Protein structure, function and engineering lab Publications of Prof Joel Mackay
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Recent news

25 July, 2014: Jacqui invited to speak at the triennial GATA meeting in 2015

1 May, 2014: Marylène's paper on designer RNA-binding proteins has been accepted in Angew. Chem.

1 May, 2014: Soumya is given the nod



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[Publication highlights]

[Joel - all papers] [Jacqui - all papers]

Refereed journal articles
  1. Mackay, J.P. (2014)
    Transcription factor seeks DNA – cognate site preferred
    J Mol Biol in press,

  2. Dickson, C.F., Kumar, K.K., Jacques, D.A., Malmirchegini, G.R., Spirig, T., Mackay, J.P., Clubb, R.T., Guss, J.M., and Gell, D.A. (2014)
    Molecular architecture of Staphylococcus aureus hemoglobin-receptor, IsdH, in complex with human hemoglobin
    J Biol Chem Accepted for publication, 9 Jan., 2014. #2013/545566,

  3. Vandevenne, M., Jacques, D.A., Arzur, C., Nguyen, C.D., Segal, D.J., Matthews, J.M., Crossley, M., Guss, J.M., and Mackay, J.P. (2013)
    New insights into DNA recognition by zinc fingers revealed by structural analysis of ZNF217
    J Biol Chem 288, 10616-10627.

  4. Stokes, P.H., Liew, C.W., Kwan, A.H., Foo, P., Barker, H.E., Djamirze, A., O'Reilly, V., Visvader, J.E., Mackay, J.P., and Matthews, J.M. (2013)
    Structural basis of the interaction of the breast cancer oncogene LMO4 with the tumour suppressor CtIP/RBBP8
    J. Mol. Biol. 425, 1101-1110.

  5. Silva, A.P.G., Kwan, A.H., and Mackay, J.P. (2013)
    Backbone and sidechain 1H, 13C and 15N chemical shift assignments of an N-terminal domain from human CHD4
    Biomol. NMR Ass. Epub ahead of print, 17 Feb, 2013,

  6. Mackay, J.P., Segal, D.J., and Crossley, M. (2013)
    Is there a telltale RH fingerprint in zinc fingers that recognize methylated CpG dinucleotides?
    Trends Biochem Sci 38, 421-2.

  7. Joseph, S., Kwan, A.H., Mackay, J.P., Cubeddu, L., and Matthews, J.M. (2013)
    Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1
    Biomol. NMR Ass. Epub ahead of print, 16 Feb, 2013,

  8. Gamsjaeger, R., O'Connell, M.R., Cubeddu, L., Shepherd, N.E., Lowry, J.A., Vandevenne, M., Swanton, M.K., Matthews, J.M., and Mackay, J.P. (2013)
    Structural analysis of DNA binding by Myelin Transcription Factor 1 double zinc fingers
    J Biol Chem 288, 35180-91.

  9. Gamsjaeger, R., Fernando, R., Bang, L.H., Touma, C., Nguyen, C.D., Matthews, J.M., Cubeddu, L., and Mackay, J.P. (2013)
    Semi-quantitative and quantitative analysis of protein-DNA interactions using steady-state measurements in Surface Plasmon Resonance competition experiments
    Anal Biochem 440, 178-85.

  10. Dickson, C.F., Rich, A.M., D'Avigdor, W.M.H., Collins, D.A., Lowry, J.A., Mollan, T.L., Khandros, E., Olsen, J.S., Weiss, M.J., Mackay, J.P., Lay, P.A., and Gell, D.A. (2013)
    Alpha-hemoglobin stabilizing protein (AHSP) perturbs the proximal heme pocket of oxy-alpha-hemoglobin and weakens the iron-oxygen bond
    J Biol Chem 288, 19986-20001.

  11. Cordina, N.M., Liew, C.K., Gell, D.A., Fajer, P.G., Mackay, J.P., and Brown, L.J. (2013)
    Effects of calcium binding and the hypertrophic cardiomyopathy mutation A8V on the dynamic equilibrium between the closed and open states of the regulatory N-domain of isolated cardiac Troponin C
    Biochemistry 52, 1950-1962.

  12. Campbell, A.E., Wilkinson-White, L., Mackay, J.P., Matthews, J.M., and Blobel, G.A. (2013)
    Dissecting molecular pathways that underlie disease-causing GATA1 mutations
    Blood 121, 5218-27.

  13. O'Connell, M.R., Vandevenne, M., Nguyen, C.D., Matthews, J.M., Gamsjaeger, R., Segal, D.J., and Mackay, J.P. (2012)
    Modular assembly of RanBP2-type zinc finger domains to target single-stranded RNA
    Angew Chem Int Ed Engl 51, 5371-5375.

  14. Mussleman, C.A., Ramirez, J., Sims, J.K., Mansfield, R.E., Oliver, S.S., Denu, J.M., Mackay, J.P., Wade, P.A., Hagman, J., and Kutateladze, T. (2012)
    Bivalent recognition of a single nucleosome by the tandem PHD fingers of CHD4 is required for CHD4-mediated repression
    Proc Natl Acad Sci USA 109, 787-792.

  15. Morris, V.K., Kwan, A.H., Mackay, J.P., and Sunde, M. (2012)
    Backbone and sidechain 1H, 13C and 15N chemical shift assignments of the hydrophobin DewA from Aspergillus nidulans
    Biomol. NMR Ass. 6, 83-86.

  16. McKenzie, J.L., Duyvestyn, J.M., Smith, T., Bendak, K., Mackay, J.P., Cursons, R., Cook, G.M., and Arcus, V.L. (2012)
    Determination of ribonuclease sequence specificity using Pentaprobes and mass spectrometry
    RNA 18, 1267-78.

  17. Macindoe, I., Kwan, A.H., Ren, Q., Morris, V.K., Yang, W., Mackay, J.P., and Sunde, M. (2012)
    Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS
    Proc Natl Acad Sci USA 109, 804-811.

