Mackay & Matthews Lab

Protein structure, function and engineering lab

Research methods used in the lab

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14 May 2013: Lab retreat done and dusted for 2013

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In the course or our research, we use a wide range of experimental methods. Our philisophy is very much one of finding the right method to answer the questions we are interested in. Most of the methods that we use routinely are listed below.

Molecular biology (subcloning, mutagenesis)
Protein expression and purification
Yeast two-hybrid
Mammalian cell culture
GST-pulldowns
Gel shift assays (DNA + RNA)
SELEX
Phage display
Circular dichroism spectropolarimetry
Analytical ultracentrifugation
Surface plasmon resonance (Biacore 3000)
Mass spectrometry (MALDI and ESI)
Isothermal titration calorimetry
Differential scanning calorimetry
Multidimensional NMR spectroscopy

Lastest update: "Homepage", on 05th Jun 2013.

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