instruments



HPLC

Nine HPLC systems are available for the separation of organic (non-biological) mixtures in quantities ranging from analytical (less than 1 mg) up to preparative (greater than 200 mg) scale. Typically C18 reverse phase columns are used; brief description of each system is set out below.

  1. Waters™ Alliance analytical HPLC; Automatic injection, quaternary gradient, analytical scale system with PDA and ELSD detection.
  2. Waters™ 600; Semi-preparative/preparative HPLC, quaternary gradient, with UV detector.
  3. Waters™ 500; analytical HPLC, isocratic system dedicated for chirial analysis, with RI and dual wavelength UV detector.
  4. Waters™ 500; analytical HPLC with autosampler, binary gradient system with UV detection.
  5. Waters™ 600; analytical/semi-preparative/preparative HPLC, quaternary gradient, with PDA detector.
  6. Amersham Äkta system, binary low pressure gradient, analytical/semi-preparative with UV, conductivity and pH monitoring.
  7. Waters™ 2525 preparative auto purification system. Automatic injection, binary high pressure gradient with PDA detection and fraction collection.
  8. Waters™ 2535 preparative HPLC with dual wavelength UV detector.
  9. Waters™ 2535 preparative HPLC with dual wavelength UV detector.


LCMS

These LCMS system are for the high throughput analysis of HPLC fraction

  1. Thermo-Finnigan LCQ DECA system, automatic injection, quaternary gradient, analytical with PDA and ion trap mass spec detection.
  2. Shimadzu 2020 LCMS system, automatic injection, quaternary gradient, analytical with PDA and ESI detectors.

GC

The laboratory operates two Hewlett-Packard 5890 Series II GCs.

  1. Dedicated for the analysis of small chirial molecules using an FID detector.
  2. For determination of organic mixtures using an FID detector.