Metabolomics

The metabolomics lab is equipped with state-of-the-art instrumentation for separations and metabolite detection/identification. The lab’s main analytical platforms are LC-MS, CE-MS and GC-MS. For data analysis we have mass spectral libraries, an online database (EMIS), and specialist software for multivariate statistics.

Success stories & firsts

Notable success stories in the first few years of the lab’s operation include:

Services available

 GC-MS

Cost* ($AUD/sample) 

Targeted analysis of amino acids/amines (~25 common amino acids plus others on request)

 35
Targeted analysis of carbohydrates (mono-, di- and tri-saccharides)  35
Untargeted metabolite profiling (amino acids, organic acids, sugars, sugar alcohols, sugar phosphates, cyclic sugars etc.)  45

 LC-MS

Cost* ($AUD/sample) 
Chiral separation of amino acid enantiomers (chirobiotic stantionary phase)     please enquire
CE-MS

Cost*

($AUD/sample)

Profiling of 100 small organic N compounds (amino acids, polyamines, nucleobases, betaines)

please enquire
MS and MS/MS of hydrophilic peptides please enquire

*Costs are indicative values applicable to academic customers with non-commercial samples. Minimum sample numbers apply. Costs are charged irrespective of results. Please e-mail Assoc Prof Charles Warren to find out more about services and costs.

Analytical workflow

A simplified generic workflow for untargeted metabolomics is shown in the figure below. Note that to obtain a broad coverage of the metabolome we commonly use multiple mass spectrometry platforms (e.g. LC-MS, GC-MS and CE-MS) in combination with multiple separation modes (e.g. CE-MS of anions, CE-MS of cations) and derivatization schemes.

generic metabolomics workflow

Metabolomics workflow (from Lee et al 2010 BioTechniques 49:557–565)