Separations facility

The Separations (Chromatography) Facility has; two LCMSs, nine HPLCs, two GCs that are used for analytical and preparative chromatographic separations of organic (non-biological) samples.

High Performance Lipid Chromatography (HPLC)

Nine HPLC systems are available for the separation of organic (non-biological) mixtures in quantities ranging from analytical (less than 1 mg) up to preparative (greater than 200 mg) scale. Typically C18 reverse phase columns are used; brief description of each system is set out below.

  1. Waters™Alliance analytical HPLC; Automatic injection, quaternary gradient, analytical scale system with PDA and ELSD detection.
  2. Waters™600; Semi-preparative/preparative HPLC, quaternary gradient, with UV detector.
  3. Waters™500; analytical HPLC, isocratic system dedicated for chirial analysis, with RI and dual wavelength UV detector.
  4. Waters™500; analytical HPLC with autosampler, binary gradient system with UV detection.
  5. Waters™600; analytical/semi-preparative/preparative HPLC, quaternary gradient, with PDA detector.
  6. Amersham Äkta system, binary low pressure gradient, analytical/semi-preparative with UV, conductivity and pH monitoring.
  7. Waters™2525 preparative auto purification system. Automatic injection, binary high pressure gradient with PDA detection and fraction collection.
  8. Waters™2535 preparative HPLC with dual wavelength UV detector.
  9. Waters™2535 preparative HPLC with dual wavelength UV detector.

Liquid Chromatography-Mass Spectroscopy (LCMS)

These LCMS system are for the high throughput analysis of HPLC fraction

  1. Thermo-Finnigan LCQ DECA system, automatic injection, quaternary gradient, analytical with PDA and ion trap mass spec detection.
  2. Shimadzu 2020 LCMS system, automatic injection, quaternary gradient, analytical with PDA and ESI detectors.

Gas Chromatography (GC)

  1. Dedicated for the analysis of small chirial molecules using an FID detector.
  2. For determination of organic mixtures using an FID detector.


The School uses the microanalysis services of the Campbell Microanalytical Laboratory within the Department of Chemistry at the University of Otago, New Zealand. To access this, you need to follow the following procedures.

Procedure for submission of Microanalysis samples to the Campbell Microanalytical Laboratory

  • Make sure that your samples are pure, dry and free of all extraneous material (boiling chips, aluminium foil, dust, etc.). Transfer your samples to suitable containers and label them with unique code names.
  • Fill out a Microanalysis Request Form for each of your samples. For most samples, C,H,N analysis is sufficient. You can request the analysis of other elements, but additional sample is required and an additional cost is incurred.
  • Fill out the Microanalysis Order Form, print it and obtain your supervisor’s signature. Give the form to Lisa and ask her for the Order Number as you will need to quote it in the documentation that accompanies your sample.
  • Complete the Microanalysis Documentation Form. Print the form and sign it.
  • Access the AQIS form and fill in the current date, any additional information necessary, your name and email address. After you have printed the form, sign it.
  • Place your samples in a sturdy box with sufficient padding to prevent movement during the transport to New Zealand. Make sure you place in the box all Microanalysis Request forms as well as the documentation form with details of your order. Secure the box with tape, place the AQIS form on top of the container, wrap the parcel with brown paper, attach an airmail sticker and fill in the address details as follows:

    Campbell Microanalytical Laboratory
    Department of Chemistry
    University of Otago
    P.O. Box 56, Dunedin
    New Zealand

    At the Post Office you will need to complete a customs declaration, which will be attached to the parcel by the staff, and show some form of photo I.D. (Driver Licence). On the Customs Declaration, make sure that you fill in the field labelled Detailed description of contents with the following words:

    “Synthetic research samples. NOT of plant or animal origin.”
    Any other description could result in confiscation of your valuable samples.
  • Obtain a receipt from Australia Post so you can be reimbursed from petty cash for the cost of the airmail postage.

Chromatography Facility instrument booking procedures

School of Chemistry users, please login via the HPLC Separation Facility website.

Outside users, please contact .

Note: All users must be trained and certified before use.


Dr Cody Szczepina
Professional Officer
School of Chemistry
The University of Sydney
NSW 2006 Australia
Room F11
T: +61 2 9114 1191