000 DNA synthesiser
  • Millipore Milli-Q water purification system
  • BIO101 FastPrep cell disruptor
  • Pharmacia semi-dry trans-blot cells
  • Bio-Rad iCycler thermal cycler with gradient sample block
  • Hybaid PCR Express thermal cycler with gradient sample block
  • Hybaid PCR Sprint thermal cycler
  • MJ Research PTC-100 thermal cycler with Hot Bonnet
  • Perkin Elmer Cetus 480 thermal cycler
  • Extron hybridisation oven
  • Bio-Rad horizontal agarose gel electrophoresis equipment
  • Bio-Rad Protean II and mini-Protean vertical polyacrylamide gel electrophoresis equipment for SDS PAGE and trans-blotting
  • Bio-Rad and BRL DNA sequencing cells
  • Bio-Rad, Pharmacia and E-C power supplies
  • Gallenkamp orbital incubator
  • Thermoline laboratory incubator and oven
  • Grant water baths
  • New Brunswick Scientific gyratory water bath
  • Email Westinghouse Class II biological safety cabinet
  • Dynaflow fume cupboard
  • Revco and Sanyo ultralow freezers
  • DynaVac freeze dryer
  • Savant SpeedVac concentrator
  • Napco vacuum oven
  • Hoefer slab gel dryer
  • A&D balances
  • Ratek heating blocks
  • Ratek vortex mixers
  • Ratek rotary tube mixer
  • Ratek orbital platform
  • BioLine platform rocker
  • Watson Marlow dispensing pump
  • 3 networked personal computers and laser printer
  • Beckman XL-90 ultracentrifuge with NVT90, VTi50, SW40Ti and SW55Ti rotors
  • Beckman J2-21M/E high-speed centrifuge with JA14 and JA20 rotors
  • Haereus Primo biofuge
  • Haraeus Pico biofuge
  • Atherton laboratory autoclave

Constant Temperature Rooms & A12 Molecular Analysis Facility

Storage and growth facilities consist of self-contained 4ûC and 37ûC rooms located adjacent to the lab.

The cold room contains:

  • Haereus Primo biofuge
  • Haraeus Pico biofuge
  • Ratek rotary tube mixer

The warm room contains:

  • New Brunswick Scientific gyratory shaker

We are also major users of the A12 Molecular Analysis Facility that is also located adjacent to the Molecular Genetics Lab, which contains the following instruments:

  • Bio-Rad Molecular Imager FX with external laser unit
    Corbett Research Rotor-Gene RG3000 real-time PCR machine
    Bio-Rad ChemiDoc
  • Bio-Rad GS710 calibrated imaging densitometer
  • Bio-Rad Benchmark microplate reader
  • Hitachi fluorescence spectrophotometer
  • Shimadzu UV/Vis spectrophotometer
  • Pharmacia QuantaGene spectrophotometer
  • Packard liquid scintillation counter
  • Sim-Aminco French press
  • Branson sonifier

An integrated darkroom contains:

  • Hoefer UV transilluminator
  • Polaroid MP4 Land Camera
  • AGFA automated X-ray processor

A12 Microscopy Facility

We also make extensive use of the A12 Microscopy Facility for our cell biology analyses, which include immunofluorescence microscopy (IFM) and Green Fluorescent Protein (GFP) fusion studies.

The facility contains:

  • Zeiss Axiophot microscope with a Roper Scientific CoolSnap HQ high sensitivity CCD camera
  • Computer with MetaMorph software