NMR Spectrometer

The School houses three high-field Bruker NMR spectrometers, an Avance III-400 widebore, an Avance III-600 and an Avance III-800, as well as computing facilities for the processing and analysis of NMR data. Both of the 600 and 800 NMR are equipped with cryogenic probeheads.

The four-channel Avance III-600 and 800 are each equipped with a triple resonance (1H/13C/15N; TCI) cryoprobe with a z-gradient and is mainly used for structure determination of proteins and nucleic acids as well as metabonomics studies. The three-channel Avance III 400 widebore spectrometer has a high power (1000 gauss/cm) diffusion probe-head and micro-imaging capabilities, as well as two dual (1H/13C and 1H/19F), three-axis gradient triple resonance (1H/13C/15N; TXI), and broadband multinuclear (BBO) probe-heads. This spectrometer is principally used for studies of the biophysical properties of cells, bacterial pathogenesis and characterisation of the mobility of biophysically significant molecules.

These facilities are managed by Dr Ann Kwan, and used primarily by the research groups of Prof. Philip Kuchel (molecular diffusion, metabolic kinetics, membrane transport, and peptide structure), Prof. Joel Mackay (protein/nucleic acids structure and interactions), A/Prof. Jacqui Matthews (protein structure) and Dr Ann Kwan with Dr Margaret Sunde (protein structure and molecular assembly).

 nmr_inprogress  

Ribbon representations of the structure of p22HBP, a heme-binding protein from mammalian erythrocytes, and of defensin-like-peptide 1 (DLP-1) from the venom of the Australian platypus.