  18. Hseih, Y.S.Y., Wilkinson, B.L., O'Connell, M.R., Mackay, J.P., Matthews, J.M., and Payne, R.J. (2012)
    Synthesis of the bacteriocin glycopeptide sublancin 168 and S-glycosylated variants
    Org Lett 14, 1910-1913.

  19. Dastmalchi, S., Wilkinson-White, L., Kwan, A.H., Gamsjaeger, R., Mackay, J.P., and Matthews, J.M. (2012)
    Solution structure of a tethered Lmo2(LIM2)/Ldb1(LID) complex
    Protein Science 21, 1768-74.

  20. Cordina, N.M., Liew, C.K., Gell, D.A., Fajer, P.G., Mackay, J.P., and Brown, L.J. (2012)
    Interdomain orientation of cardiac Troponin C characterized by Paramagnetic Relaxation Enhancement NMR reveals a compact state
    Protein Science in press, 20 June, 2012. Manuscript # PRO-12-0080,

  21. Burdach, J., O'Connell, M.R., Mackay, J.P., and Crossley, M. (2012)
    Two-timing zinc finger transcription factors liasing with RNA
    Trends Biochem Sci 37, 199-205.

  22. Bhati, M., Lee, C., Gadd, M.S., Jeffries, C.M., Kwan, A.H., Whitten, A., Trewhella, J., Mackay, J.P., and Matthews, J.M. (2012)
    Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions
    PloS One 7, e40719.

  23. Bendak, K., Loughlin, F.E., Cheung, V.M., O'Connell, M.R., Crossley, M., and Mackay, J.P. (2012)
    A rapid method for assessing the RNA-binding potential of a protein
    Nucl Acids Res 40, e105.

  24. Wißmüller, S., Font, J., Liew, C.W., Cram, E., Schroeder, T., Turner, J., Crossley, M., Mackay, J.P., and Matthews, J.M. (2011)
    Protein-protein interactions: analysis of a false positive GST pulldown result
    Proteins: Struct, Funct, Bioinf 79, 2365-71.

  25. Wilkinson-White, L., Gamsjaeger, R., Dastmalchi, S., Weinert, B., Stokes, P., Crossley, M., Mackay, J.P., and Matthews, J.M. (2011)
    The structural basis of simultaneous recruitment of LMO2 and FOG by GATA1
    Proc Natl Acad Sci USA 108, 14443-14448.

  26. Setiyaputra, S., Patrick, W., and Mackay, J.P. (2011)
    The structure of a truncated phosphoribosylanthranilate isomerase suggests a unified model for evolution of the (ba)8 barrel fold
    J Mol Biol 408, 291-303.

  27. Nunez, N., Clifton, M.M.K., Funnell, A.P.W., Artuz, C., Hallal, S., Quinlan, K.G.R., Font, J., Vandevenne, M., Setiyaputra, S., Pearson, R.C.M., Mackay, J.P., and Crossley, M. (2011)
    The Multi-Zinc Finger Protein Znf217 contacts DNA through two zinc fingers
    J Biol Chem 286, 38035-38042.

  28. Nguyen, C.D., Mansfield, R.E., Leung, W., Vaz, P.M., Loughlin, F.E., Grant, R.P., and Mackay, J.P. (2011)
    Characterization of a family of RanBP2-type zinc fingers that recognize single-stranded RNA
    J Mol Biol 407, 273-283.

  29. Mansfield, R.E., Mussleman, C.A., Kwan, A.H., Oliver, S.S., Garske, A.L., Davrazou, F., Denu, J.M., Kutateladze, T., and Mackay, J.P. (2011)
    The plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9
    J Biol Chem 286, 11779-11791.

  30. Mackay, J.P., Font, J., and Segal, D.J. (2011)
    The prospects for designer RNA-binding domains
    Nature Struct Mol Biol 18, 256-261.

  31. Liew, C.W., Kwan, A.H., Stokes, P.H., Mackay, J.P., and Matthews, J.M. (2011)
    1H, 15N and 13C assignments of an intramolecular LMO4-LIM1/CtIP complex
    Biomol. NMR Ass. 6, 31-34.

  32. Lejon, S., Thong, S.Y., Murthy, A., AlQarni, S., Murzina, N., Blobel, G.A., Laue, E.D., and Mackay, J.P. (2011)
    Insights into the association of the nucleosome remodeling and deacetylase (NuRD) complex with friend of GATA-1 (FOG-1) from the crystal structure of a retinoblastoma associated protein-48 (RbAp48) FOG-1 complex
    J Biol Chem 286, 1196-1203.

  33. Lamonica, J.M., Deng, W., Kadauke, S., Campbell, A.E., Gamsjaeger, R., Wang, H., Cheng, Y., Billin, A., Hardison, R.C., Mackay, J.P., and Blobel, G.A. (2011)
    The Double Bromodomain Protein Brd3 Associates with Acetylated GATA1 to Promote its Chromatin Occupancy at Erythroid Target Genes
    Proc Natl Acad Sci USA 108 159-168.

  34. Kwan, A.H., Mobli, M., Gooley, P.R., King, G.F., and Mackay, J.P. (2011)
    Macromolecular NMR spectroscopy for the non-spectroscopist. Part 1: The basics
    FEBS J 278, 687-703.

  35. Kumar, K.K., Jacques, D.A., Pishchany, G., Caradoc-Davies, T.T., Spirig, T., Malmirchegini, G.R., Langley, D.B., Dickson, C.F., Mackay, J.P., Clubb, R.T., Skaar, E.P., Guss, J.M., and Gell, D.A. (2011)
    The structural basis for haemoglobin capture by Staphylococcus aureus
    J Biol Chem 286, 38439-38447.

