Protein structure, function and engineering lab Research methods used in the lab

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Recent news

16 Nov, 2015: Ana's paper comes out in JBC

9 Nov, 2015: ARC grant success

25 Sept, 2015: Sarah is now officially Dr Sarah.

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In the course or our research, we use a wide range of experimental methods. Our philisophy is very much one of finding the right method to answer the questions we are interested in. Most of the methods that we use routinely are listed below.
  • Molecular biology
  • Protein expression and purification
  • Yeast two-hybrid
  • Mammalian cell culture
  • GST-pulldowns
  • Gel shift assays (DNA + RNA)
  • Phage display
  • Circular dichroism spectropolarimetry
  • Analytical ultracentrifugation
  • Surface plasmon resonance (Biacore 3000)
  • Mass spectrometry (MALDI and ESI)
  • Isothermal titration calorimetry
  • Differential scanning calorimetry
  • Microscale thermophoresis
  • Multidimensional NMR spectroscopy
  • X-ray crystallography

Lastest update: "News", on 16th Nov 2015.

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© W. Yung 2002
School of MB