Metabolomics

The metabolomics lab is equipped with state-of-the-art instrumentation for separations and metabolite detection/identification. The lab’s two main analytical platforms are CE-MS and GC-MS. For data analysis we have mass spectral libraries, an online database (EMIS), and specialist software for multivariate statistics.

Success stories & firsts

Notable success stories in the first few years of the lab’s operation include:

Services available

 GC-MS

Cost* ($AUD/sample) 

Targeted analysis of amino acids/amines (~25 common amino acids plus others on request)

 35
Targeted analysis of carbohydrates (mono-, di- and tri-saccharides)  35
Targeted analysis of FAMES  please enquire
Untargeted metabolite profiling (amino acids, organic acids, sugars, sugar alcohols, sugar phosphates, cyclic sugars etc.)  45
Biomarker compounds (e.g. D/L amino acids, muramic acid, aminosugars, ergosterol)  please enquire
CE-MS

Cost*

($AUD/sample)

Profiling of cations

please enquire
Profiling of anions please enquire
MS and MS/MS of peptides please enquire
Metabolic fingerprinting by direct infusion MS please enquire

*Costs are indicative values applicable to academic customers with non-commercial samples. Minimum sample numbers apply. Costs are charged irrespective of results. Please e-mail Assoc Prof Charles Warren to find out more about services and costs.

Analytical workflow

A simplified generic workflow for untargeted metabolomics is shown in the figure below. Note that to obtain a broad coverage of the metabolome we commonly use multiple mass spectrometry platforms (e.g. GC-MS and CE-MS) in combination with multiple separation modes (e.g. CE-MS of anions, CE-MS of cations) and derivatization schemes.

generic metabolomics workflow

Metabolomics workflow (from Lee et al 2010 BioTechniques 49:557–565)