  36. Gamsjaeger, R., Webb, S.R., Lamonica, J.M., Billin, A., Blobel, G.A., and Mackay, J.P. (2011)
    Structural basis and specificity of transcription factor GATA1 recognition by BET-family bromodomain protein Brd3
    Mol. Cell. Biol. 31, 2632-2640.

  37. Bieri, M., Kwan, A.H., Mobli, M., King, G.F., Mackay, J.P., and Gooley, P.R. (2011)
    Macromolecular NMR spectroscopy for the non-spectroscopist. Part 2: Beyond macromolecular structure determination
    FEBS J 278, 704-715.

  38. Wilkinson-White, L.E., Dastmalchi, S., Kwan, A.H., Ryan, D.P., Mackay, J.P., and Matthews, J.M. (2010)
    1H, 15N and 13C assignments of an intramolecular Lmo2-LIM2/Ldb1-LID complex
    Biomol NMR Ass 4, 203-206.

  39. Schwalbe, M., Dutta, K., Libich, D.S., Venugobal, H., Claridge, J.K., Gell, D.A., Mackay, J.P., Edwards, P.J., and Pascal, S. (2010)
    Order-disorder Equilibria in the Leucine Zipper domain of Prostate Apoptosis Response Factor-4 (Par-4)
    Proteins: Struct., Funct. Bioinformat. 78, 2433-49.

  40. Kumar, K.K., Dickson, C.F., Weiss, M.J., Mackay, J.P., and Gell, D.A. (2010)
    Alpha-hemoglobin stabilizing protein (AHSP) stabilizes apo-alpha-hemoglobin in a partially folded state
    Biochem J 432, 275-282.

  41. Hynson, R.M.G., Kwan, A.H., Jacques, D.A., Mackay, J.P., and Trewhella, J. (2010)
    1H, 13C and 15N backbone and side chain resonance assignments of the N-terminal domain of the histidine kinase inhibitor KipI from Bacillus subtilis
    Biomol NMR Ass 4, 167-169.

  42. Talib, J., Beck, J.L., Urathamakul, T., Nguyen, C.D., Aldrich-Wright, J.R., Mackay, J.P., and Ralph, S.F. (2009)
    A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity
    Chem. Commun. Oct 7, 5546-5548.

  43. O'Connell, M.R., Gamsjaeger, R., and Mackay, J.P. (2009)
    The structural analysis of protein-protein interactions by NMR spectroscopy
    Proteomics 9, 5224-32.

  44. Musselman, C., Mansfield, R., Garske, A., Davrazou, F., Kwan, A., Oliver, S., OLeary, H., Denu, J., Mackay, J.P., and Kutateladze, T. (2009)
    Binding of the CHD4 PHD2 finger to histone H3 is modulated by covalent modifications
    Biochem J 423, 179-187.

  45. Matthews, J.M., Bhati, M., Mansfield, R.E., Lehtomaki, E., Cubeddu, L., and Mackay, J.P. (2009)
    It takes two to tango
    Curr Pharm Design 15, 3681-3696.

  46. Macindoe, I., Glockner, L., Vukasin, P., Stennard, F.A., Costa, M.W., Harvey, R.P., Mackay, J.P., and Sunde, M. (2009)
    Conformational stability and DNA binding specificity of the cardiac T-box transcription factor Tbx20
    J Mol Biol 389, 606-618.

  47. Lowry, J.A., Gamsjaeger, R., Thong, S., Broitman-Maduro, G., Matthews, J.M., Maduro, M., and Mackay, J.P. (2009)
    Structural analysis of MED-1 reveals unexpected diversity in the mechanism of DNA recognition by GATA-type zinc finger domains
    J Biol Chem 284, 5827-5835.

  48. Loughlin, F.E., Mansfield, R.E., Vaz, P.M., McGrath, A.P., Setiyaputra, S., Gamsjaeger, R., Chen, E.S., Morris, B.J., Guss, J.M., and Mackay, J.P. (2009)
    The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA binding domains that recognize 5´ splice site-like sequences
    Proc. Natl Acad. Sci. USA 106, 5581-5586.

  49. Gell, D.A., Feng, L., Zhou, S., Jeffrey, P.D., d'Avigdor, W., Gow, A., Weiss, M.J., Shi, Y., and Mackay, J.P. (2009)
    A cis-proline in alpha-hemoglobin stabilizing protein directs the structural reorganization of alpha-hemoglobin
    J Biol Chem 284, 29462-29469.

  50. Wilkinson, S.M., Liew, C.W., Mackay, J.P., Saleh, H.M., Withers, S.G., and McLeod, M.D. (2008)
    E. coli glucoronylsynthase: an engineered enzyme for the synthesis of glucoronides
    Org Lett 10, 1585-1588.

  51. Sunde, M., Kwan, A.H., Templeton, M.D., Beever, R.E., and Mackay, J.P. (2008)
    Structural analysis of hydrophobins
    Micron 39, 773-784.

  52. Matthews, J.M., Loughlin, F.E., and Mackay, J.P. (2008)
    Designed metal-binding sites in biomolecular and bioinorganic interactions
    Curr. Opin. Struct. Biol 18, 484-90.

  53. Mackay, J.P., Sunde, M., Lowry, J.A., Crossley, M., and Matthews, J.M. (2008)
    Protein interactions: to believe or not to believe?
    Trends in Biochem. Sci. 33, 242-243.

  54. Loughlin, F.E., Lee, M., Guss, J.M., and Mackay, J.P. (2008)
    Crystallization of a ZRANB2:RNA complex
    Acta Cryst. F 64, 1175-1177.

  55. Liew, C.K., Gamsjaeger, R., Mansfield, R.E., and Mackay, J.P. (2008)
    NMR spectroscopy as a tool for the rapid assessment of the conformation of GST-fusion proteins
    Protein Sci 17, 1630-1635.

