The metabolomics lab is equipped with state-of-the-art instrumentation for separations and metabolite detection/identification. The lab’s two main analytical platforms are CE-MS and GC-MS. For data analysis we have mass spectral libraries, an online database (EMIS), and specialist software for multivariate statistics.
Success stories & firsts
Notable success stories in the first few years of the lab’s operation include:
- First identification and quantification of peptides in soil (APCE 2011,Soil Biology and Biochemistry 63: 80-84)
- Development of analytical methods for comprehensive profiling of small organic N compounds in soil by CE-MS (Soil Biology and Biochemistry 57:444-450) and LC-MS (Soil Biology and Biochemistry 78: 233-242)
- Evaluation of methods for extracting organic N from the soil microbial biomass(Soil Biology and Biochemistry 81: 67-76)
- First comprehensive profiling of polar metabolites in the model tree species Eucalyptus (Metabolomics 8:186–200)
- Discovery of two metabolites responsible for osmotic adjustment in Acacia spp. that were overlooked by the previous 30+ years of research (Plant, Cell and Environment 34: 1609–1629)
- First GC-MS mass spectral database for methane and ammonia chemical ionization (Metabolomics 9: 110-120)
Targeted analysis of amino acids/amines (~25 common amino acids plus others on request)
|Targeted analysis of carbohydrates (mono-, di- and tri-saccharides)||35|
|Targeted analysis of FAMES||please enquire|
|Untargeted metabolite profiling (amino acids, organic acids, sugars, sugar alcohols, sugar phosphates, cyclic sugars etc.)||45|
|Biomarker compounds (e.g. D/L amino acids, muramic acid, aminosugars, ergosterol)||please enquire|
Profiling of cations
|Profiling of anions||please enquire|
|MS and MS/MS of peptides||please enquire|
|Metabolic fingerprinting by direct infusion MS||please enquire|
*Costs are indicative values applicable to academic customers with non-commercial samples. Minimum sample numbers apply. Costs are charged irrespective of results. Please e-mail Assoc Prof Charles Warren to find out more about services and costs.
A simplified generic workflow for untargeted metabolomics is shown in the figure below. Note that to obtain a broad coverage of the metabolome we commonly use multiple mass spectrometry platforms (e.g. GC-MS and CE-MS) in combination with multiple separation modes (e.g. CE-MS of anions, CE-MS of cations) and derivatization schemes.