  56. Langley, D.B., Shojaei, M., Chan, C., Lok, H.C., Mackay, J.P., Traut, T.W., Guss, J.M., and Christopherson, R.I. (2008)
    Structure and inhibition of orotidine 5'-monophosphate decarboxylate from Plasmodium falciparum
    Biochemistry 47, 3842-3854.

  57. Kwan, A.H., Macindoe, I., Vukasin, P.V., Morris, V.K., Kass, I., Gupte, R., Mark, A., Templeton, M.D., Mackay, J.P., and Sunde, M. (2008)
    The Cys3-Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity
    J Mol Biol 382, 708-720.

  58. Griffin, M.D.W., Dobson, R.C.J., Pearce, G., Antonio, L., Whitten, A.E., Liew, C.K., Mackay, J.P., Trewhella, J., Jameson, G.B., Perugini, M.A., and Gerrard, J.A. (2008)
    Evolution of quaternary structure in a homotetrameric protein
    J Mol Biol 380, 691-703.

  59. Gamsjaeger, R., Swanton, M.K., Kobus, F.J., Lehtomaki, E., Lowry, J., Matthews, J.M., and Mackay, J.P. (2008)
    Structural and biophysical analysis of Myelin Transcription factor 1 zinc fingers
    J Biol Chem 283, 5158-5167.

  60. Bhati, M., Lee, C., Nancarrow, A., Lee, M., Craig, V., Bach, I., Guss, J.M., Mackay, J.P., and Matthews, J.M. (2008)
    Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes
    EMBO J. 27, 2018-2029.

  61. Yu, X., Kong, Y., Dore, L.C., Abdulmalik, O., Katein, A.M., Zhou, S., Choi, J., Gell, D.A., Mackay, J.P., Gow, A.J., and Weiss, M.J. (2007)
    An erythroid chaperone that facilitates native folding of alpha globin subunits for hemoglobin synthesis
    J Clin Invest 117, 1856-1865.

  62. Wang, C.I., Guncar, G., Forwood, J.K., Teh, T., Catanzariti, A.M., Lawrence, G.J., Loughlin, F.E., Mackay, J.P., Schirra, H.J., Anderson, P.A., Ellis, J.G., Dodds, P.N., and Kobe, B. (2007)
    Crystal structures of flax rust avirulence proteins AvrL567-A and -D reveal details of the structural basis for flax disease resistance specificity
    The Plant Cell 19, 2898-2912.

  63. Porter, C., Matthews, J.M., Mackay, J.P., Pursglove, S.E., Schmidberger, P., Leedman, P., Pero, S.C., Krag, D.N., Wilce, M.C.J., and Wilce, J.A. (2007)
    Grb7 SH2 domain structure and interactions with a cyclic peptide inhibitor of cancer cell migration and proliferation
    BMC Struct. Biol. 7, 58.

  64. Mackay, J.P., Sunde, M., Lowry, J.A., Crossley, M., and Matthews, J.M. (2007)
    Protein interactions: is seeing believing?
    Trends Biochem. Sci. 32, 530-531.

  65. Liew, C.K., Crossley, M., Mackay, J.P., and Nicholas, H.R. (2007)
    Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)
    J. Mol. Biol. 366, 382-390.

  66. Kirk, E.P., Sunde, M., Costa, M.W., Rankin, S.A., Wolstein, O., Castro, M.L., Butler, T.L., Hyun, C., Guo, G., Otway, R., Mackay, J.P., Waddell, L.B., Cole, A.D., Hayward, C., Keogh, A., Macdonald, P., Griffiths, L., Fatkin, D., Sholler, G.F., Zorn, A.M., Feneley, M.P., Winlaw, D.S., and Harvey, R.P. (2007)
    Mutations in cardiac T-box factor TBX20 are associated with diverse cardiac pathologies including defects of septation and valvulogenesis, and cardiomyopathy
    Am J Human Genet 81, 280-91.

  67. Gamsjaeger, R., Liew, C.K., Loughlin, F.E., Crossley, M., and Mackay, J.P. (2007)
    Sticky fingers: zinc fingers as protein recognition motifs
    Trends Biochem Sci 32, 63-70.

  68. Ryan, D.P., Sunde, M., Kwan, A.H.-Y., Marianayagam, N.J., Nancarrow, A.L., vanden Hoven, R.N., Thompson, L.S., Baca, M., Mackay, J.P., Visvader, J.E., and Matthews, J.M. (2006)
    Identification of the key LMO2-binding determinants on Ldb1
    J. Mol. Biol. 359, 66-75.

  69. Lowry, J.A. and Mackay, J.P. (2006)
    GATA1: one protein, many partners
    Int. J. Biochem. Cell Biol. 38, 6-11.

  70. Loughlin, F.E. and Mackay, J.P. (2006)
    Zinc fingers are known as domains for binding DNA and RNA. Do they also mediate protein-protein interactions?
    IUBMB Life 58, 731-733.

  71. Liew, C.W., Rand, K.D., Simpson, R.J., Yung, W.W.M., Mansfield, R.E., Crossley, M., Proetorius-Ibba, M., Nerlov, C., Poulsen, F.M., and Mackay, J.P. (2006)
    Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1
    J Biol Chem 281, 28296-28306.

  72. Kwan, A.H.Y., Winefield, R.D., Sunde, M., Matthews, J.M., Templeton, M.D., and Mackay, J.P. (2006)
    Structural basis for rodlet assembly in fungal hydrophobins
    Proc. Natl Acad. Sci. USA 103, 3621-3626 Track II.

  73. Kook, H., Yung, W.W.M., Simpson, R.J., Lee, H.J., Shin, S., Lowry, J.A., Loughlin, F.E., Yin, Z., Epstein, J.A., and Mackay, J.P. (2006)
    Analysis of the structure and function of the transcriptional co-regulator HOP
    Biochemistry 45, 10584-90.

  74. Gell, D.A., Westman, B.J., Gorman, D., Liew, C.K., Welsh, J.J., Weiss, M.J., and Mackay, J.P. (2006)
    A novel haem-binding interface in the 22 kDa haem-binding protein p22HBP
    J. Mol. Biol. 362, 287-297.

  75. Weiss, M.J., Zhou, S., Feng, L., Gell, D.A., Mackay, J.P., Shi, Y., and Gow, A.J. (2005)
    Role of alpha hemoglobin stabilizing protein in normal erythropoiesis and b thalassemia
    Ann. New York Acad. Sci. 1054, 103-117.

  76. Sharpe, B.K., Liew, C.K., Wilce, J.A., Kwan, A.H.Y., Crossley, M., Matthews, J.M., and Mackay, J.P. (2005)
    Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting
    Structure 13, 257-266.

  77. Pursglove, S. and Mackay, J.P. (2005)
    CSL: a notch above the rest.
    Int. J. Biochem. Cell Biol. 34, 2472-7.

  78. Porter, C., Wilce, M., Mackay, J.P., Leedman, P., and Wilce, J.A. (2005)
    Grb7-SH2 domain dimerisation is affected by a single point mutation
    Eur. Biophys. J. 34, 454-60.

  79. Mackay, J.P., Muznieks, L., Toonkool, P., and Weiss, A.S. (2005)
    The hydrophobic domain 26 of human tropoelastin is unstructured in solution
    J. Struct. Biol. 150, 154-162.

  80. Liew, C.K., Simpson, R.J.W., Kwan, A.H., Crofts, L., Loughlin, F., Matthews, J., Crossley, M., and Mackay, J.P. (2005)
    Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction
    Proc. Natl Acad. Sci. USA 102, 583-588 Track II.

  81. Lee, C., Nancarrow, A.N., Bach, I., Mackay, J.P., and Matthews, J.M. (2005)
    1H, 15N and 13C assignments of an intramolecular Lhx3:ldb1 complex.
    J. Biomol. NMR 33, 198.

  82. Kwan, A.H., Fairley, K., Anderberg, P.I., Liew, C.W., Harding, M.M., and Mackay, J.P. (2005)
    Solution structure of a recombinant Type I antifreeze protein: the effects of supercooling and N-acetylation.
    Biochemistry 44, 1980-1988.

  83. Feng, L., Zhou, S., Gu, L., Gell, D.A., Mackay, J.P., Weiss, M.J., Gow, A.J., and Shi, Y. (2005)
    Structure of oxidized alpha-haemoglobin bound to AHSP reveals a protective mechanism for haem
    Nature 435, 697-701.

  84. Duggin, I.G., Wake, G., Matthews, J.M., Dixon, N.E., and Mackay, J.P. (2005)
    A complex mechanism controls polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis.
    J. Biol. Chem. 280, 13105-13.

  85. Westman, B.J., Perdomo, J., Matthews, J.M., Crossley, M., and Mackay, J.P. (2004)
    Structural studies on a protein-binding zinc-finger domain of Eos reveal both similarities and differences to classical ZnFs
    Biochemistry 43, 13318-13327.

  86. Sunde, M., McGrath, K.C.Y., Young, L., Matthews, J.M., Chua, E., Mackay, J.P., and Death, A. (2004)
    TC-1 is a novel tumorigenic and natively disordered protein associated with thyroid cancer
    Cancer Research 64, 2766-2773.

  87. Simpson, R.J.Y., Lee, S.H.Y., Bartle, N., E.Y., S., Visvader, J.E., Matthews, J.M., Mackay, J.P., and Crossley, M. (2004)
    A classical zinc finger from FOG mediates an interaction with the coiled-coil of TACC3.
    J. Biol. Chem. 279, 39789-39797.

  88. Kong, Y., Zhou, S., Kihm, A.J., Katein, A.M., Yu, X., Gell, D.A., Mackay, J.P., Adachi, K., Foster-Brown, L., Louden, C.S., Gow, A.J., and Weiss, M.J. (2004)
    Loss of alpha hemoglobin stabilizing protein (AHSP) impairs erythropoiesis and exacerbates b thalassemia
    J. Clin. Invest. 114, 1457-66.

  89. Feng, L., Gell, D.A., Zhou, S., Gu, L., Kong, Y., Li, J., Hu, M., Yan, N., Lee, C., Rich, A.M., Armstrong, R.S., Lay, P.A., Gow, A.J., Weiss, M.J., Mackay, J.P., and Shi, Y. (2004)
    Molecular mechanism of AHSP-mediated stabilization of a-hemoglobin
    Cell 119, 629-40.

  90. Donadini, R., Liew, C.W., Y, K.A.H., Mackay, J.P., and Fields, B.A. (2004)
    Crystal and solution structures of a superantigen from Yersinia pseudotuberculosis reveal a jelly-roll fold similar to viral capsid proteins and TNF-superfamily members
    Structure 12, 143-156.

  91. Dastmalchi, S., Church, W.B., Morris, M.B., Iismaa, T.P., and Mackay, J.P. (2004)
    Presence of transient helical segments in the galanin-like peptide evident from 1H NMR, circular dichroism, and prediction studies
    J Struct Biol 146, 261-71.

  92. Chow, M.K.M., Mackay, J.P., Whisstock, J.C., Scanlon, M.J., and Bottomley, S.P. (2004)
    Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3
    Biochem. Biophys. Res. Commun. 322, 387-394.

  93. Westman, B.J., Perdomo, J., Sunde, M., Crossley, M., and Mackay, J.P. (2003)
    The C-terminal domain of Eos forms a higher-order complex in solution
    J. Biol. Chem. 278, 42419-42426.

  94. Simpson, R.J.W., Cram, E.D., Czolij, R., Matthews, J.M., Crossley, M., and Mackay, J.P. (2003)
    CCHX zinc finger derivatives retain the ability to bind zinc and mediate protein-DNA interactions.
    J. Biol. Chem. 278, 28011-28018.

  95. Proudfoot, E.M., Mackay, J.P., and Karuso, P. (2003)
    Solution structure and behaviour of D-cis-a-[Ru(R,R-picchxnMe2)(phi)]2+ by NMR spectroscopy and molecular modeling
    J. Chem. Soc., Dalton Trans. 165-170.

  96. Plambeck, C.A., Kwan, A.H.Y., Adams, D., Westman, B.J., van der Weyden, L., Medcalf, R.L., Morris, B., and Mackay, J.P. (2003)
    The solution structure of the zinc finger domain from human splicing factor ZNF265
    J. Biol. Chem. 278, 22805-22811.

  97. Kwan, A.H.Y., Gell, D.A., Verger, A., Crossley, M., Matthews, J.M., and Mackay, J.P. (2003)
    Engineering a protein scaffold from a PHD finger
    Structure 11, 803-813.

  98. Kwan, A.H., Perdomo, J., Czolij, R., Mackay, J.P., and Crossley, M. (2003)
    Pentaprobe: a comprehensive sequence for the one-step detection of DNA-binding activities
    Nucl. Acids Res. 31, e124.

  99. Kook, H., Lepore, J., Gitler, A.D., Lu, M.M., Yung, W.W.M., Mackay, J.P., Zhou, R., Ferrari, V., Gruber, P., and Epstein, J.A. (2003)
    Cardiac hypertrophy and histone deacetylase-dependent transcriptional repression mediated by the atypical homeodomain protein Hop
    J. Clin. Invest. 112, 863-871.

  100. Deane, J.E., Mackay, J.P., Kwan, A.H.Y., Sum, E.Y., Visvader, J.E., and Matthews, J.M. (2003)
    Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4
    EMBO J. 22, 2224-2233.

  101. Westman, B.J., Mackay, J.P., and Gell, D.A. (2002)
    Ikaros, a key regulator of hematopoiesis
    Int. J. Biochem. Cell. Biol. 34, 1304-1307.

  102. Sharpe, B.K., Matthews, J.M., Kwan, A.H., Newton, A., Gell, D.A., Crossley, M., and Mackay, J.P. (2002)
    A new zinc binding fold underlines the versatility of zinc binding modules in protein evolution
    Structure 10, 639-48.

  103. Robson, S.A., Michie, K.A., Mackay, J.P., Harry, E., and King, G.F. (2002)
    The Bacillus subtilis cell division proteins FtsL and Div1C are intrinsically unstable and do not interact with one another in the absence of other septasomal components
    Mol. Microbiol. 44, 663-674.

  104. Pursglove, S.E., Mulhern, T.D., Mackay, J.P., Hinds, M.G., and Booker, G.W. (2002)
    The solution structure and intramolecular associations of the Tec kinase SH3 domain
    J. Biol. Chem. 277, 755-762.

  105. Polekhina, G., House, C.M., Traficante, N., Mackay, J.P., Sassoon, D.A., Parker, M.W., and Bowtell, D.D.L. (2002)
    The Siah Ubiquitin Ligase Component is Structurally Related to the TRAF family of Proteins and Modulates TNF-a-signalling
    Nature Struct. Biol. 9, 68-75.

  106. Leipinsh, E., Otting, G., Harding, M., Ward, L.G., Mackay, J.P., and Haymet, A.D.J. (2002)
    Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein
    Eur. J. Biochem. 269, 1259-1266.

  107. Kowalski, K., Liew, C.K., Matthews, J.M., Gell, D.A., Crossley, M., and Mackay, J.P. (2002)
    Characterization of the conserved interaction between GATA- and FOG-family proteins.
    J. Biol. Chem. 277, 35720-35729.

  108. Gell, D., Kong, Y., Eaton, S.A., Weiss, M.J., and Mackay, J.P. (2002)
    Biophysical characterization of the alpha-globin binding protein AHSP
    J Biol Chem 277, 40602-40609.

  109. Fairley, K., Westman, B.J., Pham, L.H., Haymet, A.D.J., Harding, M.M., and Mackay, J.P. (2002)
    Type I shorthorn sculpin antifreeze protein: recombinant synthesis, solution conformation and ice growth inhibition studies
    J. Biol. Chem. 277, 24073-24080.

  110. Deane, J.E., Visvader, J.E., Mackay, J.P., and Matthews, J.M. (2002)
    1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex.
    J. Biomol. NMR 23, 165-166.

  111. Chen, F., Kook, H., Milewski, R., Gitler, A., Lu, M.M., Li, J., Nazarian, R., Schnepp, R., Jen, K., Biben, C., Runke, G., Mackay, J.P., Novotny, J., Schwartz, R.J., Harvey, R.P., Mullins, M.C., and Epstein, J.A. (2002)
    Hop is an unusual homeobox gene that modulates cardiac development
    Cell 110, 713-723.

  112. Wang, X.-W., Connor, M., Wilson, D., Wilson, H., Nicholson, G.M., Smith, R., Shaw, D., Mackay, J.P., Alewood, P.F., Christie, M., and King, G.F. (2001)
    Discovery of a potent and highly specific peptide antagonist of insect calcium channels
    J. Biol. Chem. 276, 40306-40312.

  113. Proudfoot, E.M., Mackay, J.P., and Karuso, P. (2001)
    Site specificity of DNA-binding metalo-intercalators by NMR spectroscopy and molecular modeling
    Biochemistry 40, 4867-78.

  114. Newton, A., Mackay, J.P., and Crossley, M. (2001)
    The N-terminal zinc finger of the erythroid transcription factor GATA-1 binds GATC motifs in DNA
    J. Biol. Chem. 276, 35794-35801.

  115. Matthews, J.M., Visvader, J.A., and Mackay, J.P. (2001)
    1H, 15N and 13C assignments of FLIN2, an intramolecular LMO2:ldb1 complex
    J. Biomol. NMR 21, 385-386.

  116. Mackay, J.P., Matthews, J.M., Winefield, R.D., Mackay, L.G., Haverkamp, R.G., and Templeton, M.D. (2001)
    The hydrophobin EAS is largely unstructured in solution and functions by forming amyloid-like structures
    Structure 9, 83-91.

  117. Deane, J.E., Sum, E., Mackay, J.P., Lindeman, G.J., Visvader, J.E., and Matthews, J.M. (2001)
    Design, production and characterization of FLIN2 and FLIN4: the engineering of intramolecular LMO/ldb1 complexes
    Prot. Eng. 14, 493-499.

  118. Tchan, M.C., Choy, K.J., Mackay, J.P., Lyons, A.T.L., Bains, N.P.S., and Weiss, A.S. (2000)
    Interfacial Asn residues within an amide tetrad contribute to Max HLH-LZ homodimer stability
    J. Biol. Chem. 275, 37454-37461.

  119. Newton, A.L., Sharpe, B.S., Kwan, A., Mackay, J.P., and Crossley, M. (2000)
    The transactivation domain within cysteine/histidine-rich region 1 of CBP comprises two novel zinc-binding modules
    J. Biol. Chem. 275, 15128-15134.

  120. Matthews, J.M., Young, T.F., Tucker, S.P., and Mackay, J.P. (2000)
    The core of the respiratory syncytial virus fusion protein is a trimeric coiled coil
    J. Virol. 74, 5911-20.

  121. Matthews, J.M., Kowalski, K., Liew, C.K., Sharpe, B.K., Fox, A.H., Crossley, M., and Mackay, J.P. (2000)
    A class of zinc fingers involved in protein-protein interactions: biophysical characterization of CCHC fingers from FOG and U-shaped
    Eur. J. Biochem. 267, 1030-1038.

  122. Liew, C.K., Kowalski, K., Fox, A.H., Newton, A., Sharpe, B.K., Crossley, M., and Mackay, J.P. (2000)
    Solution structures of two CCHC zinc fingers from the FOG-family protein U-shaped that mediate protein-protein interactions.
    Structure 8, 1157-1166.

  123. Zerella, R., Evans, P.A., Ionides, J.M.C., Packman, L.C., Trotter, B.W., Mackay, J.P., and Williams, D.H. (1999)
    Autonomous folding of a peptide corresponding to the N-terminal b-hairpin from ubiquitin
    Protein Sci. 8, 1320-1331.

  124. Kowalski, K., Czolij, R., King, G.F., Crossley, M., and Mackay, J.P. (1999)
    The solution structure of the N-terminal zinc finger of GATA-1 reveals a specific binding face for the transcriptional co-factor FOG
    J. Biomol. NMR 13, 249-262.

  125. King, G.F., Rowland, S.L., Pan, B., Mackay, J.P., Mullen, G.P., and Rothfield, L.I. (1999)
    The dimerization and topological specificity functions of MinE reside in a structurally autonomous C-terminal domain
    Mol. Microbiol. 31, 1161-1169.

  126. Fox, A.H., Liew, C.K., Holmes, M., Kowalski, K., Mackay, J.P., and Crossley, M. (1999)
    Transcriptional cofactors of the FOG family interact with GATA proteins by means of multiple zinc fingers
    EMBO J. 18, 2812-2822.

  127. Crispino, J.D., Lodish, M.B., Mackay, J.P., and Orkin, S.H. (1999)
    Use of altered specificity mutants to probe a specific protein-protein interaction in differentiation: the GATA-1:FOG complex
    Mol. Cell 3, 219-228.

  128. Mackay, J.P., Kowalski, K., Fox, A.H., Czolij, R., King, G.F., and Crossley, M. (1998)
    Involvement of the N-finger in the self-association of GATA-1
    J. Biol. Chem. 273, 30560-30567.

  129. Mackay, J.P. and Crossley, M. (1998)
    Zinc fingers are sticking together
    Trends Biochem. Sci. 23, 1-4.

  130. Harding, M.M., Mokdsi, G., Mackay, J.P., Prodigalidad, M., and Lucas, S.W. (1998)
    Interactions of the antitumor agent molybdocene dichloride with oligonucleotides
    Inorg. Chem. 37, 2432-2437.

  131. Fox, A.H., Kowalski, K., King, G.F., Mackay, J.P., and Crossley, M. (1998)
    Key residues characteristic of GATA N-fingers are recognized by FOG
    J. Biol. Chem. 273, 33595-33603.

  132. Diefenbach, R.J., Mackay, J.P., Armati, P.J., and Cunningham, A.L. (1998)
    The C-terminal region of the stalk domain of ubiquitous human kinesin heavy chain contains the binding site for kinesin light chain
    Biochemistry 37, 16663-16670.

  133. Proudfoot, E.M., Mackay, J.P., Vagg, R.S., Vickery, K.A., Williams, P.A., and Karuso, P. (1997)
    delta-Cis-alpha-[Ru(R,R-picchxnMe2)(phen)]2+ shows minor groove AT selectivity with oligonucleotides
    Chem. Commun. 17, 1623-1624.

  134. Harding, M.M., Krippner, G.Y., Shelton, C.J., Rodger, A., Sanders, K.J., Mackay, J.P., and Prakash, A.S. (1997)
    DNA-binding studies of XSPTSPSZ, derivatives of the intercalating heptad repeat of RNA polymerase II
    Biopolymers 42, 387-398.

  135. Fletcher, J.I., Chapman, B.E., Mackay, J.P., Howden, M.E.H., and King, G.F. (1997)
    The structure of versutoxin (delta-atracotoxin-Hv1) provides insights into the binding of site 3 neurotoxins to the voltage-gated sodium channel
    Structure 5, 1525-1535.

  136. Dingley, A.J., Mackay, J.P., Shaw, G.L., Hambly, B.D., and King, G.F. (1997)
    Measuring macromolecular diffusion using heteronuclear multiple-quantum pulsed-field-gradient NMR
    J. Biomol. NMR 10, 1-8.

  137. Bains, N.P.S., Wilce, J.A., Heuer, K.H., Tunstall, M., Mackay, J.P., Bennett, M.R., Weiss, A.S., and King, G.F. (1997)
    Zipping up transcription factors: rational design of anti-Jun and anti-Fos peptides
    Lett. Pept. Sci. 4, 67-77.

  138. Mackay, J.P., Shelton, C.J., and Harding, M.M. (1996)
    Assignment of the 1H NMR spectrum and solution conformation of the antitumor antibiotic ditrisarubicin B
    Tetrahedron 52, 5617-24.

  139. Mackay, J.P., Shaw, G.L., and King, G.F. (1996)
    Backbone Dynamics of the c-Jun Leucine Zipper: 15N NMR Relaxation Studies
    Biochemistry 35, 4867-77.

  140. Heuer, K.H., Mackay, J.P., Podzebenko, P., Bains, N.P.S., Weiss, A.S., King, G.F., and Easterbrook-Smith, S.B. (1996)
    Development of a Sensitive Peptide-Based Immunoassay: Application to Detection of the Jun and Fos Oncoproteins
    Biochemistry 35, 9069-9075.

  141. Junius, F.K., Mackay, J.P., Bubb, W.A., Jensen, S.A., Weiss, A.S., and King, G.F. (1995)
    Nuclear magnetic resonance characterization of the Jun leucine zipper domain: unusual properties of coiled-coil interfacial polar residues
    Biochemistry 34, 6164-74.

  142. Dingley, A.J., Mackay, J.P., Chapman, B.E., Morris, M.B., Kuchel, P.W., Hambly, B.D., and King, G.F. (1995)
    Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2
    J. Biomol. NMR 6, 321-8.

  143. Williams, D.H., Searle, M.S., Westwell, M.S., Mackay, J.P., Groves, P., and Beauregard, D.A. (1994)
    The role of weak interactions, dimerization, and cooperativity in antibiotic action and biological signaling
    Chemtracts: Org. Chem. 7, 133-59.

  144. Williams, D.H., Searle, M.S., Groves, P., Mackay, J.P., Westwell, M.S., Beauregard, D.A., and Cristofaro, M.F. (1994)
    Functional roles of natural products: the involvement of extended arrays of weak interactions in cooperative binding phenomena
    Pure Appl. Chem. 66, 1975-82.

  145. Mackay, J.P., Gerhard, U., Beauregard, D.A., Westwell, M.S., Searle, M.S., and Williams, D.H. (1994)
    Glycopeptide Antibiotic Activity and the Possible Role of Dimerization: A Model for Biological Signaling
    J. Am. Chem. Soc. 116, 4581-90.

  146. Mackay, J.P., Gerhard, U., Beauregard, D.A., Maplestone, R.A., and Williams, D.H. (1994)
    Dissection of the Contributions toward Dimerization of Glycopeptide Antibiotics
    J. Am. Chem. Soc. 116, 4573-80.

  147. Groves, P., Searle, M.S., Mackay, J.P., and Williams, D.H. (1994)
    The structure of an asymmetric dimer relevant to the mode of action of the glycopeptide antibiotics
    Structure 2, 747-54.

  148. Williams, D.H., Searle, M.S., Mackay, J.P., Gerhard, U., and Maplestone, R.A. (1993)
    Toward an estimation of binding constants in aqueous solution: Studies of associations of vancomycin group antibiotics
    Proc. Natl. Acad. Sci. U. S. A. 90, 1172-8.

  149. Gerhard, U., Mackay, J.P., Maplestone, R.A., and Williams, D.H. (1993)
    The role of the sugar and chlorine substituents in the dimerization of vancomycin antibiotics
    J. Am. Chem. Soc. 115, 232-7.

Book chapters
  1. Gell, D.A., Grant, R.P., and Mackay, J.P. (2013)
    The detection and quantitation of protein oligomerization, in Protein dimerization in biology, J.M. Matthews, Editor 2013, Landes Bioscience: Austin, TX.

  2. Lehtomaki, E.M. and Mackay, J.P., Post-translational modifications in histone proteins, in Encycylopedia of Biophysics, Roberts, Editor 2012, Springer.

  3. Mansfield, R.E. and Mackay, J.P., SR-like protein ZRANB2, in Handbook of Metalloproteins, A. Messerschmidt, Editor 2010, John Wiley: Chichester.

  4. Mackay, J.P. and Segal, D.J., eds (2010)
    Engineered zinc finger proteins. 1 ed. Methods in Molecular Biology. Vol. 649. 2010, Springer. 440.

  5. Font, J. and Mackay, J.P. (2010)
    Classical zinc fingers as RNA-binding domains, in Engineered zinc finger proteins, J.P. Mackay and D.J. Segal, Editors. 2010, Humana

  6. Mansfield, R.E., Cross, A.E., Mackay, J.P., and Matthews, J.M. (2009)
    Protein recognition, in Amino Acids, Peptides and Proteins in Organic Chemistry. , A.B. Hughes, Editor 2009, Wiley

  7. Grant, R., Crossley, M., and Mackay, J.P. (2007)
    Zinc finger genes, in Encyclopedia of the Life Sciences 2007, John Wiley and Sons: Chichester.

  8. Gell, D.A. and Mackay, J.P. (2006)
    NMR spectroscopy in the analysis of protein-protein interactions, in Handbook of Modern Magnetic Resonance: Pharmaceutical Sciences, D. Craik, Editor 2006, Springer: Netherlands.



Lastest update: "News", on 28th Jul 2014.


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